排序方式: 共有71条查询结果,搜索用时 0 毫秒
51.
《Cell Stem Cell》2019,24(5):736-752.e12
- Download : Download high-res image (236KB)
- Download : Download full-size image
52.
53.
The last decade has radically renewed our understanding of higher order chromatin folding in the eukaryotic nucleus. As a result, most current models are in support of a mostly hierarchical and relatively stable folding of chromosomes dividing chromosomal territories into A‐ (active) and B‐ (inactive) compartments, which are then further partitioned into topologically associating domains (TADs), each of which is made up from multiple loops stabilized mainly by the CTCF and cohesin chromatin‐binding complexes. Nonetheless, the structure‐to‐function relationship of eukaryotic genomes is still not well understood. Here, we focus on recent work highlighting the biophysical and regulatory forces that contribute to the spatial organization of genomes, and we propose that the various conformations that chromatin assumes are not so much the result of a linear hierarchy, but rather of both converging and conflicting dynamic forces that act on it. 相似文献
54.
55.
56.
57.
58.
59.
肾缺血再灌流损伤过程中肾小管上皮细胞色素氧化酶活性变化及TAD防治作用的研究 总被引:1,自引:0,他引:1
本实验选用大鼠一侧肾切除,对侧肾缺血60min动物模型,用组织化学方法观察再灌流15min,24h髓质外带肾小管上皮细胞色素氧化酶活性的变化及还原型谷胱甘肽(TAD)对它们的影响。结果发现60min肾缺血后再灌流可致细胞色素氧化酶活性呈进行性降低。给TAD能一定程度地保护细胞色素氧化酶活性。提示氧自由基可能损害细胞色素氧化酶活性,细胞色素氧化酶活性降低在肾缺血再灌流损伤中可能起重要作用。 相似文献
60.
In eukaryotes, the genome is hierarchically packed inside the nucleus, which facilitates physical contact between cis-regulatory elements (CREs), such as enhancers and promoters. Accumulating evidence highlights the critical role of higher-order chromatin structure in precise regulation of spatiotemporal gene expression under diverse biological contexts including lineage commitment and cell activation by external stimulus. Genomics and imaging-based technologies, such as Hi-C and DNA fluorescence in situ hybridization (FISH), have revealed the key principles of genome folding, while newly developed tools focus on improvement in resolution, throughput and modality at single-cell and population levels, and challenge the knowledge obtained through conventional approaches. In this review, we discuss recent advances in our understanding of principles of higher-order chromosome conformation and technologies to investigate 4D chromatin interactions. 相似文献