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231.
Mitochondrial Remodeling in Endothelial Cells under Cyclic Stretch is Independent of Drp1 Activation
Megumi Baba Aya Shinmura Shigeru Tada Taku Amo Akira Tsukamoto 《Molecular & cellular biomechanics : MCB》2019,16(1):1-12
Mitochondria in endothelial cells remodel morphologically when supraphysiological cyclic stretch is exerted on the cells. During remodeling, mitochondria become shorter, but how they do so remains elusive. Drp1 is a regulator of mitochondrial morphologies. It shortens mitochondria by shifting the balance from mitochondrial fusion to fission. In this study, we hypothesized that Drp1 activation is involved in mitochondrial remodeling under supraphysiological cyclic stretch. To verify the involvement of Drp1, its activation was first quantified with Western blotting, but Drp1 was not significantly activated in endothelial cells under supraphysiological cyclic stretch. Next, Drp1 activation was inhibited with Mdivi-1, but this did not inhibit mitochondrial remodeling. Intracellular Ca2+ increase activates Drp1 through calcineurin. First, we inhibited the intracellular Ca2+ increase with Gd3+ and thapsigargin, but this did not inhibit mitochondrial remodeling. Next, we inhibited calcineurin with cyclosporin A, but this also did not inhibit mitochondrial remodeling. These results indicate that mitochondrial remodeling under supraphysiological cyclic stretch is independent of Drp1 activation. In endothelial cells under supraphysiological cyclic stretch, reactive oxygen species (ROS) are generated. Mitochondrial morphologies are remodeled by ROS generation. When ROS was eliminated with N-acetyl-L-cysteine, mitochondrial remodeling was inhibited. Furthermore, when the polymerization of the actin cytoskeleton was inhibited with cytochalasin D, mitochondrial remodeling was also inhibited. These results suggest that ROS and actin cytoskeleton are rather involved in mitochondrial remodeling. In conclusion, the present results suggest that mitochondrial remodeling in endothelial cells under supraphysiological cyclic stretch is induced by ROS in association with actin cytoskeleton rather than through Drp1 activation. 相似文献
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233.
Pulsatile tinnitus (PT), characterized as pulse-synchronous, is generally objective. Sigmoid sinus (SS) venous sound is widely suggested to be a possible sound source of PT. The dehiscence and thinness of SS cortical plate (CP) was commonly reported as PT pathology in previous studies, but lack quantitative or biomechanical analysis. In this study, it was aimed to quantify the relationship between venous sound and CP dehiscence/thinness using in vitro experiment. The in vitro models of SS and CP were established based on 3D-printing, with the developed pulsatile venous flow in the SS model. The generated sound signal and the vibration response at the dehiscent/thinned area were analyzed. The sound signal generated in the normal-sized dehiscence model was pulse-synchronous within 100-–400 Hz, which had similar acoustic characteristics as the clinical PT sounds. It was concluded that the pulsatile venous sound is produced at TS-SS junction in case of CP dehiscence. The CP, even a thinned one can effectively diminish the venous sound and sound-generating pulsatile vibration at TS-SS junction. The CP dehiscence would induce pulse-synchronous and high pressure venous sound, as well as pulse-synchronous vibration above 20 Hz, regardless of the dehiscence size. On the contrary, the CP thinness would not induce obvious venous sound or pulsatile vibration above 20 Hz. 相似文献
234.
Physical force has emerged as a key regulator of tissue homeostasis, and plays an important role in embryogenesis, tissue regeneration, and disease progression. Currently, the details of protein interactions under elevated physical stress are largely missing, therefore, preventing the fundamental, molecular understanding of mechano-transduction. This is in part due to the difficulty isolating large quantities of cell lysates exposed to force-bearing conditions for biochemical analysis. We designed a simple, easy-to-fabricate, large-scale cell stretch device for the analysis of force-sensitive cell responses. Using proximal biotinylation (BioID) analysis or phospho-specific antibodies, we detected force-sensitive biochemical changes in cells exposed to prolonged cyclic substrate stretch. For example, using promiscuous biotin ligase BirA* tagged α-catenin, the biotinylation of myosin IIA increased with stretch, suggesting the close proximity of myosin IIA to α-catenin under a force bearing condition. Furthermore, using phospho-specific antibodies, Akt phosphorylation was reduced upon stretch while Src phosphorylation was unchanged. Interestingly, phosphorylation of GSK3β, a downstream effector of Akt pathway, was also reduced with stretch, while the phosphorylation of other Akt effectors was unchanged. These data suggest that the Akt-GSK3β pathway is force-sensitive. This simple cell stretch device enables biochemical analysis of force-sensitive responses and has potential to uncover molecules underlying mechano-transduction. 相似文献
235.
It is well recognized that pathologically increased mechanical stretch plays a critical role in vascular remodeling during hypertension. However, how the stretch modulates the functions of ion channels of vascular smooth muscle cells (VSMCs) remains to be elucidated. Here, we demonstrated the effects of mechanical stretch on the activity of large conductance calcium, voltage-activated potassium (BK) and L-type Ca2+ channels. In comparison with 5% stretch (physiological), 15% stretch (pathological) upregulated the current density of L-type Ca2+ and BK channels as well as the frequency and amplitude of calcium oscillation in VSMCs. 15% stretch also increased the open probability and mean open time of the BK channel compared with 5% stretch. BK and L-type Ca2+ channels participated in the mechanical stretch-modulated calcium oscillation. Our results suggested that during hypertension, pathological stretch altered the activity of BK and L-type Ca2+ channels and manipulated the calcium oscillation of VSMCs. 相似文献