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31.
The fluence-rate and time dependence for photoaccumulation and photodispersal ofEuglena gracilis was measured for the wild-type strain and three white mutants. For wavelengths of 453 or 463 nm the threshold for photoaccumulation
was close to 6×10−2Wm−2. Photoaccumulation increased steadily with time and reached a maximum after about 4 hr. Red light elicited substantial photoaccumulation
in the wild type and photodispersal in the white, non-photosynthetic mutant 1224-5/9f. The chromophore mediating the red-light
response needs to be a non-photosynthetic pigment which remains presently unidentified.
A whiteEuglena mutant, FB, which had retained a reduced stigma and a paraflagellar body, showed weak photoaccumulation. Two white mutants,
1224-5/1f and 1224-5/9f, both of which lacked the stigma and positive phototaxis, displayed during the first 90 min of irradiation
photodispersal; after longer irradiations they showed instead photoaccumulation. These results contradict a widely held belief
that the presence of a stigma represents a stringent requirement for photoaccumulation. Our results imply that phototaxis
is not a prerequisite for photoaccumulation.
Exogenous flavins and 5,10-methenyl-tetrahydrofolate (MTHF) influenced in a wavelength-dependent manner photoaccumulation
and photodispersal. In the wild type FAD and riboflavin (RB) caused at 453 nm an increase of the responsiveness for photoaccumulation.
The photoaccumulation of the white mutant FB, was sensitized by FMN and FAD. In the white mutant 1224-5/9f exogenous flavins
lowered the threshold for photodispersal. FMN, which absorbs only blue light, altered also the responsiveness to red light:
in the wild type FMN reduced photoaccumulation and in the white mutant 1224-5/9f it reduced photodispersal. 相似文献
32.
Geiger DL 《Journal of molecular evolution》2002,54(2):191-199
Most coding strategies that address the problem of questionable alignment (elision, case sensitive, missing, polymorphic,
gaps as presence/absence matrix) conflict with phylogenetic principles, particularly those relating to the concept of homology
(shared similiarity explained by common ancestry). In some cases, the test of conjunction is failed. In other cases, characters
that are coded ambiguously can lead to character-state optimization in the terminal taxa that conflicts with the original
observations. Only data exclusion and contraction avoid these pitfalls. In highly dissimilar sequences additional character
states can represent the available information. Two new methods that accomplish this—block and stretch coding—are introduced
here. These two new coding strategies are not in conflict with the test of conjunction and do not contradict the original
observations. They are comparable to coding practices with morphological data once the intrinsic differences due to character-state
identity and topographical identity have been taken into account. It is suggested that, of the three recoding methods, the
one is selected that preserves the maximum potential phylogenetic information as measured with the minimum number of steps
required for the particular part of the data matrix.
Received: 1 August 2000 / Accepted: 10 July 2001 相似文献
33.
Insect flight muscles have been classified as either synchronous or asynchronous based on the coupling between excitation and contraction. In the moth Manduca sexta, the flight muscles are synchronous and do not display stretch activation, which is a property of asynchronous muscles. We annotated the M. sexta genes encoding the major myofibrillar proteins and analyzed their isoform pattern and expression. Comparison with the homologous genes in Drosophila melanogaster indicates both difference and similarities. For proteins such as myosin heavy chain, tropomyosin, and troponin I the availability and number of potential variants generated by alternative spicing is mostly conserved between the two insects. The exon usage associated with flight muscles indicates that some exon sets are similarly used in the two insects, whereas others diverge. For actin the number of individual genes is different and there is no evidence for a flight muscle specific isoform. In contrast for troponin C, the number of genes is similar, as well as the isoform composition in flight muscles despite the different calcium regulation. Both troponin I and tropomyosin can include COOH-terminal hydrophobic extensions similar to tropomyosinH and troponinH found in D. melanogaster and the honeybee respectively. 相似文献
34.
