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21.
An automated method for modeling proteins on known templates using distance geometry. 总被引:6,自引:5,他引:1
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S. Srinivasan C. J. March S. Sudarsanam 《Protein science : a publication of the Protein Society》1993,2(2):277-289
We present an automated method incorporated into a software package, FOLDER, to fold a protein sequence on a given three-dimensional (3D) template. Starting with the sequence alignment of a family of homologous proteins, tertiary structures are modeled using the known 3D structure of one member of the family as a template. Homologous interatomic distances from the template are used as constraints. For nonhomologous regions in the model protein, the lower and the upper bounds for the interatomic distances are imposed by steric constraints and the globular dimensions of the template, respectively. Distance geometry is used to embed an ensemble of structures consistent with these distance bounds. Structures are selected from this ensemble based on minimal distance error criteria, after a penalty function optimization step. These structures are then refined using energy optimization methods. The method is tested by simulating the alpha-chain of horse hemoglobin using the alpha-chain of human hemoglobin as the template and by comparing the generated models with the crystal structure of the alpha-chain of horse hemoglobin. We also test the packing efficiency of this method by reconstructing the atomic positions of the interior side chains beyond C beta atoms of a protein domain from a known 3D structure. In both test cases, models retain the template constraints and any additionally imposed constraints while the packing of the interior residues is optimized with no short contacts or bond deformations. To demonstrate the use of this method in simulating structures of proteins with nonhomologous disulfides, we construct a model of murine interleukin (IL)-4 using the NMR structure of human IL-4 as the template. The resulting geometry of the nonhomologous disulfide in the model structure for murine IL-4 is consistent with standard disulfide geometry. 相似文献
22.
Gaetano Barbato Daniel O. Cicero Elisabetta Bianchi Antonello Pessi Renzo Bazzo 《Journal of biomolecular NMR》1996,8(1):36-48
Summary We describe the high-resolution structure by NMR of two peptides that belong to a combinatorial library based on the zinc-finger motif. The library represents, to the best of our knowledge, the first example of a conformationally homogeneous peptide library and was obtained by introducing random residues in five positions of the -helical portion of a 26-residue consensus peptide (CP1) belonging to the Cys2-Hys2 zinc-finger family. The result was shown to be a highly homogeneous -helical library (Bianchi et al., 1995). The structures of the parent compound (CP1) and of a representative member (CP1m) that was selected by screening the library with a monoclonal antibody are compared in detail as an example of the very high stability of the zinc-finger scaffold upon sequence variability. The two peptides exhibit an extremely high degree of structural similarity. The use of this type of conformationally constrained combinatorial library might represent a step forward in the design of peptidomimetics, as it considerably accelerates the process of the identification of the spatial relationship among the pharmacophoric groups.Abbreviations
t-Bu
tert-butyloxycarbonyl
- Fmoc
9-fluorenylmethoxycarbonyl 相似文献
23.
Evolutionary analysis of the picornavirus family 总被引:1,自引:0,他引:1
An exhaustive evolutionary analysis of the picornavirus family has been carried out using the amino acid sequences of several proteins of the viruses including: the capsid proteins (1D, 1B, and 1C) situated at the 5 end of the genome and responsible for the serotype of the viruses, and the viral polymerase (3D), located at the 3 end of the genome. The evolutionary relationships found among the viruses studied support the new classification, recently suggested, in contrast to the classical one, and the existence of a new genus for the picornavirus family. In the new taxonomic organization, five genera form the picornavirus family: (1) aphthoviruses, (2) cardioviruses, (3) hepatoviruses (previously classified as enteroviruses), (4) renteroviruses (which mainly constitute a combination of the previous genera rhinovirus and enterovirus), and (5) a new genus, with a new and unique representative: the echovirus 22. Our analysis also allowed us, for the first time, to propose the most probable sequence of speciation events to have given rise to the current picornavirus family.The bootstrap procedure was used to check the reliability of the phylogenetic trees obtained. The application of the method of the statistical geometry in distance space to internal branches of the tree revealed a high degree of evolutionary noise, which makes the resolution of some internal branching points difficult.
Correspondence to: J. Dopazo 相似文献
24.
