Effect of protein deficiency on macroelement and trace element levels of weanling rats' small intestine and liver tissues

Protein energy malnutrition has become a major health issue in developing countries. In the present study, the effect of protein deficiency on the small intestine and liver tissue content of macroelements and trace elements was investigated in weanling rats. Forty-five male weanling Wistar albino rats were divided into three groups. The control group (C) was fed a standard diet containing 25% casein, whereas the two experimental groups E1 and E2 consumed 12% and 3% casein, respectively, over a period of 45 d. The tissue samples were analyzed for zinc, copper, iron, manganese, calcium, and magnesium by atomic absorption spectroscopy. The protein-deficient groups showed increased levels of iron in both tissues and decreased manganese in small intestine tissue from the E1 group. No other differences were found for the other elements. These results suggest that protein deficiency might cause iron accumulation in the liver and intestine and decreases of manganese in the small intestine.     

Impaired mitochondrial oxidative phosphorylation in skeletal muscle of the dystrophin-deficient mdx mouse

The mdx mouse, an animal model of the Duchenne muscular dystrophy, was used for the investigation of changes in mitochondrial function associated with dystrophin deficiency. Enzymatic analysis of skeletal muscle showed an approximately 50% decrease in the activity of all respiratory chain-linked enzymes in musculus quadriceps of adult mdx mice as compared with controls, while in cardiac muscle no difference was observed. The activities of cytosolic and mitochondrial matrix enzymes were not significantly different from the control values in both cardiac and skeletal muscles. In saponin-permeabilized skeletal muscle fibers of mdx mice the maximal rates of mitochondrial respiration were about two times lower than those of controls. These changes were also demonstrated on the level of isolated mitochondria. Mdx muscle mitochondria had only 60% of maximal respiration activities of control mice skeletal muscle mitochondria and contained only about 60% of hemoproteins of mitochondrial inner membrane. Similar findings were observed in a skeletal muscle biopsy of a Duchenne muscular dystrophy patient. These data strongly suggest that a specific decrease in the amount of all mitochondrial inner membrane enzymes, most probably as result of Ca²⁺ overload of muscle fibers, is the reason for the bioenergetic deficits in dystrophin-deficient skeletal muscle.     

Nutritional value of cannibalism and the role of starvation and nutrient imbalance for cannibalistic tendencies in a generalist predator

1. Cannibalism is considered an adaptive foraging strategy for animals of various trophic positions, including carnivores. However, previous studies on wolf spiders have questioned the high nutritional value of cannibalism. We therefore analysed two different aspects of nutritional quality of conspecifics in the wolf spider Pardosaprativaga: their value for survival, growth and development; and the growth efficiency of feeding on conspecifics. We also measured the propensity for cannibalistic attacks and the consumption rate of conspecifics in an experiment where hunger level and nutrient balance were manipulated. In all experiments, cannibalism was compared with predation on fruit flies as control prey. 2. The growth experiment gave ambiguous results regarding the nutritional quality of conspecifics. Spiders on pure cannibalistic diets split into two distinct groups, one performing much better and the other much worse than spiders on fruit fly diets. We discuss the possibility that the population is dimorphic in its cannibalistic propensity, with the latter group of individuals showing a high level of inhibition against cannibalistic attacks in spite of a high nutritional value of cannibalism. 3. The food utilization experiment confirmed the high nutritional quality of conspecifics, as cannibalistic spiders had the same growth rate as spiders fed insect prey in spite of a much lower consumption rate. 4. Inhibition against cannibalistic attacks was demonstrated in medium-sized juveniles: only half of the spiders attacked a prescribed victim of 50% the size of their opponents, and the latency for those that did attack was more than half an hour, compared with a few minutes for spiders fed fruit flies. 5. Nutrient-imbalanced spiders utilized an alternative insect diet less efficiently than balanced spiders, whereas no difference was present in efficiency of utilizing conspecifics. This result indicates that spiders can remedy at least part of a nutrient imbalance through cannibalism. 6. As spiders can escape nutritional imbalance as well as restore energy reserves through cannibalism, we predicted both nutrient imbalance and hunger to stimulate cannibalism. This prediction was confirmed only with respect to hunger. Nutrient-imbalanced spiders had reduced cannibalistic consumption, perhaps due to lowered predatory aggressiveness as a result of bad condition.     

