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601.
602.
Tareerat Lertwimol Pacharapan Sonthithai Weerawan Hankamolsiri Pakkanun Kaewkong Paweena Uppanan 《Biotechnology progress》2023,39(2):e3322
Alginate hydrogel is an attractive biomaterial for cell microencapsulation. The microarchitecture of hydrogels can regulate cellular functions. This study aims to investigate the applicability of sodium citrate buffer (SCB) as a culture medium supplement for modulating the microstructure of alginate microbeads to provide a favorable microenvironment for chondrogenic induction. The chondrocyte-laden microbeads, with and without TGF-β3 incorporation, were produced through an encapsulator. The obtained small-sized microbeads (~300 μm) were exposed to a treatment medium containing SCB, composed of varied concentrations of sodium citrate (1.10–1.57 mM), sodium chloride (3.00–4.29 mM), and ethylenediaminetetraacetic acid (0.60–0.86 mM) to partially degrade their crosslinked structure for 3 days, followed by culture in a normal medium until day 21. Scanning electron microscope micrographs demonstrated a loose hydrogel network with an enhanced pore size in the SCB-treated microbeads. Increasing the concentration of SCB in the treatment medium reduced the calcium content of the microbeads via a Na+/Ca2+ exchange process and improved the water absorption of the microbeads, resulting in a higher swelling ratio. All the tested SCB concentrations were non-cytotoxic. Increases in aggrecan and type II collagen gene expression and their corresponding extracellular matrix accumulation, glycosaminoglycans, and type II collagen were vividly detected in the TGF-β3-containing microbeads with increasing SCB concentrations in the treatment medium. Our findings highlighted that the combination of SCB treatment and TGF-β3 incorporation in the chondrocyte-laden microbeads is a promising strategy for enhancing cartilage regeneration, which may contribute to a versatile application in cell delivery and tissue engineering. 相似文献
603.
Amanda Reges de Sena Flávio Manoel Barros Oliveira Tonny Cley Campos Leite Talita Camila Evaristo da Silva Nascimento Keila Aparecida Moreira 《Preparative biochemistry & biotechnology》2017,47(9):945-951
The aims of the current study are to assess the influence of polyethylene glycol (PEG) concentration, molar mass, pH, and citrate concentrations on aqueous biphasic systems based on 24 factorial designs, as well as to check their capacity to purify tannase secreted by Aspergillus tamarii URM 7115. Tannase was produced through submerged fermentation at 26°C for 67?h in Czapeck-Dox modified broth and added with yeast extract and tannic acid. The factorial design was followed to assess the influence of PEG molar mass (MPEG 600; 4,000 and 8,000?g/?mol), and PEG (CPEG 20.0; 22.0 and 24.0% w/w) and citrate concentrations (CCIT 15.0, 17.5, and 20.0%, w/w), as well as of pH (6.0, 7.0, and 8.0) on the response variables; moreover, partition coefficient (K), yield (Y), and purification factor (PF) were analyzed. The most suitable parameters to purify tannase secreted by A. tamarii URM 7115 through a biphasic system were 600 (g/mol) MPEG, 24% (w/w) CPEG, 15% (w/w) CCIT at pH 6.0 and they resulted in 6.33 enzyme partition, 131.25% yield, 19.80 purification factor and 195.08 selectivity. Tannase secreted by A. tamarii URM 7115 purified through aqueous biphasic systems composed of PEG/citrate can be used for industrial purposes, since it presents suitable purification factor and yield. 相似文献
604.
605.
I H Higuti M Stencel K H Nascimento A J Nascimento 《Cell biochemistry and function》1992,10(4):237-241
The membrane ATPase (EC 3.6.1.3) of Bacillus cereus was solubilized by a 'shock-wash' process and purified. The non-specific phosphatase contaminant was separated by glycerol density gradient centrifugation. The optimum temperature was 39.5 degrees C and the pH optimum at 7.5. On SDS-polyacrylamide gel electrophoresis two classes of subunits were observed in equal proportions with molecular weights of 70 K and 83 K. The effect of various compounds on the enzymatic activity was studied. The enzyme was insensitive to NaN3, oligomycin and to divalent cations, but was inhibited by citrate and oxalate. 相似文献
606.
Bicarbonate has been regarded as a major factor for inducing Zn deficiency in lowland rice, but the mechanisms responsible
for this effect are not yet fully understood. The objective of the present study was to test whether early effects of bicarbonate
(HCO3
−)are inhibition of root growth due to the accumulation of organic acids induced by HCO3
−. Solution culture experiments were conducted using two rice cultivars differing in susceptibility to Zn deficiency, and four
bicarbonate concentrations (0, 5, 10, 20 mM). Bicarbonate (5–20 mM) strongly inhibited root growth of the Zn-inefficient cultivar within 4 days of treatments. In contrast, root growth of the
Zn-efficient cultivar was slightly stimulated with bicarbonate at 5–10 mM and not affected at 20 mM. The inhibitory effect of bicarbonate on root growth in the Zn-inefficient cultivar was mainly that of impairment of new
root initiation rather than suppression of elongation of individual roots. Bicarbonate (5–20 mM) increased the concentrations of malate, succinate and citrate in the roots of both cultivars, but to a greater extent for
the Zn-inefficient than for the Zn-efficient cultivars. The results suggest that the impairment of root growth was likely
to be the initial action of bicarbonate in inducing Zn deficiency in lowland rice, and the inhibitory effect of bicarbonate
on root growth of the Zn-inefficient cultivar might result from high accumulation and an insufficient compartmentation of
organic acids in the root cells. 相似文献
607.