黄精凝集素Ⅱ分子稳定性与生物学活性研究

黄精凝集素Ⅱ分子稳定性与生物学活性研究鲍锦库,曾仲奎,周红（四川大学生物系,成都,６１００６４）本文在黄精凝集素Ⅱ纯化及性质研究的基础上,应用多种变性条件,研究其分子特性,同时对分子的巯基和色氨酸进行修饰,研究该凝集素分子保持其生物学活性与这些基团的...     

Studying dynamic process of photosensitization by ESR method

By kinetic methods, functional relation of TAN radical, produced in the process of TEMPONE trapping O2, vs. time during photosensitization was established. Accordingly relative rate constants of generating all kinds of active intermediates through types I and II mechanism of photosensitization can be calculated. Using the formula and experimental results, the relative rate constants of generating O2, O2 and PS2 of three kinds of perylenequinone photosensitizer: HA, HB and CP in DMF-H20 and DMSO-H2O system were calculated, and then the structure-activity relationship of perylenequinone photosensitizer and the relation between photosensitivity and solvent was studied.     

New red flower germplasm lines of cotton selected from hybrid of Gossypium hirsutum × G. bickii

By means of dropping GA3 (50 ppm) and NAA (40 ppm) on the hybrid boll-embryo culture in vitro, one F1 plant of G. hirsutum G. bickii was obtained; when F1 branches were grafted on upland cotton and then back-crossed with upland cotton under short-day and cooler-night condition, some BC1 seeds could be harvested. The characteristic segregation was very violent in early generation. Through 3 times of back-crossing and selecting, ten stable hybrid lines with the character of both male parent (viz. red petal-purple spot and strong fibre) and female par-ent (plant type, earliness, white fibre, lint length, etc. ) were established. These lines were assigned as HB red flow-er lines (HBRL). Transference of character of G. bickii to upland cotton was proved to be successful for the first time. These new germplasms may play an important role in both the genetic research and new cotton variety breeding.     

蛋白电泳铜染色后胶中生物活性IL-2的回收

SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)已广泛用于蛋白质样品组分分析。通常的方法染色后蛋白质和肽即固定在凝胶上,尽管凝胶用SDS平衡后可对蛋白质进行洗脱或转移,但由于回收率低、耗时,同时回收的蛋白只能保存其抗原性而生物活性丧失,因而限制了SDS-PAGE在蛋白质回收领域中的应用。最近     

影响Klebsiella oxytoca固氮酶和氢酶活性的生理条件

联合固氮菌在自然界广泛存在,它在提供土壤和作物氮源上起着一定的作用。近年来发现Klebsiella oxytoca参与水稻联合共生体系,但对该菌固氮生理特征的研究则相当缺乏。作为资料性的工作,本文对K.oxytoca NG13固氮酶和氢酶活性表达的生理条件作了分析,为应用研究以及深入进     

Bioinspired Closed-loop CPG-based Control of a Robot Fish for Obstacle Avoidance and Direction Tracking

This paper presents a study on bioinspired closed-loop Central Pattern Generator(CPG)based control of a robot fish for obstacle avoidance and direction tracking.The biomimetic robot fish is made of a rigid head with a pair of pectoral fins,a wire-driven active body covered with soft skin,and a compliant tail.The CPG model consists of four input parameters:the flapping amplitude,the flapping angular velocity,the flapping offset,and the time ratio between the beat phase and the restore phase in flapping.The robot fish is equipped with three infrared sensors mounted on the left,front and right of the robot fish,as well as an inertial measurement unit,from which the surrounding obstacles and moving direction can be sensed.Based on these sensor signals,the closed-loop CPG-based control can drive the robot fish to avoid obstacles and to track designated directions.Four sets of experiments are presented,including avoiding a static obstacle,avoiding a moving obstacle,tracking a designated direction and tracking a designated direction with an obstacle in the path.The experiment results indicated that the presented control strategy worked well and the robot fish can accomplish the obstacle avoidance and direction tracking effectively.     

英国的侧盘菌属:属的概况及大型孢子种的研究(英文)

对侧盘菌属在英国的研究概况进行了评述,研究侧重于Otidea apophysata和O.platyspora两个具有大型子囊孢子的种。同时,对4个错误地用于大型孢子种的名称进行了订正:O. abietina(Pseudotis属的模式种)是含糊名称(nomen ambiguum);O.cochleata也为含糊名称;O. felina是O.alutacea的同物异名,并为后者指定了选模式;将O.umbrina处理为O.bufonia的同物异名。此外,确定了Otidea violacea的分类地位。     

不同产地生漆中漆酚清除自由基及构效关系的初步研究

为更好地开发和利用生漆资源,探寻生漆中主要有效成分漆酚的抗氧化性能,为生漆的深加工提供理论依据和参数。分别应用丙酮提取中国和越南生漆,对得到的漆酚粗提物中的漆酚含量进行测定,建立了阳性对照组Vc和二丁基羟基甲苯(BHT)基础的DPPH、ABTS及O2-.法,对提取物抗氧化性能进行测试;在此基础上,结合紫外光谱分析了中国和越南生漆中漆酚含量及结构差异性,并初步分析其抗氧化的构效关系。结果显示,各地生漆中漆酚提取物具有强的抗氧化活性:中国毛坝生漆提取物抗氧化活性最强,其次是越南红漆和中国小木漆;紫外光谱分析亦表明毛坝生漆中含有丰富的不饱和键可能与其较强的抗氧化性相关。     

介绍一种改良的伊红染色液

在常规HE染色过程中,要求切片核浆对比要分明,细胞浆染色质量的好坏亦至关重要。伊红是细胞浆常用的染色剂,其配制方法较多,多用水溶性伊红染色液,在染色过程中发现水溶性伊红染色液易出现着色过淡、核浆共染及染色后的切片置一段时间后易褪色等现象,给观察切片的组织形态带来一定困难。我们根据伊红的化学性质和染色理论,结合实际应用情况,对传统伊红染液的配制方法进行了一些改良,收到了理想的染色效果。     

An Efficient Rice Mutagenesis System Based on Suspension-Cultured Cells

Plant mutants are important bio-resources for crop breeding and gene functional studies. Conventional methods for generating mutant libraries by mutagenesis of seeds with physical or chemical agents are of low efficiency. Here, we developed a highly-efficient ethyl methanesulfonate (EMS) mutagenesis system based on suspension-cultured cells, with rice (Oryza sativa L.) as an example. We show that treatment of suspension-cultured tiny cell clusters with 0.4% EMS for 18-22h followed by differentiation and regeneration produced as high as 29.4% independent mutant lines with visible phenotypic variations, including a number of important agronomic traits such as grain size, panicle size, grain or panicle shape, tiller number and angle, heading date, male sterility, and disease sensitivity. No mosaic mutant was observed in the mutant lines tested. In this mutant library, we obtained a mutant with an abnormally elongated uppermost internode. Sequencing and functional analysis revealed that this is a new allelic mutant of eui (elongated uppermost internode) caused by two point mutations in the first exon of the EUI gene, representing a successful example of this mutagenesis system.