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151.
152.
Cellular energy metabolism maintains young status in old queen honey bees (Apis mellifera) 下载免费PDF全文
Cheng‐Yen Lu Jiantai Timothy Qiu Chin‐Yuan Hsu 《Archives of insect biochemistry and physiology》2018,98(4)
Trophocytes and oenocytes of queen honey bees are used in studies of cellular longevity, but their cellular energy metabolism with age is poorly understood. In this study, the molecules involved in cellular energy metabolism were evaluated in the trophocytes and oenocytes of young and old queen bees. The findings indicated that there were no significant differences between young and old queen bees in β‐oxidation, glycolysis, and protein synthesis. These results indicate that the cellular energy metabolism of trophocytes and oenocytes in old queen bees is similar to young queen bees and suggests that maintaining cellular energy metabolism in a young status may be associated with the longevity of queen bees. Fat and glycogen accumulation increased with age indicating that old queen bees are older than young queen bees. 相似文献
153.
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system 总被引:1,自引:0,他引:1
Elizabeth W. Davidson Rufino B. R. Patron Lawrence A. Lacey Roger Frutos Alain Vey Donald L. Hendrix 《Entomologia Experimentalis et Applicata》1996,79(1):25-32
An assay system was developed for the adult silverleaf whitefly, Bemisia argentifolii Bellows & Perring (Homoptera: Aleyrodidae). This practical device was constructed from standard disposable laboratory materials. Whiteflies were harvested directly from the leaf and into a collection vial by vacuum aspiration, minimizing physical damage to the insect. Insects were fed through a cellulose mixed-ester membrane on a diet of 20–27% sucrose alone or sucrose in an extract of zucchini (Curcurbita moschata Duchense). Mortality and honeydew production were scored. At 22–25°C and 50–55% relative humidity, control mortality generally remained at or below 15% during a 48 h assay period. The bioassay system was first tested using the insecticide, Imidacloprid, then used to screen a number of natural products with potential insecticidal activity against the whitefly. Destruxins extracted from the entomopathogenic fungus, Metarhizium anisopliae, and the natural insecticide/nematicide, Ivermectin, as well as bee venom and two of its components, melittin and phospholipase A2, were found to be toxic to B. argentifolii. Five lectins, Bacillus thuringiensis toxins, gossypol, an extract of Paeciliomyces fumosoroseus, wasp and scorpion venom, and a trypsin inhibitor were not found to be insecticidal to adult B. argentifolii. 相似文献
154.
Byrsonima rrassifolia (L.), a Neotropical malpighiaceous tree or treelet, has flowers that produce abundant lipids instead of nectar. Several species of Centris bees collect this oil. The floral oil shows variation between trees and can be separated into two types. One type is most common (11 of 14 samples) and consists of mono- and di-glycerides, some free fatty acids, a small amount of tri-glycerides and a trace of carbohydrate. The composition of the glycerides is predominantly (53–63%) esters of unsaturated 18C–22C fatty acids with the rest made up of 14C–18C saturated fatty acids. The second type of floral oil is similar to type one except that the samples contain large amounts of an unknown lipid more polar than the standards. Oil collected from the scopae of two representative Centris species, C. adanae and C. flavofasciata, was the same as the type one oil collected from B. rrassifolia floral elaiophores. The liquid provisions from the cells of several groundnesting Centris species was also found to be identical to B. crassifolia type one floral elaiophore oil with the exception of several minor ninhydrin positive compounds which may have been derived from the pollen which makes up part of the larval food provisions. 相似文献
155.
By setting out arrays of potted plants of Penstemon strictus,I tested whether freely foraging bumblebee (Bombus spp. ) workerswould establish regular foraging routes that reflected the geometryof the array. They did, passing through an asymmetrical arrayin a pattern that minimized interplant flight distances. Afterthe array was changed to a symmetrical pattern, however, theexperienced bees continued to show their previous asymmetricalflight patterns. New bees without experience on the asymmetricalarray showed no asymmetry on the symmetrical array. I term thispersistence of flight-path geometry "trapline holdover, " anddiscuss its implications for the study of animals' learningand foraging behavior. 相似文献
156.
