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991.
Question: Understanding the mechanisms underlying how habitat degradation, topography and rainfall variability interactively affect seed distribution and seedling recruitment is crucial for explaining plant community patterns and dynamics. Interactions between these major factors were studied together in a semiarid sand dune grassland. Location: Eastern Inner Mongolia, China. Methods: The study system used four sites of fixed, semifixed, semishifting and shifting sand dune grasslands, representing a gradient of habitat degradation. We investigated the density of germinable seeds deposited in the top 5 cm of soil and in situ seedling emergence (number of seedlings emerging early in the growing season) and establishment (number of plants recruited at the end of the growing season) at three topographic positions (dune top, windward and leeward sides) within each site over 2 years that differed in rainfall. Habitat characteristics (i.e. vegetation cover, plant species composition and diversity, soil moisture and nutrient availability and soil erodibility) of the four sites were also measured. Results: Habitat degradation (i.e. decreased vegetation cover and enhanced wind erosion rate) significantly reduced the size of the germinable soil seed bank. On average, germinable seed number from the high‐vegetation cover fixed dune was 36‐fold larger than the low‐vegetation cover shifting dune, and eight‐ and two‐fold larger, respectively, than the semishifting and semifixed dunes with intermediate vegetation cover. We observed within‐habitat variability in seed distribution, but among‐topographic position variation differed among habitats. Seedling recruitment showed large between‐year, and among‐ and within‐habitat variability, but these variations varied significantly depending on the response variables evaluated (i.e. initial seedling density, final plant density, emergence rate and recruitment rate). Path analysis revealed complex density‐dependent positive and negative, direct and indirect effects of germinable seed density and initial seedling density on recruitment, but the relative importance of these density‐dependent effects varied depending on habitat type and rainfall availability. Conclusion: Our results suggest that habitat degradation, microtopography and rainfall availability interact in shaping sand dune seed bank and plant community recruitment patterns and dynamics. Their effects were mainly mediated through changes in both the biotic and abiotic environment during the process of habitat deterioration. 相似文献
992.
Sawako Tokuda Tsutomu Hattori Yu-Cheng Dai Yuko Ota Peter K. Buchanan 《Mycoscience》2009,50(3):190-202
993.
Aims: To determine Legionella pollution prevalence, describe the amount of Legionellae with respect to temperature in Shanghai cooling tower water (CTWs) in various types of public sites.
Methods and Results: Six urban districts were selected as the study fields, adopting multiple-phase sampling methods. Routine culture was used to identify Legionellae. Of the samples, 58·9% (189/321) were observed to be positive, 19·9% were isolated over 100 CFU ml−1 . Legionella pneumophila serogroup 1 was the most frequently isolated species (155/189, 82·0%), followed by Leg. micdadei that was at the second place (44/189, 23·3%). The mean CFU ml−1 of Legionellae in CTWs reached its peak from July to September. Over all 15·4% of the samples exceeding 100 CFU ml−1 were observed in a hospital setting.
Conclusions: The prevalence of Legionella pollution in CTWs, especially in CTWs of subway stations and hospitals, is worrying, and the positive rate and CFU ml−1 of Legionellae in CTWs have a close relationship with air temperature.
Significance and Impact of the Study: The study demonstrates pollution prevalence rates in different types of sites and various seasons, and provides a proportion of different serogroups of Legionellae . It illuminates an urgent need for dealing with the potential risk of legionellosis in Shanghai, through improved control and prevention strategies. 相似文献
Methods and Results: Six urban districts were selected as the study fields, adopting multiple-phase sampling methods. Routine culture was used to identify Legionellae. Of the samples, 58·9% (189/321) were observed to be positive, 19·9% were isolated over 100 CFU ml
Conclusions: The prevalence of Legionella pollution in CTWs, especially in CTWs of subway stations and hospitals, is worrying, and the positive rate and CFU ml
Significance and Impact of the Study: The study demonstrates pollution prevalence rates in different types of sites and various seasons, and provides a proportion of different serogroups of Legionellae . It illuminates an urgent need for dealing with the potential risk of legionellosis in Shanghai, through improved control and prevention strategies. 相似文献
994.
The biological control efficacy of single or multiple applications of the mutualistic endophyte Fusarium oxysporum strain 162, the egg pathogen Paecilomyces lilacinus strain 251 and the antagonistic bacteria Bacillus firmus toward Radopholus similis was investigated in pot trials with banana under glasshouse conditions. R. similis was controlled substantially in single and combined applications of F. oxysporum with P. lilacinus or B. firmus. The combination of F. oxysporum and P. lilacinus caused a 68.5% reduction in nematode density whereas the individual applications reduced the density by 27.8% and 54.8% over
the controls, respectively. Combined application of F. oxysporum and B. firmus was the most effective treatment in controlling R. similis on banana (86.2%), followed by B. firmus alone (63.7%). The compatibility of the biocontrol agents, as well the capacity of F. oxysporum to colonize banana roots in the absence or presence of P. lilacinus was also investigated. P. lilacinus did not adversely affect endophytic colonization by F. oxysporum. Biological control of R. similis in banana can therefore be enhanced via combined applications of antagonists with different modes of action that target different
stages in the infection process.
Handling editor: Ralf-Udo Ehlers 相似文献
995.
