全文获取类型
收费全文 | 218篇 |
免费 | 24篇 |
国内免费 | 45篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 8篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 11篇 |
2014年 | 3篇 |
2013年 | 16篇 |
2012年 | 6篇 |
2011年 | 12篇 |
2010年 | 3篇 |
2009年 | 10篇 |
2008年 | 10篇 |
2007年 | 9篇 |
2006年 | 15篇 |
2005年 | 11篇 |
2004年 | 6篇 |
2003年 | 7篇 |
2002年 | 13篇 |
2001年 | 13篇 |
2000年 | 11篇 |
1999年 | 9篇 |
1998年 | 9篇 |
1997年 | 3篇 |
1996年 | 8篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1976年 | 1篇 |
排序方式: 共有287条查询结果,搜索用时 31 毫秒
71.
S. HASAN 《The Annals of applied biology》1991,118(1):19-25
Onion weed, Asphodelus fistulosus L., (Liliaceae) a weed of Mediterranean and Middle Eastern origin is widespread in southern Australia where it invades pastures making them unsuitable for grazing. A program of research is underway to discover natural enemies of this plant and to study their possible role in the biological control of onion weed. A rust fungus Puccinia barbeyi (Roum.) Magnus has been found to severely attack A. fistulosus . Observations on the biology of the rust confirmed that it is monoecious and microcyclic and multiplied essentially by aecial and telial stages, although occasionally urediniospores also appeared among teliospores. Several members of the Liliaceae exposed to the aeciospores of the rust remained unattacked indicating that it is most probably specific to Asphodelus spp. and thus its potential for the biological control of A. fistulosus in Australia should be studied further. 相似文献
72.
A fragment of the mitochondrial cytochrome b (cyt b) gene of 13 agronomically important plant pathogenic Basidiomycetes was sequenced, including several Puccinia spp., Uromyces appendiculatus, Phakopsora pachyrhizi, Hemileia vastatrix and Rhizoctonia solani. The deduced amino acid sequences (residues 142–266) were used to study the relatedness of these pathogens as compared to other species of the Basidiomycetes, Ascomycetes and Oomycetes. The relatedness was also studied at nuclear level using the Internal Transcribed Spacers (ITS) in the ribosomal DNA. Phylogenic trees were constructed with the maximum parsimony (MP) and the neighbour‐joining (NJ) methods. On the basis of both cytochrome b and ITS sequences, the Puccinia species pathogenic to graminaceous crop plants, such as Puccinia recondita f. sp. tritici, Puccinia graminis f. sp. tritici, Puccinia striiformis f. sp. tritici, Puccinia coronata f. sp. avenae, Puccinia hordei, P. recondita f. sp. secalis and Puccinia sorghi, together with Puccinia horiana from Chrysanthemum, were very closely related to each other, whereas Puccinia arachidis (from peanut) was closely related to U. appendiculatus (from beans) but more distant from the other Puccinia species. Both rusts on soybean (P. pachyrhizi) and coffee (H. vastatrix) were outside the Puccinia cluster. All rusts were separated from other Basidiomycetes such as R. solani and the strobilurin producing species Strobilurus tenacellus and Mycena galopoda. Our results demonstrate that the amino acid sequence of the mitochondrial cytochrome b is a valid tool to study phylogenic relatedness among plant pathogenic Basidiomycetes and supports taxonomic grouping based on morphological structures and host specificity. Because of their high variability, ITS sequences were able to discriminate Puccinia species, which were identical on the basis of the cytochrome b amino acid sequence. Thus, ITS sequences could better show differences among species or within a species, whereas cytochrome b is more suitable than ITS for phyologenic inference at family or genus level. In addition, the sequence data obtained during this study represent essential information for easy isolation of the cyt b gene and detection of point mutations conferring resistance to Qo Inhibitor fungicides that eventually may evolve. 相似文献
73.
