模拟酸雨和铝调控对茶叶主要化学品质与铝积累的影响

为研究模拟酸雨和铝(Al)对茶叶主要化学品质与Al积累的影响及其交互作用,采用3个酸度水平(pH 3.0、4.0、5.0)和4种Al浓度水平(0、10、20、30 mg L-1),用溶液培养法研究茶叶的主要化学指标和Al含量的异同.结果表明,在模拟酸雨下,茶叶的茶多酚和咖啡碱含量随酸度增加先增加后下降,氨基酸、儿茶素和...     

贵州东部地区茶园土壤中气和前气门螨种类调查研究（蜱螨亚纲）

报道了贵州东部地区茶园土壤中气门和前气门螨类29种,分属2亚目11科。     

茶多酚对NASH 大鼠肝脏组织VEGF 及氧化应激的影响

目的:茶多酚对NASH大鼠肝脏组织VEGF及氧化应激的影响。方法:雄性SD大鼠30只,随机分为3组,正常对照组、模型组、茶多酚治疗组。正常组普通饲料喂养,模型组喂高脂饮食,茶多酚治疗组在高脂饮食12周后茶多酚(150mg(/kg.d)灌胃治疗,16周末处死各组大鼠,留取肝脏组织,观察各组大鼠肝组织病理改变,测定其肝脏丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性以及血管内皮生长因子(VEGF)、Ⅰ、Ⅲ型胶原的表达。结果:模型组大鼠肝组织中SOD活性降低而MDA含量以及VEGF、Ⅰ、Ⅲ型胶原表达均明显高于正常组。茶多酚治疗可减轻肝纤维化程度,显著升高肝组织中SOD活性、降低MDA含量以及VEGF、Ⅰ、Ⅲ型胶原表达水平。结论:茶多酚可通过抑制肝纤维化组织VEGF表达,降低肝组织氧化应激水平而发挥抗肝纤维化作用。     

The occurrence of nucleopolyhedrovirus infecting Hyposidra talaca (Geometridae: Lepidoptera), a tea defoliator from North-East India

We identified the nucleopolyhedrovirus (HytaNPV), specific to Hyposidra talaca, a major defoliator of tea in India by partial amplification of polyhedrin gene. The sequence analysis revealed HytaNPV is closely related to NPVs that infect Buzura suppressaria and Ectropis obliqua, in China.     

Modulatory Effects of Curcumin and Green Tea Extract against Experimentally Induced Pulmonary Fibrosis: A Comparison with N‐Acetyl Cysteine

The study was aimed to investigate the protective effect of green tea extract (GTE), curcumin, and N‐acetyl cysteine (NAC) on experimentally induced pulmonary fibrosis. Curcumin (200 mg/kg b.w), GTE (150 mg/kg b.w), and NAC (490 mg/kg b.w) were administered orally for 14 days with concomitant administration of cyclophosphamide (CP). Lung fibrosis was assessed by measuring hydroxyproline and elastin levels and confirmed by histopathological examination. Oxidative stress was also observed in the CP group. Lung myeloperoxidase activity was significantly decreased in animals of the CP group. N‐acetyl‐β‐d ‐glucosaminidase, leukotriene C₄, and protein were increased in bronchoalveolar lavage fluid (BALF). Transforming growth factor‐β, interleukin ‐1β, and histamine were increased in both serum and BALF. All modulators markedly attenuated the altered biochemical parameters as compared to CP‐treated rats. These results suggest the possibility of using these treatments as protective agents with chemotherapy and as protective agents for lung fibrosis. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:461‐468, 2012; View this article online at wileyonlinelibrary.com . DOI 10:1002/jbt.21447     

茶园节肢动物类群多样性和稳定性研究

对常年用人工或除草剂除草的茶园和地面种植长节耳草(Hedyotis uncinella)的茶园茶树生境节肢动物类群的多样性和稳定性进行了分析和比较．结果表明,复合茶园节肢动物类群主要特征的丰富度值除一次低于4外,其他都在4～8之间,最高达7．7403．单一园丰富度值大部分在4～6之间,最高的为6．7810;复合园丰富度值平均值为5．4672±0．3483,高于单一园相应值4．8809±0．3175．复合园节肢动物Shannon-Weiner多样性指数平均值为3．8535±0．1232,比单一园相应值3．4654±0．1856高．复合园均匀度指数平均值0．8781±0．012,高于单一园均匀度值0．8261±0．038．相反,复合园节肢动物优势集中度平均值(0．0992±0．009)低于单一园值(0．1636±0．038)．复合园稳定性指标Ss／Si和Sn／Sp比单一园的高,而复合园多样性变异系数ds／dm值(0．1107)比单一园相应值(0．1855)低．研究表明,复合茶园生态系统有比单一茶园生态系统更丰富的生物多样性和更高的系统稳定性．     

