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81.
Immunocytochemical localization of S-100 protein in astrocytes and Müller cells in the rabbit retina
Jutta Schnitzer 《Cell and tissue research》1987,248(1):55-61
Summary The localization of S-100 protein was studied in histological sections of retinae from adult rabbits. By use of double-immunolabeling techniques it was shown that most but not all radially oriented vimentin-positive Müller cells were co-labeled by an antiserum to S-100 protein. Glial fibrillary acidic protein-positive astrocytes, which in the rabbit retina are restricted to the medullary rays formed by myelinated optic nerve fibers, consistently showed S-100 protein immunoreactivity. The present report shows that, with respect to S-100 protein staining, Müller cells represent a heterogeneous population of glial elements. 相似文献
82.
Osamu Sato Takeyasu Maeda Shigeo Kobayashi Toshihiko Iwanaga Tsuneo Fujita Yasuo Takahashi 《Cell and tissue research》1988,251(1):13-21
Summary Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the nerve fibers and their terminations differed markedly from those in molars. NFP-positive, thick nerve bundles entered the lingual periodontal ligament through slits located in the mid-region of the alveolar socket, and immediately formed numerous Ruffini-like corpuscles. In the labial periodontal ligament, all of the NFP-immunoreactive nerve fibers terminated in free endings. The restricted location of the stretch receptor, Ruffini-like corpuscle, in the lingual periodontal ligament appears to be an essential element, because this region is regularly extended during mastication. The nervous elements were restricted to the alveolar half of the periodontal ligament in every region; they avoided the dental half of the periodontal ligament, which presumably moves continuously with the tooth. Pulpal nerve fibers in incisors also showed a characteristic distribution different from those in molars; individual nerve fibers with beaded structures ran in the center of the pulp toward the incisai edge, and did not form the subodontoblastic nerve plexus of Raschkow.Immunostaining for S-100 protein revealed a distribution pattern of nervous elements similar to that for NFP, suggesting that the nerves supplying the periodontal ligament and dental pulp were mostly covered by a Schwann sheath. 相似文献
83.
本实验利用核磁共振技术,对体内培养了腹水癌小鼠肝细胞基因组DNA的结构变化进行了测试,发现随致癌时间的延长,基因组DNA内胸腺嘧啶核苷酸周围的化学环境发生了定向的变化,造成DNA核磁共振氢谱中,部分胸腺嘧啶的甲基质子发生了化学位移,在′H-NMR图谱中1.9ppm峰Ⅰ减小,而2.6-2.7ppm区域的峰Ⅱ却明显增加,结果使双峰比(峰Ⅰ积分面积/峰Ⅱ积分面积)显著下降,本文为研究癌症发生和发展的遗传学机制提供了一个新的方法. 相似文献
84.
Several procedures were employed to examine the in vitro interaction between S-100 proteins and microtubule proteins. Binding of S-100 to tau factors was observed under all experimental conditions. S-100 binding to microtubule-associated protein 2 (MAP2) was best detected by exposing nitrocellulose-immobilized MAP2 or MAPs to either 125I-labeled S-100 or biotinylated S-100. S-100 binding to tubulin was detected when the two protein fractions were first incubated with each other followed by exposure to the bifunctional cross-linker disuccinimidylsuberate, and then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transfered onto nitrocellulose paper. By this procedure, complex formation between S-100 and tubulin, as well as between S-100 and a relatively low-molecular-weight MAP, was evidenced by immunoblotting using an anti-S-100 antiserum. Alternatively, complex formation between biotinylated S-100 and either tubulin or MAPs was visualized by means of avidin-peroxidase, after SDS-PAGE of the complex mixtures and transfer of the separated proteins onto nitrocellulose. The interaction between S-100 and tubulin was strictly Ca2+ dependent, and resistant to high concentrations of KCl, colchicine, or vinblastine. 相似文献
85.
