全文获取类型
收费全文 | 4206篇 |
免费 | 462篇 |
国内免费 | 345篇 |
出版年
2024年 | 12篇 |
2023年 | 96篇 |
2022年 | 77篇 |
2021年 | 138篇 |
2020年 | 174篇 |
2019年 | 208篇 |
2018年 | 181篇 |
2017年 | 187篇 |
2016年 | 179篇 |
2015年 | 171篇 |
2014年 | 225篇 |
2013年 | 269篇 |
2012年 | 181篇 |
2011年 | 176篇 |
2010年 | 151篇 |
2009年 | 199篇 |
2008年 | 203篇 |
2007年 | 205篇 |
2006年 | 220篇 |
2005年 | 191篇 |
2004年 | 186篇 |
2003年 | 121篇 |
2002年 | 108篇 |
2001年 | 90篇 |
2000年 | 94篇 |
1999年 | 98篇 |
1998年 | 77篇 |
1997年 | 64篇 |
1996年 | 66篇 |
1995年 | 56篇 |
1994年 | 67篇 |
1993年 | 57篇 |
1992年 | 60篇 |
1991年 | 55篇 |
1990年 | 44篇 |
1989年 | 42篇 |
1988年 | 24篇 |
1987年 | 24篇 |
1986年 | 22篇 |
1985年 | 37篇 |
1984年 | 30篇 |
1983年 | 11篇 |
1982年 | 25篇 |
1981年 | 20篇 |
1980年 | 19篇 |
1979年 | 19篇 |
1978年 | 16篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1973年 | 5篇 |
排序方式: 共有5013条查询结果,搜索用时 312 毫秒
71.
Karina Reiter Christoph Plutzar Dietmar Moser Philipp Semenchuk Karl-Heinz Erb Franz Essl Andreas Gattringer Helmut Haberl Fridolin Krausmann Bernd Lenzner Johannes Wessely Sarah Matej Robin Pouteau Stefan Dullinger 《Global Ecology and Biogeography》2023,32(6):855-866
Aim
Land use is the most pervasive driver of biodiversity loss. Predicting its impact on species richness (SR) is often based on indicators of habitat loss. However, the degradation of habitats, especially through land-use intensification, also affects species. Here, we evaluate whether an integrative metric of land-use intensity, the human appropriation of net primary production, is correlated with the decline of SR in used landscapes across the globe.Location
Global.Time period
Present.Major taxa studied
Birds, mammals and amphibians.Methods
Based on species range maps (spatial resolution: 20 km × 20 km) and an area-of-habitat approach, we calibrated a “species–energy model” by correlating the SR of three groups of vertebrates with net primary production and biogeographical covariables in “wilderness” areas (i.e., those where available energy is assumed to be still at pristine levels). We used this model to project the difference between pristine SR and the SR corresponding to the energy remaining in used landscapes (i.e., SR loss expected owing to human energy extraction outside wilderness areas). We validated the projected species loss by comparison with the realized and impending loss reconstructed from habitat conversion and documented by national Red Lists.Results
Species–energy models largely explained landscape-scale variation of mapped SR in wilderness areas (adjusted R2-values: 0.79–0.93). Model-based projections of SR loss were lower, on average, than reconstructed and documented ones, but the spatial patterns were correlated significantly, with stronger correlation in mammals (Pearson's r = 0.68) than in amphibians (r = 0.60) and birds (r = 0.57).Main conclusions
Our results suggest that the human appropriation of net primary production is a useful indicator of heterotrophic species loss in used landscapes, hence we recommend its inclusion in models based on species–area relationships to improve predictions of land-use-driven biodiversity loss. 相似文献72.
Aamir Hamid Khan Ling Min Yizan Ma Muhammad Zeeshan Shuangxia Jin Xianlong Zhang 《Plant biotechnology journal》2023,21(4):680-697
Global food security is one of the utmost essential challenges in the 21st century in providing enough food for the growing population while coping with the already stressed environment. High temperature (HT) is one of the main factors affecting plant growth, development and reproduction and causes male sterility in plants. In male reproductive tissues, metabolic changes induced by HT involve carbohydrates, lipids, hormones, epigenetics and reactive oxygen species, leading to male sterility and ultimately reducing yield. Understanding the mechanism and genes involved in these pathways during the HT stress response will provide a new path to improve crops by using molecular breeding and biotechnological approaches. Moreover, this review provides insight into male sterility and integrates this with suggested strategies to enhance crop tolerance under HT stress conditions at the reproductive stage. 相似文献
73.
