合成生物学时代基于非模式细菌的工业底盘细胞研究现状与展望

工业微生物底盘细胞的开发将为工业生物技术的发展提供优良的细胞工厂,有利于实现环境保护及经济可持续发展.基于合成生物学"设计-构建-测试-学习"(Design-Build-Test-Learn,DBTL)策略,对底盘细胞进行多维度的理性或半理性改造是实现"建物致知"以及"建物致用"目标的重要手段.文中简述了合成生物学DB...     

城市污水处理生态系统能值分析

脱水污泥的污染问题影响了城市污水处理厂的生存和发展,中水的不同处置方式对当地的水资源和水环境影响也不一样,将污水处理、脱水污泥和中水处置结合起来构建污水处理生态系统有利于研究污水处理的综合效益、探索促进污水处理事业可持续发展的途径.能值分析方法可作为分析这一复合系统的工具,传统能值分析方法由于对废物处理处置和排放的影响没有考虑,因此提出了针对污水处理生态系统的改进的能值分析方法和指标体系,以明镜滩污水处理生态系统作为案例用两种方法进行了对比分析.结果表明,改进的能值分析方法更适合于污水处理生态系统的实际情况;不可更新资源能值流比值(NR)和废物排放能值流比值(WR)对污水处理生态系统的可持续性影响最大,其次是中水回用能值流比值(IR),污水处理生态系统的可持续性最终取决于NR、WR和IR三者的共同贡献;废物利用可提高污水处理生态系统的可持续性.不同废物利用方式由于对废物利用的程度不同以及对不可更新资源的消耗不同,因此对污水处理生态系统的可持续性影响不一样;在提出的8个方案中,"脱水污泥填埋 中水排放"方案的可持续性最低,而"脱水污泥制堆肥 中水回用"方案的可持续性最高.     

Using Nested Average Electricity Allocation Protocols to Characterize Electrical Grids in Life Cycle Assessment

This study explored the impacts of electricity allocation protocols on the life cycle greenhouse gas (GHG) emissions of electricity consumption. The selection of appropriate electricity allocation protocols, methodologies that assign pools of electricity generators to electricity consumers, has not been well standardized. This can lead to very different environmental profiles of similar, electricity‐intensive processes. In an effort to better represent the interconnected nature of the U.S. electrical grid, we propose two new protocols that utilize inter‐regional trade information and localized emission factors to combine generating pools that are sub‐ or supersets of one another. This new nested approach increases the likelihood of capturing important inter‐regional electricity trading and the appropriate assignment of generator emissions to consumers of local and regional electricity. We applied the new and existing protocols to the U.S. primary aluminum industry, an industry whose environmental impact is heavily tied to its electricity consumption. Our analysis found GHG emission factors that were dramatically different than those reported in previous literature. We calculated production‐weighted average emission factors of 19.0 and 19.9 kilograms carbon dioxide equivalent per kilogram of primary aluminum ingot produced when using our two nested electricity allocation protocols. Previous studies reported values of 10.5 and 11.0, at least 42% lower than those found by our study.     

Urban Metabolism

Urban metabolism studies have been established for only a few cities worldwide, and difficulties obtaining adequate statistical data are universal. Constraints and peculiarities call for innovative methods to quantify the materials entering and leaving city boundaries. Such methods include the extrapolation of data at the country or the region level based, namely, on sales, population, commuters, workers, and waste produced.
The work described in this article offers a new methodology developed specifically for quantifying urban material flows, making possible the regular compilation of data pertinent to the characterization of a city's metabolism. This methodology was tested in a case study that characterized the urban metabolism of the city of Lisbon by quantifying Lisbon's material balance for 2004. With this aim, four variables were characterized and linked to material flows associated with the city: absolute consumption of materials/products per category, throughput of materials in the urban system per material category, material intensity of economic activities, and waste flows per treatment technology.
Results show that annual material consumption in Lisbon totals 11.223 million tonnes (20 tonnes per capita), and material outputs sum 2.149 million tonnes. Nonrenewable resources represent almost 80% of the total material consumption, and renewables consumption (biomass) constitutes only 18% of the total consumption. The remaining portion is made up of nonspecified materials.
A seemingly excessive consumption amount of nonrenewable materials compared to renewables may be the result of a large investment in building construction and a significant shift toward private car traveling, to the detriment of public transportation.     

太子参超低温脱毒及规模化组培育苗技术

以太子参茎尖为外植体,采用超低温去除病毒方法,通过组织培养研究了芽增殖诱导、丛生芽诱导、生根壮苗诱导的适合条件,以期形成太子参规模化育苗技术。结果表明：超低温处理1 h后,太子参脱毒率可达90%以上;规模化组培育苗最适宜配方为初代培养基为改良MS＋2.5 mg/L 6-BA＋0.5 mg/L IAA继代增殖培养基为改良MS＋1.2 mg/L KT＋0.5 mg/L NAA,壮苗生根培养基为改良MS＋0.2 mg/L KT＋1.5 mg/L DA-6＋10%蛋白粉。     

A Material Flow Accounting Case Study of the Lisbon Metropolitan Area using the Urban Metabolism Analyst Model

This article describes a new methodological framework to account for urban material flows and stocks, using material flow accounting (MFA) as the underlying method. The proposed model, urban metabolism analyst (UMAn), bridges seven major gaps in previous urban metabolism studies: lack of a unified methodology; lack of material flows data at the urban level; limited categorizations of material types; limited results about material flows as they are related to economic activities; limited understanding of the origin and destination of flows; lack of understanding about the dynamics of added stock; and lack of knowledge about the magnitude of the flow of materials that are imported and then, to a great extent, exported. To explore and validate the UMAn model, a case study of the Lisbon Metropolitan Area was used. An annual time series of material flows from 2003 to 2009 is disaggregated by the model into 28 material types, 55 economic activity categories, and 18 municipalities. Additionally, an annual projection of the obsolescence of materials for 2010–2050 was performed. The results of the case study validate the proposed methodology, which broadens the contribution of existing urban MFA studies and presents pioneering information in the field of urban metabolism. In particular, the model associates material flows with economic activities and their spatial location within the urban area.     

