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961.
962.
Yuma Okamoto Yoshitaka Nagai Nobuhiro Fujikake H. Akiko Popiel Tohru Yoshioka Takashi Inui 《Biochemical and biophysical research communications》2009,378(3):634-639
Proteins with an abnormally expanded polyglutamine (polyQ) stretch are prone to change their conformations, leading to their aggregation, and cause inherited neurodegenerative diseases called the polyQ diseases. Although screening for polyQ aggregation inhibitors has been extensively performed, many common false-positive hits have been identified so far. In this study, we employed surface plasmon resonance (SPR) to characterize the binding specificities and affinities of polyQ aggregation inhibitors to the expanded polyQ stretch. SPR successfully detected specific binding of polyQ binding peptide 1 (QBP1) to the expanded polyQ stretch (Kd = 5.7 μM), and non-specific binding of Congo red to polyQ proteins independent of their polyQ-length. Binding affinities of polyQ aggregation inhibitors to the expanded polyQ stretch were correlated with their inhibitory effects on polyQ aggregation. We therefore conclude that SPR is a useful technique for screening for specific polyQ aggregation inhibitors as promising therapeutic candidates for the currently untreatable polyQ diseases. 相似文献
963.
Yu-Ching Lin Iren Wang Jiahn-Haur Liao Chinpan Chen 《Biochemical and biophysical research communications》2009,388(1):62-313
Lon protease has been well studied in many aspects; however, the DNA-binding specificity of Lon in prokaryotes has not been clearly identified. Here we examined the DNA-binding activity of Lon protease α-domains from Brevibacillus thermoruber (Bt), Bacillus subtilis (Bs), and Escherichia coli (Ec). MALDI-TOF mass spectroscopy showed that the α-domain from Bt-Lon binds to the duplex nucleotide sequence 5′-CTGTTAGCGGGC-3′ (ms1) and protected it from DNase I digestion. Surface plasmon resonance showed that the Bt-Lon α-domain binds with ms1 double-stranded DNA tighter than Bs- and Ec-Lon α-domains, whereas the Bt-Lon α-domain has dramatically lower affinity for double-stranded DNA with 0 and 50% identity to the ms1 binding sequence. Our results indicated that Bt-Lon α-domain plays a critical role with ms1 sequence in the DNA-binding specificity. 相似文献
964.
Size- and shape-controlled syntheses of silver and gold nanoparticles have been successfully developed using partially hydrolyzed starch vermicelli templates as green nanoreactors for the growth of nanoparticles. Mung bean vermicelli is of interest due to the higher amylose content and its transparency, allowing the formation of coloured particles on the vermicelli to be observed. The as-prepared silver and gold nanoparticles were characterized by UV–Visible spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction (XRD). The carbonization of as-prepared vermicelli at 200 °C, 300 °C, and 500 °C was carried out to investigate nanoparticles embedded in the starch vermicelli templates. TEM of carbonized samples revealed the interesting patterns of gold nanorods and silver nanowire-liked assemblies along with carbon nanotubes. The carbonization of silver nanoparticles at 500 °C resulted to the loss of starch vermicelli capping nanoparticles and this led to the higher diffusion rate of nanoparticles to generate silver nanodendrites on TEM images. XRD data of carbonized yellow and purple silver nanoparticles revealed the presence of silver nanoparticles and a mixture of silver and silver chloride nanoparticles, respectively. This approach offers a great potential to design new fine structures of vermicelli and utilize its structure as a template for the large-scale synthesis of size- and shape-controlled silver and gold nanoparticles for chemical and biological applications. 相似文献
965.
