全文获取类型
收费全文 | 121篇 |
免费 | 3篇 |
国内免费 | 2篇 |
出版年
2021年 | 3篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 3篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 5篇 |
2013年 | 6篇 |
2011年 | 13篇 |
2010年 | 6篇 |
2009年 | 8篇 |
2008年 | 13篇 |
2007年 | 5篇 |
2006年 | 8篇 |
2005年 | 5篇 |
2004年 | 3篇 |
2003年 | 7篇 |
2002年 | 2篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1997年 | 1篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有126条查询结果,搜索用时 109 毫秒
41.
James R. Spotila Kenneth M. Terpin Robert R. Koons Ralph L. Bonati 《Environmental Biology of Fishes》1979,4(3):281-307
Synopsis Literature on the temperature requirements of fishes expected to occur in eastern Lake Erie and the upper Niagara River is
reviewed. Seventy-four species of fishes are reported from Lake Erie and sixty-one from the upper Niagara River. Incipient
upper lethal temperatures range from 23° C for Salmo trutta to 41° C for Carassius auratus and Ictalurus nebulosus. Preferred temperatures ranged from 10° C for Coregonus clupeaformis to 31.1° C for Lepomis macrochirus. Spawning temperatures range from < 3.8° C for C. artedii to 15.6–27.7° C for Alosa pseudoharengus. Data is discussed in terms of the effects of thermal effluents on individuals of a species, structure of aquatic communities
and impact on ecosystems. Synergistic effects of temperature and toxicants and disruption of sprawning are potentially the
most damaging direct effects of thermal effluents.
Heated water may be contributing to the present rate of eutrophication in the lake and river. Increased input of thermal effluents
into the eastern basin of Lake Erie will maintain a stress on the fishery and may irreversibly damage it. 相似文献
42.
Phosphorylation of silk fibroins improves the cytocompatibility of silk fibroin derived materials: A platform for the production of tuneable material
下载免费PDF全文
![点击此处可从《Biotechnology journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Vadim Volkov Andreia Vasconcelos Marisa P. Sárria Andreia C. Gomes Artur Cavaco‐Paulo 《Biotechnology journal》2014,9(10):1267-1278
Silk fibroin demonstrates great biocompatibility and is suitable for many biomedical applications, including tissue engineering and regenerative medicine. Current research focuses on manipulating the physico‐chemical properties of fibroin, and examining the effect of this manipulation on firobin's biocompatibility. Regenerated silk fibroin was modified by in vitro enzymatic phosphorylation and cast into films. Films were produced by blending, at several ratios, the phosphorylated and un‐phosphorylated fibroin solutions. Fourier transform infra‐red spectroscopy was used to determine the specific P–OH vibration peak, confirming the phosphorylation of the regenerated silk fibroin solution. Differential scanning calorimetry showed that phosphorylation altered the intra‐ and inter‐molecular interactions. Further experiments demonstrated that phosphorylation can be used to tailor the hydrophylicity/hydrophobicity ratio as well as the crystalinity of silk fibroin films. Release profiling of a model drug was highly dependent on silk modification level. Cytotoxicity assays showed that exposure to lixiviates of phosphorylated films only slightly affected cellular metabolism and proliferation, although direct contact resulted in a strong direct correlation between phosphorylation level and cell proliferation. This new method for tuning silk biomaterials to obtain specific structural and biochemical features can be adapted for a wide range of applications. Phosphorylation of silk fibroins may be applied to improve the cytocompatibility of any silk‐based device that is considered to be in contact with live animals or human tissues. 相似文献
43.
In this study, the performance of the anoxic filter bed and biological wriggle bed-ozone biological aerated filter (AFB-BWB-O3-BAF) process treating real textile dyeing wastewater was investigated. After more than 2 month process operation, the average effluent COD concentration of the AFB, BWB, O3-BAF were 704.8 mg/L, 294.6 mg/L and 128.8 mg/L, with HRT being 8.1-7.7 h, 9.2 h and 5.45 h, respectively. Results showed that the effluent COD concentration of the AFB decreased with new carriers added and the average removal COD efficiency was 20.2%. During operation conditions, HRT of the BWB and O3-BAF was increased, resulting in a decrease in the effluent COD concentration. However, on increasing the HRT, the COD reduction capability expressed by the unit carrier COD removal loading of the BWB reactor increased, while that of the O3-BAF reactor decreased. This study is a beneficial attempt to utilize the AFB-BWB-O3-BAF combine process for textile wastewater treatment. 相似文献
44.