Purification,cellular levels,and functional domains of lipase maturation factor 1

Over a third of the US adult population has hypertriglyceridemia, resulting in an increased risk of atherosclerosis, pancreatitis, and metabolic syndrome. Lipoprotein lipase (LPL), a dimeric enzyme, is the main lipase responsible for TG clearance from the blood after food intake. LPL requires an endoplasmic reticulum (ER)-resident, transmembrane protein known as lipase maturation factor 1 (LMF1) for secretion and enzymatic activity. LMF1 is believed to act as a client specific chaperone for dimeric lipases, but the precise mechanism by which LMF1 functions is not understood. Here, we examine which domains of LMF1 contribute to dimeric lipase maturation by assessing the function of truncation variants. N-terminal truncations of LMF1 show that all the domains are necessary for LPL maturation. Fluorescence microscopy and protease protection assays confirmed that these variants were properly oriented in the ER. We measured cellular levels of LMF1 and found that it is expressed at low levels and each molecule of LMF1 promotes the maturation of 50 or more molecules of LPL. Thus we provide evidence for the critical role of the N-terminus of LMF1 for the maturation of LPL and relevant ratio of chaperone to substrate.     

Sugar repression of photosynthesis: the role of carbohydrates in signalling nitrogen deficiency through source:sink imbalance

The aim of this work was to examine whether carbohydrates are involved in signalling N deficiency through source:sink imbalance. Photosynthetic metabolism in tobacco was studied over 8 d during the withdrawal of N from previously N-sufficient plants in which the source:sink ratio was manipulated by shading leaves on some of the plants. In N-sufficient plants over this time-scale, there was a small decline in photosynthetic rate, Rubisco protein and amino acid content, with a larger decrease in carbohydrate content. Withdrawal of N from the growing medium induced a large decrease in the rate of photosynthesis (35% reduction after 8 d under the growing conditions, with a reduction also apparent at high and low measuring CO₂), which was caused by a large decrease in the amount of Rubisco protein (62% after 8 d) and Rubisco activity. Higher amounts; of hexoses preceded the loss of photosynthetic activity and sucrose and starch accumulation. Reduction of the sourcersink ratio by shading prevented the loss of photosynthetic activity and the increase in hexoses and other carbohydrates. These data indicate that the reduction of photosynthesis that accompanies N deficiency in intact plants has the characteristics of sugar repression of photosynthesis observed in model systems, but that the accumulation of hexose prior to the decline in photosynthesis is small. The possibility that sugar repression of photosynthesis under physiological conditions depends more crucially on the C:N status of leaves than the carbohydrate status alone is discussed.     

NTAGONISM BETWEEN CADMIUM AND IRON IN THE MARINE DIATOM THALASSIOSIRA WEISSFLOGII1

Cadmium inhibits iron uptake and assimilation in the coastal diatom Thalassiosira weissflogii Grun. The effect of cadmium on short term Fe uptake fits ft competitive binding model: where (Fe³+) and (Cd²++) tire the free ferric and cadmium ion concentrations, respectively. The apparent binding constant K_cds, is calculated to be ca. lO^8.2M^-1 compared to a K^fe of lO¹⁹ M^-1. At low free ferric ion concentrations. interference of cadmium with iron transport (at pCd = 8 and pFe* < 20) results in a simultaneous decrease in growth rate and Fe accumulation to a level known 1o limit growth. Upon decreasing the free cadmium ion concentration, cells accumulate a large amount oj iron prior to resumption of normal growth. At higher free ferric ion concentrations (pFe* < 20) normal or elevated Fe quotas are absented but “luxury consumption” of iron still occurs upon reversal of toxicity. Evidence that these algae with high cellular iron quotas are effectively Fe deficient is provided by a decrease in the cytochrome f/chlorophyll a ratio and a much greater decrease in NO₃- reductase activity than in aldolase activity or H¹⁴C0₃ assimilation. Under the conditions of this study, cadmium had little effect on Si accumulation. The transport of methylamine (an analog of NH⁺₄) is unaffected by short term exposure to high free cadmium ion concentration but is greatly inhibited upon long term (97 h) exposure.     