Li-Cheng Song Bin Gai Hu-Ting Wang Qing-Mei Hu 《Journal of inorganic biochemistry》2009,103(5):805-812
As an extension of our study on the H-cluster model compounds, a series of diiron propanediselenolate (PDS)-type models have been successfully synthesized. Reaction of diselenol HSe(CH2)3SeH with Fe3(CO)12 in THF (tetrahydrofuran) at reflux gave the parent model compound [μ-Se(CH2)3Se-μ]Fe2(CO)6 (1) in 48% yield. Further reaction of 1 with PPh3 or PPh2H in the presence of Me3NO in MeCN at room temperature afforded the phosphine-monosubstituted model compounds [μ-Se(CH2)3Se-μ]Fe2(CO)5(L) (2, L = PPh3; 3, L = PPh2H) in 76% and 68% yields, respectively. Similarly, the N-heterocyclic carbene IMes-monosubstituted model compound [μ-Se(CH2)3Se-μ]Fe2(CO)5(IMes) (4) could be prepared in 46% yield by reaction of imidazolium salt IMes · HCl with n-BuLi followed by treatment of the resulting IMes ligand with 1 in THF at room temperature. Compounds 1-4 were fully characterized by elemental analysis and various spectroscopic methods. While the structures of 1-4 were further confirmed by X-ray crystallography, the comparative study of 1 and its analog [μ-S(CH2)3S-μ]Fe2(CO)6 demonstrates that 1 is a better catalyst for TsOH proton reduction to hydrogen under electrochemical conditions. 相似文献
966.
Katsumune Takahashi Rumi Shizume Katsumi Uchida Hirofumi Yajima 《Journal of Biorheology》2009,23(1):64-71
Single-walled carbon nanotubes are novel molecular-scale wires having excellent anti-adhesion properties with regard to platelets.
On the other hand, chitosan is a partially de-acetylated derivative of chitin that has a critical role in cell attachment
and growth. The aim of this study was to investigate how carbon nanotubes improve the blood biocompatibility of chitosan film.
We prepared composite films with various concentrations of chitosan/carbon nanotubes (CS/CNTs) (1.3–6.3 wt%). The sample surfaces
were characterized by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and contact
angle measurements. The surface characterization revealed that the surface of the CS/CNTs composite film became more hydrophobic
with increasing amounts of CNTs. Cell attachment tests using bovine aortic endothelial cells (BAECs) indicated that CS/CNTs
composite films retained their cell adhesion ability. The blood compatibility of the CS/CNTs composite films was evaluated
using the blood platelet adhesion and activation tests in vitro. Platelet adhesion results confirmed that platelet adhesion
and the formation of a platelet network were inhibited on composite films with higher concentrations of CNTs (5.1 wt%). Our
experimental results show that the novel composite film containing CS/CNTs possesses two paradoxical characteristics, namely,
good adherence of endothelial cells and minimum adherence and activation of platelets, making this film a promising antithrombogenic
material for use in the biomedical field. 相似文献
967.
Sarkar M.A. Kawsar Tomoharu Takeuchi Ken-ichi Kasai Yuki Fujii Ryo Matsumoto Hidetaro Yasumitsu Yasuhiro Ozeki 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2009,152(4):382-389
A lectin recognizing D-galactose was purified from the pacific annelid Perinereis nuntia ver. vallata (Polychaeta) by affinity chromatography. Hemagglutinating activity, with a very low titer suggesting the presence of lectin appeared in the supernatant from the homogenization of body with Tris-buffered saline. However, dialyzed supernatant from the precipitate homogenized by galactose in the buffer revealed strong hemagglutinating activity against human erythrocytes. The crude supernatant was applied onto lactosyl–agarose column, and only the supernatant eluted from precipitate with galactose was obtained a galactose-binding lectin with 32 kDa polypeptide was obtained from the supernatant of the precipitate, extracted in presence of galactose. It suggests that the lectin tightly binds with glycoconjugate as endogenous ligand(s) in the tissue. Hemagglutinating activity against trypsinized and glutaraldehyde-fixed human erythrocytes was specifically inhibited by D-galactose, N-acetyl-D-galactosamine, lactose, melibiose, and asialofetuin. Glycan-binding profile of the lectin analyzed by frontal affinity chromatography shows that the lectin recognizes branched complex type N-linked oligosaccharides and both type 1 (Galβ1-3GlcNAc) and type 2 (Galβ1-4GlcNAc) lactosamine. The surface plasmon resonance study of the lectin against asialofetuin showed the kass and kdiss values are 5.14 × 104 M− 1 s− 1 and 2.9 × 10−3 s− 1, respectively. The partial primary structure of the lectin reveals 182 amino acids with novel sequence. 相似文献
968.
