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101.
A monoclonal antibody was used to localize abscisic acid in Lavandula stoechas L. plants treated with 1 M abscisic acid. ABA localization was performed using EDC as the only fixative, which preserves antigenicity and improves the specificity of monoclonal antibodies. A relationship was observed between the effect of abscisic acid on chloroplast ultrastructure and ABA accumulation. Gold particles accumulated on swollen chloroplasts. Our findings provide cytochemical evidence that the chloroplast is a trapping compartment, and yield valuable information on the relation between chloroplast ultrastructure and ABA accumulation.Abbreviations ABA
abscisic acid
- BSA
bovine serum albumin
- EDC
1-(3-dimethyl-aminopropyl)-3 ethyl carbodiimide
- IgG
immunoglobulin G
- mAB
monoclonal antibodies
- pAB
polyclonal antibodies 相似文献
102.
Nicoletta Zini Katia Scotlandi Nicola Baldini Giuseppe Nini Patrixia Sabatelli Nadir M Maraldi 《Biology of the cell / under the auspices of the European Cell Biology Organization》1995,84(3):195-204
Summary— Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morphological changes associated with MDR have been evaluated by quantitative image analysis and transmission electron microscopy. Resistant cells present morphological changes with respect to sensitive cells at both cytoplasmic and nuclear level. Some of these changes appear to be related to the degree of resistance but not to the direct presence of DX, since deprived cells maintain some modified characters, while others are partly lost. These findings suggest that DX exposure affects cell metabolism causing progressive changes of the cell morphotype. 相似文献
103.
Aspects of the phylogeny of Platyhelminthes based on 18S ribosomal DNA and protonephridial ultrastructure 总被引:2,自引:2,他引:0
DNA studies of 23 taxa (20 platyhelminths, 1 nemertean, Homo and Artemia) and electron-microscopic studies of the protonephridia of many platyhelminths (supported by some additional ultrastructural data) have led to the following conclusions: the Neodermata are monophyletic; Temnocephalida and Dalyelliida form one clade and are not the primitive sister group of the Neodermata; Gyrocotylidea, Amphilinidea and Eucestoda form one monophylum; Pterastericolidae and Umagillidae are dalyelliids and not the sister group of the Neodermata; and Proseriata are unlikely to be closely related with the Tricladida. A large taxon consisting of the Proseriata and some other turbellarians may represent the sister group of the Neodermata. 相似文献
104.
S. A. Smith T. Caceci † H. E-S. Marei ‡ H. A. El-Habback § 《Journal of fish biology》1995,46(2):241-254
In the angelfish ( Pterophyllum scalare scalare ) numerous rodlet cells were found in the large post-orbital blood vessel caudal to the eye and in the surrounding extravascular space. Within the vessel the rodlet cells formed striking regular arrays, along the inner aspect of the wall. The rodlets within the cells were positive to PAS but negative to Sudan Black B, Masson's, and the Fuelgen stain. The capsule around the cells was negative for all these stains. These rodlet cells appeared to be traversing the vessel endothelium, and to be pushing the endothelium aside without damaging it. Some discharged their contents into the vessel, but we never observed the release of intact rodlets. The nuclei of rodlet cells in actual contact with the vessel were at the end of the cell more distant from the endothelial wall. Cell-to-cell adhesion structures or communications junctions between rodlet cells and the endothelium were not evident. A putative rodlet cell precursor in the extravascular space contained large electron-dense granules, and extended pseudopodia that contacted nearby rodlet cells. Based on their morphology, tissue distribution, and their behaviour, we conclude that the rodlet cell is an endogeneous teleost cell type, and possibly represents a form of matured granulocyte. 相似文献
105.
The division Haptophyta is represented only by about 300 extant species showing wide diversity in morphology, biochemistry and ecology. They have a world-wide distribution and are numerically important in phytoplankton populations in nearly all marine environments. Evidence from the geological record shows that they have been the major constituent of calcareous deposits since the Late Triassic and, as they have evolved quickly through time, their coccoliths have always shown wide morphological diversity. In today's oceans they occasionally produce extensive blooms, visible by satellite imagery, which have ecological impact. As a consequence of these blooms the haptophyte algae are now receiving greater attention, as their role in the global sulphur and carbon cycles may influence the world's climate, and their potential as nuisance bloom algae have implications for commercial fishing and the marine ecosystem. As it is likely that these organisms have always produced such blooms, these effects may have been in operation for the last 200 million years. 相似文献
106.
The flagellin gene of Borrelia miyamotoi sp. nov. and its phylogenetic relationship among Borrelia species 总被引:1,自引:0,他引:1
Abstract We have cloned and sequenced the flagellin gene from Borrelia miyamotoi strain HT31 and compared it with previously published flagellin sequences. Sequence similarity analysis demonstrated that strain HT31 is phylogenetically distant from the three species of Lyme disease borreliae and is deeply branched into the relapsing fever borrelia cluster. The result was in full agreement with the classification of Borrelia strains using 16S rRNA sequences. This finding indicates that a phylogenetic analysis using flagellin gene sequences might be useful for classification of Borrelia strains. 相似文献
107.
