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61.
Ecosystems of three trophic levels may be bottom-up (by food-plant availability) and/or top-down (by predators) limited. Top-down control might be of greater consequence when the predation impact comes from an alien predator. We conducted a replicated two-factor experiment with field voles (Microtus agrestis) during 2004-2005 on small islands of the outer archipelago of the Baltic Sea, south-west Finland, manipulating both predation impact by introduced American mink (Mustela vison) and winter food supply. In autumn 2004, we live-trapped voles on five islands from which mink had been consistently removed, and on four islands where mink were present, and provided half of these islands with 1.8 kg oats per vole. Body mass of female voles increased as a response to supplementary food, whereas both food supplementation and mink removal increased the body mass of male voles in subsequent spring. During winter, there was a positive effect of supplementary food, but in the subsequent summer, possible positive long-term impacts of food supplementation on field voles were not detected. Mink removal appeared not to affect density estimates of field voles during the winter and summer immediately after food addition. Trapping data from 2004 to 2005 and 2007 suggested, however, that in two out of three summers densities of voles were significantly higher in the absence than in the presence of mink. We conclude that vole populations on small islands in the archipelago of the Baltic Sea are mainly bottom-up limited during winter (outside the growing season of food plants), when food availability is low, and limited by mink predation during summer which slows population growth during the reproductive season of voles. 相似文献
62.
Tetraspanin CD151 associates with laminin-binding α(3)β(1)/α(6)β(1) integrins in epithelial cells and regulates adhesion-dependent signaling events. We found here that CD151 plays a role in recruiting Ras, Rac1, and Cdc42, but not Rho, to the cell membrane region, leading to the formation of α(3)β(1)/α(6)β(1) integrin-CD151-GTPases complexes. Furthermore, cell adhesion to laminin enhanced CD151 association with β(1) integrin and, thereby, increased complex formation between the β(1) family of integrins and small GTPases, Ras, Rac1, and Cdc42. Adhesion receptor complex-associated small GTPases were activated by CD151-β(1) integrin complex-stimulating adhesion events, such as α(3)β(1)/α(6)β(1) integrin-activating cell-to-laminin adhesion and homophilic CD151 interaction-generating cell-to-cell adhesion. Additionally, FAK and Src appeared to participate in this adhesion-dependent activation of small GTPases. However, engagement of laminin-binding integrins in CD151-deficient cells or CD151-specific siRNA-transfected cells did not activate these GTPases to the level of cells expressing CD151. Small GTPases activated by engagement of CD151-β(1) integrin complexes contributed to CD151-induced cell motility and MMP-9 expression in human melanoma cells. Importantly, among the four tetraspanin proteins that associate with β(1) integrin, only CD151 exhibited the ability to facilitate complex formation between the β(1) family of integrins and small GTPases and stimulate β(1) integrin-dependent activation of small GTPases. These results suggest that CD151 links α(3)β(1)/α(6)β(1) integrins to Ras, Rac1, and Cdc42 by promoting the formation of multimolecular complexes in the membrane, which leads to the up-regulation of adhesion-dependent small GTPase activation. 相似文献
63.
Pierangela Giustetto Miriam Filippi Mauro Castano Enzo Terreno 《Journal of visualized experiments : JoVE》2015,(97)
Photoacoustics and high frequency ultrasound stands out as powerful tools for neurobiological applications enabling high-resolution imaging on the central nervous system of small animals. However, transdermal and transcranial neuroimaging is frequently affected by low sensitivity, image aberrations and loss of space resolution, requiring scalp or even skull removal before imaging. To overcome this challenge, a new protocol is presented to gain significant insights in brain hemodynamics by photoacoustic and high-frequency ultrasounds imaging with the animal skin and skull intact. The procedure relies on the passage of ultrasound (US) waves and laser directly through the fissures that are naturally present on the animal cranium. By juxtaposing the imaging transducer device exactly in correspondence to these selected areas where the skull has a reduced thickness or is totally absent, one can acquire high quality deep images and explore internal brain regions that are usually difficult to anatomically or functionally describe without an invasive approach. By applying this experimental procedure, significant data can be collected in both sonic and optoacoustic modalities, enabling to image the parenchymal and the vascular anatomy far below the head surface. Deep brain features such as parenchymal convolutions and fissures separating the lobes were clearly visible. Moreover, the configuration of large and small blood vessels was imaged at several millimeters of depth, and precise information were collected about blood fluxes, vascular stream velocities and the hemoglobin chemical state. This repertoire of data could be crucial in several research contests, ranging from brain vascular disease studies to experimental techniques involving the systemic administration of exogenous chemicals or other objects endowed with imaging contrast enhancement properties. In conclusion, thanks to the presented protocol, the US and PA techniques become an attractive noninvasive performance-competitive means for cortical and internal brain imaging, retaining a significant potential in many neurologic fields. 相似文献
64.
