全文获取类型
收费全文 | 3778篇 |
免费 | 395篇 |
国内免费 | 592篇 |
出版年
2024年 | 15篇 |
2023年 | 82篇 |
2022年 | 87篇 |
2021年 | 140篇 |
2020年 | 154篇 |
2019年 | 171篇 |
2018年 | 157篇 |
2017年 | 161篇 |
2016年 | 160篇 |
2015年 | 146篇 |
2014年 | 196篇 |
2013年 | 254篇 |
2012年 | 157篇 |
2011年 | 156篇 |
2010年 | 144篇 |
2009年 | 198篇 |
2008年 | 181篇 |
2007年 | 202篇 |
2006年 | 190篇 |
2005年 | 172篇 |
2004年 | 117篇 |
2003年 | 132篇 |
2002年 | 105篇 |
2001年 | 88篇 |
2000年 | 81篇 |
1999年 | 103篇 |
1998年 | 74篇 |
1997年 | 85篇 |
1996年 | 57篇 |
1995年 | 69篇 |
1994年 | 71篇 |
1993年 | 74篇 |
1992年 | 70篇 |
1991年 | 65篇 |
1990年 | 44篇 |
1989年 | 44篇 |
1988年 | 45篇 |
1987年 | 41篇 |
1986年 | 29篇 |
1985年 | 40篇 |
1984年 | 32篇 |
1983年 | 17篇 |
1982年 | 29篇 |
1981年 | 26篇 |
1980年 | 17篇 |
1979年 | 19篇 |
1978年 | 28篇 |
1977年 | 12篇 |
1976年 | 7篇 |
1974年 | 6篇 |
排序方式: 共有4765条查询结果,搜索用时 15 毫秒
991.
Associations of polymorphisms in TXNIP and gene–environment interactions with the risk of coronary artery disease in a Chinese Han population 下载免费PDF全文
Shuai Zhang Ning‐hua Cui Ze‐jin Liu Zhu‐liang Huang Cong Li Fang Zheng 《Journal of cellular and molecular medicine》2016,20(12):2362-2373
Single nucleotide polymorphisms (SNPs) in thioredoxin‐interacting protein (TXNIP) gene may modulate TXNIP expression, then increase the risk of coronary artery disease (CAD). In a two‐stage case–control study with a total of 1818 CAD patients and 1963 controls, we genotyped three SNPs in TXNIP and found that the variant genotypes of SNPs rs7212 [odds ratio (OR) = 1.26, P = 0.001] and rs7211 (OR = 1.23, P = 0.005) were significantly associated with increased CAD risk under a dominant model. In haplotype analyses, compared with the reference haplotype, haplotype ‘G‐T’ had a 1.22‐fold increased risk of CAD (P = 0.003). We also observed the cumulative effects of SNPs rs7212 and rs7211 on CAD risk and the severity of coronary atherosclerosis. Moreover, the gene–environment interactions among the variant genotypes of SNP rs7212, smoking habit, alcohol drinking habit and history of type 2 diabetes were associated with a 3.70‐fold increased risk of CAD (P < 0.001). Subsequent genotype‐phenotype correlation analyses further observed the significant effects of SNP rs7212 on TXNIP mRNA expression, plasma TXNIP and malondialdehyde levels. Taken together, our data suggest that TXNIP SNPs may individually and cumulatively affect CAD risk through a possible mechanism for regulating TXNIP expression and gene–environment interactions. 相似文献
992.
993.
There is a substantial body of research reporting evidence of associations between various forms of childhood adversity and psychosis, across the spectrum from experiences to disorder. This has been extended, more recently, to include studies of cumulative effects, of interactions with other factors, of specific effects, and of putative biological and psychological mechanisms. In this paper we evaluate this research and highlight the remaining methodological issues and gaps that temper, but do not dismiss, conclusions about the causal role of childhood adversity. We also consider the emerging work on cumulative, synergistic, and specific effects and on mechanisms; and discuss the broader implications of this line of research for our understanding of psychosis. We conclude that the current balance of evidence is that childhood adversities – particularly exposure to multiple adversities involving hostility and threat – do, in some people, contribute to the onset of psychotic experiences and psychotic disorders. 相似文献
994.
