Chronobiologic Evaluation of Drug Efficacy in Hypertension

Time-associated variations of blood pressure became accesible due to the modern development of pressure monitoring technology. The data collection and analysis should be standardized and formulated. Time-associated changes of pressure were evaluated not only by nonparametric and conventional but also by parametric and rhythmometric estimation. The reference data (chronodesms) are essential to define the deviant magnitude of pressure. This chronobiologic approach became feasible to quantitate the efficacy of antihypertensive agents in hypertensives.     

不同产地与类型及采收方法对黄花蒿中青蒿素含量的影响

以来源于重庆、广西、湖南、广东、江苏、陕西等省区的72分种质资源为试验材料,研究不同产地与类型及采收方法对黄花蒿中青蒿素含量的影响,为良种的选育和药材的采收提供科学的依据。结果表明:(1)早熟型与中、晚熟型青蒿素含量的差异显著,其顺序为:中熟型>晚熟型>早熟型;(2)在同一管理条件下不同茎秆颜色的青蒿素含量之间差异显著,其顺序为:白杆型>黄绿秆型>紫秆型>绿秆型;(3)不同产地的黄花蒿青蒿素含量动态变化规律一致,青蒿素含量最高时期为孕蕾期,是最佳采收期;(4)不同部位之间青蒿素含量差异显著,以叶片的青蒿素含量最高。     

Human genetic databanks in Australia: indications of inconsistency and confusion

This paper reports on a survey of human biotechnology organizations in Australia. The study provides insights into the nature, use and practices involved with human genetic databanking in the country. The survey was conducted at a time when databanks were becoming increasingly important to an expanding genomics industry, and while the nature and extent of industry regulation was being debated. The data revealed a surprising level of confusion and inconsistency in the interpretation of terminology and in ethical practice, even among those organizations subject to the relevant government ethics guidelines. It is argued that despite the extensive level of public consultation, recommendations for reform and actual reform in the intervening years, human genetic databanking remains an under-regulated sector of the human biotechnology industry in Australia, and at least as far as the private sector is concerned, will remain so in the foreseeable future.     

一种烟粉虱成虫唾液酶鉴定与活性分析的方法

以B型烟粉虱Bemisia tabaci(Gennadius)成虫为材料,介绍了一种微型刺吸式昆虫唾液酶鉴定和分析的方法,主要包括人工饲养、唾液收集、唾液多酚氧化酶(PPO)和过氧化物酶(POD)的鉴定与活性分析。结果显示,B型烟粉虱在特异性嗜好寄主甘蓝上分泌的多酚氧化酶与过氧化物酶的比活力分别为嗜好寄主番茄上的1.54和1.65倍。该方法操作简捷,鉴定结果直观清晰,酶活测定灵敏,适合于其他微型刺吸式昆虫如蚜虫、木虱等的唾液酶研究。     

小麦微核心种质γ射线辐射敏感性分析

以259个小麦微核心种质为材料进行剂量为0、100、150、250 Gy的60Coγ射线辐照处理,探讨小麦微核心种质的γ射线辐射敏感性分布,以及DNA损伤修复基因TaKu70和TaKu80对辐照的应答模式。结果表明,小麦微核心种质的苗高损伤率与γ射线辐照剂量间存在着3种函数关系:对数、线性、幂函数。以苗高损伤率为50%时的辐照剂量HD50作为主要的辐射敏感性分型指标,分别统计不同函数关系的微核心种质落入不同剂量区间的基因型个数,并依此将259份微核心种质分为敏感型(10)、较敏感型(96)、较钝感型(101)、钝感型(52)。对数函数关系中以敏感型和较敏感型为主,随着γ射线辐照剂量的增加TaKu70和TaKu80基因的相对表达量与对照相比总体升高,但变化不明显;线性函数关系中以较敏感型和较钝感型为主,TaKu70和TaKu80基因的相对表达量与对照相比总体升高,随剂量的增加而逐渐递增;幂函数关系中以较钝感型和钝感型为主,TaKu70和TaKu80基因的相对表达量与对照相比总体升高,但随剂量的增加呈现先增加后降低的趋势,一般相对表达量的峰值出现在150 Gy。     

湾鳄人工饲养的几个常用技术操作

以成都动物园人工饲养的7条湾鳄Crocodylus porosus Schneider为对象,介绍了在人工饲养条件下经常会用到的抓捕、采血、性别鉴定等技术操作及其意义,为湾鳄的人工饲养管理提供一定的实践依据.     

Redescription of a rare deep-sea batfish, <Emphasis Type="Italic">Halieutopsis bathyoreos</Emphasis> (Lophiiformes: Ogcocephalidae)

Halieutopsis bathyoreos Bradbury, 1988 (Lophiiformes: Ogcocephalidae), previously described only on the basis of the holotype (62.6mm in standard length) from the central North Pacific, is redescribed on the basis of the holotype and six additional specimens (41.2–68.7mm in standard length) collected from the western South Pacific, off Papua New Guinea, and the western North Pacific, including the Japanese Archipelago. Halieutopsis bathyoreos is distinguished from its congeners by having a shelflike rostrum extending anteriorly well beyond the mouth, a dorsal escal lobe slightly bisected ventrally, an illicial cavity square in outline and completely visible in ventral view, and lacking tubercles on the ventral surface of the disk. The following characters are newly added to the diagnoses of this species: rostrum width 21–29% of head length, tubercles on the dorsal surface of the disk about half the diameter of those on the lateral margin, and 13–15 large lateral-line scales on the tail.     

Reverse logistics in effective recovery of products from waste materials

Technical solutions for the recovery of products from waste materials become more and more available. To have these new technologies implemented in a real world, a feasibility study is indispensable. For this purpose, it is often imperative to adopt the viewpoint of an individual firm and ask whether it would be wise to engage in product recovery activities or not. Aspects of economics and logistics are of prime importance here. Some important frameworks, models, and insights that have been developed in recent years are described in this paper.     

High throughput cryopreservation of 140,000 Physcomitrella patens mutants

A high throughput protocol was established to preserve 140,000 mutants of a moss, Physcomitrella patens, a model plant for functional genomics studies, over liquid nitrogen. Regarding the reliable long-term storage of diverse mutant phenotypes, as well as time and cost effectiveness, each working step was optimized: 1) plant preparation, 2) freezing regime, cryogenic conditions, 3) regrowth after thawing. A prerequisite for maximum regrowth was a 1-week preculture of chopped plant material on a supplemented medium prior to freezing. Cryo vials as preculture vessels resulted in identical regrowth rates, compared to petri dishes. The cryo vial type had a significant influence on regrowth rates. A cooling rate of - 1 degrees C/min down to - 35 degrees C with a 10 min holding time before transferring plants to - 152 degrees C was the most suitable freezing regime. This protocol allows a cryopreservation of 1100 plants during a 5-day working week, practicable by one person. For more than 650 cryopreserved mutants a maximum regrowth rate of 100 % was obtained, independently of mutant phenotypes.