M. Grilli Caiola 《Plant Systematics and Evolution》1995,198(3-4):155-166
Crocus cartwrightianus andC. cartwrightianus cv.albus pollen have been studied from structural and ultrastructural points of view and the germination assayed in vivo and in vitro.C. cartwrightianus pollen is regularly shaped and sized, has a low percentage of anomalous grains and has a high germination rate in vitro, whileC. cartwrightianus cv.albus is less regularly shaped with some variation in size and has a high percentage of anomalous grains and a low germination percentage in vitro. Ultrastructural observations have revealed, in the pollen of both the taxa, the presence of a thin elongated vegetative nucleus and a generative cell surrounded by a thin membrane. However,C. cartwrightianus pollen shows a thicker intine, andC. cartwrightianus cv.albus shows numerous pollen germination anomalies which are in common withC. sativus. 相似文献
108.
Tree decline and deaths have been observed among 15 to 20-year-old Scots pines (Pinus sylvestris L.) in a dry heath forest in southwestern Finland. The sudden decline in height growth, the dieback of leading shoots and the yellowing of needles in young shoots in the upper part of the tree are typical symptoms of the decline of these young pines. Needle ultrastructure and chemical composition of Scots pines with or without decline and fluctuations of them in different seasons were studied. Afflicted trees were found to suffer from a deficiency in calcium and magnesium with low concentration of foliar nitrogen and phosphorus observed in all the trees studied. Ultrastructural study revealed changes characteristic of different seasons and measured nutrient status of needles. A clear reduction of membrane system in chloroplasts, especially related to Mg deficiency, was observed in most samples. The symptoms related to N deficiency, the translucent appearance of the cytoplasm and chloroplast stroma, and the elongated chloroplasts, as well as swelling of mitochondria, indicating P deficiency, were also found in the needles sampled from this forest decline area. The present study showed that it is possible to detect specific nutrient deficiency symptoms in needle ultrastructure in field samples and for use as sensitive indicators of unbalanced nutrient status. 相似文献
109.
鹅掌楸属植物引导组织和花粉管生长 总被引:9,自引:0,他引:9
应用光学显微镜和常规石蜡切片技术研究了鹅掌楸属(LiriodendronL.)两种植物雌蕊引导组织的分布和个体发育,引导组织是由心皮边缘或内表面的表皮细胞层或亚细胞层发育形成,是由一层细胞组成的连续层,覆盖干柱头、花柱道和珠柄的表面,引导组织的细胞形态学因其所在部位不同而有差异。在电境水平上研究了柱头和花柱引导组织的超微结构,引导组织细胞是分泌型的传递细胞,其分泌面发育了明显的壁内突,细胞质中富含内质网、多聚核糖体、各种小泡、高尔基体和线粒体,大液泡通常远离分泌面。文中还探讨了花粉管生长后引导组织的变化。 相似文献
110.
Lynn R. Trusal Albert W. Guzman Carol J. Baker 《In vitro cellular & developmental biology. Plant》1984,20(4):353-364
Summary The pathophysiology of endothelial cells is important to a variety of vascular conditions including coagulation and hemostasis
resulting from clinical frostbite. Use of an in vitro model system demonstrated that when bovine endothelial cells were frozen
at 1°C or 20°C/min and thawed immediately (20°C/min), a variety of ultrastructural alterations occurred. Membraneous structures
were most extensively damaged, with mitochondria the most sensitive organelle. Low amplitude mitochondrial swelling, first
evident at 0°C, progressed to high amplitude swelling by −10°C (frozen). In addition, the rough endoplasmic reticulum was
dilated and formed large vesicles with a homogeneous matrix. Nuclear changes first occurred at −15°C. These included separation
and distortion of the nuclear membrane, changes in chromatin distribution, and disruption of the nucleolus. Scanning electron
microscopy revealed perforated plasma membranes in some cells at −10°C (frozen) and in most cells by −20°C. Cultures frozen
at 20°C/min revealed mostly the same ultrastructural damage noted at 1°C/min except a higher percentage of cells exhibited
alterations. Data from the recovery index and lactic dehydrogenase (LDH) release correlated well with observed ultrastructural
changes. Early swelling of mitochondria and dilation of rough endoplasmic reticulum was not lethal in the absence of freezing.
Increased swelling in cytoplasmic organelles coupled with nuclear alterations at −15°C resulted in a decreased survival rate
and release of significant quantities of LDH by −20°C. No unique morphological changes were temperature specific, but the
total number of cells that displayed alterations increased as temperature decreased.
The views, opinions or findings, or both, contained in this report are those of the authros and should not be construed as
indicative of an official Department of the Army position, policy, or decision unless so designated by other official documentation. 相似文献