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141.
Milad Radiom Yong He Juan Peng-Wang Armelle Baeza-Squiban Jean-François Berret Yong Chen 《Biotechnology and bioengineering》2020,117(9):2827-2841
We report on the development of a new model of alveolar air–tissue interface on a chip. The model consists of an array of suspended hexagonal monolayers of gelatin nanofibers supported by microframes and a microfluidic device for the patch integration. The suspended monolayers are deformed to a central displacement of 40–80 µm at the air–liquid interface by application of air pressure in the range of 200–1,000 Pa. With respect to the diameter of the monolayers, that is, 500 µm, this displacement corresponds to a linear strain of 2–10% in agreement with the physiological strain range in the lung alveoli. The culture of A549 cells on the monolayers for an incubation time of 1–3 days showed viability in the model. We exerted a periodic strain of 5% at a frequency of 0.2 Hz for 1 hr to the cells. We found that the cells were strongly coupled to the nanofibers, but the strain reduced the coupling and induced remodeling of the actin cytoskeleton, which led to a better tissue formation. Our model can serve as a versatile tool in lung investigations such as in inhalation toxicology and therapy. 相似文献
142.
Christina Bergonzo Kunal Dharmadhikari Emily Samuels Makenzie Christensen Jennifer Tullman 《Proteins》2020,88(9):1189-1196
ClpS2 is a small protein under development as a probe for selectively recognizing N-terminal amino acids of N-degron peptide fragments. To understand the structural basis of ClpS2 specificity for an N-terminal amino acid, all atom molecular dynamics (MD) simulations were conducted using the sequence of a bench-stable mutant of ClpS2, called PROSS. We predicted that a single amino acid leucine to asparagine substitution would switch the specificity of PROSS ClpS2 to an N-terminal tyrosine over the preferred phenylalanine. Experimental validation of the mutant using a fluorescent yeast-display assay showed an increase in tyrosine binding over phenylalanine, in support of the proposed hypothesis. 相似文献
143.
144.
Toward Long‐Term Stable and Highly Efficient Perovskite Solar Cells via Effective Charge Transporting Materials 下载免费PDF全文
Perovskite solar cells (PSCs) have advanced quickly with their power conversion efficiency approaching the record of silicon solar cells. However, there is still a big challenge to obtain both high efficiency and long‐term stability for future commercialization of PSCs. The major instability issue is associated with the decomposition or phase transition of perovskite materials that are believed to be intrinsically unstable under outdoor working conditions. Herein, the authors review the approaches that marked important progress in developing new functional electron/hole transporting materials that enabled highly efficient and stable PSCs. The findings that accelerate charge diffusion and that suppress the irrevocable loss of ions diffusing out of perovskite materials and other diffusion processes are highlighted. In addition, derivative interface engineering methods to control the diffusion process of charges/ions/molecules are also reviewed. Finally, the authors propose key research issues in charge transporting materials and interface engineering with regard to the important diffusion processes that will be one of the keys to realize highly efficient and long‐term stable PSCs. 相似文献
145.
146.
Qi Li Yao Zhao Wenke Zhou Zhengyuan Han Rui Fu Fang Lin Dapeng Yu Qing Zhao 《Liver Transplantation》2019,9(46)
The performance of perovskite solar cells (PSCs) relies on the synthesis method and chemical composition of the perovskite materials. So far, PSCs that have adopted two‐step sequential deposited perovskite with the state‐of‐art composition (FAPbI3)1?x(MAPbBr3)x (x < 0.05) have achieved record power conversion efficiency (PCE), while their one‐step antisolvent dripping counterparts with typical composition Cs0.05FA0.81MA0.14Pb(I0.85Br0.15)3 with more bromine have exhibited much better long‐term operational stability. Thus, halogen engineering that aims to elevate bromine content in sequential deposited perovskite film would push operational stability of PSCs toward that of antisolvent dripping deposited perovskite materials. Here, a Br‐rich seeding growth method is devised and perovskite seed solution with high bromine content is introduced into a PbI2 precursor, leading to bromine incorporation in the resulting perovskite film. Photovoltaic devices fabricated by Br‐rich seeding growth method exhibit a PCE of 21.5%, similar to 21.6% for PSCs having lower bromine content. Whereas, the operational stability of PSCs with higher bromine content is significantly enhanced, with over 80% of initial PCE retained after 500 h tracking at maximum power point under 1‐sun illumination. This work highlights the vital importance of halogen composition for the operational stability of PSCs, and introduces an effective way to incorporate bromine into mixed‐cation‐halide perovskite film via sequential deposition method. 相似文献
147.