Xavier Isaac M. de Pádua Antônio Moraes Fernando De Miranda-Neto J. A. 《Molecular Engineering》1997,7(3-4):283-291
Starburst dendrimers are highly branched oligomers. A rigid dendritic hydrocarbon, C1134H1146, has recently been synthesized. It consists of 94 phenylacetylene units displayed in a self-similar two-dimensional skeleton
isomorphous to the three-coordinated Bethe lattice. The three-dimensional representation of phenylacetylene dendrimer shows
a globular architecture with large voids and niches in its interior, characteristic of hyperbolic surfaces. This work investigates
the geometrical scaling behavior of this starburst dendrimer using the symmetry properties of a Bethe lattice embedded in
the hyperbolic plane. The results for C1134H1146 provide its density profile and an upper bound for its macromolecular size.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
25.
Adaptation of wastewater surface flow wetland formulae for application in constructed stormwater wetlands 总被引:1,自引:0,他引:1
Over the past 30 years, the use of constructed wetlands for wastewater treatment has been a topic of significant research culminating in a good data base from which simplistic equations have been derived to aid in the design of these facilities to meet long term water quality treatment performance criteria. Over the past decade, the use of treatment wetlands has extended to stormwater and combined sewer overflow (CSO) management applications. Designing constructed wetlands for stormwater and CSO applications have unique challenges stemming from the highly stochastic nature of the hydraulic and pollutant loading on a stormwater wetland compared with wastewater treatment systems. This paper explores the possibility of adapting the simplistic models for wastewater wetlands for interim use in developing design guidelines for stormwater wetland systems. A procedure that takes into account the unsteady intermittent nature of stormwater inflows to these wetlands has been incorporated into one of these simplistic models and a case study presented to demonstrate the application of the procedure. 相似文献
26.
The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. 总被引:1,自引:1,他引:0
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E. Ab G. Schuurman-Wolters J. Reizer M. H. Saier K. Dijkstra R. M. Scheek G. T. Robillard 《Protein science : a publication of the Protein Society》1997,6(2):304-314
The assignment of the side-chain NMR resonances and the determination of the three-dimensional solution structure of the C10S mutant of enzyme IIBcellobiose (IIBcel) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli are presented. The side-chain resonances were assigned nearly completely using a variety of mostly heteronuclear NMR experiments, including HCCH-TOCSY, HCCH-COSY, and COCCH-TOCSY experiments as well as CBCACOHA, CBCA(CO)NH, and HBHA(CBCA)(CO)NH experiments. In order to obtain the three-dimensional structure, NOE data were collected from 15N-NOESY-HSQC, 13C-HSQC-NOESY, and 2D NOE experiments. The distance restraints derived from these NOE data were used in distance geometry calculations followed by molecular dynamics and simulated annealing protocols. In an iterative procedure, additional NOE assignments were derived from the calculated structures and new structures were calculated. The final set of structures, calculated with approximately 2000 unambiguous and ambiguous distance restraints, has an rms deviation of 1.1 A on C alpha atoms. IIBcel consists of a four stranded parallel beta-sheet, in the order 2134. The sheet is flanked with two and three alpha-helices on either side. Residue 10, a cysteine in the wild-type enzyme, which is phosphorylated during the catalytic cycle, is located at the end of the first beta-strand. A loop that is proposed to be involved in the binding of the phosphoryl-group follows the cysteine. The loop appears to be disordered in the unphosphorylated state. 相似文献
27.
