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61.
Akira Isogai Akinori Suzuki Shizuo Higashikawa Shimpei Kuyama Saburo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(4):1023-1024
The structure of the xyloglucan synthesised in vitro by the particulate fraction of suspension-cultured soybean (Glycine max) cells from UDP-glucose and UDP-xylose is mainly composed of two kinds of oligosaccharide-building blocks, a heptasaccharide unit and a pentassaccharide unit [T. Hayashi and K. Matsuda, J. Biol Chem., 256, 11117 (1981)]. The synthesis of the pentasaccharide unit is probably the first step in the construction of oligosaccharide building blocks to elongate the ²-1,4-glucan backbone. This enzymatically synthesized xyloglucan was shown to have the same molecular size (Mw, 180,000) as the xyloglucan prepared from soybean cell walls by gel filtration on a Sepharose CL-6B column, and the same building blocks distributed among each fraction. A pulse-chase experiment indicated that the pentasaccharide unit was converted into the heptasaccharide unit. The conversion was regulated by the concentration of UDP-xylose. 相似文献
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63.
南京地区12种常绿阔叶树种冬季抗寒性动态变化 总被引:11,自引:0,他引:11
选取南京地区露地引种的12种常绿阔叶树种,借助电导法对其抗寒性进行研究,结果表明:12种常绿树种在2003年与2005年冬季期间,抗寒能力高低排序具有很好的一致性与重复性.12种常绿树种在2005年10月至2006年3月期间随冬季低温诱导进行其抗寒能力呈逐渐增强趋势,其中在1月中旬树种抗寒能力均达到最强,而通常在10月份或3月份期间树种抗寒能力最弱.比较而言,12种常绿阔叶树种对冬季低温条件响应的快慢程度各有差异,其中樟科的香樟(Cinnamomum camphora)、浙江樟(Cinnamomum japonicum)属于低温快速响应型,而冬青科的冬青(Ilex purpurea)、大叶冬青(Ilex latifolia)属于低温滞缓响应型,其余树种为正常响应型.12种常绿阔叶树种试验所得抗寒能力结果与引种地冬季观察结果具有很好的对应性.研究结果可为常绿阔叶树种进一步向较高纬度地区的引种与栽培管理工作提供重要的参考. 相似文献
64.
《Bioscience, biotechnology, and biochemistry》2013,77(6):1135-1140
Recently, high-throughput screening (HTS) has become the mainstream technique for drug discovery. Compounds that are synthesized by combinatorial chemistry might be more suitable than natural products to apply to HTS, because the purification procedure is a drawback of using natural products. Nevertheless, natural products remain an extremely important source of drugs. To overcome the demerits of natural products, we are constructing the RIKEN Natural Products Depository (NPDepo) that is focused primarily on microbial metabolites. In this review, I describe (i) engineering pathways for biosynthetic gene clusters of microbial metabolites, (ii) construction of fraction libraries of microbial metabolites, and (iii) the development of a new screening system using a chemical array and a protein library produced by GLORIA. 相似文献
65.
Styliani Consta Anatoly Malevanets Myong In Oh Mahmoud Sharawy 《Molecular simulation》2018,44(13-14):1033-1043
AbstractThe free energy calculation method emerges as a viable technique for ‘in-silico’ calorimetry. Efficient sampling techniques and the good choice of a reaction path connecting the reactant and the product state enable accurate computations of the free energy differences. We argue that in many cases the thermodynamic integration technique has the lowest variance when the transformation between the reactant and the product state proceeds along the natural path of the studied chemical reaction. We provide examples of free energy calculations for the fragmentation of the charged clusters and the swapping reaction of oligomer formation in proteins that follow a tentative reaction mechanism. 相似文献
66.
OBJECTIVE: To assess the utility of indigenously prepared cell blocks (CBs) as an adjunct to a conventional smear test in providing a reliable diagnosis of clinically suspicious cervical cancer in resource-limited settings. METHODS: Eighty-six clinically suspicious cervical cancer cases underwent a conventional smear test, CB preparation from residual cellular samples and biopsies at the same sitting. Correlations were performed between these modalities in order to derive the sensitivity and specificity of the CB technique to diagnose cervical cancer. OBSERVATION & RESULTS: Out of 86 clinically suspicious cervical cancers, 72 (83.7%), 70 (81.4%) and 67 (77.9%) cases were diagnosed as malignant on tissue biopsies, CBs and smears respectively. CB-biopsy agreement in the diagnosis of malignancy was feasible in 87.5% of the cases while CB-Pap smear agreement was feasible in 92.5% of the cases. Sensitivity and specificity of CB preparation to diagnose malignancy was 92.5% and 100%, respectively, when the smear was taken as the reference test (excluding the unsatisfactory smears). When biopsy was taken as the gold standard, the sensitivity and specificity of CBs were 87.5% and 100% respectively (excluding the unsatisfactory biopsies). In 8/19 cases where the smear diagnoses were either unsatisfactory or atypical squamous cells/atypical glandular cells, CBs picked up malignant lesions. CONCLUSION: CBs prepared from the residual cellular sample of conventional cervical scrapes augment the sensitivity of the smear test. When used as an adjunct to the smear, CBs aid in providing a reliable diagnosis of cervical cancer in the majority of the clinically suspected cases and thus the biopsy load can be reduced significantly in resource-poor settings. 相似文献
67.
68.
为了合理评价化合物的促神经再生活性.用溴化四唑蓝(MTT)、分化计数、图象处理等方法,借助FK506、GPI1046阳性化合物,建立了一个基于PC12细胞存活和分化的化合物筛选系统.结果表明,无论在细胞存活实验还是在分化实验中,FK506、GPI1046都可以明显增强神经生长因子(NGF)的效应,即有促神经再生的作用.也就是说,这一系统将有助于从组合化学方法合成的化合物文库中,筛选出具有促神经再生活性的化合物. 相似文献
69.
Two new C-15 enolic acyl phragmalin-type limonoid orthoesters (1-2) which possessed a C-15-propionyl phragmalin skeleton and two new mexicanolide-type limonoids (3-4) were isolated from the ethanol extract of seeds of Chukrasia tabularis A. Juss. Their structures were established on the basis of spectroscopic analyses and electronic circular dichroism (ECD) exciton chirality method. Additionally, all of the compounds were screened against three human tumor cell lines MCF-7, SMMC-7721, and U2OS. 相似文献
70.
Drewitz M Helbling M Fried N Bieri M Moritz W Lichtenberg J Kelm JM 《Biotechnology journal》2011,6(12):1488-1496
Although the relevance of three-dimensional (3-D) culture has been recognized for years and exploited at an academic level, its translation to industrial applications has been slow. The development of reliable high-throughput technologies is clearly a prerequisite for the industrial implementation of 3-D models. In this study the robustness of spherical microtissue production and drug testing in a 96-well hanging-drop multiwell plate format was assessed on a standard 96-well channel robotic platform. Microtissue models derived from six different cell lines were produced and characterized according to their growth profile and morphology displaying high-density tissue-like reformation and growth over at least 15 days. The colon cancer cell line HCT116 was chosen as a model to assess microtissue-based assay reproducibility. Within three individual production batches the size variations of the produced microtissues were below 5%. Reliability of the microtissue-based assay was tested using two reference compounds, staurosporine and chlorambucil. In four independent drug testings the calculated IC(50) values were benchmarked against 2-D multiwell testings displaying similar consistency. The technology presented here for the automated production of a variety of microtissues for efficacy testing in a standard 96-well format will aid the implementation of more organotypic models at an early time point in the drug discovery process. 相似文献