KATRI KOSTIAINEN SEIJA KAAKINEN PEKKA SARANPÄĆ BJARNI D. SIGURDSSON‡ SVEN-OLOF LUNDQVIST§ SUNE LINDER¶ ELINA VAPAAVUORI 《Global Change Biology》2009,15(2):368-379
The objective of the study was to investigate the interactive effects of elevated atmospheric carbon dioxide concentration, [CO2 ], and temperature on the wood properties of mature field-grown Norway spruce ( Picea abies (L.) Karst.) trees. Material for the study was obtained from an experiment in Flakaliden, northern Sweden, where trees were grown for 3 years in whole-tree chambers at ambient (365 μmol mol−1 ) or elevated [CO2 ] (700 μmol mol−1 ) and ambient or elevated air temperature (ambient +5.6 °C in winter and ambient +2.8 °C in summer). Elevated temperature affected both wood chemical composition and structure, but had no effect on stem radial growth. Elevated temperature decreased the concentrations of acetone-soluble extractives and soluble sugars, while mean and earlywood (EW) cell wall thickness and wood density were increased. Elevated [CO2 ] had no effect on stem wood chemistry or radial growth. In wood structure, elevated [CO2 ] decreased EW cell wall thickness and increased tracheid radial diameter in latewood (LW). Some significant interactions between elevated [CO2 ] and temperature were found in the anatomical and physical properties of stem wood (e.g. microfibril angle, and LW cell wall thickness and density). Our results show that the wood material properties of mature Norway spruce were altered under exposure to elevated [CO2 ] and temperature, although stem radial growth was not affected by the treatments. 相似文献
969.
Several skeletal muscles can be divided into sub-modules, called neuromuscular compartments (NMCs), which are thought to be controlled independently and to have distinct biomechanical functions. We looked for distinct muscle activation patterns in the triceps surae muscle (TS) using surface electromyography (EMG) during voluntary contraction. Nine subjects performed isometric and isotonic plantar flexions combined with forces along pre-defined directions. Besides the forces under the ball of the foot, multi-channel surface EMG was measured with electrodes homogeneously distributed over the entire TS. Using principal component analysis, common (global) components were omitted from the EMG signals, thereby estimating muscle activity sufficiently accurate to track fine fluctuations of force during an isotonic contraction (r = 0.80 ± 0.09). A subsequent cluster analysis showed a topographical organization of co-activated parts of the muscle that was different between subjects. Low and negative correlations between the EMG activity within clusters were found, indicating a substantial heterogeneity of TS activation. The correlations between cluster time series and forces at the foot in specific directions differed substantially between clusters, showing that the differentially activated parts of the TS had specific biomechanical functions. 相似文献
970.
Marina Cretich Dr. Gabriele Di Carlo Cinzia Giudici Sven Pokoj Iris Lauer Stephan Scheurer Marcella Chiari 《Proteomics》2009,9(8):2098-2107
Allergen microarrays are under development for a component‐resolved diagnosis of Type I (IgE‐mediated) allergies. Here we report an improved microarray coupled to microfluidics for the detection of allergen specific immunoglobulin E (IgE). The signal intensity for IgE detection in serum has been improved by using glass slides coated with a novel poly[DMA‐co‐NAS] brush copolymer which is able to immobilize allergens in their native conformation and by carrying out the incubation step in dynamic conditions. The assay, fully automated, was performed in a microcell, using a software‐controlled fluidic processor, to bring assay reagents on the surface of the array. Microfluidics turns the binding between serum immunoglobulins and immobilized allergens from a diffusion‐limited to a kinetic‐limited process by ensuring an efficient mixing of serum samples on the surface of the microarray. As a result of this, the binding of high affinity IgE antibodies is enhanced whereas that of low affinity IgG antibodies, which are present at higher concentration, is impaired paving the way to more accurate and sensitive results. 相似文献