全文获取类型
收费全文 | 468篇 |
免费 | 66篇 |
国内免费 | 12篇 |
出版年
2024年 | 4篇 |
2023年 | 20篇 |
2022年 | 25篇 |
2021年 | 23篇 |
2020年 | 15篇 |
2019年 | 13篇 |
2018年 | 11篇 |
2017年 | 5篇 |
2016年 | 13篇 |
2015年 | 17篇 |
2014年 | 24篇 |
2013年 | 41篇 |
2012年 | 29篇 |
2011年 | 28篇 |
2010年 | 17篇 |
2009年 | 25篇 |
2008年 | 28篇 |
2007年 | 22篇 |
2006年 | 31篇 |
2005年 | 21篇 |
2004年 | 23篇 |
2003年 | 14篇 |
2002年 | 13篇 |
2001年 | 11篇 |
2000年 | 10篇 |
1999年 | 5篇 |
1998年 | 13篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有546条查询结果,搜索用时 234 毫秒
41.
Katayama K Wada K Miyoshi H Ohashi K Tachibana M Furuki R Mizuguchi H Hayakawa T Nakajima A Kadowaki T Tsutsumi Y Nakagawa S Kamisaki Y Mayumi T 《FEBS letters》2004,560(1-3):178-182
Peroxisome proliferator-activated receptor γ (PPARγ) plays a central role in adipocyte differentiation and insulin sensitivity. Although PPARγ also appears to regulate diverse cellular processes in other cell types such as lymphocytes, the detailed mechanisms remain unclear. In this study, we established a lentivirus-mediated short hairpin RNA expression system and identified a potent short hairpin RNA which suppresses PPARγ expression, resulting in marked inhibition of preadipocyte-to-adipocyte differentiation in 3T3-L1 cells. Our PPARγ-knockdown method will serve to clarify the PPARγ pathway in various cell types in vivo and in vitro, and will facilitate the development of therapeutic applications for a variety of diseases. 相似文献
42.
de Meeûs T Humair PF Grunau C Delaye C Renaud F 《International journal for parasitology》2004,34(8):943-950
Microsatellite loci are usually considered to be neutral co-dominant and Mendelian markers. We undertook to study the inheritance of five microsatellite loci in the European Lyme disease vector, the tick Ixodes ricinus. Only two loci appeared fully Mendelian while the three others displayed non-Mendelian patterns that highly frequent null alleles could not fully explain. At one locus, IR27, some phenomenon seems to hinder the PCR amplification of one allele, depending on its origin (maternal imprinting) and/or its size (short allele dominance). DNA methylation, which appeared to be a possible explanation of this amplification bias, was rejected by a specific test comparing the amplification efficiency that did not differ between unmethylated and experimentally methylated DNA. The role of allele size in heterozygous individuals was then revealed from the data available on field collected ticks and consistent with the results of a theoretical approach. These observations highlight the need for prudence while inferring reproductive systems (selfing rates), parentage or even allelic frequencies from microsatellite markers, in particular for parasitic organisms for which molecular approaches often represent the only way for population biology inferences. 相似文献
43.
Lu WW Hsu YY Yang JY Kung SH 《Biochemical and biophysical research communications》2004,325(2):494-499
44.