L. Gebrati M. El Achaby H. Chatoui M. Laqbaqbi J. El Kharraz F. Aziz 《Saudi Journal of Biological Sciences》2019,26(7):1753-1757
Textile industry represents an important source of toxic substances rejected in environment. Indeed, effluent of these industries contains dyes and chemicals. They are rejected in environment without any treatment. The aim of this work is to evaluate ecotoxicological effect of industrial textile effluents on the sludge harvested from activated sludge treatment plant of Marrakech city (Morocco). For this, we are interested in determining the inhibition condition that corresponds to 50% decrease of bacterial activity in sludge. Obtained results showed that inhibition percentage of bacterial activity depends narrowly on contact time and on added effluent volume, until a limit concentration where there is no degradation of substratum. In fact, substratum degradation speed shows about 65 times decrease when 80% (v/v) of textile wastewater is added, in comparison with the controlled one. Consequently the inhibition constant (Ki) that corresponds to 50% of bacterial inhibition activity is estimated to 0.65 mg l?1 of dye. These studies confirm a real ecotoxicological risk of these effluents. Therefore, a treatment is mandatory before their rejection in environment. 相似文献
45.
M. Safiur Rahman Narottam Saha A. Hossain Molla Sharif M. Al-Reza 《Soil & Sediment Contamination》2014,23(4):353-373
Heavy metals (Pb, Cd, Ni, Cr, Cu, Zn, Mn, and As) concentration was investigated in the industrial effluents, water, sediment, and fish samples collected around the Dhaka Export Processing Zone, Savar, Bangladesh, to evaluate the level of contamination. The metals concentration in the industrial effluents of DEPZ and in the water samples of Dhalaibeel (lowland cum lake) and Bangshi River were significantly higher compared to the guideline values for industrial effluents and for drinking water (WHO and USEPA), respectively. The sedimentary metal concentrations were found to be lower than the respective probable effect concentrations (PECs) following the sediment quality guidelines. Furthermore, in comparison with the fish standards, the studied fish species were not found to be contaminated by heavy metals. Principal component analysis and cluster analysis demonstrated that the wastewater from the numerous industries and the domestic sewages around the DEPZ might have a possible impact on heavy metals contamination in the study area. The Pearson correlation analysis showed significant correlations (p < 0.01 and p < 0.05) between most of the metals in the samples of effluents, water, sediments, and fish muscles. The percentage enrichment factor (EF%) and geo-accumulation index () were followed to evaluate metal contamination in the sediment samples. Dhalaibeel sediment was maximally enriched for Cr (53.55%) and Bangshi River sediment for Zn (54.37%). The geo-accumulation index values for the sediment samples were less than zero, indicating that the sediment samples were free from contamination. This study could be used as a model study to assess the impact of anthropogenic activities on heavy metals contamination in aquatic ecosystems. 相似文献
46.
Dubé E Shareck F Hurtubise Y Beauregard M Daneault C 《Journal of industrial microbiology & biotechnology》2008,35(10):1123-1129
Colored wastewater from textile industries is a consequence of dye manufacturing processes. Two percent of dyes that are produced are discharged directly in aqueous effluent and more than 10% are subsequently lost during the textile coloration process. It is not surprising that these compounds have become a major environmental concern. In that context, we have evaluated the potential use of Streptomyces coelicolor laccase for decolourization of various dyes with and without a mediator. Results showed that in all cases the combination of laccase and the mediator acetosyringone was able to rapidly decolourize, to various degrees, all the dyes tested. In 10 min, decolourization was achieved at 94% for acid blue 74, 91% for direct sky blue 6b and 65% for reactive black 5. Furthermore, decolourization was achieved at 21% for reactive blue 19 and at 39% for the direct dye Congo red in 60 min. These results demonstrate the potential use of this laccase in combination with acetosyringone, a natural mediator, for dye decolourization. 相似文献
47.
Polyvinylamine‐coated polyester fibers as a carrier matrix for the immobilization of peroxidases
下载免费PDF全文
![点击此处可从《Engineering in Life Science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Biocatalytic transformations that employ immobilized enzymes become increasingly important for industrial applications. Synthetic or natural textile fiber materials such as polyester, polyamide or viscose are support materials that are comparatively inexpensive. Contrary to traditional support materials, their flexibility enables their use in reactors of any geometry and a fast and residue‐free removal from batch reactors. In this study a permanently immobilized peroxidase (Baylase®) has been investigated on polyester felt as a solid support as a new heterogeneous catalyst system. The polyester felt was functionalized by coating with polyvinylamine and subsequent activation with glutaraldehyde as a crosslinking agent. The enzyme load on the textile surface, the activity of the immobilized protein after repeated use as well as the storage stability was evaluated. Scanning electron micrographs and UV Vis spectroscopy made it possible to verify the enzyme immobilization on the textile surface. Furthermore, the load of immobilized peroxidase was determined by ICP OE spectrometry to be 9–12 mg per gram of textile. The activity of immobilized Baylase® remained high over 35 reaction cycles and a storage period of 8 weeks. 相似文献
48.
