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131.
A dual-step fluorescence resonance energy transfer-based quenching assay for screening of caspase-3 inhibitors 总被引:1,自引:0,他引:1
The control of cell death is an intricate process involving a multitude of intracellular modulators. Among these molecules, the caspases have a central role and have become an interesting group of enzymes in the current pharmaceutical industry. We have developed a novel dual-step fluorescence energy transfer-based separation-free assay method for the primary screening of caspase-3 inhibitors in vitro. This method relies on fluorescent europium(III)-chelate-doped nanoparticle donors coated with streptavidin in conjunction with a dual-labeled (N-terminal Alexa Fluor 680 fluorescent acceptor and C-terminal BlackBerry Quencher 650) caspase-3-specific peptide substrate modified with a biotinyl moiety. In the assay, the nanoparticle donor excites the fluorescent acceptor, whose emission is monitored with time-resolved measurements. The intensity of the acceptor reflects the activity of the enzyme because the intensity is controlled by the proximity of the quencher. Owing to the dual-step fluorescence resonance energy transfer, this method enables a sensitized fluorescence signal directly proportional to the extent of enzymatic activity with relatively background fluorescence-free measurements in the event of complete enzyme inhibition. The generic nanoparticle donors further promote versatility and cost-efficiency of the method. The performance evaluated as the inhibitor (Z-DEVD-FMK) dose-response curve (IC(50) value of approximately 12 nM) was in good agreement with that of the recent methods found in literature. This assay serves as a model application proving the feasibility of the europium-chelate-doped nanoparticle labels in a homogeneous assay for proteolytic activity. 相似文献
132.
von Rad U Klein I Dobrev PI Kottova J Zazimalova E Fekete A Hartmann A Schmitt-Kopplin P Durner J 《Planta》2008,229(1):73-85
133.
Cells in living organisms are regulated by chemical and physical stimuli from their environment. Often, ligands interact with
membrane receptors to trigger responses and Sargent and Schwyzer conceived a model to describe this process, “membrane catalysis”.
There is a notion that the physical organization of membranes can control the response of cells by speeding up reactions.
We revisit the “membrane catalysis” model in the light of recent technical, methodological and theoretical advances and how
they can be exploited to highlight the details of membrane mediated ligand–receptor interactions. We examine the possible
effects that ligand concentration causes in the membrane catalysis and focus our attention in techniques used to determine
the partition constant. The hypothetical diffusional advantage associated with membrane catalysis is discussed and the applicability
of existing models is assessed. The role of in-depth location and orientation of ligands is explored emphasizing the contribution
of new analysis methods and spectroscopic techniques. Results suggest that membranes can optimize the interaction between
ligands and receptors through several different effects but the relative contribution of each must be carefully investigated.
We certainly hope that the conjugation of the methodological and technical advances here reported will revive the interest
in the membrane catalysis model. 相似文献
134.
135.
摘要:细菌群体感应(Quorum sensing, QS)被视为对抗细菌感染与解决细菌耐药性问题的新靶点。以AHLs为信号分子的LuxR/I型群体感应系统广泛存在于革兰氏阴性菌包括多种临床致病菌中,因此寻找LuxR/I型群体感应抑制剂(Quorum sensing inhibitors, QSIs)是研发抗革兰氏阴性致病菌药物的重要途径。迄今为止,已知的LuxR/I型小分子QSIs来源包括化学合成、天然产物与已知药物库的化合物,大分子则包括群体感应淬灭酶与群体感应淬灭抗体。本文总结了近年来LuxR/I型QSIs研究进展,为新型抗菌药物研发提供理论依据。 相似文献
136.
Natural polysaccharides, derived from biomass feedstocks, marine resources, and microorganisms, have been attracting considerable attention as benign and environmentally friendly substitutes for synthetic polymeric products. Besides many other applications, these biopolymers are rapidly emerging as viable alternatives to harmful synthetic flocculating agents for the removal of contaminants from water and wastewater. In recent years, a great deal of effort has been devoted to improve the production and performance of polysaccharide bio-based flocculants. In this review, current trends in preparation and chemical modification of polysaccharide bio-based flocculants and their flocculation performance are discussed. Aspects including mechanisms of flocculation, biosynthesis, classification, purification and characterization, chemical modification, the effect of physicochemical factors on flocculating activity, and recent applications of polysaccharide bio-based flocculants are summarized and presented. 相似文献
137.