Dynamic changes in host–virus interactions associated with colony founding and social environment in fire ant queens (Solenopsis invicta) 下载免费PDF全文
The dynamics of host – parasite interactions can change dramatically over the course of a chronic infection as the internal (physiological) and external (environmental) conditions of the host change. When queens of social insects found a colony, they experience changes in both their physiological state (they develop their ovaries and begin laying eggs) and the social environment (they suddenly stop interacting with the other members of the mother colony), making this an excellent model system for examining how these factors interact with chronic infections. We investigated the dynamics of host – viral interactions in queens of Solenopsis invicta (fire ant) as they transition from mating to colony founding/brood rearing to the emergence of the first workers. We examined these dynamics in naturally infected queens in two different social environments, where queens either founded colonies as individuals or as pairs. We hypothesized that stress associated with colony founding plays an important role in the dynamics of host – parasite interactions. We also hypothesized that different viruses have different modalities of interaction with the host that can be quantified by physiological measures and genomic analysis of gene expression in the host. We found that the two most prevalent viruses, SINV‐1 and SINV‐2, are associated with different fitness costs that are mirrored by different patterns of gene expression in the host. In fact SINV‐2, the virus that imposes the significant reduction of a queen's reproductive output is also associated with larger changes of global gene expression in the host. These results show the complexity of interactions between S. invicta and two viral parasites. Our findings also show that chronic infections by viral parasites in insects are dynamic processes that may pose different challenges in the host, laying the groundwork for interesting ecological and evolutionary considerations. 相似文献
995.
The strength of the association between heterozygosity and probability of interannual local recruitment increases with environmental harshness in blue tits 下载免费PDF全文
Esperanza S. Ferrer Vicente García‐Navas Juan José Sanz Joaquín Ortego 《Ecology and evolution》2016,6(24):8857-8869
The extent of inbreeding depression and the magnitude of heterozygosity–fitness correlations (HFC) have been suggested to depend on the environmental context in which they are assayed, but little evidence is available for wild populations. We combine extensive molecular and capture–mark–recapture data from a blue tit (Cyanistes caeruleus) population to (1) analyze the relationship between heterozygosity and probability of interannual adult local recruitment and (2) test whether environmental stress imposed by physiologically suboptimal temperatures and rainfall influence the magnitude of HFC. To address these questions, we used two different arrays of microsatellite markers: 14 loci classified as neutral and 12 loci classified as putatively functional. We found significant relationships between heterozygosity and probability of interannual local recruitment that were most likely explained by variation in genomewide heterozygosity. The strength of the association between heterozygosity and probability of interannual local recruitment was positively associated with annual accumulated precipitation. Annual mean heterozygosity increased over time, which may have resulted from an overall positive selection on heterozygosity over the course of the study period. Finally, neutral and putatively functional loci showed similar trends, but the former had stronger effect sizes and seemed to better reflect genomewide heterozygosity. Overall, our results show that HFC can be context dependent, emphasizing the need to consider the role of environmental heterogeneity as a key factor when exploring the consequences of individual genetic diversity on fitness in natural populations. 相似文献
996.
Effects of larval crowding on quantitative variation for development time and viability in Drosophila melanogaster 下载免费PDF全文
Competition between individuals belonging to the same species is a universal feature of natural populations and is the process underpinning organismal adaptation. Despite its importance, still comparatively little is known about the genetic variation responsible for competitive traits. Here, we measured the phenotypic variation and quantitative genetics parameters for two fitness‐related traits—egg‐to‐adult viability and development time—across a panel of Drosophila strains under varying larval densities. Both traits exhibited substantial genetic variation at all larval densities, as well as significant genotype‐by‐environment interactions (GEIs). GEI was attributable to changes in the rank order of reaction norms for both traits, and additionally to differences in the between‐line variance for development time. The coefficient of genetic variation increased under stress conditions for development time, while it was higher at both high and low densities for viability. While development time also correlated negatively with fitness at high larval densities—meaning that fast developers have high fitness—there was no correlation with fitness at low density. This result suggests that GEI may be a common feature of fitness‐related genetic variation and, further, that trait values under noncompetitive conditions could be poor indicators of individual fitness. The latter point could have significant implications for animal and plant breeding programs, as well as for conservation genetics. 相似文献
997.
