全文获取类型
收费全文 | 2112篇 |
免费 | 168篇 |
国内免费 | 48篇 |
专业分类
2328篇 |
出版年
2023年 | 27篇 |
2022年 | 51篇 |
2021年 | 52篇 |
2020年 | 43篇 |
2019年 | 66篇 |
2018年 | 39篇 |
2017年 | 48篇 |
2016年 | 52篇 |
2015年 | 60篇 |
2014年 | 80篇 |
2013年 | 116篇 |
2012年 | 54篇 |
2011年 | 74篇 |
2010年 | 44篇 |
2009年 | 80篇 |
2008年 | 87篇 |
2007年 | 72篇 |
2006年 | 76篇 |
2005年 | 60篇 |
2004年 | 61篇 |
2003年 | 51篇 |
2002年 | 59篇 |
2001年 | 57篇 |
2000年 | 50篇 |
1999年 | 40篇 |
1998年 | 71篇 |
1997年 | 43篇 |
1996年 | 47篇 |
1995年 | 46篇 |
1994年 | 53篇 |
1993年 | 46篇 |
1992年 | 52篇 |
1991年 | 44篇 |
1990年 | 41篇 |
1989年 | 26篇 |
1988年 | 40篇 |
1987年 | 35篇 |
1986年 | 31篇 |
1985年 | 55篇 |
1984年 | 25篇 |
1983年 | 30篇 |
1982年 | 40篇 |
1981年 | 21篇 |
1980年 | 17篇 |
1979年 | 8篇 |
1978年 | 11篇 |
1977年 | 8篇 |
1976年 | 6篇 |
1973年 | 8篇 |
1972年 | 9篇 |
排序方式: 共有2328条查询结果,搜索用时 15 毫秒
1.
Neurotoxic Esterase in Human Nervous Tissue 总被引:1,自引:1,他引:0
2.
Rebecca C. Schreiber Stacey A. Vaccariello Kristen Boeshore Annette M. Shadiack Richard E. Zigmond 《Developmental neurobiology》2002,53(1):68-79
Transecting the axons of neurons in the adult superior cervical ganglion (SCG; axotomy) results in the survival of most postganglionic neurons, the influx of circulating monocytes, proliferation of satellite cells, and changes in neuronal gene expression. In contrast, transecting the afferent input to the SCG (decentralization) results in nerve terminal degeneration and elicits a different pattern of gene expression. We examined the effects of decentralization on macrophages in the SCG and compared the results to those previously obtained after axotomy. Monoclonal antibodies were used to identify infiltrating (ED1+) and resident (ED2+) macrophages, as well as macrophages expressing MHC class II molecules (OX6+). Normal ganglia contained ED2+ cells and OX6+ cells, but few infiltrating macrophages. After decentralization, the number of infiltrating ED1+ cells increased in the SCG to a density about twofold greater than that previously seen after axotomy. Both the densities of ED2+ and OX6+ cells were essentially unchanged after decentralization, though a large increase in OX6+ cells occurred after axotomy. Proliferation among the ganglion's total non‐neuronal cell population was examined and found to increase about twofold after decentralization and about fourfold after axotomy. Double‐labeling experiments indicated that some of these proliferating cells were macrophages. After both surgical procedures, the percentage of proliferating ED2+ macrophages increased, while neither procedure altered the proliferation of ED1+ macrophages. Axotomy, though not decentralization, increased the proliferation of OX6+ cells. Future studies must address what role(s) infiltrating and/or resident macrophages play in regions of decentralized and axotomized neurons and, if both are involved, whether they play distinct roles. © 2002 Wiley Periodicals, Inc. J Neurobiol 53: 68–79, 2002 相似文献
3.
Kaeko Tozawa Eiji Arakawa Toshiyuki Chikuma Yoshihiro Oh-hashi Ryuichi Yajima Katsumichi Takeda Hiroshi Shinozaki† Takeshi Kato† 《Journal of neurochemistry》1990,55(3):745-749
Axonal transport of peptidylglycine alpha-amidating monooxygenase (PAM) activity was studied in rat sciatic nerves from 12 to 120 h after double ligations. The anterograde axonal transport increased and reached a plateau between 48 and 72 h and then decreased. The flow rate was 100 mm/day, and the molecular mass of the active entity was 70 kDa, which was determined by gel filtration. In contrast, there was no evidence for significant retrograde axonal transport. Anterograde axonal transport of immunoreactive cholecystokinin, a carboxy-terminal-amidated putative neuropeptide, was also found. These results suggest that PAM is transported by a rapid axonal flow and may play a role as a processing enzyme during transport or in the terminals of rat sciatic nerves. 相似文献
4.