【背景】抗生素的无序使用加剧了耐药性金黄色葡萄球菌超级菌株的出现,由其引发的感染已成为最难解决的感染性疾患。在生物体系外构建AgrA/C双组分系统的跨膜信号转导过程,对解决金黄色葡萄球菌的耐药性问题和发现新型抗菌药物具有重要的研究意义。【目的】人工模拟构建金黄色葡萄球菌AgrA/C双组分信号转导模型,为生物体外研究金黄色葡萄球菌双组分信号转导的机制及以其为靶点的药物筛选提供新途径。【方法】在大肠杆菌宿主细胞中大量表达AgrA和Agr C蛋白,利用亲和层析和分子筛凝胶层析对其进行分离纯化,利用非放射性凝胶阻滞实验(EMSA)检测AgrA蛋白活性,并检测Agr C激酶活性;进而利用脂质体介导法在体外组装AgrA/C双组分信号转导模型,应用EMSA方法进行评价。【结果】分离纯化得到AgrA和Agr C蛋白,二者纯度均达到90%以上,均具有活性。在生物体系外构建了金黄色葡萄球菌AgrA/C双组分信号转导模型,该系统可增强AgrA对DNA的延滞作用,具有信号传递功能。【结论】初步构建AgrA/C双组分信号转导模型,该模型具有信号传递能力,有望作为针对金黄色葡萄球菌开发新型抗菌药物的筛选平台。 相似文献
138.
Regulation of antimicrobial peptide production by autoinducer-mediated quorum sensing in lactic acid bacteria 总被引:2,自引:0,他引:2
Quadri LE 《Antonie van Leeuwenhoek》2002,82(1-4):133-145
Several lactic acid bacteria produce peptides with antimicrobial activity. During the last few years, cell–cell communication has emerged as the key regulatory mechanism that controls the production of many of these antimicrobial peptides via a regulatory strategy denominated quorum sensing. Quorum sensing allows population-wide synchronised production of antimicrobial peptides as a function of cell density. The cell–cell communication phenomenon required for sensing of the cell density is mediated by secreted signalling molecules. These molecular messengers accumulate in the environment as the cell density increases and activate signal transduction cascades that result in the production of antimicrobial peptides by the stimulated bacterial cell. 相似文献
139.
单增李斯特菌生物膜及其形成机制的研究进展 总被引:1,自引:0,他引:1
单增李斯特菌(Lm)是重要的人兽共患食源性病原菌。Lm生物膜与其致病性和耐药性密切相关。影响Lm生物膜形成的关键因子有鞭毛糖蛋白、胞外基质和群体感应系统等。鞭毛糖蛋白能促进菌体聚集,从而直接影响生物膜的形成。胞外DNA参与Lm粘附和生物膜早期的形成,并与胞外多糖和胞外结合蛋白一起构成生物膜胞外基质。Lm的Agr群体感应系统正调控生物膜形成,是一种集合毒力因子、耐药因子和生物膜的整体水平调控网络体系。 相似文献
140.
《Bioorganic & medicinal chemistry letters》2020,30(17):127398
In this study we developed a novel diagnostic tool for the detection of miRNA21, based on the fluorescent nucleotide morpholine naphthalimide deoxyuridine (dUrkTP). We incorporated dUrkTP into DNA through primer extension to obtain rkDNA displaying high fluorescence. We then used lambda exonuclease, a specific nuclease for 3́-monophosphate–containing DNA, to separate rkDNA from its complementary sequence. The fluorescence of the free rkDNA was quenched dramatically upon interacting with graphene oxide (GO). Our rkDNA–GO fluorescence probing system exhibited high sensitivity and selectivity for the detection of miRNA21. This inexpensive probing system, employing simple primer extension and exonuclease degradation, required only 30 min to detect its target miRNA. This strategy appears suitable for the detection of diverse types of miRNA. 相似文献