首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   323篇
  免费   18篇
  国内免费   22篇
  2023年   3篇
  2022年   3篇
  2021年   8篇
  2020年   7篇
  2019年   15篇
  2018年   7篇
  2017年   9篇
  2016年   9篇
  2015年   10篇
  2014年   11篇
  2013年   10篇
  2012年   7篇
  2011年   9篇
  2010年   13篇
  2009年   17篇
  2008年   14篇
  2007年   13篇
  2006年   18篇
  2005年   8篇
  2004年   7篇
  2003年   7篇
  2002年   9篇
  2001年   5篇
  2000年   6篇
  1999年   3篇
  1998年   7篇
  1997年   4篇
  1996年   11篇
  1995年   7篇
  1994年   6篇
  1993年   6篇
  1992年   6篇
  1991年   11篇
  1990年   7篇
  1989年   3篇
  1988年   6篇
  1987年   2篇
  1986年   4篇
  1985年   8篇
  1984年   6篇
  1983年   2篇
  1982年   5篇
  1981年   7篇
  1980年   6篇
  1979年   3篇
  1978年   3篇
  1977年   6篇
  1976年   2篇
  1974年   2篇
  1973年   3篇
排序方式: 共有363条查询结果,搜索用时 15 毫秒
81.
The extracellular concentrations of 5-hydroxytryptamine (5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) have been determined in six brain areas of awake rats (frontal cortex, striatum, hypothalamus, hippocampus, inferior colliculus, and raphe nuclei) using intracerebral microdialysis. The extracellular levels of 5-HT showed no significant differences among the brain regions studied. The tissue levels of 5-HT and 5-HIAA as well as the extracellular concentration of 5-HIAA were significantly higher in raphe nuclei. The regional distribution of tissue and extracellular 5-HIAA were very similar, suggesting that extracellular 5-HIAA depends mainly on the output from the intracellular compartment. On the other hand, extracellular 5-HT and tissue 5-HT showed a different distribution pattern. The tissue/extracellular ratio for 5-HT ranged from 739 in frontal cortex to 2,882 in raphe, whereas it only amounted to 1.8-3.6 for 5-HIAA. The relationship between the present results and the density of 5-HT uptake sites in these areas is discussed.  相似文献   
82.
本文研究了鄂西木林子种子植物区系的地理分布,并探讨了川鄂山地的区域性特征。木林子区系现知种子植物共134科、488属、约1043种(包括种下分类单位,下同)。本区系10个东亚特有科中有6科的分布边界止于川鄂山地,3科盛产于川鄂山地。约141属的分布区明显集中于川鄂山地或以川鄂山地为其主要分布区之一,约占该区系488属的28.9%。有52属的分布边界限于川鄂山地,另有27个百种以上大属的分布在川鄂山地呈急剧减少的态势。本区系以川鄂山地为主要分布区的有131种,占全部种数的12.56%,其中仅分布于川鄂山地的就有36种;还有约270种的分布边界止于川鄂山地。川鄂山地独特生境的阻限和对植物种系发生的诱发作用,是形成这种地理分布格局的重要原因。  相似文献   
83.
本文结合采用扫描和透射电子显微镜(包括冷冻断裂-蚀刻、超薄切片以及细胞化学染色法),研究了金鱼精子的超微结构特征,结果表明金鱼精子的质膜和核膜都具有区域特异性:1)精子质膜内大部分区域含有许多蛋白颗粒,但在特定区域内,蛋白颗粒呈有序排列,构成晶格状结构。2)精子头颈部和尾部均有液泡密集之处,凡是覆盖着液泡区的质膜内,几乎都不含有蛋白颗粒。3)液泡区能被细胞化学方法染成致密色,表明内含糖蛋白。4)核膜孔只集中存在于靠近颈部的核膜上,而其他部分则没有。本文对上述诸点进行了讨论。  相似文献   
84.
85.
86.
Recent genome-wide association studies have identified various dyslipidemia-related genetic variants. However, most studies were conducted in a cross-sectional manner. We thus performed longitudinal exome-wide association studies of dyslipidemia in a Japanese population. We used ~244,000 genetic variants and clinical data of 6022 Japanese individuals who had undergone annual health checkups for several years. After quality control, the association of dyslipidemia-related phenotypes with 24,691 single nucleotide polymorphisms (SNPs) was tested using the generalized estimating equation model. In total, 82 SNPs were significantly (P < 2.03 × 10?6) associated with dyslipidemia phenotypes. Of these SNPs, four (rs74416240 of TCHP, rs925368 of GIT2, rs7969300 of ATXN2, and rs12231744 of NAA25) and two (rs34902660 of SLC17A3 and rs1042127 of CDSN) were identified as novel genetic determinants of hypo-HDL- and hyper-LDL-cholesterolemia, respectively. A replication study using the cross-sectional data of 8310 Japanese individuals showed the association of the six identified SNPs with dyslipidemia-related traits.  相似文献   
87.