Plants adapted to warmer climate do not outperform regional plants during a natural heat wave 下载免费PDF全文
Anna Bucharova Walter Durka Julia‐Maria Hermann Norbert Hölzel Stefan Michalski Johannes Kollmann Oliver Bossdorf 《Ecology and evolution》2016,6(12):4160-4165
With ongoing climate change, many plant species may not be able to adapt rapidly enough, and some conservation experts are therefore considering to translocate warm‐adapted ecotypes to mitigate effects of climate warming. Although this strategy, called assisted migration, is intuitively plausible, most of the support comes from models, whereas experimental evidence is so far scarce. Here we present data on multiple ecotypes of six grassland species, which we grew in four common gardens in Germany during a natural heat wave, with temperatures 1.4–2.0°C higher than the long‐term means. In each garden we compared the performance of regional ecotypes with plants from a locality with long‐term summer temperatures similar to what the plants experienced during the summer heat wave. We found no difference in performance between regional and warm‐adapted plants in four of the six species. In two species, regional ecotypes even outperformed warm‐adapted plants, despite elevated temperatures, which suggests that translocating warm‐adapted ecotypes may not only lack the desired effect of increased performance but may even have negative consequences. Even if adaptation to climate plays a role, other factors involved in local adaptation, such as biotic interactions, may override it. Based on our results, we cannot advocate assisted migration as a universal tool to enhance the performance of local plant populations and communities during climate change. 相似文献
998.
Tracy A. Nichols Terry R. Spraker Thomas Gidlewski Bruce Cummings Dana Hill Qingzhong Kong 《朊病毒》2016,10(3):228-250
Chronic wasting disease (CWD), the only known wildlife prion disease, affects deer, elk and moose. The disease is an ongoing and expanding problem in both wild and captive North American cervid populations and is difficult to control in part due to the extreme environmental persistence of prions, which can transmit disease years after initial contamination. The role of exogenous factors in CWD transmission and progression is largely unexplored. In an effort to understand the influence of environmental and dietary constituents on CWD, we collected and analyzed water and soil samples from CWD-negative and positive captive cervid facilities, as well as from wild CWD-endozootic areas. Our analysis revealed that, when compared with CWD-positive sites, CWD-negative sites had a significantly higher concentration of magnesium, and a higher magnesium/copper (Mg/Cu) ratio in the water than that from CWD-positive sites. When cevidized transgenic mice were fed a custom diet devoid of Mg and Cu and drinking water with varied Mg/Cu ratios, we found that higher Mg/Cu ratio resulted in significantly longer survival times after intracerebral CWD inoculation. We also detected reduced levels of inflammatory cytokine gene expression in mice fed a modified diet with a higher Mg/Cu ratio compared to those on a standard rodent diet. These findings indicate a role for dietary Mg and Cu in CWD pathogenesis through modulating inflammation in the brain. 相似文献
999.
Ralph B. L. Gwatkin Martin M. Quigley Robert L. Collins 《Molecular reproduction and development》1989,23(2):229-232
A study of mouse gamete processing for in vitro fertilization (IVF) under various conditions showed that it is necessary to control the atmosphere if the temperature is raised from 22°C to 37°C. The data suggest that maximum IVF success is attained by processing the gametes at 37°C, under an atmosphere of 5% O2 and 5% CO2, and overlaying the medium with silicone oil. 相似文献
1000.
R. V. Molina F. Nuez J. Cuartero M. L. Goméz-Guillamón J. Abadía 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,78(3):411-416
Summary Six fruit characters have been measured in 23 cultivars of Cucumis melo, representing a wide geographical range. Plants were grown both in the greenhouse and in the field. When the 23 cultivars were analyzed together, the largest component of variance was found between cultivars under both growth conditions, suggesting the existence of large genetic diversity for all the characters studied. Generally, variance between plants within cultivars was less than or equal to variance between fruits within plant. This indicates that environmental variation is the most important part of the variation within cultivars. Correlations between pairs of characters at cultivar, plant and fruit levels were calculated from the variance-covariance components. In the majority of paired traits, the correlation values indicated that genetic and environmental factors may act in the same direction. 相似文献