K. R. Gunaratna R. Balasubramanian 《World journal of microbiology & biotechnology》1994,10(3):342-345
An extracellular chitinase of Acremonium obclavatum was partially purified. It had an M
r of 45 kDa on SDS-PAGE, and was optimally active at pH 3 to 4 and 50°C. Hg and Mn (10 mm) inhibited activity. The chitinase hydrolysed colloidal chitin more rapidly than crude chitin or isolated A. obclavatum cell walls. The partially-purified enzyme inhibited uredospore germination and germ-tube growth of Puccinia arachidis.The authors are with the Centre for Advanced Study in Botany, University of Madras, Guindy campus, Madras 600 025, India 相似文献
74.
Hyphal confrontation between two haploid cultures originating from single basidiospores was used to determine the mating type
ofPuccinia coronata var.coronata. Pairs of 15 single-basidiospore cultures were placed approximately 1 mm apart on the medium in all possible combinations.
Hyphae of the pairs of colonies came into contact with each other in all combinations approximately two weeks after confrontations.
When the nuclear number of hyphal cells in a contact zone was investigated one month after confrontation, monokaryotic hyphae
were observed in selfing combination. On the other hand, dikaryotic hyphae were observed in 90.5% of crossing combinations
between different cultures. Two isolates are considered compatible if dikaryotic hyphae are present in the contact zone but
incompatible if they are absent. The mating type of the fungus was found to be characterized by multiple-allelomorphic tetrapolar
incompatibility controlled by the “A” and “B” incompatible factors.
Contribution No. 116, Laboratories of Plant Pathology and Mycology, Institute of Agriculture and Forestry, University of Tsukuba 相似文献
75.
X. Qi G. Jiang W. Chen R. E. Niks P. Stam P. Lindhout 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(5):877-884
By using a high-density AFLP marker linkage map, six QTLs for partial resistance to barley leaf rust (Puccinia hordei) isolate 1.2.1. have been identified in the RIL offspring of a cross between the partially resistant cultivar ’Vada’ and
the susceptible line L94. Three QTLs were effective at the seedling stage, and five QTLs were effective at the adult plant
stage. To study possible isolate specificity of the resistance, seedlings and adult plants of the 103 RILs from the cross
L94×’Vada’ were also inoculated with another leaf rust isolate, isolate 24. In addition to the two QTLs that were effective
against isolate 1.2.1. at the seedling stage, an additional QTL for seedling resistance to isolate 24 was identified on the
long arm of chromosome 7. Of the eight detected QTLs effective at the adult plant stage, three were effective in both isolates
and five were effective in only one of the two isolates. Only one QTL had a substantial effect at both the seedling and the
adult plant stages. The expression of the other QTLs was developmental-stage specific. The isolate specificity of the QTLs
supports the hypothesis of Parlevliet and Zadoks (1977) that partial resistance may be based on a minor-gene-for-minor-gene
interaction.
Received: 16 February 1999 / Accepted: 20 February 1999 相似文献
76.
In wheat (Triticum aestivum cv. Soissons) plants grown under three different fertilisation treatments, we quantified the effect of leaf rust (Puccinia triticina) on flag leaf photosynthesis during the whole sporulation period. Bastiaans' model: Y = (1 - x)beta was used to characterize the relationship between relative leaf photosynthesis (Y) and disease severity (x). The evolution of the different types of symptoms induced by the pathogen (sporulating, chlorotic and necrosed tissues) was evaluated using image analysis. The beta-values varied from 2 to 11, 1.4-2, and 0.8-1 during the sporulation period, when considering the proportion of sporulating, sporulating + necrotic, and total diseased area, respectively. Leaf nitrogen (N) content did not change the effect of the disease on host photosynthesis. We concluded that leaf rust has no global effect on the photosynthesis of the symptomless parts of the leaves and that the large range in the quantification of leaf rust effect on the host, which is found in the literature, can be accounted for by considering the different symptom types. We discuss how our results could improve disease assessments and damage prediction in a wheat crop. 相似文献
77.
