Positron emission tomography ligand activation studies in the sports sciences: measuring neurochemistry in vivo

Functional neuroimaging with magnetic resonance imaging (fMRI) or positron emission tomography (PET) provides the methodology to unravel some of the fascinating, but hitherto largely unresolved interactions between physical exercise and brain function. Phenomena such as raised mood, pain modulation, and sport addiction associated with physical exercise are highly interesting psychophysical models that require further in depth understanding at the neurotransmitter level. PET ligand displacement studies allow in vivo monitoring of endogenous transmitter trafficking in the entire brain and, thereby, to identify the link between exercise-induced behavioral measures and the endogenous neurotransmitter release. This review focuses on the methodology of ligand displacement in the opioidergic system, which together with the dopaminergic system has been considered as a central neurotransmitter system underlying diverse sport-induced psychophysical effects. Understanding the basic principles of exercise-induced transmitter release in the brain will potentially aid clinical applications of endurance training, both as a preventative or therapeutic intervention.     

Effect of Behavioural Feedback on Circadian Clocks of the Nocturnal Field Mouse Mus booduga

The effect of 'novel running wheels' on circadian clocks of the nocturnal field mouse Mus booduga was investigated during free-running and entrained conditions. In order to find out whether daily access to novel running wheels can entrain the locomotor activity rhythms experimental animals (n = 6) were provided with 'novel running wheels' at a fixed time of the day. The control animals (n = 5) were handled similar to the experimental animals but were not given access to novel running wheels. The results show that daily access to novel running wheels entrained the free-running locomotor activity rhythm of these mice. The post-entrainment free-running period (τ) of the experimental animals was significantly shorter than the pre-entrainment τ, whereas the pre- and post-treatment τ of the control animals did not differ significantly. In separate set of experiments, the effect of access to novel running wheels on the rate of re-entrainment was studied after a 6 h phase advance/delay in 24 h (12:12 h) light/dark (LD) cycles. Experimental animals were given access to novel running wheels for 3-h, 1 h after the 'lights-off' only on the first day of the 'new LD cycles'. Experimental animals took fewer cycles to re-entrain to 6-h phase advanced LD cycles compared to the control animals. After a phase delay in the LD cycles by 6h, the experimental animals took more number of cycles to re-entrain compared to the control animals. These results thus suggest that access to novel running wheel can act as a Zeitgeber for the circadian clocks of the nocturnal mouse M. booduga, and can also modify the rates of re-entrainment to phase shifted LD cycles, in a time-dependent manner.     

Adaptive changes in running kinematics as a function of head stability demands and their effect on shock transmission

This study aimed to identify adaptive changes in running kinematics and impact shock transmission as a function of head stability requirements. Fifteen strides from twelve recreational runners were collected during preferred speed treadmill running. Head stability demands were manipulated through real-time visual feedback that required head-gaze orientation to maintain within boxes of different sizes, ranging from 21° to 3° of visual angle with 3° decrements. The main outcome measures were tibial and head peak accelerations in the time and frequency domains (impact and active phases), shock transmission from tibia to head, stride parameters, and sagittal plane joint kinematics. Increasing head stability requirements resulted in decreases in the amplitude and integrated power of head acceleration during the active phase of stance. During the impact portion of stance tibial and head acceleration and shock transmission remained similar across visual conditions. In response to increased head stability requirements, participants increased stride frequency approximately 8% above preferred, as well as hip flexion angle at impact; stance time and knee and ankle joint angles at impact did not change. Changes in lower limb joint configurations (smaller hip extension and ankle plantar-flexion and greater knee flexion) occurred at toe-off and likely contributed to reducing the vertical displacement of the center of mass with increased head stability demands. These adaptive changes in the lower limb enabled runners to increase the time that voluntary control is allowed without embedding additional impact loadings, and therefore active control of the head orientation was facilitated in response to different visual task constraints.     

THREE DAYS OF NOVEL WHEEL ACCESS DIMINISHES LIGHT-INDUCED PHASE DELAYS IN VIVO WITH NO EFFECT ON PER1 INDUCTION BY LIGHT

The mammalian circadian clock, located in the hypothalamic suprachiasmatic nuclei, synchronizes endogenous behavioral and physiological rhythms to a 24h period through responses to two types of stimuli: photic (light) and nonphotic (behaviorally induced arousal and/or increases in activity). Photic stimuli can block nonphotic effects and vice versa, although the mechanisms and levels of interactions between these two stimuli types are unknown. Here, we investigated whether 3 d of access to a novel running wheel alters the phase shift to light in vivo, and whether this effect could be seen on induction by light of the circadian gene per1. Through measurement of running wheel activity of golden hamsters, access to a new wheel for 3 d was shown to diminish photic phase delays with no effect on phase advances. As seen using in situ hybridization, however, there was no effect on levels of light-induced per1 mRNA. This study indicates a possible role for this paradigm as a model of interactions between photic and nonphotic stimuli.     