5.
Ellen J. Lehning Renu Doshi Norman Isaksson Peter K. Stys Richard M. LoPachin Jr. 《Journal of neurochemistry》1996,66(2):493-500
Abstract: To investigate the route of axonal Ca2+ entry during anoxia, electron probe x-ray microanalysis was used to measure elemental composition of anoxic tibial nerve myelinated axons after in vitro experimental procedures that modify transaxolemmal Na+ and Ca2+ movements. Perfusion of nerve segments with zero-Na+/Li+-substituted medium and Na+ channel blockade by tetrodotoxin (1 µM) prevented anoxia-induced increases in Na and Ca concentrations of axoplasm and mitochondria. Incubation with a zero-Ca2+/EGTA perfusate impeded axonal and mitochondrial Ca accumulation during anoxia but did not affect characteristic Na and K responses. Inhibition of Na+-Ca2+ exchange with bepridil (50 µM) reduced significantly the Ca content of anoxic axons although mitochondrial Ca remained at anoxic levels. Nifedipine (10 µM), an L-type Ca2+ channel blocker, did not alter anoxia-induced changes in axonal Na, Ca, and K. Exposure of normoxic control nerves to tetrodotoxin, bepridil, or nifedipine did not affect axonal elemental composition, whereas both zero-Ca2+ and zero-Na+ solutions altered normal elemental content characteristically and significantly. The findings of this study suggest that during anoxia, Na+ enters axons via voltage-gated Na+ channels and that subsequent increases in axoplasmic Na+ are coupled functionally to extraaxonal Ca2+ import. Intracellular Na+-dependent, extraaxonal Ca2+ entry is consistent with reverse operation of the axolemmal Na+-Ca2+ exchanger, and we suggest that this mode of Ca2+ influx plays a general role in peripheral nerve axon injury. 相似文献
6.
7.
Susumu Kimura 《Microbiology and immunology》1996,40(9):645-650
Reactivation of herpes simplex virus type 1 (HSV-1) occurred rapidly in cells of latently infected adult mouse trigeminal ganglia which were cultured in serum-free medium in the presence of sufficient nerve growth factor (NGF). However, HSV-1 reactivation was delayed significantly in ganglionic cultures in the absence of exogenous NGF or in cultures treated with 2-aminopurine in the presence of NGF. The delayed viral reactivation in ganglionic cultures without NGF was accelerated by treatment with phorbol myristate acetate or dibutyryl cyclic AMP. Culture conditions which affected HSV-1 reactivation did not affect replication of HSV-1 in normal ganglionic cultures. 相似文献
8.
9.
10.
Developmental Changes in Glycolipid Synthesizing Enzymes in the Brain of a Myelin-Deficient Mutant Wistar Rat 总被引:2,自引:1,他引:1
Changes in the activities of UDP-galactose:ceramide galactosyltransferase (CGalT, EC 2.4.1.45), UDP-glucose:ceramide glucosyltransferase (CGlcT, EC 2.4.1.80) and 3'-phosphoadenosine-5'-phosphosulfate (PAPS): galactosylceramide 3'-sulfotransferase (EC 2.8.2.11) over the myelinating period between 12 and 25 days were studied in the brains of control and myelin-deficient rats. Although the activity of galactosyltransferase with ceramides containing hydroxy fatty acids quadrupled in normal male littermates between 14 and 20 days, hardly any increase was observed in the mutant and the activity was less than 10% of control above 20 days of age. With normal fatty acid containing ceramides as acceptors, the activity decreased from 83% of the control at 12 days to approximately 30% after 20 days. Sulfotransferase activity also did not show the normal increase during the 3rd week of life and declined from 60% to 22%. Glucosyltransferase and lysosomal hydrolases in brain and ceramide galactosyltransferase in sciatic nerves appeared to be normal. These results suggest close similarities to the jimpy mutant mouse in which myelin deficiency is also inherited as an x-linked recessive trait. 相似文献