Dr. Tatsuo Ushiki Toshihiko Iwanaga Toru Masuda Yasuo Takahashi Tsuneo Fujita 《Cell and tissue research》1984,235(3):509-514
Summary The present study deals with the localization and ultrastructure of S-100-immunoreactive cells in the human thymus. These immunoreactive cells are distributed mainly in the medulla with some scattered elements in the cortex. Electron-microscopic observation revealed that the cells are characterized by an irregularly shaped nucleus, tubulovesicular structures in the cytoplasm and characteristic interdigitations of the plasma membrane. The cells often embrace lymphocytes with their branched processes. On the basis of these morphological features, the immunostained elements were identified as interdigitating cells (IDCs). The immunocytochemistry for S-100 visualizes the precise distribution and extension of the IDCs under the light microscope and indicates that the IDCs form no structural networks such as those established by the thymic epithelial cells. Since the IDCs in human lymph nodes have also been reported to contain S-100-like immunoreactivity, S-100 protein can be regarded as a useful marker for identifying the IDCs in the human thymus and other lymphoid organs. 相似文献
86.
Summary Adrenal glands of human fetuses were investigated by means of an immunohistochemical method with the use of an anti-S-100 serum. S-100-immunoreactivity was recognized in sustentacular cells located among the chromaffin cells. A characteristic circular arrangement of the immunostained cells was found in the central region of the adrenal glands. It surrounded aggregations of non-argyrophilic, small, round cells, which were identified as the remaining sympathoblasts (primitive sympathetic cells). 相似文献
87.
88.
A.G.D. El Hag 《欧洲藻类学杂志》2013,48(3):315-319
A coccoid marine cyanobacterium assignable to the genus Synechococcus has been isolated in axenic culture. This organism appears not to be a nitrogen fixer as it failed to reduce acetylene under either aerobic or anaerobic conditions. The specific growth rate was determined at six irradiance levels (5-, 15-, 30-, 50-, 100- and 150 μmol m-2 s-1) under continuous illumination and controlled temperature (20°C). The growth vs. incident irradiance curve and estimated light energy absorbed showed that growth saturates at a relatively low photon flux density and photosynthesis has a low compensation point. It attained its maximum division rate (1·4 divisions d-1 = generation time, 18 h) at 30 μmol m-2 s-1. Such a low light-requiring planktonic cyanobacterium may be of importance in terms of both biomass and productivity towards the bottom of the photic zone. 相似文献
89.
《Bioscience, biotechnology, and biochemistry》2013,77(3):684-690
Twenty-three strains, which were assigned to Gluconobacter frateurii and maintained at Culture Collection NBRC, were re-identified at the species level on the basis of restriction analysis of 16S-23S rDNA ITS regions by digestion with six restriction endonucleases: Bsp1286I, MboII, AvaII, TaqI, BsoBI, and BstNI. The strains examined were divided into six groups, Group III-1, Group III-2, Group III-3, Group III-4, Group III-5, and Group IV. Group III-1 and Group III-4 respectively were divided into two subgroups, Subgroup III-1a, Subgroup III-1b and Subgroup III-4a, Subgroup III-4b. Gluconobacter frateurii NBRC 3264T was included in Group III-2, along with strains NBRC 3265 and NBRC 3270, and G. thailandicus BCC 14116T was included in Group III-3, along with strains NBRC 3254, NBRC 3256, NBRC 3258, NBRC 3255, and NBRC 3257. These groupings were supported by a phylogenetic tree based on 16S-23S rDNA ITS sequences. Strains of group III-2 and Group IV were unequivocally re-identified as G. frateurii, but strains of Group III-3, Group III-4, and Group III-5 were not necessarily re-identified as G. frateurii. The results obtained indicate that the 23 strains have a taxonomically heterogeneous nature, and they are referred to as the G. frateurii complex. 相似文献
90.
Evidence that the actin site is impaired by Ca2+-activated degradation of the heavy chain of dystrophic myosin 总被引:1,自引:0,他引:1
S M Pemrick 《Biochemical and biophysical research communications》1981,102(3):877-894
At pathophysiological concentrations of Ca2+, the heavy chain of dystrophic myosin was degraded by an endogenous protease. This was not the case for normal myosin. However, normal myosin was a substrate of Ca2+-activated neutral protease (CAF) from platelets. This indicated that the endogenous protease in preps of dystrophic myosin was CAF. The pathophysiological effect of heavy chain degradation was restricted to the actin site. Under Vmax conditions hydrolytic activities remained within the normal range, whereas the Kapp of actin for myosin increased 3-fold following extensive heavy chain degradation of dystrophic myosin. Removal of those heavy chain fragments which were soluble at low inoic strength restored Kapp to normal levels. 相似文献