目的探讨亲缘供者外周血红细胞参数对COBE Spectra血细胞分离机的自动外周血干细胞采集程序(AutoPBSC程序)与单个核细胞采集程序(MNC程序)的影响及经验分析。 方法选取河北燕达陆道培医院2019年6月至2021年2月小红细胞亲缘供者31例45次采集为小红细胞组,选取同期非小红细胞亲缘供者51例60次采集为非小红细胞组,分别应用AutoPBSC程序和MNC程序,比较两组采集情况及采集产品相关指标。采用独立样本t检验和Mann-Whitney U检验分析2组计量资料的差异。 结果与小红细胞AutoPBSC程序组比较,小红细胞MNC程序组血小板(PLT)降低率[(25.88±15.83)﹪比(36.64±10.22)﹪]、采集效率[32.65﹪(23.60﹪,73.82﹪)比63.74﹪ (59.83﹪,68.55﹪)]、采集物体积[(158.83±34.39)比(222.91±63.9)mL]、MNC总数[(218.04±117.57)×108/L比(350.24±127.64)×108/L]、CD34+细胞总数[113.83×106/L (79.25×106/L,154.10×106/L)比233.26×106/L (177.18×106/L,392.51×106/L)]、MNC计数[(4.04±2.61)×108/kg比(5.54±2.22)×108/ kg]、CD34+计数[1.84×106/kg (1.16×106/kg,4.41×106/kg)比3.64×106/kg (2.49×106/kg,6.37×106/kg)]均升高,差异有统计学意义(P < 0.05);与非小红细胞AutoPBSC程序组比较,非小红细胞组MNC程序组采集物体积[(162.83±51.74)比(242.56±43.25)mL]升高,差异有统计学意义(P < 0.05)。 结论对造血干细胞移植供者红细胞体积偏小时,应用MNC程序采集外周血造血干细胞,比应用AutoPBSC程序更有优势。 相似文献
65.
植物功能性状可以反映植物应对环境变化的适应策略。本文以黄梅秤锤树(Sinojackia huangmeiensis)当前唯一野生种群为对象, 比较了3种微生境(湖边、种群中心、耕地边)中该物种的叶片功能性状均值、种内变异和叶片生态化学计量特征的差异, 分析了黄梅秤锤树对湖岸带微生境变化的响应及其适应策略。结果表明: (1) 3种微生境中土壤C、N、P含量没有显著性差异(P > 0.05), 但土壤C∶N和C∶P具有显著性差异(P < 0.05), 土壤类型和养分条件有所不同。(2)黄梅秤锤树叶片功能性状的比较用单因素方差分析和贝叶斯方差分析得出的结果一致, 均为叶长、叶面积和比叶面积在中心区域显著高于湖边(P < 0.05), 而耕地边与湖边和中心区域均没有显著差异(P > 0.05); 叶N含量在湖边显著高于中心区域和耕地边(P < 0.05), 而中心区域和耕地边间没有显著差异(P > 0.05); 叶宽、叶长/叶宽、叶干物质含量、叶C和叶P含量在3种微生境间都没有显著性差异(P > 0.05)。(3)黄梅秤锤树叶片的N∶P在湖边显著高于中心区域和耕地边(P < 0.05), C∶N在湖边显著小于中心区域和耕地边(P < 0.05), N∶P和C∶N在中心区域和耕地边没有显著性差异(P > 0.05), C∶P在3种微生境间都没有显著性差异(P > 0.05)。(4)黄梅秤锤树叶片功能性状的总体变异程度在0.02-0.28之间, 其中叶片C和N含量在湖边和中心区域的种内变异程度显著较低, 表明3种生境中湖边和中心区域黄梅秤锤树种群的稳定性相对较差。(5)湖边黄梅秤锤树主要通过增加叶N含量促进生长; 中心区域黄梅秤锤树主要通过增加叶面积和比叶面积以及提高叶N的利用效率来提高光捕获能力促进生长; 耕地边黄梅秤锤树的叶N含量和叶面积、比叶面积都处于中等水平, 通过性状之间的共同作用使植株生长达到最佳水平。以上结果表明, 由于微地形、水位波动和土壤环境条件的差异, 黄梅秤锤树对3种生境中的适应策略有所不同, 并且不是通过单一性状调整来适应环境的变化, 而是通过多种性状之间的权衡达到更好的适应效果。 相似文献
66.
“氯敌鼠”防治对农田小哺乳动物群落结构的影响及群落演替分析 总被引:3,自引:0,他引:3
对不同防治对策下施用氯敌鼠作为杀鼠剂对农田小哺乳动物群落结构的影响进行了研究.结果表明,杀鼠剂对群落组成有很大影响,灭鼠时机、灭鼠次数不同,群落的反应也不同.秋季灭鼠,小哺乳动物数量逐年下降,3 年后可达到较低密度.群落多样性也逐年下跌,且回升极弱.春季灭鼠,数量可持续在低水平,群落多样性较其它处理高,对化学灭鼠破坏的生物多样性有较强的恢复力.灭鼠频次的增加对小哺乳动物总体数量降低有强化作用.可以认为,化学灭鼠明显地降低了群落中小哺乳动物的密度,也降低了群落的多样性.促使小哺乳动物群落向着优势种突出、种类单调、群落稳定性差的方向演替. 相似文献
67.