Corica F Corsonello A Apolone G Lucchetti M Melchionda N Marchesini G;QUOVADIS Study Group 《Obesity (Silver Spring, Md.)》2006,14(8):1429-1437
Objective: To investigate the construct validity of the Short Form‐36 (SF‐36) Health Survey questionnaire in obese patients. Research Methods and Procedures: Our series consisted of 1735 obese patients (age, 44.7 ± 11.0 years; 1346 women) consecutively enrolled in the QUOVADIS study, an observational multicenter study of obese treatment‐seeking outpatients. The construct validity of the SF‐36 was assessed by main component analysis. Age‐, gender‐, and education‐adjusted general linear models were used to investigate the relationship between BMI and SF‐36 domains or factors identified by main component analysis. Results: BMI was significantly associated with poor health‐related quality of life in all eight SF‐36 domains, and the strongest association was observed with physical activity. Main components analysis generated a six‐factor solution explaining 59% of the observed variance. BMI was strongly associated with factors based on the loading of items regarding the physical activity domain and factors based on role‐physical and role‐emotional items or general health and bodily pain items. In contrast, mental health‐, vitality‐, and social functioning‐based factors were not related to BMI. Discussion: In obese treatment‐seeking outpatients, the clustering of SF‐36 items in main components is not significantly different from the domain‐based approach generally used, thus confirming the robustness of such a generic questionnaire in this specific condition. However, the peculiar clustering of some SF‐36 items and their relationship with BMI suggest that the health‐related quality of life profile of subjects belonging to that population may be better described with alternative aggregations of the SF‐36 items or with disease‐tailored questionnaires. 相似文献
45.
46.
47.
Li SL Yamamoto T Yoshimoto T Uchihi R Mizutani M Kurimoto Y Tokunaga K Jin F Katsumata Y Saitou N 《Human genetics》2006,118(6):695-707
We have analyzed 105 autosomal polymorphic short tandem repeat (STR) loci for nine East and South-eastern Asian populations
(two Japanese, five Han Chinese, Thai, and Burmese populations) and a Caucasian population using a multiplex PCR typing system.
All the STR loci are genomewide tetranucleotide repeat markers of which the total number of observed alleles and the observed
heterozygosity were 756 and 0.743, respectively, for Japanese populations. Phylogenetic analysis for these allele frequency
data suggested that the Japanese populations are more closely related with southern Chinese populations than central and/or
northern ones. STRUCTURE program analysis revealed the almost clearly divided and accountable population structure at K=2–6, that the two Japanese populations always formed one group separated from the other populations and never belong to different
groups at K≥3. Furthermore, our new allele frequency data for 91 loci were analyzed with those for 52 worldwide populations published
by previous studies. Phylogenetic and multidimensional scaling (MDS) analyses indicated that Asian populations with large
population size (six Han Chinese, three Japanese, two Southeast Asia) formed one distinct cluster and are closer to each other
than other ethnic minorities in east and Southeast Asia. This pattern may be the caviar of comparing populations with greatly
differing population sizes when STR loci were analyzed. 相似文献
48.
E. V. Bagatskaya 《Neurophysiology》2006,38(5-6):424-426
Under conditions of the formalin test, we studied changes in the level of analgesia induced by the action of low-intensity
microwaves on the antinociceptive acupuncture point (AP) E36 in mice of strains CBA/CaLac (CBA) and C57BL/6j (C57) and in
albino mongrel mice. Measurements were performed under control conditions and with experimentally induced decrease in the
serotonin level in the brain (by injections of DL-parachlorophenylalanine, p-CPA). In the latter cases, the duration of the pain behavioral reaction increased despite irradiation of the AP E36. In mongrel,
CBA, and C57 mice, the intensity of pain manifestations was 114.4, 29.0, and 21.1% greater, respectively, than in mice of
these groups with no injections of p-CPA. These facts show that the serotonergic brain system is profoundly involved in the formation of analgesia after irradiation
of the AP by low-intensity microwaves, and this involvement significantly depends on the genotype of the animals.
Neirofiziologiya/Neurophysiology, Vol. 38, Nos. 5/6, pp. 495–497, September–December, 2006. 相似文献
49.
Use of DNA markers to study bird migration 总被引:2,自引:2,他引:0
Michael Wink 《Journal of Ornithology》2006,147(2):234-244
The molecular methods that are presently being used for studying phylogenetics, phylogeography and population genetics can
also be applied to study bird migration. They are powerful and can supplement the information obtained from ringing, telemetry,
morphometrics, ringing, radar tracking and isotope analysis. This short review describes the principles, scopes and limitations
DNA methods and DNA markers that are relevant for migration research, such as DNA sequences, short tandem repeats (microsatellites),
single nucleotide polymorphisms, amplified fragment length polymorphism, inter simple sequence repeats and molecular sexing. 相似文献
50.