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81.
Shawn E. Nordell 《Environmental Biology of Fishes》1994,39(3):219-229
Synopsis The mating behavior and dentition ofUrolophus halleri, the round stingray was examined. Males frequently bite females during the mating period but most male biting does not result in copulation. In bites that do not lead to copulation, males bite the posterior (or occasionally the medial) portion of the females' disc but females often free themselves from these bites. In bites that precede copulation, males bite the anterior portion of the females' disc and females do not struggle to free themselves. Thus, females may exert some form of choice when they are bitten. Mature males have sexually dimorphic dentition that may aid in holding females. A principal component analysis (PCA) showed that in juvenile males, the relative size of the teeth decrease while the relative thickness of the disc increases as body size enlarges; adult males displayed no clear pattern. In adult females, there is a relative decrease in tooth size and increase in relative disc thickness as body size enlarges. The relative increase in females disc thickness in areas where they are bitten may function to minimize the amount of damage due to non-copulatory biting. There is no indication that biting functions to induce female receptivity but it may allow females and males to acquire information about potential mates. Thus, copulatory biting functions to maintain contact during copulation while the function of non-copulatory biting is less clear. 相似文献
82.
Effects of light and temperature on gibberellin (GA)-induced seed germination were studied in Arabidopsis thaliana (L.) Heynh. with the use of GA-deficient ( gal ) mutants, mutants with a strongly reduced sensitivity to GA ( gai ) and with the recombinant gai/gal . Seeds of the gal mutant did not germinate in the absence of exogenous GAs, neither in darkness, nor in light, indicating that GAs are absolutely required for germination of this species. Wild-type and gai seeds did not always require applied GAs in light. The conclusion that light stimulates GA biosynthesis was strengthened by the antagonistic action of tetcyclacis, an inhibitor of GA biosynthesis. In wild-type, gal and gai/gal seeds light lowered the GA requirement, which can be interpreted as an increase in sensitivity to GAs. In gai and gai/gal seeds light became effective only after dormancy was broken by either a chilling treatment of one week or a dry after-ripening period at 2°C during some months. The present genetic and physiological evidence strongly suggests that temperature regulates the responsiveness to light in A. thaliana seeds. The responsiveness increases during dormancy breaking, whereas the opposite occurs during induction of dormancy (8 days at 15°C pre-incubation). Since light stimulates the synthesis of GAs as well as the responsiveness to GAs, temperature-induced changes in dormancy may indirectly change the capacities to synthesize GAs and to respond to GAs. GA sensitivity is also directly controlled by temperature. It is concluded that both GA biosynthesis and sensitivity to GAs are not the primary controlling factors in dormancy, but are essential for germination. 相似文献
83.
E. A. Santos R. Keller 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1993,163(5):374-379
The effects of glutamate, aspartate, glycine, proline, alanine, taurine, glycerol, glucose and lactate injections on the haemolymph levels of the crustancean hyperglycemic hormone and/or glucose and lactate in the shore crab, Carcinus maenas, were investigated. Only glucose and lactate caused significant changes of hyperglycaemic hormone levels. Glucose injections resulted in a drop of both hormone and lactate, while lactate had an opposite effect, i.e. it raised both crustacean hormone and glucose levels. The results suggest that during increases in glycolytic flux, lactate may cause a release of hormone by a positive feedback mechanism. The hormone would then stimulate glycogenolysis, thus increasing glucose availability. If more glucose is released than is metabolized, excess glucose may leak from the cells and suppress crustancean hyperglycemic hormone release from the X-organ/sinus gland complex by negative feedback.Abbreviations ABTS
2,2-azino-bis (3-ethylbenzthiazoline sulphonic acid)
- ANOVA
one-way analysis of variance
- BSA
bovine serum albumin
- BW
body weight
- CHH
crustacean hyperglycemic hormone
- ELISA
cnzyme-liked immunosorbent assay
- GIH
gonadinhibiting hormone
- IgG
immunoglobin G
- MIH
moult-inhibiting hormone
- MTGXO
medulla terminalis X-organ
- PB
sodium phosphate buffer
- PBS
phosphate buffered saline
- Pi
inorganic phosphate
- XO-SG
X-organ-sinus gland complex 相似文献
84.