Heise RL Stober V Cheluvaraju C Hollingsworth JW Garantziotis S 《The Journal of biological chemistry》2011,286(20):17435-17444
Epithelial injury is a central event in the pathogenesis of many inflammatory and fibrotic lung diseases like acute respiratory distress syndrome, pulmonary fibrosis, and iatrogenic lung injury. Mechanical stress is an often underappreciated contributor to lung epithelial injury. Following injury, differentiated epithelia can assume a myofibroblast phenotype in a process termed epithelial to mesenchymal transition (EMT), which contributes to aberrant wound healing and fibrosis. We demonstrate that cyclic mechanical stretch induces EMT in alveolar type II epithelial cells, associated with increased expression of low molecular mass hyaluronan (sHA). We show that sHA is sufficient for induction of EMT in statically cultured alveolar type II epithelial cells and necessary for EMT during cell stretch. Furthermore, stretch-induced EMT requires the innate immune adaptor molecule MyD88. We examined the Wnt/β-catenin pathway, which is known to mediate EMT. The Wnt target gene Wnt-inducible signaling protein 1 (wisp-1) is significantly up-regulated in stretched cells in hyaluronan- and MyD88-dependent fashion, and blockade of WISP-1 prevents EMT in stretched cells. In conclusion, we show for the first time that innate immunity transduces mechanical stress responses through the matrix component hyaluronan, and activation of the Wnt/β-catenin pathway. 相似文献
35.
Molecular basis of the mammalian pressure-sensitive ion channels: focus on vascular mechanotransduction 总被引:2,自引:1,他引:1
Folgering JH Sharif-Naeini R Dedman A Patel A Delmas P Honoré E 《Progress in biophysics and molecular biology》2008,97(2-3):180-195
Mechano-gated ion channels are implicated in a variety of neurosensory functions ranging from touch sensitivity to hearing. In the heart, rhythm disturbance subsequent to mechanical effects is also associated with the activation of stretch-sensitive ion channels. Arterial autoregulation in response to hemodynamic stimuli, a vital process required for protection against hypertension-induced injury, is similarly dependent on the activity of force-sensitive ion channels. Seminal work in prokaryotes and invertebrates, including the nematode Caenorhabditis elegans and the fruit fly drosophila, greatly helped to identify the molecular basis of volume regulation, hearing and touch sensitivity. In mammals, more recent findings have indicated that members of several structural family of ion channels, namely the transient receptor potential (TRP) channels, the amiloride-sensitive ENaC/ASIC channels and the potassium channels K2P and Kir are involved in cellular mechanotransduction. In the present review, we will focus on the molecular and functional properties of these channel subunits and will emphasize on their role in the pressure-dependent arterial myogenic constriction and the flow-mediated vasodilation. 相似文献
36.
H.?BleckmannEmail author H.?Schmitz G.?von der?Emde 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2004,190(12):971-981
Nature has developed a stunning diversity of sensory systems. Humans and many animals mainly rely on visual information. In addition, they may use acoustic, olfactory, and tactile cues for object detection and spatial orientation. Beyond these sensory systems a large variety of highly specialized sensors have evolved. For instance, some buprestid beetles use infrared organs for the detection of forest fires. The infrared sensors of boid and crotalid snakes are used for prey detection at night. For object detection and spatial orientation many species of nocturnal fish employ active electrolocation. This review describes certain aspects of the detection and processing of infrared and electrosensory information. We show that the study of natural exotic sensory systems can lead to discoveries that are useful for the construction of technical sensors and artificial control systems. Comparative studies of animal sensory systems have the power to uncover at least a small fraction of the gigantic untapped reservoir of natural solutions for perceptive problems. 相似文献
37.
The Catharanthus roseus Receptor‐Like Kinase 1‐like (CrRLK1L) family of 17 receptor‐like kinases (RLKs) has been implicated in a variety of signaling pathways in Arabidopsis, ranging from pollen tube (PT) reception and tip growth to hormonal responses. The extracellular domains of these RLKs have malectin‐like domains predicted to bind carbohydrate moieties. Domain swap analysis showed that the extracellular domains of the three members analyzed (FER, ANX1, HERK1) are not interchangeable, suggesting distinct upstream components, such as ligands and/or co‐factors. In contrast, their intercellular domains are functionally equivalent for PT reception, indicating that they have common downstream targets in their signaling pathways. The kinase domain is necessary for FER function, but kinase activity itself is not, indicating that other kinases may be involved in signal transduction during PT reception. 相似文献
38.
