全文获取类型
收费全文 | 11610篇 |
免费 | 624篇 |
国内免费 | 1911篇 |
专业分类
14145篇 |
出版年
2024年 | 23篇 |
2023年 | 197篇 |
2022年 | 310篇 |
2021年 | 386篇 |
2020年 | 313篇 |
2019年 | 403篇 |
2018年 | 340篇 |
2017年 | 297篇 |
2016年 | 293篇 |
2015年 | 363篇 |
2014年 | 610篇 |
2013年 | 648篇 |
2012年 | 480篇 |
2011年 | 665篇 |
2010年 | 576篇 |
2009年 | 652篇 |
2008年 | 694篇 |
2007年 | 666篇 |
2006年 | 623篇 |
2005年 | 570篇 |
2004年 | 534篇 |
2003年 | 524篇 |
2002年 | 377篇 |
2001年 | 325篇 |
2000年 | 298篇 |
1999年 | 266篇 |
1998年 | 259篇 |
1997年 | 216篇 |
1996年 | 196篇 |
1995年 | 211篇 |
1994年 | 214篇 |
1993年 | 190篇 |
1992年 | 192篇 |
1991年 | 127篇 |
1990年 | 129篇 |
1989年 | 113篇 |
1988年 | 81篇 |
1987年 | 91篇 |
1986年 | 75篇 |
1985年 | 91篇 |
1984年 | 67篇 |
1983年 | 62篇 |
1982年 | 84篇 |
1981年 | 45篇 |
1980年 | 53篇 |
1979年 | 57篇 |
1978年 | 58篇 |
1977年 | 22篇 |
1976年 | 26篇 |
1974年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
M. L. Fisher M. M. Kyle 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(2):204-212
We have examined the genetics of systemic resistance in Phaseolus vulgaris to azuki bean mosaic virus (AzMV) and cowpea aphid-borne mosaic virus (CABMV) and the relationship of this resistance to a phenotypically similar resistance to watermelon mosaic virus (WMV) and soybean mosaic virus (SMV). In P. vulgaris cv Great Northern 1140 (GN1140), resistance to SMV and WMV has been attributed to the genes Smv and Wmv, respectively, which have been shown to segregate as a unit. Systemic resistance to AzMV is conferred by two incompletely dominant alleles, Azm1 and Azm2, at unlinked loci. At least three resistance alleles must be present at these two loci for systemic resistance to be expressed in the plant. Systemic resistance to CABMV in GN 1140 is conditioned by a dominant allele that has been designated Cam2. Under some environmental conditions, a recessive allele at an unlinked locus, cam3, also controls a resistant response to CABMV. Resistance to AzMV and CABMV does not assort independently from Wmv/Smv, but also does not consistently cosegregate, suggesting that perhaps in each case one of the factors involved in resistance is associated with Smv/Wmv. 相似文献
32.
本文比较了用HSV-1(IEA)和B病毒(IFA)的方法检查猴B病毒相关抗体和B病毒抗体的结果:IEA检查出的B病毒相关抗体阴性猴,经IFA检查,全部为B病毒抗体阴性。用IFA检查了B病毒相关抗体阴性的恒河猴,在单笼隔离饲养六个月后,有98.3%的动物B病毒相关抗体仍为阴性,表明IRA的阴性结果有较好的一致性。本文讨论了建立无B病毒感染猴群的可行性。 相似文献
33.
Abstract: Derivatives of the lac promoter (tac, pac, rac) belong to the strongest bacterial promoters which are frequently used for the induced overexpression of foreign genes in Escherichia coli . However, their use in fermentation processes is strongly restricted because of the high cost of the inducer iso-propyl-β-D-thiogalactopyranoside (IPTG). The aim of this work was to investigate the possibility of using lac-derived promoters in high cell density processes resulting in a high yield of the induced recombinant protein if glucose is the main carbon and energy source. Lactose is tested as inducer of the main antigenic coat protein (VP1) of the foot and mouth disease (FMD) virus in a T7-RNA polymerase expression system. It was shown that lactose is able to induce the expression of the recombinant gene to an amount of the VP1 protein corresponding to 20% of the total cell protein. 相似文献
34.
Abstract.
- 1 The effects of resource limitation and the lethal and sublethal effects of a granulosis virus on a lepidopteran host, the Indian meal moth, Plodia interpunctella, were examined.
- 2 The food quality was manipulated by the addition of an inert bulking agent (methyl cellulose) which caused the size, development rate and fecundity of the moths to be reduced.
- 3 The resource quality had no effect on the mortality due to the virus. In contrast, sublethal effects of the virus on pupal weight were more apparent under conditions of resource limitation.