Effects of zinc and/or iron deficiency on rectal temperature in rats

O'Dell et al. reported that rectal temperature was decreased by zinc deficiency in rats. However, it is not known whether a combined deficiency of zinc and iron affects rectal temperature. Forty 4-wk-old male Sprague-Dawley rats were assigned into four dietary treatment groups of 10 rats each for the 4-wk study: zinc-deficient group (4.5 mg Zn and 35 mg Fe/kg diet; −Zn), iron-deficient group (30 mg Zn/kg diet, no supplemental iron; −Fe), zinc/iron-deficient group (4.5 mg Zn/kg diet, no supplemental iron; −Zn−Fe), and control group (AIN-93G; Cont). At d 24–27, the rectal temperature was determined. The rectal temperature of the −Zn group was significantly lower than the Cont group. The rectal temperature of the −Zn−Fe group was similar to that of the Cont group, although thyroid-stimulating hormone and total thyroxin concentrations were the lowest in the −Zn−Fe group among all groups. The pattern of the plasma nitrate/nitrite concentrations across groups was similar to rectal temperature. Although observation of the rectal temperature is not conclusive, the balance between zinc and iron intake seems to determine the body temperature set point. These results suggest that the thermogenic effect of thyroid hormones is not throught to influence the paradoxical maintenance of rectal temperature in combined deficiency of zinc and iron.     

Security-driven scheduling for data-intensive applications on grids

Security-sensitive applications that access and generate large data sets are emerging in various areas including bioinformatics and high energy physics. Data grids provide such data-intensive applications with a large virtual storage framework with unlimited power. However, conventional scheduling algorithms for data grids are unable to meet the security needs of data-intensive applications. In this paper we address the problem of scheduling data-intensive jobs on data grids subject to security constraints. Using a security- and data-aware technique, a dynamic scheduling strategy is proposed to improve quality of security for data-intensive applications running on data grids. To incorporate security into job scheduling, we introduce a new performance metric, degree of security deficiency, to quantitatively measure quality of security provided by a data grid. Results based on a real-world trace confirm that the proposed scheduling strategy significantly improves security and performance over four existing scheduling algorithms by up to 810% and 1478%, respectively.

Magnesium deficiency in young Norway spruce (Picea abies [L.] Karst.) trees induced by NH4NO3 application

Young Norway spruce trees were grown in 94 pots (2 per pot) on soil substrate derived from granite with low Mg saturation and were fertilized with different amounts of NH₄NO₃ (in total 25, 61, and 97 kmol N ha^-1) over a period of four years, partly at an experimental station, partly at a high-elevation site in the Bavarian Forest. A fourth set of trees received 9.4 kmol Mg ha^-1 in addition to 25 kmol N. Depending on the treatment, needle chlorosis developed in the course of the experiment. Improved light conditions after three years accelerated the yellowing process. The chlorotic Norway spruce trees showed a severe Mg deficiency and an imbalanced N:Mg ratio. The shoot length increment, the stem diameter, and the needle weights however were not influenced by the fertilization. Excessive applications of NH₄NO₃caused the substrate to become depleted of Mg. The successful experimental induction of the characteristic tip yellowing of older needles of Norway spruce growing on acidic soils at higher altitudes allowed hypotheses on the causes and processes of this type of forest decline to be tested.     

Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two Arabidopsis genotypes

In a low-input agricultural context, plants facing temporalnutrient deficiencies need to be efficient. By comparing theeffects of NO-starvation in two lines of Arabidopsis thaliana (RIL282 and 432 from the Bay-0xShahdarapopulation), this study aimed to screen the physiological mechanismsallowing one genotype to withstand NO-deprivation better than another and to rate the relative importance of processessuch as nitrate uptake, storage, and recycling. These two lines,chosen because of their contrasted shoot N contents for identicalshoot biomass under N-replete conditions, underwent a 10 d nitratestarvation after 28 d of culture at 5 mM NO. It was demonstrated that line 432 coped betterwith NO-starvation, producing higher shoot and root biomass and sustaining maximal growthfor a longer time. However, both lines exhibited similar featuresunder NO-starvation conditions. In particular, the nitrate pool underwent the same drastic andearly depletion, whereas the protein pool was increased to asimilar extent. Nitrate remobilization rate was identical too.It was proportional to nitrate content in both shoots and roots,but it was higher in roots. One difference emerged: line 432had a higher nitrate content at the beginning of the starvationphase. This suggests that to overcome NO-starvation, line 432 did not directly rely on theN pool composition, nor on nitrate remobilization efficiency,but on higher nitrate storage capacities prior to NO-starvation. Moreover, the higher resistanceof 432 corresponded to a higher nitrate uptake capacity anda 2–9-fold higher expression of AtNRT1.1, AtNRT2.1, andAtNRT2.4 genes, suggesting that the corresponding nitrate transportersmay be preferentially involved under fluctuating N supply conditions. Key words: Arabidopsis thaliana, genetic variability, N partitioning, N recycling, N use efficiency, nitrate deficiency, nitrate remobilization rate, nitrate transporter gene expression, nitrogen reserves, plant development Received 12 July 2007; Revised 21 November 2007 Accepted 17 December 2007