K. Ramdoyal S. Sullivan L. C. Y. Lim Shin Chong G. H. Badaloo S. Saumtally R. Domaingue 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):557-563
The inheritance of rust resistance was studied in sugar cane seedling populations using a factorial mating design over 1 summer
and 2 winter seasons. Frequency distributions for rust infection pooled over 2 winter seasons for resistant x resistant parents
were highly skewed with the majority of progenies grouped towards the resistant classes, whereas crosses between susceptible
x highly susceptible parents tended to be skewed with the majority of progenies grouped towards the susceptible ones. Both
categories of crosses produced transgressive segregants at either extremes. Distribution of infection within progeny of the
selfed resistant parent ’R 570’ and distribution in the majority of crosses tended to support the hypothesis of a major gene
with a dominant effect for resistance. However, the action of other minor genes acting in a quantitative way is also suggested.
The female (F) and male (M) variance components were very important, and F×M interaction indicated the existence of non-additive
genetic effects. F×S, M×S and F×M×S interaction mean squares were generally low or insignificant. Broad-sense heritability
for the individual season ratings and for the combined ratings was high (0.75–0.90), whereas narrow-sense heritability was
generally moderate (0.40–0.52) with the additive genetic effects accounting for 44–68% of the total genetic variation. The
implications of these findings in the breeding for rust resistance in the local programme are discussed.
Received: 2 June 1999 / Accepted: 22 June 1999 相似文献
78.
A. Börner M. S. Röder O. Unger A. Meinel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(7):1095-1099
A major gene determining non-specific adult-plant disease resistance against stripe rust (Puccinia striiformis) designated Yrns-B1 was mapped by using a cross between ’Lgst.79–74’ (resistant) and ’Winzi’ (susceptible). Analyzing F3 lines of two consecutive experimental years contrary modes of inheritance were observed due to the intermediate character
of the gene and the difference in the disease pressure during the seasons. Using the disease scoring data of both experimental
years independently two maps were constructed detecting Yrns-B1 20.5 and 21.7 cM, respectively, proximal to the wheat microsatellite (WMS) marker Xgwm493 on the short arm of chromosome 3BS. The genetic relationships to other major genes or to quantitative trait loci controlling
adult plant disease resistance against rusts in wheat are discussed.
Received: 27 May 1999 / Accepted: 28 September 1999 相似文献
79.
The biology of islands differs from that of large land masses in having less complex ecosystems. Introduced exotic weeds are often a major threat to fragile island ecosystems because of their expansion into habitats previously occupied by endemic species. San Clemente Island, 120 km off the California coastline, is an example of this process in which numerous exotic weed species have been introduced and some are endangering the native flora. Crown rust of oats caused by Puccinia coronata f.sp. avenae was investigated as a potential biocontrol agent against two wild oat species, Avena barbata and Avena fatua, introduced on San Clemente Island. Epidemiology and virulence of this rust were studied. The island was surveyed from 1995 to 1998 for occurrence of P. coronata on wild oats. Wild oats were found sprouting in the northern part of the island shortly after autumn rainfall and subsequently covered the main grasslands of the island. The rust also appeared first on the northern part of the island and progressively spread south. However, disease severities in the south were considerably lower than those in the north. Diverse virulence types, although related to Californian and Mexican forms, were detected among the isolates. The potential use of P. coronata as an augmentative biocontrol agent for wild oat species on San Clemente Island is discussed. 相似文献
80.
感染叶锈菌的小麦细胞间隙液中激发子的定性及初步分离 总被引:6,自引:1,他引:5
感染叶锈菌后小麦叶片细胞间隙液(1wF)中有激发子存在,它(们)能诱导寄主PAL、PO活性的增强及细胞过敏性坏死的产生。这种有诱导活性的物质分别用NaIO4和蛋白酶处理证明含有糖基和蛋白质成分,可能是糖蛋白。IWF经凝胶过滤分离后,各部分诱导不同的防卫反应的活性强度不等。因此IWF中可能含有几种不同成分的激发子,或是同种成分而聚合度不同的激发子。 相似文献