Mechanically Activated Currents in Chick Heart Cells 总被引:7,自引:0,他引:7
As predicted from stretch-induced changes of rate and rhythm in the heart, acutely isolated embryonic chick heart cells exhibit
whole-cell mechanosensitive currents. These currents were evoked by pressing on cells with a fire polished micropipette and
measured through a perforated patch using a second pipette. The currents were carried by Na+ and K+ but not Cl−, and were independent of external Ca2+. The currents had linear I/V curves reversing at −16 mV and were completely blocked by Gd3+≥ 30 μm and Grammostola spatulata venom at a dilution of 1:1000. Approximately 20% of cells showed time dependent inactivation. In contrast to direct mechanical
stimulation, hypotonic volume stress produced an increase in conductance for anions rather than cations—the two stimuli are
not equivalent. The cells had two types of stretch-activated ion channels (SACs): a 21 pS nonspecific cation-selective reversing
at −2 mV and a 90 pS K+ selective reversing at −70 mV in normal saline. The activity of SACs was strongly correlated with the presence of whole-cell
currents. Both the whole-cell currents and SACs were blocked by Gd3+ and by Grammostola spatulata spider venom. Mechanical stimulation of spontaneously active cells increased the beating rate and this effect was blocked
by Gd3+. We conclude that physiologically active mechanosensitive currents arise from stretch activated ion channels.
Received: 8 April 1996/Revised: 8 August 1996 相似文献
39.
Dr. Takashi Sugawara 《Cell and tissue research》1981,217(1):23-36
Summary A pair of multipolar stretch-receptive neurons were found in the bursa copulatrix of the female cabbage white butterfly, Pieris rapae crucivora. The cell body of each neuron, about 10 m in diameter, lies on the edge of the muscular region in the antero-lateral wall of the corpus bursae. No special accessory structure, such as a receptor muscle, is associated with the neuron. The several dendrites extend radially into the muscle layer. The dendrites are ensheathed except for their terminal tips, and, on their course, they anchor repeatedly on the epithelial cells or the muscle fibers in such a manner that their basement membranes fuse together. While the ensheathed dendrite is usually 0.1–0.2 m in diameter, it often forms 1–2 m varicosities especially at anchor sites, so that it looks like a varicose, or beaded, chain. The varicosities contain a number of mitochondria, but only microtubules are found in the fine interconnecting parts of the dendrite. The naked dendritic tips terminate in the basement membrane of the epithelial cell. The varicosities, as well as naked tips, seem to be important for stimulus transduction in the sensory cell of this type. 相似文献
40.
Janette Wallis 《International journal of primatology》1985,6(3):335-350
Recent findings suggest that menstrual synchrony may occur in humans and is more dependent on factors of close personal association
than simple prox imity. Part I of this study analyzed the estrous cycles of 10 captive group living chimpanzees (Pan troglodytes)
housed in two separate groups in the same building. Each group contained at least one male. The mean discrepan cies for (1)
the approximate day of ovulation (last day of maximum swelling) and (2) the day of onset of estrous swelling were determined
for all possible pairs within groups and compared to the mean discrepancies of pairs between groups. With respect to the probable
day of ovulation, the mean discrepancy of animals caged together was 8.0 days and that of animals caged separately was 10.
7 days. Comparing the onset of estrous swelling, the mean between groups was 10.4 days, whereas females caged together averaged
only 5.7 days. A repeated-measures analysis of variance indicated a significant group effect in the onset of estrous swelling
(F = 5.68,p < 0.05), whereas there was no significant difference between the animals caged together and those caged separately with respect
to the last day of maximum swelling (F = 1.37, NS). Part II of the study compared the onset of the first postpar tum estrus (PPE) to the onset of estrus in cagemates.
During a 6-year period, 13 females exhibited 33 PPEs while in social contact with cycling females. Of 86 paired comparisons,
the PPE onset of 43 (50%) occurred with in 6 days of the swelling onset of a cagemate. The results suggest that, as in other
species (including humans), the social contact and time spent together influence the synchrony of chimpanzee estrous cycles,
especially in terms of swelling onset. The synchrony of estrous swelling and possible mediation by olfactory and/or hormonal
cues are discussed as they relate to chimpanzee social structure and female reproductive strategy. 相似文献