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排序方式: 共有396条查询结果,搜索用时 15 毫秒
21.
Rytis Prekeris Judith Klumperman Yu A. Chen Richard H. Scheller 《The Journal of cell biology》1998,143(4):957-971
Endocytosis-mediated recycling of plasma membrane is a critical vesicle trafficking step important in diverse biological processes. The membrane trafficking decisions and sorting events take place in a series of heterogeneous and highly dynamic organelles, the endosomes. Syntaxin 13, a recently discovered member of the syntaxin family, has been suggested to play a role in mediating endosomal trafficking. To better understand the function of syntaxin 13 we examined its intracellular distribution in nonpolarized cells. By confocal immunofluorescence and electron microscopy, syntaxin 13 is primarily found in tubular early and recycling endosomes, where it colocalizes with transferrin receptor. Additional labeling is also present in endosomal vacuoles, where it is often found in clathrin-coated membrane areas. Furthermore, anti-syntaxin 13 antibody inhibits transferrin receptor recycling in permeabilized PC12 cells. Immunoprecipitation of syntaxin 13 revealed that, in Triton X-100 extracts, syntaxin 13 is present in a complex(es) comprised of βSNAP, VAMP 2/3, and SNAP-25. This complex(es) binds exogenously added αSNAP and NSF and dissociates in the presence of ATP, but not ATPγS. These results support a role for syntaxin 13 in membrane fusion events during the recycling of plasma membrane proteins. 相似文献
22.
Endosomal vacuoles of the prepupal salivary glands of Drosophila play an essential role in the metabolic reallocation of iron 下载免费PDF全文
Robert Farkaš Denisa Beňová‐Liszeková Lucia Mentelová Milan Beňo Klaudia Babišová Ludmila Trusinová‐Pečeňová Otakar Raška Bruce A. Chase Ivan Raška 《Development, growth & differentiation》2018,60(7):411-430
In the recent past, we demonstrated that a great deal is going on in the salivary glands of Drosophila in the interval after they release their glycoprotein‐rich secretory glue during pupariation. The early‐to‐mid prepupal salivary glands undergo extensive endocytosis with widespread vacuolation of the cytoplasm followed by massive apocrine secretion. Here, we describe additional novel properties of these endosomes. The use of vital pH‐sensitive probes provided confirmatory evidence that these endosomes have acidic contents and that there are two types of endocytosis seen in the prepupal glands. The salivary glands simultaneously generate mildly acidic, small, basally‐derived endosomes and strongly acidic, large and apical endosomes. Staining of the large vacuoles with vital acidic probes is possible only after there is ambipolar fusion of both basal and apical endosomes, since only basally‐derived endosomes can bring fluorescent probes into the vesicular system. We obtained multiple lines of evidence that the small basally‐derived endosomes are chiefly involved in the uptake of dietary Fe3+ iron. The fusion of basal endosomes with the larger and strongly acidic apical endosomes appears to facilitate optimal conditions for ferrireductase activity inside the vacuoles to release metabolic Fe2+ iron. While iron was not detectable directly due to limited staining sensitivity, we found increasing fluorescence of the glutathione‐sensitive probe CellTracker Blue CMAC in large vacuoles, which appeared to depend on the amount of iron released by ferrireductase. Moreover, heterologous fluorescently‐labeled mammalian iron‐bound transferrin is actively taken up, providing direct evidence for active iron uptake by basal endocytosis. In addition, we serendipitously found that small (basal) endosomes were uniquely recognized by PNA lectin, whereas large (apical) vacuoles bound DBA lectin. 相似文献
23.
《Autophagy》2013,9(10):1639-1641
The role of membrane remodeling and phosphoinositide-binding proteins in autophagy remains elusive. PX domain proteins bind phosphoinositides and participate in membrane remodeling and trafficking events and we therefore hypothesized that one or several PX domain proteins are involved in autophagy. Indeed, the PX-BAR protein SNX18 was identified as a positive regulator of autophagosome formation using an image-based siRNA screen. We show that SNX18 interacts with ATG16L1 and LC3, and functions downstream of ATG14 and the class III PtdIns3K complex in autophagosome formation. SNX18 facilitates recruitment of ATG16L1 to perinuclear recycling endosomes, and its overexpression leads to tubulation of ATG16L1- and LC3-positive membranes. We propose that SNX18 promotes LC3 lipidation and tubulation of recycling endosomes to provide membrane for phagophore expansion. 相似文献
24.