Twenty-five biotically pollinated plants of the Chaco Serrano Forest (Córdoba, Argentina) were studied in order to analyze whether ‘flower functionality’ is related to the relationship between pollen size and pistil length. Because flower functionality may act on the respective mean values of pollen size and pistil length rather than on intraspecific variation in these traits, we expected (1) a high positive correlation between pollen size and pistil length in a set of sympatric species, independent of their degree of pollination specialization or generalization; and (2) no interspecific correlation between the coefficients of variation (CVs) of those traits. On the other hand, on the assumption that pollinators are influencing the variation in floral traits (e.g. in pistil length) we expected lower mean phenotypic variation of pollen size and pistil length in pollination-specialist plants than in pollination-generalist ones. A positive correlation between pollen size and pistil length was found for the set of species, but not between the CVs of these traits. This trend was maintained when pollination-specialist plants were analyzed separately, but no statistical significance was obtained for the correlation in pollination-generalist plants. Contrary to our expectations, pollination-specialist plants did not show less mean intraspecific variation in floral traits than pollination-generalist plants. Therefore, the relationship between pollen size and pistil length among species suggests that the pollination system may be of less importance as a selective force than flower functionality. 相似文献
996.
REMINGTON J. MOLL JOSHUA J. MILLSPAUGH JEFF BERINGER JOEL SARTWELL RAMI J. WOODS KURT C. VERCAUTEREN 《The Journal of wildlife management》2009,73(4):609-614
ABSTRACT Animal-borne video and environmental data collection systems (AVEDs) are an advanced form of biotelemetry that combines video with other sensors. As a proxy for physiological stress, we assessed fecal glucocorticoid metabolite (FGM) excretion in 7 white-tailed deer (Odocoileus virginianus) fitted with AVED dummy collars; 9 additional deer served as controls. We collected fecal samples over 3 2-week periods: pretreatment, treatment, and posttreatment periods. There were no differences in FGMs across time periods (F2,218 = 1.94, P = 0.147) and no difference between FGMs of control and treatment individuals (F1,14 = 0.72, P = 0.411). Fecal glucocorticoid metabolite excretion in AVED-collared deer was indistinguishable from uncollared animals and within the normal, baseline range for this species. Absence of an adrenal response to collaring suggested that AVED collaring does not induce physiological stress in deer. 相似文献
997.
Werner Sandmann 《Mathematical biosciences》2009,221(1):43-2141
Stochastic simulation of biological systems proceeds by repeatedly generating sample paths or trajectories of the underlying stochastic process, from which many relevant and important system properties can be obtained. While a great deal of research is targeted towards accelerated trajectory generation, issues concerned with the variability across trajectories are often neglected. Advanced methods for properly quantifying the statistical accuracy and determining a reasonable number of trajectories are hardly addressed formally in the context of biological system simulation, though mathematical statistics provides a large body of powerful theory. We invoke this theory and show how mathematically well-founded sequential estimation approaches serve for systematically generating enough but not too many trajectories for achieving a certain prescribed accuracy. The practical applicability is demonstrated and illustrated by numerical examples through simulation studies of an immigration-death process and a gene regulatory network. 相似文献
998.
C. S. Silva E. Bovarotti M. I. Rodrigues C. O. Hokka Marlei Barboza 《Bioprocess and biosystems engineering》2009,32(5):625-632
The purification of clavulanic acid (CA), which is an important β-lactam antibiotic produced by submerged cultivation of Streptomyces clavuligerus, was studied through the use of phosphate and polyethylene glycol-based aqueous two-phase systems. The parameters’ effect
on the yield and purification was evaluated through an experimental design and the preliminary results showed that the polyethylene
molecular mass and tie-line length and phase volume ratio exerted the strongest effect on the yield and distribution coefficient
in the range tested. In addition, the response surface methodology was used to optimize the distribution coefficient, yield,
and purification factor. The optimal conditions of yield and purification factor are in the regions where polyethylene has
a low molecular mass, pH close to the isoelectric point, and lower top phase volume. A 100% yield and a 1.5-fold purification
factor are obtained when extracting CA by maximizing the conditions of an aqueous two-phase system. 相似文献
999.
Microorganisms have been the main sources for the production of chemicals. Production of chemicals requires the development of low-cost and higher-yield processes. Towards this goal, microbial strains with higher levels of production should be first considered. Metabolic engineering has been used extensively over the past two to three decades to increase production of these chemicals. Advances in omics technology and computational simulation are allowing us to perform metabolic engineering at the systems level. By combining the results of omics analyses and computational simulation, systems biology allows us to understand cellular physiology and characteristics, which can subsequently be used for designing strategies. Here, we review the current status of metabolic engineering based on systems biology for chemical production and discuss future prospects. 相似文献
1000.
Bryan W Miller Garnet Lau Chris Grouios Emanuela Mollica Miriam Barrios‐Rodiles Yongmei Liu Alessandro Datti Quaid Morris Jeffrey L Wrana Liliana Attisano 《Molecular systems biology》2009,5(1)
Large‐scale proteomic approaches have been used to study signaling pathways. However, identification of biologically relevant hits from a single screen remains challenging due to limitations inherent in each individual approach. To overcome these limitations, we implemented an integrated, multi‐dimensional approach and used it to identify Wnt pathway modulators. The LUMIER protein–protein interaction mapping method was used in conjunction with two functional screens that examined the effect of overexpression and siRNA‐mediated gene knockdown on Wnt signaling. Meta‐analysis of the three data sets yielded a combined pathway score (CPS) for each tested component, a value reflecting the likelihood that an individual protein is a Wnt pathway regulator. We characterized the role of two proteins with high CPSs, Ube2m and Nkd1. We show that Ube2m interacts with and modulates β‐catenin stability, and that the antagonistic effect of Nkd1 on Wnt signaling requires interaction with Axin, itself a negative pathway regulator. Thus, integrated physical and functional mapping in mammalian cells can identify signaling components with high confidence and provides unanticipated insights into pathway regulators. 相似文献