Local bumble bee decline linked to recovery of honey bees,drought effects on floral resources 下载免费PDF全文
Diane M. Thomson 《Ecology letters》2016,19(10):1247-1255
Time series of abundances are critical for understanding how abiotic factors and species interactions affect population dynamics, but are rarely linked with experiments and also scarce for bee pollinators. This gap is important given concerns about declines in some bee species. I monitored honey bee (Apis mellifera) and bumble bee (Bombus spp.) foragers in coastal California from 1999, when feral A. mellifera populations were low due to Varroa destructor, until 2014. Apis mellifera increased substantially, except between 2006 and 2011, coinciding with declines in managed populations. Increases in A. mellifera strongly correlated with declines in Bombus and reduced diet overlap between them, suggesting resource competition consistent with past experimental results. Lower Bombus numbers also correlated with diminished floral resources. Declines in floral abundances were associated with drought and reduced spring rainfall. These results illustrate how competition with an introduced species may interact with climate to drive local decline of native pollinators. 相似文献
157.
158.
J.A. Svoboda M.J. Thompson E.W. Herbert H. Shimanuki 《Journal of insect physiology》1980,26(5):291-294
The sterols of prepupal honey bees, Apis mellifera L., from brood reared by workers fed chemically-defined synthetic diets containing cholesterol, campesterol, sitosterol, stigmasterol, 24-methylenecholesterol, or no sterol over a 12-week period were isolated, identified, and quantified. The major sterol present in each prepupal sample was 24-methylenecholesterol, but significant levels of sitosterol and isofucosterol were also present in every case, as was a very small percentage of desmosterol (usually < 1%). This is the first report of isofucosterol being identified in the sterols of the honey bee. A considerably larger percentage of each dietary sterol was found in prepupae reared by workers fed that particular sterol in the diet. This was most dramatic in the case of the cholesterol diet in which case cholesterol content increased to as much as 17.2% of the prepupal sterols, whereas cholesterol had not exceeded 2.2% in samples from other diet regimens. However, stigmasterol comprised no more than 6.3% of the total sterols in any sample from prepupae fed the stigmasterol diet. The preponderance of 24-methylenecholesterol in all prepupae, regardless of the dietary sterol provided to the workers, as well as the lesser quantities of sitosterol and isofucosterol present in all samples, suggest a unique system of utilization and metabolism of these dietary sterols by the worker bees. Apparently they make available to the brood varying amounts of unchanged dietary sterol plus considerable and fairly constant portions of 24-methylenecholesterol, sitosterol, and isofucosterol drawn from their own sterol pools. 相似文献
159.
The visitation pattern by worker honey bees to cells in the brood nest was monitored on an artificially created brood pattern consisting of about one-fourth brood cells evenly distributed among empty cells. The majority (63 %) of the observed workers selectively entered larval cells. In contrast, some workers avoided egg cells when presented a choice of egg vs empty cells. The results suggest that larvae produce a general signal indicating their presence to worker bees. Eggs also seem to produce a signal, which is perceived to be different from the one from larvae. 相似文献
160.
Oxygen consumption and flight muscle activity during heating in workers and drones of Apis mellifera
Franz Goller Harald E. Esch 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1991,161(1):61-67
Summary Instantaneous oxygen consumption, muscle potential frequency, thoracic and ambient temperature were simultaneously measured during heating in individual workers and drones of honey bees. Relationships between these parameters and effects of thoracic temperature on power input and temperature elevation were studied. Oxygen consumption increased above basal levels only when flight muscles became active. Increasing muscle potential frequencies correlated with elevated oxygen consumption and raised thoracic temperature. The difference between thoracic and ambient temperature and oxygen consumption were linearly related. Oxygen consumption per muscle potential (l O2 · g
–1
thorax
· MP–1) was two-fold higher in drones than in workers. However, oxygen consumption for heating the thorax (l O2 · g
–1
thorax
· (Tth-Ta) · °C–1) was nearly the same in workers and drones. Thoracic temperature affected the amount of oxygen consumed per muscle potential (R10=1.5). Achieved temperature elevation per 100 MP was more temperature sensitive in drones (R10=6–10) than in workers (R10=3.6). Q10 values for oxygen consumption were 3 in workers and 4.5–6 in drones. Muscle potential frequency decreased with a Q10=1.8 in workers and 2.7 in drones. Heating behaviour of workers and drones was different. Drones generated heat less continuously than workers, and showed greater interindividual variability in predilection to heat. However, the maximal difference between ambient and thoracic temperature observed was 22 °C in drones and 14 °C in workers, indicating greater potential for drones.Abbreviations
DL
dorsal-longitudinal muscle
-
DV
dorsoventral muscle
-
MP
muscle potential
-
T
a
ambient temperature
-
T
th
thoracic temperature 相似文献