Eugenia J. Olguín Sonia Galicia Gabriel Mercado Teresa Pérez 《Journal of applied phycology》2003,15(2-3):249-257
An evaluation was made of the annual productivity of Spirulina (Arthrospira) and its ability to remove nutrients in outdoor raceways treating anaerobic effluents from pig wastewater under tropical conditions. The study was based at a pilot plant at La Mancha beach, State of Veracruz, Mexico. Batch or semi-continuous cultures were established at different seasons during four consecutive years. The protein content of the harvested biomass and the N and P removal from the ponds were also evaluated. Anaerobic effluents from digested pig waste were added in a proportion of 2% (v/v) to untreated sea-water diluted 1:4 with fresh water supplemented with 2 g L–1 sodium bicarbonate, at days 0, 3 and 5. A straight filament strain of Spirulina adapted to grow in this complex medium was utilized. A pH value 9.5 ± 0.2 was maintained. The productivity of batch cultures during summer 1998 was significantly more with a pond depth of 0.10 m than with a depth 0.065 m. The average productivity of semi-continuous cultures during summer 1999 was 14.4 g m–2 d–1 with a pond depth of 0.15 m and 15.1 g m–2 d–1 with a depth of 0.20 m. The average annual productivity for semi-continuous cultures operating with depths of 0.10 m for winter and 0.15 and 0.25 m for the rest of the year, was 11.8 g m–2 d–1. This is the highest value reported for a Spirulina cultivation system utilising sea-water. The average protein content of the semi-continuous cultures was 48.9% ash-free dry weight. NH4-N removal was in the range 84–96% and P removal in the range of 72–87%, depending on the depth of the culture and the season. 相似文献
49.
壳聚糖固定化真菌漆酶及其用于处理酚类污染物的研究 总被引:27,自引:0,他引:27
Trametessp. AH282在液体培养条件下经邻甲苯胺诱导能有效合成漆酶同工酶A。以壳聚糖为载体,戊二醛为交联剂进行了漆酶A的固定化研究,确定酶固定化适宜条件为:0.1g壳聚糖与15 mL 5%戊二醛交联8 h后,加入30.0U酶固定12h。在此条件下获得的固定化漆酶催化能力为176.4U/g载体,酶活回收率58.5%。与游离酶相比,固定化漆酶与作用底物愈创木酚的亲和力降低,但固定化酶的稳定性有明显改善。固定化漆酶的最适温度为55℃,比游离酶提高5℃;70℃条件下保温8 h,固定化酶保留酶活56.5%,而在相同条件下游离酶酶活明显下降。使用固定化漆酶反应装置进行酚类化合物转化实验,连续进行12批次操作,固定化酶酶活仍保持60%以上,漆酶使用效率明显提高。 相似文献
50.
Decolorization of textile dyes by enzymatic extract and submerged cultures of <Emphasis Type="Italic">Pleurotus sajor-caju</Emphasis> 总被引:1,自引:0,他引:1
Fernanda M. Munari Tamara A. Gaio Raquel Calloni Aldo J. P. Dillon 《World journal of microbiology & biotechnology》2008,24(8):1383-1392
Pleurotus sajor-caju PS2001 was screened in Petri dish plates to assess the dye-decolorizing ability of industrial textile dyes. P. sajor-caju PS2001 was also cultivated in solid-state fermentation containing sawdust of Pinus sp. and wheat bran to obtain the enzymatic extract, showing laccase and manganese-peroxidase activity, which was used to
test the capacity to degrade the textile dyes. Additional tests of decolorization were performed in liquid cultures. Anthraquinone-type
textile dyes proved to be substrates for the enzymatic system of P. sajor-caju PS2001. Cultures in Petri dish plates showed that the anthraquinone dye Reactive Blue 220 can act as a redox mediator for
the enzymatic reactions involved in the decolorization process, and enables the azo dye degradation. Reactive Blue 220 and
Acid Blue 280 were completely decolorized in 30 min and 60 min, respectively, during the tests with precipitated enzymatic
extract, while the azo dyes showed resistance to degradation. Additionally, in submerged cultures with dyes, veratryl alcohol
oxidases and lignin peroxidase activities were observed. These results suggest that the strain P. sajor-caju PS2001 has great potential for use in the bioremediation technology of recalcitrant pollutant such as textile effluents. 相似文献