In regions with thousands of lakes, large scale regional macrophyte surveys are rarely done due to logistical difficulties and high costs. We examined whether remote sensing can be used for regional monitoring of macrophytes in inland lakes using a field study of 13 lakes in Michigan, USA (nine model development lakes and four model testing lakes). Our objectives were: (1) to determine if different levels of macrophyte cover, different growth forms or specific species could be detected using the Landsat-5 TM sensor, and (2) to determine if we could improve predictions of macrophyte abundance and distribution in lakes by including sediment type or measures of water clarity (Secchi disk transparency, chlorophyll a, phytoplankton biovolume, or water color) in our models. Using binomial and multinomial logistic regression models, we found statistically significant relationships between most macrophyte measures and Landsat-5 TM values in the nine model development lakes (percent concordant values: 58–97%). Additionally, we found significant correlations between three lake characteristics and the TM values within lake pelagic zones, despite the inability of these variables to improve model predictions. However, model validation using four lakes was generally low, suggesting caution in applying these models to other lakes. Although the initial model development results suggest that remote sensing is a potentially promising tool for regionally assessing macrophytes, more research is necessary to refine the models in order for them to be applied to unsampled lakes.  相似文献   
88.
Regional analyses and biogeochemical models predict that ecosystem N pools and N cycling rates must increase from the semi-arid shortgrass steppe to the sub-humid tallgrass prairie of the Central Great Plains, yet few field data exist to evaluate these predictions. In this paper, we measured rates of net N mineralization, N in above- and belowground primary production, total soil organic matter N pools, soil inorganic N pools and capture in resin bags, decomposition rates, foliar 15N, and N use efficiency (NUE) across a precipitation gradient. We found that net N mineralization did not increase across the gradient, despite more N generally being found in plant production, suggesting higher N uptake, in the wetter areas. NUE of plants increased with precipitation, and δ15N foliar values and resin-captured N in soils decreased, all of which are consistent with the hypothesis that N cycling is tighter at the wet end of the gradient. Litter decomposition appeared to play a role in maintaining this regional N cycling trend: litter decomposed more slowly and released less N at the wet end of the gradient. These results suggest that immobilization of N within the plant–soil system increases from semi-arid shortgrass steppe to sub-humid tallgrass prairie. Despite the fact that N pools increase along a bio-climatic gradient from shortgrass steppe to mixed grass and tallgrass prairie, this element becomes relatively more limiting and is therefore more tightly conserved at the wettest end of the gradient. Similar to findings from forested systems, our results suggest that grassland N cycling becomes more open to N loss with increasing aridity.  相似文献   
89.
Promoter hypermethylation-associated tumor suppressor gene (TSG) silencing has been explored as a therapeutic target for hypomethylating agents. Promoter methylation change may serve as a pharmacodynamic endpoint for evaluation of the efficacy of these agents and predict the patient’s clinical response. Here a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been developed for quantitative regional DNA methylation analysis using the molar ratio of 5-methyl-2′-deoxycytidine (5mdC) to 2′-deoxycytidine (2dC) in the enzymatic hydrolysate of fully methylated bisulfite-converted polymerase chain reaction (PCR) amplicons as the methylation indicator. The assay can differentiate 5% of promoter methylation level with an intraday precision ranging from 3 to 16% using two TSGs: HIN-1 and RASSF1A. This method was applied to characterize decitabine-induced promoter DNA methylation changes of these two TSGs in a breast cancer MCF-7 cell line. Promoter methylation of these TSGs was found to decrease in a dose-dependent manner. Correspondingly, the expression of these TSGs was enhanced. The sensitivity and reproducibility of the method make it a valuable tool for specific gene methylation analysis that could aid characterization of hypomethylating activity on specific genes by hypomethylating agents in a clinical setting.  相似文献   
90.
We describe genetic screens, molecular methods and web resources newly available to utilize Dissociation (Ds) as an insertional mutagen in maize. Over 1700 Ds elements have been distributed throughout the maize genome to serve as donor elements for local or regional mutagenesis. Two genetic screens are described to identify Ds insertions in genes-of-interest (goi). In scheme I, Ds is used to generate insertion alleles when a recessive reference allele is available. A Ds insertion will enable the cloning of the target gene and can be used to create an allelic series. In scheme II, Ds insertions in a goi are identified using a PCR-based screen to identify the rare insertion alleles among a population of testcross progeny. We detail an inverse PCR protocol to rapidly amplify sequences flanking Ds insertion alleles and describe a high-throughput 96-well plate-based DNA extraction method for the recovery of high-quality genomic DNA from seedling tissues. We also describe several web-based tools for browsing, searching and accessing the genetic materials described. The development of these Ds insertion lines promises to greatly accelerate functional genomics studies in maize.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号