Souichi Morikawa Kazuhiro Ogata Ai Sekikawa Akinori Sarai Shunsuke Ishii Yoshifumi Nishimura Haruki Nakamura 《Journal of biomolecular NMR》1995,6(3):294-305
Summary The solution structure of a specific DNA complex of the minimum DNA-binding domain of the mouse c-Myb protein was determined by distance geometry calculations using a set of 1732 nuclear Overhauser enhancement (NOE) distance restraints. In order to determine the complex structure independent of the initial guess, we have developed two different procedures for the docking calculation using simulated annealing in four-dimensional space (4D-SA). One is a multiple-step procedure, where the protein and the DNA were first constructed independently by 4D-SA using only the individual intramolecular NOE distance restraints. Here, the initial structure of the protein was a random coil and that of the DNA was a typical B-form duplex. Then, as the starting structure for the next docking procedure, the converged protein and DNA structures were placed in random molecular orientations, separated by 50 Å. The two molecules were docked by 4D-SA utilizing all the restraints, including the additional 66 intermolecular distance restraints. The second procedure comprised a single step, in which a random-coil protein and a typical B-form DNA duplex were first placed 70 Å from each other. Then, using all the intramolecular and intermolecular NOE distance restraints, the complex structure was constructed by 4D-SA. Both procedures yielded the converged complex structures with similar quality and structural divergence, but the multiple-step procedure has much better convergence power than the single-step procedure. A model study of the two procedures was performed to confirm the structural quality, depending upon the number of intermolecular distance restraints, using the X-ray structure of the engrailed homeodomain-DNA complex.Abbreviations rmsd
root-mean-square deviation
- NOE
nuclear Overhauser enhancement
- 4D-SA
simulated annealing in four-dimensional space
- Myb-R2R3
repeats 2 and 3 of the DNA-binding domain of the c-Myb protein
- DNA 16
Myb-specific binding DNA duplex with 16 base pairs
- IHDD-C
residues 3 to 59 of the C-chain of the engrailed homeodomain-DNA complex
- DNA11
DNA duplex with base pairs 9 to 19 of the engrailed homeodomain-DNA complex 相似文献
28.
Effect of chloride withdrawal on the geometry of the T-tubules in amphibian and mammalian muscle 总被引:3,自引:0,他引:3
Angela Dulhunty 《The Journal of membrane biology》1982,67(1):81-90
Summary The relative chloride permeabilities of the T-tubule membranes in mammalian (rat sternomastoid) and amphibian (toad sartorius) skeletal muscle fibers have been assessed from the change in volume of the T-tubules during chloride withdrawal from fibers exposed to low extracellular chloride concentrations. A 3.5- to 4.2-fold increase in T-tubule volume was found in mammalian fibers, and this was shown to be independent of the composition of the low chloride solution or the nature of the fixative used in preparation for electron microscopy. The increase in T-tubule volume was transient and was inhibited by factors which block chloride conductance, i.e., low pH, 2,4-dichlorophenoxyacetic acid, and nitrate ions. A small increase (1.48-fold) in T-tubule volume was seen in amphibian fibers when chloride ions were replaced by sulphate ions. No increase in volume was observed in amphibian T-tubules when methyl sulphate ions replaced chloride ions. The results support the idea that the chloride permeability of the T-tubule membrane is significantly higher in mammalian fibers than in amphibian fibers. 相似文献
29.
H. -R. Gregorius 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1980,56(4):187-192
Summary A model for positive assortative mating based on genotype for one locus is employed to investigate the effect of this mating system on the genotypic structure of a second linked locus as well as on the joint genotypic structure of these two loci. It is shown that the second locus does not attain a precise positive assortative mating structure, but yet it shares a property that is characteristic of positive assortative mating, namely an increase in the frequency of homozygotes over that typically found in panmictic structures. Given any arbitrary genotypic structure for the parental population, the resulting offspring generation possesses a structure at the second locus that does not depend on the recombination frequency, while the joint structure of course does. In case assortative mating as well as linkage are not complete, there exists a unique joint equilibrium state for the two loci, which is characterized by complete stochastic independence between the two loci as well as by Hardy-Weinberg proportions at the second locus. For the second locus alone, Hardy-Weinberg equilibrium is realized if and only if gametic linkage equilibrium and an additionally specified condition are realized. 相似文献
30.
Responses to selection on genotypic or phenotypic values in the presence of genes with major effects
E. Sehested I. L. Mao 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,85(4):403-406
Summary Average genotypic responses were compared after selection for genotypic values and for phenotypic values on the basis of single-gene models and multigene models in simulated livestock populations. Single-gene models dealt with single gene control of the genetic differences between animals, while multigene models considered a collection of genes with various magnitudes of effects on a trait. In each case, selection lasted through discrete generations until the fixation of the gene frequencies occurred. Generations to reach fixation were used to compare various models, and the two criteria for selection, for their efficiency in selection. Implications of using these models versus using infinitesimal models for selection in practice are presented. 相似文献