E. J. Eisen M. Fortman W. Y. Chen J. J. Kopchick 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,87(1-2):161-169
The effects of a high-growth genetic background on the growth of mice hemizygous for one of two growth hormone transgenes were examined. Male mice hemizygous for wild-type (W) and dwarf mutant (M) bovine growth hormone (bGH) transgenes were crossed with females of a high-growth selected (S) and control (C) line as follows: W x S, W x C, M x S and M x C. Body weights of progeny were recorded weekly from 2 to 10 weeks of age. F1 progeny were classified as carriers (P) or non-carriers (N) of the transgene by assaying tail DNA for bGH using the polymerase chain reaction and agarose gel electrophoresis. A deficiency in the number of f1 progeny carrying the W (P<0.05) and M (P<0.01) bGH transgene was most likely due to differential prenatal and early postnatal mortality. Bodyweight means of wild-type transgenic mice were larger (P < 0.05) than those of non-transgenic littermates by 3 weeks of age in a C background in contrast to 5 weeks in S. The wild-type bGH transgene increased adult body weights more in the C (155%) than in the S (136%) background, indicating transgene expression by selection background interaction (P < 0.05). However, the growth response to the wild-type transgene in the S background was still large. The dwarf mutant transgene had a greater effect on growth reduction in the S (70%) than in the C (84%) background, thus causing transgene expression by selection background interaction (P < 0.05). Gender by wild-type transgene effect interactions (P < 0.001) for adult body weight were caused by the transgene reducing the gender difference for body weight in C and eliminating it in S. The dwarf mutant caused a larger negative effect on growth in males than in females, resulting in a gender by dwarf mutant transgene interaction (P < 0.001) for adult body weights. Results indicate that the effect of a GH transgene on growth can be affected both by a high-growth genetic background and the gender of progeny. 相似文献
85.
Jacek Najda Jan Gmiński Marian Dróżdż Franciszek Zych 《Biological trace element research》1993,37(2-3):101-106
The influence of silicon-treatment on the levels of TSH and thyroid hormones was studied in rats. Concentrations of thyrotropin
(TSH), triiodothyronine (T3), and thyroxine (T4) were estimated in sera of rats receiving per os a soluble silicon compound—sodium metasilicate nonahydrate (Na2SiO3·9H2O), dissolved in the animals' drinking water. An increase in the TSH level in the tested group was observed, without statistically
significant differences in T3 and T4 concentrations between the two groups of animals. The results provide evidence for the influence of silicon on the endocrine
balance. They could also prove that this chemical element is capable of modifying the rate of some hormones' synthesis. 相似文献
86.
David Crews Judith M. Bergeron James J. Bull Deborah Flores Alan Tousignant James K. Skipper Thane Wibbels 《Genesis (New York, N.Y. : 2000)》1994,15(3):297-312
In many egg-laying reptiles, the incubation temperature of the egg determines the sex of the offspring, a process known as temperature-dependent sex determination (TSD). In TSD sex determination is an “all or none” process and intersexes are rarely formed. How is the external signal of temperature transduced into a genetic signal that determines gonadal sex and channels sexual development? Studies with the red-eared slider turtle have focused on the physiological, biochemical, and molecular cascades initiated by the temperature signal. Both male and female development are active processes—rather than the crganized/default system characteristic of vertebrates with genotypic sex determination—that require simultaneous activation and suppression of testis- and ovary-determining cascades for normal sex determination. It appears that temperature accomplishes this end by acting on genes encoaing for steroidogenic enzymes and steroid hormone receptors and modifying the endocrine microenvironment in the embryo. The temperature experienced in development also has long-term functional outcomes in addition to sex determination. Research with the leopard gecko indicates that incubation temperature as well as steroid hormones serve as organizers in shaping the adult phenotype, with temperature modulating sex hormone action in sexual differentiation. Finally, practical applications of this research have emerged for the conservation and restoration of endangered egg-laying reptiles as well as the embryonic development of reptiles as biomarkers to monitor the estrogenic effects of common environmental contaminants. © 1994 Wiley-Liss, Inc. 相似文献
87.
