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971.
Giovanna Riccardi Edda De Rossi Giuliano Della Valle Orio Ciferri 《Plant molecular biology》1985,4(2-3):133-136
An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. 相似文献
972.
Atsushi Kato Yoichi Iida Kimitaka Yakura Shigeyuki Tanifuji 《Plant molecular biology》1985,5(1):41-53
Cleavage of Vicia faba nuclear DNA with the restriction endonuclease BamHI yielded discrete size classes of 250, 850, 900, 990, 1 150, 1 500 and 1 750 bp of highly repetitive DNA. Each of these sequence families comprised about 3% of the total genomic DNA. Some sequence members from each sequence family were cloned in pBR322 and their primary structures determined. Computer analyses of nucleotide sequences suggested the existence of about 60 bp sequence periodicity within the repeating unit of the 990 bp sequence family, though the extent of homology among the surmised shorter subrepeat units was very low. With other BamHI sequence families, however, the data did not show any clear internal sequence periodicity. The repeat units of the 850 bp and 1 750 bp sequence families contained nucleotide sequences homologous to the 250 bp family sequence. No sequence relationship between or among other sequence families was observed. There was 13–25% sequence variation among 6 cloned members of the 250 bp family and probably also among those of other BamHI repeat families. DNA sequences homologous to these V. faba BamHI repeat families were detected in Pisum sativum DNA by Southern blot hybridization. Furthermore, very weak cross-hybridization was observed with plant DNAs from Phaseolus vulgaris, Triticum aestivum, Cucumis sativus and Trillium kamtschaticum. 相似文献
973.
用“剪切重组”术及内引物法测定了克隆在M_(13)mp_8载体中的HBVDNA 1.3Kb片段的完整顺序,简化了操作过程,为连续测定大段DNA的顺序提供了简单、快速、有效的途径。 相似文献
974.
The cloning vector pME290 (6.8 kb), which is derived from thePseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1–3 copies) and pME294 (about 15–20 copies) were isolated. These vectors were used in the characterization of theP. aeruginosa argF gene encoding ornithine carbamoyltransferase. 相似文献
975.
Sequence of Two mRNAs Encoding Active Rat Tryptophan Hydroxylase 总被引:16,自引:9,他引:7
Michèle C. Darmon Bernard Guibert Vincent Leviel Mireille Ehret Michel Maitre Jacques Mallet 《Journal of neurochemistry》1988,51(1):312-316
Two full-length cDNA clones that encode functional rat tryptophan hydroxylase (EC 1.14.16.4), the key enzyme in serotonin synthesis, have been isolated from a rat pineal gland library. These two clones correspond to the 1.8- and 4-kilobase mRNA species, respectively. They contain the same coding sequence corresponding to a 51,010-dalton protein and differ in the length of their 3' untranslated regions. 相似文献
976.
犬Ⅰ型腺病毒DNA的酶切分析及分子克隆 总被引:2,自引:1,他引:1
犬Ⅰ型腺病毒(CAV-1)弱毒用限制性内切酶EcoR Ⅰ,BamH Ⅰ,Pst Ⅰ,Sph Ⅰ和Hind Ⅲ消化分析后其图谱与强毒株相比没有差异。将弱毒DNA用Pst Ⅰ完全消化后以鸟枪法克隆到载体质粒pBluescrip'SK中,经用光生物素标记的CAV-1 DNA杂交筛选以及Pst Ⅰ分析重组质粒证明已将分子量为5.5,3.5,2.85,1.2,0.32和0.28Kb的CAV-1DNA片段克隆到质粒中。克隆到的这些片段将可考虑进一步研究作为探针检测犬及狐狸等野生动物的腺病毒感染。 相似文献
977.
用DNA合成仪合成寡聚脱氧核苷酸。用T4-DNA连接酶把这些寡聚脱氧核苷酸重组成双链DNA。这两个双链DNA的上游是T7-启动子,下游分别编码酵母丙氨酸tRNA的5′半分子(1-35位核苷酸)和3′半分子(35-76位核苷酸)。再把这两个双链DNA克隆到PUC 12质粒中。经点杂交筛选和DNA顺序测定证明克隆是成功的。 相似文献
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979.
980.