74.
75.
Rosamarie Frieri William Fisher Rosenberger Nancy Flournoy Zhantao Lin 《Biometrics》2023,79(3):2565-2576
When there is a predictive biomarker, enrichment can focus the clinical trial on a benefiting subpopulation. We describe a two-stage enrichment design, in which the first stage is designed to efficiently estimate a threshold and the second stage is a “phase III-like” trial on the enriched population. The goal of this paper is to explore design issues: sample size in Stages 1 and 2, and re-estimation of the Stage 2 sample size following Stage 1. By treating these as separate trials, we can gain insight into how the predictive nature of the biomarker specifically impacts the sample size. We also show that failure to adequately estimate the threshold can have disastrous consequences in the second stage. While any bivariate model could be used, we assume a continuous outcome and continuous biomarker, described by a bivariate normal model. The correlation coefficient between the outcome and biomarker is the key to understanding the behavior of the design, both for predictive and prognostic biomarkers. Through a series of simulations we illustrate the impact of model misspecification, consequences of poor threshold estimation, and requisite sample sizes that depend on the predictive nature of the biomarker. Such insight should be helpful in understanding and designing enrichment trials. 相似文献
76.
Andrew J. Veselka Andrés Aponte-Gutiérrez Osmary A. Medina-Báez James I. Watling 《Biotropica》2023,55(2):540-550
Amphibians and reptiles are sensitive to changes in the thermal environment, which varies considerably in human-modified landscapes. Although it is known that thermal traits of species influence their distribution in modified landscapes, how herpetofauna respond specifically to shifts in ambient temperature along forest edges remains unclear. This may be because most studies focus on local-scale metrics of edge exposure, which only account for a single edge or habitat patch. We predicted that accounting for the combined effect of multiple habitat edges in a landscape would best explain herpetofaunal response to thermally mediated edge effects. We (1) surveyed herpetofauna at two lowland, fragmented forest sites in central Colombia, (2) measured the critical thermal maximum (CTmax) of the species sampled, (3) measured their edge exposure at both local and landscape scales, and (4) created a thermal profile of the landscape itself. We found that species with low CTmax occurred both further from forest edges and in areas of denser vegetation, but were unaffected by the landscape-scale configuration of habitat edges. Variation in the thermal landscape was driven primarily by changes in vegetation density. Our results suggest that amphibians and reptiles with low CTmax are limited by both canopy gaps and proximity to edge, making them especially vulnerable to human modification of tropical forest. Abstract in Spanish is available with online material. 相似文献
77.
Masatoshi Murayama Hirohito Hirata Makoto Shiraki Juan L. Iovanna Takayoshi Yamaza Toshio Kukita Toshihisa Komori Takeshi Moriishi Masaya Ueno Tadatsugu Morimoto Masaaki Mawatari Akiko Kukita 《Journal of cellular physiology》2023,238(3):566-581
Nuclear protein 1 (NUPR1) is a stress-induced protein activated by various stresses, such as inflammation and oxidative stress. We previously reported that Nupr1 deficiency increased bone volume by enhancing bone formation in 11-week-old mice. Analysis of differentially expressed genes between wild-type (WT) and Nupr1-knockout (Nupr1-KO) osteocytes revealed that high temperature requirement A 1 (HTRA1), a serine protease implicated in osteogenesis and transforming growth factor-β signaling was markedly downregulated in Nupr1-KO osteocytes. Nupr1 deficiency also markedly reduced HtrA1 expression, but enhanced SMAD1 signaling in in vitro-cultured primary osteoblasts. In contrast, Nupr1 overexpression enhanced HtrA1 expression in osteoblasts, suggesting that Nupr1 regulates HtrA1 expression, thereby suppressing osteoblastogenesis. Since HtrA1 is also involved in cellular senescence and age-related diseases, we analyzed aging-related bone loss in Nupr1-KO mice. Significant spine trabecular bone loss was noted in WT male and female mice during 6−19 months of age, whereas aging-related trabecular bone loss was attenuated, especially in Nupr1-KO male mice. Moreover, cellular senescence-related markers were upregulated in the osteocytes of 6−19-month-old WT male mice but markedly downregulated in the osteocytes of 19-month-old Nupr1-KO male mice. Oxidative stress-induced cellular senescence stimulated Nupr1 and HtrA1 expression in in vitro-cultured primary osteoblasts, and Nupr1 overexpression enhanced p16ink4a expression in osteoblasts. Finally, NUPR1 expression in osteocytes isolated from the bones of patients with osteoarthritis was correlated with age. Collectively, these results indicate that Nupr1 regulates HtrA1-mediated osteoblast differentiation and senescence. Our findings unveil a novel Nupr1/HtrA1 axis, which may play pivotal roles in bone formation and age-related bone loss. 相似文献
78.