Enhancement of antibacterial effects of epigallocatechin gallate, using ascorbic acid

Although plant polyphenols such as (−)-epigallocatechin gallate (EGCG) have antibacterial activity towards methicillin-resistant Staphylococcus aureus (MRSA), such polyphenols are unstable in solution. Because the instability of polyphenols is attributable to their oxidation, we examined the effects of antioxidants and inhibitors of polyphenol oxidation on the maintenance of polyphenol antibacterial activity. The antibacterial activity of EGCG was enhanced in the presence of ascorbic acid, and ascorbic acid was the most effective for retaining the concentration of stable EGCG. On the other hand, the antibacterial activity of EGCG was lowered in the presence of casein in spite of its suppressing effect on the EGCG decrease. The effect of EGCG on the antibiotic resistance of MRSA was also enhanced in the presence of ascorbic acid. The addition of an antioxidant may affect other pharmacological effects of polyphenols in analogous ways, although this does not mean the clinical usefulness of the addition directly.     

茶多酚对酒精性肝病大鼠核因子κB及环氧合酶2表达的影响

目的观察茶多酚对大鼠酒精性肝病的干预作用及其对核因子kB（NF-kB）和环氧合酶2（COX-2）表达的影响。方法32只Wistar大鼠,随机分为3组,对照组（6只）用玉米油;模型组（13只）用白酒＋玉米油;茶多酚组（13只）在模型组基础上给予茶多酚。7周后处死大鼠,检测血清中天门冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和丙二醛（MDA）含量。光镜和电镜观察肝组织细胞及亚细胞结构的改变;逆转录聚合酶链反应（RT-PCR）测肝组织中NF-kB及COX-2的mRNA表达;免疫组织化学法检测肝组织中NF-kB和COX-2蛋白的表达。结果模型组肝细胞损伤明显,茶多酚组较轻。模型组大鼠血清ALT较对照组明显升高（P〈0．01）;与模型组相比,茶多酚组大鼠血清ALT、AST、MDA明显降低（P〈0．01）。RT-PCR及免疫组化均显示,肝组织中NF-kB及COX-2表达模型组较对照组明显升高（P〈0．01）,茶多酚组较模型组明显降低（P〈0．01）。结论茶多酚对大鼠酒精性肝损伤有保护作用,可能是通过抑制NF-kB的活化和COX-2的表达来实现的。     

Cycling treatment of anaerobic and aerobic incubation increases the content of γ-aminobutyric acid in tea shoots

Summary. γ-Aminobutyric acid (GABA), a hypotensive compound, is formed from glutamic acid under anaerobic condition in tea shoots. Glutamic acid was exhausted in the first three hours of anaerobic incubation and the increase of GABA stopped. After that, when tea shoots were released under aerobic condition, glutamic acid reproduced rapidly. After one hour of aerobic incubation, tea shoots were given three hours of anaerobic incubation again and then accumulated glutamic acid changed to GABA. The content of GABA increased much more than usual anaerobic incubation. GABA was more in the tea stem than in the leaf. Received January 4, 2000 Accepted March 1, 2000     

Monosaccharide compositions of Danaus plexippus (monarch butterfly) and Trichoplusia ni (cabbage looper) egg glycoproteins

Monosaccharide compositions of eggs from Danaus plexippus (monarch butterfly) and Trichoplusia ni (cabbage looper) were analyzed. Analyses were performed mainly with high performance anion exchange chromatography (HPAEC) using crude extracts of eggs or SDS-PAGE separated and PVDF-blotted protein bands. Man and GlcN were the major components in all cases, but low levels of Gal and Fuc were possibly present in some samples. Some T. ni egg glycoproteins even contained GalN. Although a peak comigrating with Neu5Ac could be detected with HPAEC-PAD or RP-HPLC (fluorometry) after derivatization with 1,2-diamino-4,5-methylenedioxy-benzene, the quantities were too small to be significant as an integral part of the analyzed glycoproteins. These data suggests that most of glycans on the glycoproteins are pauci-Man type N-glycans, but a small portion of N-glycan may be either hybrid type or complex type.