自愿转轮运动对雄性长爪沙鼠体重和能量代谢的影响

运动是影响动物能量平衡和体重变化的重要因素之一。为研究自愿转轮运动对体重和能量代谢的影响,我们监测了8 周自愿转轮运动过程中,雄性长爪沙鼠的体重、能量摄入、消化率、静止代谢率(RMR) 和非颤抖性产热(NST)的变化,以及8 周后的体脂含量、血清甲状腺激素(T3、T4 )和瘦素(leptin)水平的变化等。结果发现,8 周自愿转轮运动增加了长爪沙鼠的体重和能量摄入以及血清瘦素水平(血清瘦素浓度比对照组高27% ),但对消化率、RMR 和褐色脂肪组织的线粒体蛋白浓度等没有明显影响。尽管体脂含量和血清T₃ 和T₄ 没有显著差异,但运动组体脂含量比对照组高33%,血清T₃ 和T₄ 水平分别比对照组低10% 和38%。血清瘦素浓度与体脂重量呈正相关。因此,自动转轮运动并没有降低动物的体重和体脂含量,但瘦素和甲状腺素在雄性长爪沙鼠能量代谢和能量平衡中的作用尚需进一步确定。     

A simple field method to identify foot strike pattern during running

Identifying foot strike patterns in running is an important issue for sport clinicians, coaches and footwear industrials. Current methods allow the monitoring of either many steps in laboratory conditions or only a few steps in the field. Because measuring running biomechanics during actual practice is critical, our purpose is to validate a method aiming at identifying foot strike patterns during continuous field measurements. Based on heel and metatarsal accelerations, this method requires two uniaxial accelerometers. The time between heel and metatarsal acceleration peaks (THM) was compared to the foot strike angle in the sagittal plane (α_foot) obtained by 2D video analysis for various conditions of speed, slope, footwear, foot strike and state of fatigue. Acceleration and kinematic measurements were performed at 1000 Hz and 120 Hz, respectively, during 2-min treadmill running bouts. Significant correlations were observed between THM and α_foot for 14 out of 15 conditions. The overall correlation coefficient was r=0.916 (P<0.0001, n=288). The THM method is thus highly reliable for a wide range of speeds and slopes, and for all types of foot strike except for extreme forefoot strike during which the heel rarely or never strikes the ground, and for different footwears and states of fatigue. We proposed a classification based on THM: FFS<−5.49 ms<MFS<15.2 ms<RFS. With only a few precautions being necessary to ensure appropriate use of this method, it is reliable for distinguishing rearfoot and non-rearfoot strikers in situ.     

Modelling bivariate relationships when repeated measurements are recorded on more than one subject

This paper examines the problems of modelling bivariate relationships when repeated observations are recorded for each subject. The statistical methods required to test for a common group model were introduced using an example from exercise physiology, where the oxygen cost of running at four different speeds was recorded for a group of 30 recreationally active males. When data for each subject were studied individually, both the plots and correlations suggested the relationship to be linear. Hence, the homogeneity of the subjects' regression lines was compared using the appropriate ANOVA test. The analysis revealed a significant difference in the slopes and intercepts of the lines, thus precluding the use of a single linear model to represent the group. If the subjects were divided into two groups according to the median maximum oxygen consumption (VO2max), a multivariate analysis of variance of the slope and intercept parameters helped to explain some of this heterogeneity (P less than 0.05). However, for physiological rather than statistical reasons, it was necessary to re-analyse the data without the fourth running speed. The revised analysis suggested that the subjects' lines would be better modelled with a common slope but separate intercepts. As before, by dividing the subjects into two groups according to the median VO2max score, a simple t-test indicated that differences in the subjects' intercept parameters were not significant (P = 0.08). Notwithstanding the relatively homogeneous nature of the 30 subjects in terms of VO2max, the statistical methods showed that differences in running economy are, to some extent, dependent on VO2max.     

Accuracy of non-differential GPS for the determination of speed over ground

Accurate determination of speed is important in many studies of human and animal locomotion. Some global positioning system (GPS) receivers can data log instantaneous speed. The speed accuracy of these systems is, however, unclear with manufacturers reporting velocity accuracies of 0.1–0.2 ms⁻¹. This study set out to trial non-differential GPS as a means of determining speed under real-life conditions.

A bicycle was ridden around a running track and a custom-made bicycle speedometer was calibrated. Additional experiments were performed around circular tracks of known circumference and along a straight road. Instantaneous speed was determined simultaneously by the custom speedometer and a data logging helmet-mounted GPS receiver. GPS speed was compared to speedometer speed. The effect on speed accuracy of satellite number; changing satellite geometry, achieved through shielding the GPS antenna; speed; horizontal dilution of precision and cyclist position on a straight or a bend, was evaluated. The relative contribution of each variable to overall speed accuracy was determined by ANOVA. The speed determined by the GPS receiver was within 0.2 ms⁻¹ of the true speed measured for 45% of the values with a further 19% lying within 0.4 ms⁻¹ (n=5060). The accuracy of speed determination was preserved even when the positional data were degraded due to poor satellite number or geometry. GPS data loggers are therefore accurate for the determination of speed over-ground in biomechanical and energetic studies performed on relatively straight courses. Errors increase on circular paths, especially those with small radii of curvature, due to a tendency to underestimate speed.