Spatial studies of colonization and succession of soil algae and chemical analyses of the various soils on the cinde cone of Kilauea Iki in Hawaii Volcanoes National Park, Hawaii are outlined. There is a positive correlation between the diversity and quantity of soil algae with nutrient levels and organic matter accumulation in each locale. Three distinct edaphic-biotic zones existing in this area are differentially revealed by the soil chemical composition, quantity and diversity of soil algae, and as evident variations in higher plant growth and colonization. Varying colonization and successional phases of higher plant growth around standing and fallen tree snags killed by volcanic activity also reflect variations in the soil algal flora. These variations appear largely as a function of differential water interception, absorption, and retention as well as differential accumulation of organic matter, and the initiation of various biogeochemical cycles. 相似文献
Summary Dissociated normal mammary epithelial cells from prelactating mice were plated on different substrates in various medium-serum-hormone
combinations to find conditions that would permit maintenance of morphological differentiation. Cells cultured on floating
collagen membranes in medium containing insulin, hydrocortisone and prolactin maintain differentiation through 1 month in
culture. The surface cells form a continuous epithelial pavement. Some epithelial cells below the surface layer rearrange
themselves to form alveolus-like structures. Cells at both sites display surface polarization; microvilli and tight junctions
are present at their medium-facing or luminal surface and a basal lamina separates the epithelial components from the gel
and stromal cells. Occasinal myoepithelial cells, characterized by myofilaments and plasmalemmal vesicles, are identified
at the basal surface of the secretory epithelium. In contrast, cells cultured on plastic, glass or collagen gels attached
to Petri dishes form a confluent epithelial sheet showing surface polarization, but lose secretory and myoepithelial specializations.
If these dedifferentiated cells are subsequently maintained on floating collagen membranes, they redifferentiate. There is
little DNA synthesis in cells on collagen gels, in contrast to Petri-dish controls. Protein synthesis in cells on floating
collagen membranes increases over T0 values and remains constant through 7 days in culture whereas it decreases on attached gels; however, if the gels are freed
to float, protein synthesis increases sharply and parallels that seen on floating membranes.
The work was supported by USPHS Grants CA-05388 and CA-05045 from the National Cancer Institute, DHEW. 相似文献
1. 1.|The purpose of this study was to determine the threshold specific absorption rate (SAR) during exposure to 2450 MHz continuous wave (CW) microwaves that affected thermoregulatory behaviour in mice.
2. 2.|A Plexiglas shuttle box was placed inside a waveguide imposed with a temperature gradient. The temperature gradient allowed the mice to select a particular section of the shuttle box which was, presumably, related to their state of thermal comfort. Exposing the mice to 2450 MHz inside the waveguide at SARs of 0–5.3 W kg−1 for 1 h caused no significant change in their preferred ambient temperature.
3. 3.|Increasing SAR from 5.3 to 18.1 W kg−1 caused the animals to shift their position to the cooler end of the shuttle box.
4. 4.|Following termination of microwave exposure animals that had selected a cool ambient temperature returned to the warm side of the shuttle box.
5. 5.|It is concluded that for mice exposed to radiation at 2450 MHz the thermoregulatory behaviour is significantly affected at SARs of 5.3 to 9.9 W kg−1.
