全文获取类型
收费全文 | 2483篇 |
免费 | 136篇 |
国内免费 | 227篇 |
出版年
2023年 | 19篇 |
2022年 | 27篇 |
2021年 | 39篇 |
2020年 | 49篇 |
2019年 | 43篇 |
2018年 | 49篇 |
2017年 | 50篇 |
2016年 | 48篇 |
2015年 | 60篇 |
2014年 | 115篇 |
2013年 | 155篇 |
2012年 | 114篇 |
2011年 | 114篇 |
2010年 | 92篇 |
2009年 | 126篇 |
2008年 | 132篇 |
2007年 | 131篇 |
2006年 | 133篇 |
2005年 | 101篇 |
2004年 | 107篇 |
2003年 | 104篇 |
2002年 | 95篇 |
2001年 | 70篇 |
2000年 | 68篇 |
1999年 | 73篇 |
1998年 | 67篇 |
1997年 | 53篇 |
1996年 | 70篇 |
1995年 | 55篇 |
1994年 | 52篇 |
1993年 | 41篇 |
1992年 | 49篇 |
1991年 | 48篇 |
1990年 | 30篇 |
1989年 | 36篇 |
1988年 | 31篇 |
1987年 | 18篇 |
1986年 | 23篇 |
1985年 | 31篇 |
1984年 | 28篇 |
1983年 | 17篇 |
1982年 | 22篇 |
1981年 | 11篇 |
1980年 | 7篇 |
1979年 | 8篇 |
1978年 | 7篇 |
1976年 | 10篇 |
1975年 | 4篇 |
1973年 | 3篇 |
1972年 | 3篇 |
排序方式: 共有2846条查询结果,搜索用时 15 毫秒
901.
Background
Nucleotide metabolism is central to all biological systems, due to their essential role in genetic information and energy transfer, which in turn suggests its possible presence in the last common ancestor (LCA) of Bacteria, Archaea and Eukarya. In this context, elucidation of the contribution of the origin and diversification of de novo and salvage pathways of nucleotide metabolism will allow us to understand the links between the enzymatic steps associated with the LCA and the emergence of the first metabolic pathways.Results
In this work, the taxonomical distribution of the enzymes associated with nucleotide metabolism was evaluated in 1,606 complete genomes. 151 sequence profiles associated with 120 enzymatic reactions were used. The evaluation was based on profile comparisons, using RPS-Blast. Organisms were clustered based on their taxonomical classifications, in order to obtain a normalized measure of the taxonomical distribution of enzymes according to the average of presence/absence of enzymes per genus, which in turn was used for the second step, to calculate the average presence/absence of enzymes per Clade.Conclusion
From these analyses, it was suggested that divergence at the enzymatic level correlates with environmental changes and related modifications of the cell wall and membranes that took place during cell evolution. Specifically, the divergence of the 5-(carboxyamino) imidazole ribonucleotide mutase to phosphoribosylaminoimidazole carboxylase could be related to the emergence of multicellularity in eukaryotic cells. In addition, segments of salvage and de novo pathways were probably complementary in the LCA to the synthesis of purines and pyrimidines. We also suggest that a large portion of the pathway to inosine 5’-monophosphate (IMP) in purines could have been involved in thiamine synthesis or its derivatives in early stages of cellular evolution, correlating with the fact that these molecules may have played an active role in the protein-RNA world. The analysis presented here provides general observations concerning the adaptation of the enzymatic steps in the early stages of the emergence of life and the LCA.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-800) contains supplementary material, which is available to authorized users. 相似文献902.
Background
Several resistance traits, including the I2 resistance against tomato fusarium wilt, were mapped to the long arm of chromosome 11 of Solanum. However, the structure and evolution of this locus remain poorly understood.Results
Comparative analysis showed that the structure and evolutionary patterns of the I2 locus vary considerably between potato and tomato. The I2 homologues from different Solanaceae species usually do not have orthologous relationship, due to duplication, deletion and frequent sequence exchanges. At least 154 sequence exchanges were detected among 76 tomato I2 homologues, but sequence exchanges between I2 homologues in potato is less frequent. Previous study showed that I2 homologues in potato were targeted by miR482. However, our data showed that I2 homologues in tomato were targeted by miR6024 rather than miR482. Furthermore, miR6024 triggers phasiRNAs from I2 homologues in tomato. Sequence analysis showed that miR6024 was originated after the divergence of Solanaceae. We hypothesized that miR6024 and miR482 might have facilitated the expansion of the I2 family in Solanaceae species, since they can minimize their potential toxic effects by down-regulating their expression.Conclusions
The I2 locus represents a most divergent resistance gene cluster in Solanum. Its high divergence was partly due to frequent sequence exchanges between homologues. We propose that the successful expansion of I2 homologues in Solanum was at least partially attributed to miRNA mediated regulation.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-743) contains supplementary material, which is available to authorized users. 相似文献903.
904.
905.
Rahul Metri Gaurav Jerath Govind Kailas Nitin Gacche Adityabarna Pal Vibin Ramakrishnan 《Protein science : a publication of the Protein Society》2014,23(1):117-120
A reduced representation in the format of a barcode has been developed to provide an overview of the topological nature of a given protein structure from 3D coordinate file. The molecular structure of a protein coordinate file from Protein Data Bank is first expressed in terms of an alpha‐numero code and further converted to a barcode image. The barcode representation can be used to compare and contrast different proteins based on their structure. The utility of this method has been exemplified by comparing structural barcodes of proteins that belong to same fold family, and across different folds. In addition to this, we have attempted to provide an illustration to (i) the structural changes often seen in a given protein molecule upon interaction with ligands and (ii) Modifications in overall topology of a given protein during evolution. The program is fully downloadable from the website http://www.iitg.ac.in/probar/ . 相似文献
906.
Computational prediction of the origin of replication is a challenging problem and of immense interest to biologists. Several methods have been proposed for identifying the replicon site for various classes of organisms. However, these methods have limited applicability since the replication mechanism is different in different organisms. We propose a correlation measure and show that it is correctly able to predict the origin of replication in most of the bacterial genomes. When applied to Methanocaldococcus jannaschii, Plasmodium falciparum apicoplast and Nicotiana tabacum plastid, this correlation based method is able to correctly predict the origin of replication whereas the generally used GC skew measure fails. Thus, this correlation based measure is a novel and promising tool for predicting the origin of replication in a wide class of organisms. This could have important implications in not only gaining a deeper understanding of the replication machinery in higher organisms, but also for drug discovery. 相似文献
907.
908.
To screen and compare the differentially expressed genes between one MDR-TB strain separated from one child patient and the virulent Mycobacterium tuberculosis H37Rv, suppression subtractive hybridization (SSH) technology was used to build a library of cDNAs that were differentially expressed in the MDR and H37Rv. From this cDNA library, genes that were expressed in the MDR-TB but not in the H37Rv were selected for gene sequencing and homology analysis; 113 positive clones were obtained, their cDNA fragments were sequenced, and homology analysis was performed. Four novel sequences were identified. The results provide a partial list of genes differentially expressed in MDR-TB and four novel genes were found. Identification of these genes may contribute to our understanding of MDR-TB development. 相似文献
909.
910.