148.
Summary Presented here are techniques developed to culture and analyze three-dimensional (3-D) adipose-like tissues as a means to
bridge the gap between current liminations in culturing preadipocytes (PAs) and that of providing clinically relevant volumes
of adipose tissue useful for soft tissue engineering stratgies in reconstructive surgery. Pilot studies were performed to
determine techniques to visualize and analyze 3-D PA-like tissues as well as to develop successful strategies to culture 3T3-L1
cells in a high aspect ratio vessel rotating-wall bioreactor both with and without microcarriers. Next, a series of cultures
were accessed to verify these techniques as well as to compare the culture of the cells with and without microcarriers. Finally,
a perfused rotating-wall bioreactor was used to further investigate the nature of the aggregates or tissues being generated.
The aggregates that formed in the perfused system were analyzed via histology and in vivo animal studies. PA-like tissues
as large as 4–5 mm in diameter without microcarriers that were capable of lipid-loading and composed of viable cells were
achieved. We have successfully demonstrated that large tissue aggregates can be grown in bioreactor culture systems. 相似文献
149.
James S. Wakeley Michael P. Guilfoyle Terry J. Antrobus Richard A. Fischer Wylie C. BarrowJr. Paul B. Hamel 《Wetlands Ecology and Management》2007,15(5):417-439
We used an ordination approach to identify factors important to the organization of breeding bird communities in three floodplains:
Cache River, Arkansas (AR), Iatt Creek, Louisiana (LA), and the Coosawhatchie River, South Carolina (SC), USA. We used 5-min
point counts to sample birds in each study area each spring from 1995 to 1998, and measured ground-surface elevations and
a suite of other habitat variables to investigate bird distributions and community characteristics in relation to important
environmental gradients. In both AR and SC, the average number of Neotropical migrant species detected was lowest in semipermanently
flooded Nyssa aquatica Linnaeus habitats and greatest in the highest elevation floodplain zone. Melanerpes carolinus Linnaeus, Protonotaria citrea Boddaert, Quiscalus quiscula Linnaeus, and other species were more abundant in N. aquatica habitats, whereas Wilsonia citrina Boddaert, Oporornis formosus Wilson, Vireo griseus Boddaert, and others were more abundant in drier floodplain zones. In LA, there were no significant differences in community
metrics or bird species abundances among forest types. Canonical correspondence analyses revealed that structural development
of understory vegetation was the most important factor affecting bird distributions in all three study areas; however, potential
causes of these structural gradients differed. In AR and SC, differences in habitat structure were related to the hydrologic
gradient, as indexed by ground-surface elevation. In LA, structural variations were related mainly to the frequency of canopy
gaps. Thus, bird communities in all three areas appeared to be organized primarily in response to repeated localized disturbance.
Our results suggest that regular disturbance due to flooding plays an important role in structuring breeding bird communities
in floodplains subject to prolonged inundation, whereas other agents of disturbance (e.g., canopy gaps) may be more important
in headwater systems subject to only short-duration flooding. Management for avian community integrity in these systems should
strive to maintain forest zonation and natural disturbance regimes. 相似文献
150.
Drewitz M Helbling M Fried N Bieri M Moritz W Lichtenberg J Kelm JM 《Biotechnology journal》2011,6(12):1488-1496
Although the relevance of three-dimensional (3-D) culture has been recognized for years and exploited at an academic level, its translation to industrial applications has been slow. The development of reliable high-throughput technologies is clearly a prerequisite for the industrial implementation of 3-D models. In this study the robustness of spherical microtissue production and drug testing in a 96-well hanging-drop multiwell plate format was assessed on a standard 96-well channel robotic platform. Microtissue models derived from six different cell lines were produced and characterized according to their growth profile and morphology displaying high-density tissue-like reformation and growth over at least 15 days. The colon cancer cell line HCT116 was chosen as a model to assess microtissue-based assay reproducibility. Within three individual production batches the size variations of the produced microtissues were below 5%. Reliability of the microtissue-based assay was tested using two reference compounds, staurosporine and chlorambucil. In four independent drug testings the calculated IC(50) values were benchmarked against 2-D multiwell testings displaying similar consistency. The technology presented here for the automated production of a variety of microtissues for efficacy testing in a standard 96-well format will aid the implementation of more organotypic models at an early time point in the drug discovery process. 相似文献