- 4 Considerable variation between the sublethal effects after challenge with different doses of the virus was found. The balance between deleterious sublethal effects of the virus and the selection of more robust individuals by the bioassays is proposed as a mechanism to explain this variation.
- 5 Implications for the dynamics of insect hosts and their pathogens are discussed.
35.
ts7, a temperature-sensitive mutant defective in neuraminidase (NA) of influenza B/Kanagawa/73, lacks NA enzymatic activity at the nonpermissive temperature (37.5 C). When MDCK cells were infected with the mutant at the permissive temperature (32 C) and exposed to pH 5.2 medium, extensive cell fusion occurred. In contrast, at the nonpermissive temperature cells did not show cell fusion at all unless they were pretreated with trypsin, suggesting that at 37.5 C the hemagglutinin (HA) of ts7 is expressed at the cell surface in an uncleaved form. It was also found that the replacement of RNA segment 6 of ts7 with that of wild-type B/Lee resulted in the emergence of low pH-induced fusion activity as well as NA enzymatic activity at the incubation temperature of 37.5 C and that the addition of bacterial NA to the cultures infected with ts7 at 37.5 C early in infection brought about low pH-induced cell fusion. We suggest that the removal of neuraminic acid from the carbohydrate moiety of HA by NA is essential for the cleavage of HA by cellular protease. 相似文献
36.
37.
Sieghart Sopper Susanne Hemm Jürgen Meixensberger Cheik Coulibaly Christiane Stahl-Hennig Gerhard Hunsmann Bernhard Fleckenstein Volker ter Meulen Rüdiger Drries 《Journal of medical primatology》1993,22(2-3):138-146
Paired sera and CSF samples were collected from SIVmac-infected macaques. Animals infected with SIVmac251 maintained low gag and high env-specific antibody levels in plasma. Increasing env-specific antibody titers in CSF were associated in one animal with strong intrathecal synthesis. SIVmac239-infected monkeys revealed high antibody titers of gag and env-specificity, in one animal accompanied by weak intrathecal synthesis of virus-specific antibodies. In all animals, the CD4/CD8 ratio in CSF decreased faster compared to blood. 相似文献
38.
39.
Use of VIRAZOLE® to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw. 总被引:1,自引:0,他引:1
A. Toussaint J. Kummert C. Maroquin A. Lebrun J. Roggemans 《Plant Cell, Tissue and Organ Culture》1993,32(3):303-309
Odontoglossum Ringspot Virus has been eradicated from Cymbidium Sw. through chemotherapy based on incorporation of ribavirin (VIRAZOLE®) into the in vitro culture medium of protocorms. Applications of the virustatic agent for several consecutive subcultures freed protocorms of the virus. Acclimated plantlets regenerated from those protocorms are healthy as determinated by enzyme-linked immunosorbent assay (ELISA). No resurgence of virus occurred over a period of 5 years. Besides, trueness to type was total at flowering level and the batch grown was perfectly homogeneous.To secure fast and effective eradication of the virus during the consecutive subcultures of protocorms with ribavirin, three factors proved to be of prime importance: accurate isolation of new growths from initial tissues, VIRAZOLE® concentration and frequency of transplanting in new media. 相似文献
40.
Study of multiplication of cucumber mosaic virus in susceptible and resistant Capsicum annuuum lines
R. NONO-WOMDIM K. GEBRE-SELASSIE A. PALLOIX E. POCHARD G. MARCHOUX 《The Annals of applied biology》1993,122(1):49-56
A previous survey on pepper lines (Capsicum annuum L.) indicated that a susceptible cultivar, Yolo Wonder, reacted to cucumber mosaic virus (CMV) by producing a systemic yellow mosaic. By contrast, CMV caused no symptoms on lines Perennial and Vania. The virus is recoverable from the uninoculated leaves of Perennial, while in Vania CMV is restricted to the inoculated leaves. To interpret these phenomena, a comparative study on CMV multiplication rates, yield, specific infectivity and relative proportion of RNAs was made in the inoculated leaves of the three pepper varieties. The rate of CMV multiplication, as estimated by the double antibody sandwich form of enzyme-linked immu-nosorbent assay, was lower in Perennial than in Vania or Yolo Wonder. The yield of virus purified from Perennial was very low when compared with Vania or Yolo Wonder. The specific infectivity of the virus extracted from Perennial was less than that from Vania or Yolo Wonder. These results suggest that Perennial is resistant to CMV multiplication, while restriction of the virus in inoculated leaves of Vania is not due to the inhibition of the virus replication. However, polyacrylamide gel electrophoresis revealed that the RNA profiles of CMV purified from the three pepper lines were similar. 相似文献