Akifumi Makita Yasuo Konno Noboru Fujita Ken′ichi Takada Etsuji Hamabata 《Ecological Research》1993,8(2):215-224
The recovery process of aSasa tsuboiana population after a mass flowering and death in 1977 was investigated by 15 years of observation in the Hira Mountains, Kinki
district, western Japan. Seed production was high (6600–13 800 seeds m−2 inSasa plots and 3900 seeds m−2 in a forest plot) but emergent seedling density was low (14–21 seedlings m−2), probably because of seed predation byMicrotus montebelli occurring between seed shedding and the next spring. The seedling density had decreased further by the next year and theS. tsuboiana population recovered from only a limited number of seedlings. In spite of such a low initial density, theS. tsuboiana population was able to regenerate successfully and attained the previous full stand height in 7–16 years.Miscantbus sinensis invaded and delayed the recovery ofS. tsuboiana in one plot, butS. tsuboiana became dominant as it caught up with the height ofM. sinensis. Seedling growth patterns, such as frequent tillering, the onset of rhizome extension in the early stage of seedling growth
and frequent culm production from rhizomes, played important roles in the successful regeneration ofS. tsuboiana. 相似文献
79.
Akira Hikosaka Noriyuki Satoh Kazuhiro W. Makabe 《Development genes and evolution》1993,203(1-2):104-112
pHrMA4a-Z is a recombinant plasmid in which about 1.4 kb of the 5 flanking region of a gene for muscle actin HrMA4a from the ascidian Halocynthia roretzi is fused with the coding sequence of a bacterial gene for -galactosidase (lac-Z). In this study, we examined the expression of the fusion gene construct when it was introduced into eggs of another ascidian, namely Ciona savignyi. When a moderate amount of linearized pHrMA4a-Z was introduced into fertilized Ciona eggs, the expression of the reporter gene was evident in muscle cells of the larvae, suggesting that both species share a common machinery for the expression of muscle actin genes. The 5 upstream region of HrMA4a contains several consensus sequences, including a TATA box at -30, a CArG box at -116 and four E-boxes within a region of 200 bp. A deletion construct, in which only the 216-bp 5 flanking region of HrMA4a was fused with lac-Z, was expressed primarily in larval muscle cells. However, another deletion construct consisting of only the 61-bp upstream region of HrMA4a fused with lac-Z was not expressed at all. When pHrMA4a-Z or pHrMA4a-Z (–216) was injected into each of the muscle-precursor blastomeres of the 8-cell embryo, expression of the reporter gene was observed in larval muscle cells in a lineage-specific fashion. However, expression of the reporter gene was not observed when the plasmid was injected into non-muscle lineage. Therefore, the expression of the reporter gene may depend on some difference in cytoplasmic constituents between blastomeres of muscle and non-muscle lineage in the 8-cell embyo. 相似文献
80.
儿童最大有氧活动能力的发展特征 总被引:4,自引:1,他引:3
本文报告了我国463名10-19岁儿童青少年的最大有氧活动能力的发展特征。在青春早期,男女童的最大吸氧量绝对值均随年龄增长而增加,男童由1.75升/分增至3.10升/分,女童由1.44升/分增至2.07升/分,女童增长较少;以后女童即稳定于这一水平,男童仍略有增长。按身高及按最大心率计标的相对值与其有相似的特征。按体重和瘦体重计算的相对值,在男女童都未见随年龄增长的规律。男童VO2max绝对值及各 相似文献