A Burning Issue: Rethinking the Transition from Hunter‐Gatherer to Industrial Sociometabolic Regimes

Hunter‐gatherers are commonly seen as having a fundamentally different sociometabolic regime from agrarian and industrial societies because they are thought to directly appropriate the products of natural ecosystems without modifying those systems in order to enhance their productivity. However, ethnographic and archeological evidence reveals that many hunter‐gatherers extensively employed fire to manage their ecosystems so as to increase production of desirable wild resources, thus engaging in “colonization of nature” that is not qualitatively different from that practiced by other types of society. They systematically burned wild vegetation in order to increase populations of edible wild plants consumed by humans and promote growth of forage for game animals. Deliberate ecosystem burning by Australian Aborigines represented an energy expenditure of 1,512 gigajoules per capita per year (GJ/capita/yr), a level of energy use that is more than three times higher than the United States (445 GJ/capita/yr). It is their profligate consumption of biomass energy that explains why the quality of life of many hunter‐gatherers was often better than that of traditional settled peasant farmers. Hence, the extent to which hunter‐gatherers have a distinct type of sociometabiolic regime is called into question. It can be argued that in the course of social evolution, there have been only two sociometabolic regimes. In one type, which includes hunter‐gatherers, swidden agriculturalists, and industrial societies, extrasomatic energy does most of the productive work, whereas in the other type, that of premodern settled agriculturalists, production is largely dependent on human muscle power.     

Using an Industrial Waste Account to Facilitate National Level Industrial Symbioses by Uncovering the Waste Exchange Potential

The identification of potential by‐product exchanges is important for fostering industrial symbiosis. To discover these potential exchanges, this article extends the analysis of local industrial symbiosis to a national scale. A waste input‐output table, which is a material flow accounting tool, was compiled and used as a database to examine the existing exchanges of by‐products. The supplies and demands of industrial wastes or by‐products were compared to highlight their potential use for promoting higher exchange flows. The analysis of the linkages indicated that the majority of each of the by‐products were reused by the few industries that had the technology and operational capacity for reuse. This finding is useful for determining which industries are good candidates for promoting further industrial symbiosis (IS). Based on a nation‐wide analysis that considered the industrial characteristics of Taiwan comprehensively, 23 types of major by‐products with greater reuse flows and 216 potential exchange patterns were identified between the industries. In addition, three types of eco‐industrial networks were characterized as follows according to their dominant types: (1) fossil fuel, metal, and mineral‐dominated; (2) agricultural and synthetic material‐dominated; and (3) information and communications technology (ICT) and chemical industry‐dominated eco‐industrial networks. This analysis highlights the resource exchange potentials and provides information to new firms for networking with existing businesses.     

Phylogenetic analysis of Bacteroidales 16S rRNA gene sequences from human and animal effluents and assessment of ruminant faecal pollution by real‐time PCR

Aims: The aims of this study were to evaluate the host‐specific distribution of Bacteroidales 16S rRNA gene sequences from human‐ and animal‐related effluents and faeces, and to define a ruminant‐specific marker. Methods and Results: Bacteroidales 16S rRNA gene clone libraries were constructed from samples of effluent (sewage, bovine manure and pig slurry) and faeces (human, bovine, pig and wild bird), using PCR primers targeting order Bacteroidales. The phylogenetic analysis revealed six main distinct human‐, bovine‐, pig‐ and wild bird‐specific clusters. From the bovine‐specific cluster II, we designed a ruminant‐specific marker, Rum‐2‐Bac, and this showed 97% sensitivity (n = 30) and 100% specificity (n = 40) when tested by TaqMan^® real‐time PCR. Average concentrations of this marker in bovine and sheep faeces and in bovine manure were 8·2 ± 0·5, 8·4 ± 1·3 and 7 ± 0·5 log₁₀ copies per gram, respectively. It was also quantified in samples of runoff water impacted by bovine manure, with average concentrations of 5·1 ± 0·3 log₁₀ copies per millilitre water. Conclusions: Our results confirmed that some members of Bacteroidales isolated from effluents and faeces had host‐specific distributions. Identification of a bovine‐specific cluster made it possible to design a reliable ruminant‐specific marker. Significance and Impact of the Study: The host‐specific distribution of Bacteroidales sequences from effluents mirrored the host‐specific distribution of sequences observed in individual faeces. This efficient new ruminant‐specific Bacteroidales 16S rRNA marker represents a useful addition to the microbial source tracking toolbox.     

Peak cement‐related CO2 emissions and the changes in drivers in China

In order to fight against the climate change, China has set a series of emission reduction policies for super‐emitting sectors. The cement industry is the major source of process‐related emissions, and more attention should be paid to this industry. This study calculates the process‐related, direct fossil fuel–related, and indirect electricity‐related emissions from China's cement industry. The study finds that China's cement‐related emissions peaked in 2014. The emissions are, for the first time, divided into seven parts based on the cement used in different new building types. The provincial emission analysis finds that developed provinces outsourced their cement capacities to less developed regions. This study then employs index decomposition analysis to explore the drivers of changes in China's cement‐related emissions. The results show that economic growth was the primary driver of emission growth, while emission intensity and efficiency were two offsetting factors. The changes in the construction industry's structure and improvement in efficiency were the two major drivers that contributed to the decreased emissions since 2014.