We found that wortmannin, a potent phosphoinositide 3-kinase (PI3K) inhibitor, markedly induced the formation of Rab21-positive tubular compartments in A431 cells. By time-lapse fluorescence microscopy of live cells co-expressing fluorescent protein-fused Rab21 and other marker proteins, it was shown that the Rab21-positive tubules in wortmannin-treated cells were derived from Rab5-positive early endosomes, but not from late endosomes, recycling endosomes, lysosomes or the trans-Golgi network. The formation of Rab21-positive tubules was very dynamic and required microtubules. Rab21-positive tubules were also formed by the treatment of cells with 3-methyladenine (3-MA), which inhibits class III PI3K rather than class I PI3K. Furthermore, the loss of PI(3)P correlated with the tubulation of Rab21-positive endosomes in cells co-expressing fluorescent protein-fused Rab21 and a tandem FYVE domain. These results suggest that the lowering of PI(3)P as a result of class III PI3K inhibition may be an important cue for the morphological change of Rab21-positive early endosomes from vesicular to tubular form. 相似文献
25.
Carles M. Gasol Ramon Farreny Xavier Gabarrell Joan Rieradevall 《The International Journal of Life Cycle Assessment》2008,13(5):421-431
Goal, scope and background The industrial packages sector has great importance for the transport sector in Europe. These containers, mainly wooden pallets
and spools, are subject to European legislation, which promotes their reuse and recycling. This study uses life cycle assessment
(LCA) to assess the environmental impact of the current management system in this sector and the benefits and drawbacks of
different reuse intensities as a waste prevention strategy as opposed to the recycling option.
Materials and methods In this paper, four case studies located in Spain and representative of the wooden package sector in Europe are analysed:
high reuse pallet, low reuse pallet, low reuse spool and null reuse spool. For the LCA study cases, the functional unit is
that required to satisfy the transport necessity of 1,000 t by road. The impact and energy consumption assessment methods
used are CML 2 Baseline 2000 and Cumulative Energy Demand. Data are mostly provided by the leading enterprises and organisations
in this sector.
Results The paper provides, as a first result, a comprehensive inventory of the systems under study. Secondly, our assessment shows
that the systems with higher reuse intensity show a reduction in energy and wood consumption and all the environmental impact
categories except for the global warming potential from 34.0% to 81.0% in the pallet study cases and from 50.4% to 72.8% in
the spool ones. This reduction is at the expense of the maintenance stage, which on the contrary increases its impact, although
it is still relatively small—less than 7% in all the impact categories and flow indicators of the study cases. The highest
impact stages are transport, raw material extraction and the process chain. The final disposal and maintenance stages are
the lowest impact, contributing at most to less than 30% of the impact in the pallet study cases and 10% in the spool cases.
Discussion Wood consumption (WC), directly related to the number of containers needed to satisfy the functional unit, is the main factor
in determining the impact of the stages, especially in the raw materials extraction and process chain stages, assuming that
these are undertaken with the same technologies in all the case studies. Other variables, such as the management system, the
maintenance index and the final disposal scenario, affect the impact of the remaining stages: transport, maintenance and final
disposal. The global warming potential results obtained demonstrate the environmental benefits of using containers made of
a renewable resource such as wood instead of using other materials, but these results are not expected to prioritise the lower
reuse systems because of their better performance in this category.
Conclusions Reuse, a strategy capable of reducing the environmental impacts of the wooden container systems, is preferable to recycling,
while the package maintenance tasks are still feasible. Therefore, reuse, combined with recycling as final disposal, should
be encouraged to reduce the demand for natural resources and the waste generated.
Recommendations Based on these results, attention should be paid to the maintenance stage, which, being the lowest-impact one, could substantially
reduce the impact of the remaining stages. 相似文献
26.
Short-term (2–30 min) cyclic stretch activates the Na pump in cultured aortic smooth muscle cells (ASMCs). This effect of
stretch involves the phosphotidylinositol 3-kinase (PI 3-kinase) participation. Presently, we investigated whether this stimulation
is the result of translocation of Na+,K+-ATPase from endosomes to the plasma membrane. ASMCs were stretched 20% for 5 min using the Flexercell Strain Unit. The plasma
membrane and endosome fractions were isolated and Western blotted to localize the Na+,K+-ATPase α-1-subunit protein. Membrane marker enzyme, 5′ nucleotidase activity, and the early and recycling endosome markers
Rab4 and Rab11 were used to verify the enrichment of these fractions. Stretch increased Na+,K+-ATPase α-1 expression in plasma membrane fractions and decreased it in endosomes. PI 3-kinase inhibitors LY294002 and wortmannin
blocked the stretch-induced translocation of the Na+,K+-ATPase α-1-subunit. Rab4 and Rab11 were enriched in the endosomal fraction, whereas 5′ nucleotidase activity was enriched
in the plasma membrane fraction. We conclude that stimulation of the Na pump activity by shortterm cyclic stretch is the result,
at least in part, of transport of the α-subunit of the enzyme from endosomes to the plasma membrane. 相似文献
27.