大口鲇和鲇鱼血清蛋白质及同工酶的比较研究 总被引:10,自引:0,他引:10
采用聚丙烯酰胺梯度凝胶垂直板电泳,分析了大口鲇和鲇鱼的血清蛋白质以及心脏、肝脏、眼和肌肉4种组织的EST及MDH同工酶。结果表明,大口鲇和鲇鱼的血清蛋白质均能分离出20条左右的谱带,两者既表现出相同的谱带,又表现出迁移率和含量都不同的带型。两者的EST和MDH同工酶在4种组织及血清中均能特异性地表达,存在明显的组织和物种特异性。本文认为肝脏是研究大口鲇和鲇鱼种群生化遗传结构与变异的理想材料,同时还探讨了两种鲇鱼的M DH同工酶位点。
Abstract:The serum proteins and isozymes in four tissues (heart,liver,eye and musele)of Smeridionalis Chen and S.asotus Linnaeus were analyzed by polyacrylamide gradient gel vertical electrophoresis.The isozymes are esterase(EST)and malate dehytrogenase(MDH).The results showed that electrophoretograme of serum proteins were about 20 protein pattens in two species catfish,they were either the same protein pattens or the different pattens.Electrophoretogram of isozymes(EST,MDH)in two species catfish indicated tissues and species specificity.Experiment considered that the liver was a good material studied biochemical genetic constitution and variation in species group of S.meridionalis Chen and S.asotus Linnaeus. 相似文献
68.
Asano R Ikoma K Shimomura I Taki S Nakanishi T Umetsu M Kumagai I 《The Journal of biological chemistry》2011,286(3):1812-1818
Diabodies (Dbs) and tandem single-chain variable fragments (taFv) are the most widely used recombinant formats for constructing small bispecific antibodies. However, only a few studies have compared these formats, and none have discussed their binding kinetics and cross-linking ability. We previously reported the usefulness for cancer immunotherapy of a humanized bispecific Db (hEx3-Db) and its single-chain format (hEx3-scDb) that target epidermal growth factor receptor and CD3. Here, we converted hEx3-Db into a taFv format to investigate how format affects the function of a small bispecific antibody; our investigation included a cytotoxicity assay, surface plasmon resonance spectroscopy, thermodynamic analysis, and flow cytometry. The prepared taFv (hEx3-taFv) showed an enhanced cytotoxicity, which may be attributable to a structural superiority to the diabody format in cross-linking target cells but not to differences in the binding affinities of the formats. Comparable cross-linking ability for soluble antigens was observed among hEx3-Db, hEx3-scDb, and hEx3-taFv with surface plasmon resonance spectroscopy. Furthermore, drastic increases in cytotoxicity were found in the dimeric form of hEx3-taFv, especially when the two hEx3-taFv were covalently linked. Our results show that converting the format of small bispecific antibodies can improve their function. In particular, for small bispecific antibodies that target tumor and immune cells, a functional orientation that avoids steric hindrance in cross-linking two target cells may be important in enhancing the growth inhibition effect. 相似文献
69.
环境温度的变化影响野生啮齿动物的消化道形态与功能。小肠是吸收营养成分的主要部位,其结构和功能具有可塑性。为了解小肠黏膜的结构和功能对环境温度变化的响应机制,以布氏田鼠为研究对象,比较了低温组和常温组动物小肠黏膜的组织结构和小肠黏膜免疫相关细胞的数目。结果显示:(1)低温组布氏田鼠的十二指肠、空肠和回肠的绒毛长度及绒毛长度与隐窝深度的比值均高于对照组;(2)低温驯化使布氏田鼠小肠上皮内淋巴细胞的数量增加;(3)低温驯化使布氏田鼠十二指肠、空肠和回肠的杯状细胞数量均显著增加。结果表明,在低温环境下布氏田鼠的小肠黏膜结构和免疫细胞的数量发生了可塑性变化,这可能与低温环境下的高能量需求和免疫功能的变化有关。 相似文献
70.
Il Sang Yoon 《Analytical biochemistry》2010,407(2):205-210
Parkinson’s disease (PD) is a neurodegenerative disease featured by selective loss of substantia nigra neurons. Rotenone administration in animals induces neurodegeneration accompanied by α-synuclein-positive Lewy body-like inclusions, recapturing typical histopathological features of PD. In an effort to screen for small-molecule agents to reverse rotenone-induced cytotoxicity, we developed and validated a sensitive and robust assay with neuroblastoma SK-N-SH cells. This assay was amenable to a high-throughput screening format with Z′ factor of 0.56. Robotic screening of a bioactive compound library led to the identification of carnosic acid that can effectively protect cells from rotenone treatment. Using a high-content image-based assay and Western blot analysis, we demonstrated that carnosic acid protects cells from rotenone stress by significant induction of HSP70 expression. Therefore, the assay reported here can be used to identify novel cytoprotective agents for clinical therapeutics of PD. 相似文献