Summary Rare mutations that alter the substrate specificity of proline permease cluster in discrete regions of theputP gene, suggesting that they may replace amino acids at the active site of the enzyme. IfputP substrate specificity mutations directly alter the active site of proline permease, the mutants should show specific defects in the kinetics of proline transport. In order to test this prediction, we examined the kinetics of threeputP substrate specificity mutants. One class of mutation increases theKm over 120-fold but only decreases theVmax fourfold. SuchKm mutants may be specifically defective in substrate recognition, thus identifying an amino acid critical for substrate binding. Another class of mutation decreases theVmax 80-fold without changing theKm.Vmax mutants appear to alter the rate of substrate translocation without affecting the substrate binding site. The last class of mutation alters both theKm andVmax of proline transport. These results indicate that substrate specificity mutations alter amino acids critical for Na+/proline symport. 相似文献
The carbon isotope composition of terrestrial C4 plants depends on the primary carboxylation of phosphoenolpyruvate (PEP) and on the diffusion of CO2 to the carboxylation sites, but is also influenced by the final carboxylation of ribulose-1,5-bisphosphate (RuBP). Several models have been used for reproducing this complex situation. In the present review, a particular model is applied as a means to interpret the effects of environmental and genetically determined factors on carbon isotope discrimination during C4 photosynthesis. As a new feature, the model considers four types of limitation of the overall CO2 assimilation rate. Both carboxylation reactions are assumed to be limited by either maximum enzyme activity or maximum substrate regeneration rate. The model is applied to experimental data on the effects of CO2, irradiance and water stress on short-term discrimination by leaves of several C4 species measured simultaneously with CO2 gas exchange characteristics. In particular, different patterns of the influence of low irradiances on carbon isotope discrimination are interpreted as due to variations in that irradiance at which a transition from limitation by PEP regeneration rate and RuBP carboxylase activity to limitation by the regeneration rates of both substrates occurs. After discussing literature data on the effects of environmental conditions on carbon isotope discrimination by C4 plants seasonal and developmental changes in carbon isotope composition, studies on the systematic and geographic distribution of C4 plants, evolutionary and genetical aspects, and some ecological implications are reviewed. 相似文献
The MARCKS (myristylated alanine-rich C-kinase substrate) protein is an abundant calmodulin-binding protein that is a major and specific endogenous substrate of protein kinase C (PKC). Stimulation of cells with phorbol esters or other activators of PKC has been shown previously to result in rapid phosphorylation of MARCKS proteins and redistribution of these myristylated C-kinase substrates from membrane to cytosol. Here we show that NIH3T3 murine fibroblasts transformed by p21-HA-C-RAS or pp60-V-SRC oncoproteins have markedly reduced levels of p68-MARCKS and that most of the remaining MARCKS protein is found in the cytosol. 3T3 cells containing a nontransforming oncoprotein p26-BCL2, in contrast, exhibited normal levels and distribution of p68-MARCKS. When taken together with recent evidence that MARCKS proteins are involved in regulating organization of the membrane cytoskeleton, our findings suggest that oncoprotein-mediated alterations in MARCKS protein levels and subcellular distribution may contribute to the development or maintenance of the transformed phenotpe. 相似文献
Chironomid pupal skins were collected during one year from three sites along a chalk stream in southern England. The sites had similar chemistry and discharge but differed in their current and temperature regimes. Substrate composition upstream of each collection point was surveyed during spring, summer and autumn. Proportions of macrophytes and sediments were compared with proportions of chironomid species and trophic groups. Seasonal changes in substrate and pupal skin collections were correlated by classification and direct ordination techniques. The distribution of chironomid species were indicated as being significantly related to the recorded substrate data. 相似文献
Macroinvertebrate density, biomass and drift were studied from moss-covered and moss-free channels in the South Fork Salmon River, Idaho. Insect densities were compared for 10 different substrate types and locations involving moss (Fontinalis neo-mexicana), sand, pebbles and cobbles. An ANOVA test demonstrated that insect densities varied significantly with substrate type (P < 0.05), and that total insect density in moss clumps differed significantly from densities in mineral substrates. Insect densities were 4–18 times greater in moss clumps than in mineral substrates under and adjacent to moss; sands under moss supported the lowest densities. During most tests, densities in pebble and cobble substrates adjacent to moss clumps were not significantly different from those found in similar substrates in the moss-free channel. The 20% moss-covered channel had 1.6 to 7.2 greater insect density and 1.4 to 6.1 greater biomass than did the moss-free channel for the tests conducted. Generally, midges (Chironomidae) made up over 50% of the insect community; annelids were the principal non-insect invertebrates.In spite of greater insect density and biomass in a moss-covered than in the moss-free channel, we did not demonstrate universally increased drift of the immature stages from the moss-covered channel, at least during daylight hours. As a consequence, we infer that salmonid fishes, feeding primarily on drifting insects during the daytime, may not derive increased caloric benefit from moss habitats until the insects emerge as adults. 相似文献
The degradation of phenol by Rhodococcus sp. P1 was studied in continuous culture systems. The organism could be adapted by slowly increasing concentration, step by step, up to 30.0 g · 1-1 phenol in the influent. The degradation rate reached values of about 0.3 g · g dry mass-1 ·h-1. Large step increases in phenol concentration and addition of further substrates (e.g., catechol) were tolerated up to a certain concentration. With increasing dilution rate and increasing inlet phenol concentration the stability of the system decreased. 相似文献