Eutrophication is a world-wide environmental issue. Lake Taihu is a typical large, shallow, eutrophic lake located in delta of River Changjiang (Yangtze River). A large-scale ecological engineering experiment targeted at water quality improvement was implemented in Meiliang Bay, Lake Taihu. In this special issue, there are six papers related to water purification and algal bloom control techniques applied in this experiment. Four papers address the validity and efficiency of water quality improvement of this ecological engineering and one paper presents a similar but small-size ecological engineering. The others focus on macrophyte restoration, aquatic plant management and recycling exploitation. The editorial paper highlights the main results and conclusions from these papers. 相似文献
28.
Mechanical biological treatment (MBT) of mixed waste streams is becoming increasingly popular as a method for treating municipal solid waste (MSW). Whilst this process can separate many recyclates from mixed waste, the resultant organic residue can contain high levels of heavy metals and physical and biological contaminants. This review assesses the potential end uses and sustainable markets for this organic residue. Critical evaluation reveals that the best option for using this organic resource is in land remediation and restoration schemes. For example, application of MSW-derived composts at acidic heavy metal contaminated sites has ameliorated soil pollution with minimal risk. We conclude that although MSW-derived composts are of low value, they still represent a valuable resource particularly for use in post-industrial environments. A holistic view should be taken when regulating the use of such composts, taking into account the specific situation of application and the environmental pitfalls of alternative disposal routes. 相似文献
29.
RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells 总被引:1,自引:0,他引:1
Rondanino C Rojas R Ruiz WG Wang E Hughey RP Dunn KW Apodaca G 《Traffic (Copenhagen, Denmark)》2007,8(7):932-949
The Rho family of GTPases is implicated in the control of endocytic and biosynthetic traffic of many cell types; however, the cellular distribution of RhoB remains controversial and its function is not well understood. Using confocal microscopy, we found that endogenous RhoB and green fluorescent protein-tagged wild-type RhoB were localized to early endosomes, and to a much lesser extent to recycling endosomes, late endosomes or Golgi complex of fixed or live polarized Madin-Darby canine kidney cells. Consistent with RhoB localization to early endosomes, we observed that expression of dominant-negative RhoBN19 or dominant-active RhoBV14 altered postendocytic traffic of ligand-receptor complexes that undergo recycling, degradation or transcytosis. In vitro assays established that RhoB modulated the basolateral-to-apical transcytotic pathway by regulating cargo exit from basolateral early endosomes. Our results indicate that RhoB is localized, in part, to early endosomes where it regulates receptor egress through the early endocytic system. 相似文献
30.
Kangmin He Xiaohua Yan Nan Li Song Dang Li Xu Bing Zhao Zijian Li Zhizhen Lv Xiaohong Fang Youyi Zhang Ye-Guang Chen 《Cell research》2015,25(6):738-752
Endocytosis and intracellular sorting of transforming growth factor-β (TGF-β) receptors play an important regulatory role in TGF-β signaling. Two major endocytic pathways, clathrin- and caveolae-mediated endocytosis, have been reported to independently mediate the internalization of TGF-β receptors. In this study, we demonstrate that the clathrin- and caveolae-mediated endocytic pathways can converge during TGF-β receptor endocytic trafficking. By tracking the intracellular dynamics of fluorescently-labeled TGF-β type I receptor (TβRI), we found that after mediating TβRI internalization, certain clathrin-coated vesicles and caveolar vesicles are fused underneath the plasma membrane, forming a novel type of caveolin-1 and clathrin double-positive vesicles. Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized TβRI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes). We further showed that the caveolin-1-positive early endosomes are positive for Smad3/SARA, Rab11 and Smad7/Smurf2, and may act as a multifunctional device for TGF-β signaling and TGF-β receptor recycling and degradation. Therefore, these findings uncover a novel scenario of endocytosis, the direct fusion of clathrin-coated and caveolae vesicles during TGF-β receptor endocytic trafficking, which leads to the formation of the multifunctional sorting device, caveolin-1-positive early endosomes, for TGF-β receptors. 相似文献