Partial purification from hepatoma cells of an intracellular substance which mediates the effects of insulin on pyruvate dehydrogenase and low Km cyclic AMP phosphodiesterase

A substance capable of stimulating the activities of pyruvate dehydrogenase and low K_m cyclic AMP phosphodiesterase was prepared from H4-II-EC3′ hepatoma cells by acid extraction and partially purified by molecular exclusion chromatography. The material thus prepared by gel chromatography was found to stimulate the activities of these enzymes in a concentration-dependent manner. The amount or activity of the pyruvate dehydrogenase stimulating factor was increased in cells which had been treated with physiological concentrations of insulin (0.2 mU/ml). Increasing the concentration of insulin increased the amount or activity of the factor generated. High concentrations of insulin did not cause a reversal of the effects of insulin. The stimulation of pyruvate dehydrogenase activity by the factor was eliminated when sodium fluoride (75 mm) was present in the enzyme assay, implying that activation was mediated by the pyruvate dehydrogenase phosphatase. The enzyme-stimulating factor isolated from hepatoma cells shares a number of important characteristics with the putative second messenger of insulin prepared from other cell types: (1) it is heat and acid stable, (2) it has a similar apparent molecular weight, (3) it is generated in an insulin-dependent manner, (4) it stimulates the activity of pyruvate dehydrogenase by a fluoride-sensitive mechanism, and (5) it elutes from the anion-exchange resin AG 1-X8 at an ionic strength of 0.4 m. These findings suggest that the stimulator of pyruvate dehydrogenase and of low K_m cyclic AMP phosphodiesterase isolated from hepatoma cells has chemical properties identical with those of the putative second messenger of insulin action isolated from a number of other insulin-sensitive tissues.     

Dendroclimatic signals in the pine and spruce chronologies in the Solovetsky Archipelago

Searching for a robust tree-ring parameter useful for paleoclimatic purposes is one of the most demanding topics in the modern paleoscience. Since Blue Intensity has already expressed itself in different geographical locations all over the world as a possible replacement for maximum density, close attention is paid to investigate features of the inferred signal. The Solovki Islands is a unique location in Northern Russia where two important factors that make this territory attractive for developing a long tree-ring chronology have been met: modern long-living trees and building activities using old trees that were started by monks in the middle of the 16th century. The main goal of the research is to develop pine and spruce chronologies based on tree-ring width (TRW) and delta Blue Intensity (dBI) and to assess the ability of these parameters to be used as climate predictors. As a result, 14 conifer chronologies from 7 sites (4 for pine and 3 for spruce) were developed. The composite pine and spruce chronologies span a period of 474 and 378 years each. Cross-correlation of dBI-based chronologies of both conifers is high (r = up to 0.71 while for TRW-based chronologies it is lower on average (−0.18 to 0.63). Intra-species correlation of TRW chronologies in some cases achieved even negative values (r = −0.18. Discrepancies found between TRW chronologies of pine and spruce could be explained by differences in climatic signals. Response function analysis with monthly temperatures revealed that growth of pine depends on the previous August, while spruce has a temporally stable and strong relation to June temperatures. Compared to TRW, dBI-based chronologies have a high correlation with summer temperatures (r = 0.64 and 0.66 for spruce and pine, respectively). Presented research points out the importance of the response function analysis suggesting that depending on goals of the study several tree-ring parameters could be used, e.g., tree-ring width of spruce responses to June temperatures, while dBI to the whole summer.     

Recognition and avoidance of insecticide-treated Scots Pine (Pinus sylvestris) by Hylobius abietis (Coleoptera: Curculionidae): implications for pest management strategies

Abstract 1 The feeding preferences of Hylobius abietis (L.) were studied in a series of choice and no‐choice trials for insecticide‐treated food, time‐to‐death studies and arena trials. 2 Treatment of Scots Pine twigs with a pyrethroid insecticide, lambda‐cyhalothrin, was compared with twigs treated with imidacloprid, a neonicotinoid. 3 Clear avoidance of insecticide‐treated food sources, with strong evidence of selection for untreated food sources, was shown. 4 In addition, it took up to 3 weeks for H. abietis to die from insecticide poisoning when fed on treated food and, during this time, it was potentially capable of finding new untreated food sources.     

Isolation of myelin basic protein-reactive T-cell lines from normal human blood

T-Cell lines which responded by proliferation to the autoantigen, myelin basic protein (MBP), were isolated from the blood of six of nine normal humans. These T-cell lines could be maintained in in vitro culture for up to 2 months through the use of Interleukin 2 and repeated MBP stimulation. Optimal antigen-induced proliferation required both antigen and antigen-presenting cells found in the adherent cell population of autologous peripheral blood mononuclear cells (PBM). The T-cell lines were predominantly of the helper phenotype (OKT3+, OKT4+, OKT8-) and responded to both human and guinea pig myelin basic protein.     

Modelling relation of first shoot emergence in various tree species to meteorological parameters in Slovenia

The relation of first shoot emergence in various tree species (birch, beech, oak and spruce) to meteorological parameters was studied on the basis of phenological and meteorological observations at five locations in Slovenia during 1967–1986. A physical model was developed using tree branch temperature obtained by the energy balance equation. The gained linear dependence of first shoot emergence on effective tree branch temperature was compared with the results obtained by multiple regression analysis among first shoot emergence, effective temperature, global radiation, wind velocity and precipitation. A new method was developed to define the proper biological temperature threshold which was used for effective temperature calculations. Results of the physical model and of the multiple regression analysis are statistically significant and give similar correlations between first shoot emergence and meteorological parameters.