William Pr Hayes 《Developmental neurobiology》1994,25(9):1095-1112
How neurons diversify in developing brain to produce discrete cell fates in their appropriate regions remains a fundamental question. Embryonic Xenopus was previously used to identify juxtaposed embryonic cells that first express proopiomelanocortin mRNA in forebrain and pituitary, supporting the idea that this neuropeptide phenotype is induced locally. (Hayes and Loh, 1990, Development 110:747–757). To begin to examine how a more widespread population of forebrain cells is set up, the present focus is on the thyrotropin-releasing hormone (TRH) phenotype. Serial section in situ hybridization histochemistry produced the unexpected finding that the adult-like TRH system spanning forebrain and comprising over six different telencephalic and diencephalic nuclei, is preceded by an embryonic TRH cell population that is initially localized and then highly regionalized in the area from which the adult pattern develops. Thus, the first TRH cells, detected in vivo after 35 h (stage 29/30), were confined to discrete anterior or posterior bilateral clusters in embryonic forebrain or hindbrain. Thereafter, the TRH cell clusters in diencephelon, but not hindbrain, expanded to form rows, extending anteriorly into telencephalon and bifurcating posteriorly around the infundibulum. By 80 h (stage 42), after extensive brain morphogenesis, these forebrain rows showed regional differences in levels of TRH and mRNA corresponding to the specific brain nuclei that have been shown to contain TRH cells in adult. These findings show that subsets of phenotype-specific forebrain cell first form a regionalized neuronal cell fate before distinct brain nuclei form. This is turn points to the testable hypothesis in Xenopus that certain neuronal cell fates in forebrain may be dictated by cell lineage or local induction. 1994 John Wiley & Sons, Inc. 相似文献
88.
A variety of fluorescein isothiocyanate-labeled lectins specific for different sugar moieties were examined as probes for the wound-healing response in the filamentous red alga Antithamnion sparsum Tokida. Among them, only concanavalin A (ConA) and Lens culrinaris agglutinin (LCA), which have specificity to α-D-mannosyl residues, bound specifically to repair cells during the wound-healing process. When ConA or LCA was added at various time intervals after wounding, it first bound (3 h post-wounding) as a thin layer at the tips of the adjacent cells. Later (4–5 h post-wounding) labeling also appeared at the tips of the repair cells. Intense labeling at these sites continued throughout the healing process until repair cell fusion, at which time the lectin labeling was reduced to a narrow ring around the area of fusion. When added to plants prior to wounding and continually monitored, these same lectins acted as inhibitors to the wound-healing response. Other control lectins showed no inhibitory effects. A crude extract solution obtained from decapitated filaments stimulated the wound-healing response, and a lectin-binding component bound strongly to a protein-binding transfer membrane. These results suggest that the labeled compound is a glycoprotein that has α-D-mannosyl residues and is similar to the repair hormone rhodomorphin found in Griffithsia pacifica Kylin. 相似文献
89.
Simon Y. C. Wong Geoffrey R. Guile Thomas W. Rademacher Raymond A. Dwek 《Glycoconjugate journal》1993,10(3):227-234
Glycopeptides can be valuable tools in determining the influence of carbohydrate moieties on the intrinsic properties of glycoproteins. However, glycopeptides of sufficient quantity and purity are as yet not readily available from biological sources. The chemical coupling of a -glycosylamino group of an unprotected carbohydrate with an activated aspartic acid residue of an unprotected peptide is a simple method for synthesizing asparagine-linked glycopeptides. In this report we demonstrate that the use of this method is not restricted to -glycosylamines of simple monosaccharides or short aspartic acid-containing pentapeptides. This is illustrated by the syntheses of several glycopentapeptides containingN,N-diacetylchitobiose, a glutamine-linked glycopentapeptide containing a biantennary complex oligosaccharide, and glycosylated variants of two analogs of a polypeptide hormone, atriopeptin, containingN,N-diacetylchitobiose.Abbreviations Ac
acetyl
- Bzl
benzyl
- DMF
dimethylformamide
- Fmoc
9-fluorenylmethoxycarbonyl
- Fuc
fucose
- Gal
galactose
- GlcNAc
N-acetylglucosamine
- HBTU
O-benzotriazol-1-yl-N,N,N,N-tetramethyluroniumhexa-fluorophosphate
- HOBt
1-hydroxybenzotriazole
- Man
mannose
-
m/z
mass/charge
- NMR
nuclear magnetic resonance
- Xyl
xylose
- Z
benzyloxycarbonyl; unless otherwise specified, amino acids are abbreviated using their one-letter codes. 相似文献
90.
L. O. Greenfield 《Human Evolution》1993,8(3):187-204
A tooth at the border between two morphogenetic fields (mandibular canine and honing premolar) may become morphologically
similar to and/or functionally incorporated with the teeth of either field. In light of this, observations of the morphology
and occlusion of female anthropoid C1s from 58 extant species are presented to assess whether and to what extent they exhibit incisor-like form and function. Female
C1s in 74% of the taxa observed exhibit well developed incisor-like traits which may reflect field border phenomena. In another
9%, incisor traits are present but they are not examples of field border phenomena. Interspecies variation in female C1 morphology is related to behavior, function, natural selection and phyletic inertia. A selection model, derived from the
data, is used to explain C1 sexual dimorphism and the evolution of male and female human canines. The data's relevance to the field vs clone theory debate
is also discussed. 相似文献