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11.
Influenza is prevalent worldwide, and the teratogenic effects of influenza infection have been suspected to occur within the developing central nervous system. We herein report the sequelae of influenza B viral infection during early chick embryogenesis. Chick embryos at Hamburger-Hamilton stage 9 were infected by an in ovo injection under the blastoderm of influenza B virus (B/Taiwan/25/99). At 48 h after infection, gross malformations of the eye and brain, ranging from 25 to 58% of 168 infected embryos, were observed, in contrast to 3–6% among 71 mock-infected controls (p < 0.0001 for both eye and brain malformations). Histological analyses showed extensive tissue degeneration and aggregates of cells in the head mesenchyme, suggesting cell death and heterotopia. Influenza B viral RNA was directly localized by in situ hybridization with probes specific for the HA segment. Viral RNA was extensively detected in the head surface ectoderm and in the lung bud. In the developing brain, viral RNA was specifically located in the anterior neural retina, habenular area, mid-thalamus, and rhombencephalon. Our data show that influenza B virus can be a teratogenic agent in neural and nonneural embryonic tissues, raising concern for transplacental infection during early pregnancy. 相似文献
12.
The effect of copper (Cu) deficiency on the reproduction and development in Xenopus laevis was evaluated, culminating in the development of a defined concentration-response relationship. Separate groups of four adult
frog pairs were fed one of three diets for 28 d: (1) low-copper (−Cu); (2) copper supplemented (+Cu); and (3) ASTM standard
beef liver and lung (BLL). Embryos collected from frogs administered the −Cu diet had markedly decreased egg masses and viability
rates and an increased rate of necrosis when compared to the other dietary treatments. Malformations in −Cu larvae included
maldevelopment of the heart, eye, craniofacial region, brain, and notochord. Larvae from adults administered the −Cu diet
showed delayed abnormal hindlimb development, characterized as selective reductive deficiencies distal to the femur, with
poor cartilaginous development. A U-shaped dose-response curve characteristic of nutritional essentiality was developed for
Cu. Overall, these studies indicated that embryos produced from frogs administered a −Cu diet are substantially less viable
than embryos from frogs administered a +Cu or copper-adequate (BLL) diet. 相似文献
13.