Increased levels of serum galectin-3 in patients with primary Sjögren’s syndrome: Associated with interstitial lung disease

ObjectivesTo explore the potential values of serum galectin-3 (Gal-3) levels in diagnosis of interstitial lung disease (ILD) for patients with primary Sjögren’s syndrome (pSS).MethodsThe concentrations of serum Gal-3 and interleukin (IL)-17 were measured by enzymelinked immunosorbent assay (ELISA) in 87 patients with pSS and 30 healthy controls (HC). The levels of plasma C-reactive protein (CRP), rheumatoid factor (RF), immunoglobulin (Ig)G, complement (C3), albumin (ALB) and Fibrinogen (FIB) and erythrocyte sedimentation rate (ESR) were measured. ILD was identified on high-resolution computed tomography.ResultsThe levels of serum Gal-3 and IL-17 were significantly higher in pSS patients than in HC. Stratification analyses indicated significantly higher levels of Gal-3 in pSS patients with ILD and in those with positive ANCA. In comparison with that of pSS patients without ILD, significantly higher levels of ESR, CRP, FIB, IgG, C3 and lower ALB were detected in pSS patients with ILD. The levels of galectin-3 were correlated positively with the values of CRP, FIB, IgG or IL-17 in patients with pSS.ConclusionsThese findings suggest that higher levels of serum galectin-3 may be associated with the development of pSS, particularly with ILD.     

稀土磁疗对开胸术后机体免疫功能及疼痛的影响

目的:研究稀土磁治疗床对食管癌、肺癌手术患者术后机体免疫力及切口疼痛的影响。方法:选食管癌患者37例,肺癌23例,采集手术前及稀土磁治疗床治疗后患者静脉血,检测T细胞亚群CD3 、CD4 、CD8 、CD4 /CD8 比值和NK细胞的比例变化,检查手术切口疼痛的恢复情况。结果:术后给予稀土磁治疗床治疗后与手术前相比较CD3 、CD4 、NK、CD4 /CD8 细胞有显著提高(P<0.05),也高于对照组术后的T细胞亚群水平(P<0.05)。结论:稀土磁治疗床可提高食管癌、肺癌患者术后的机体免疫力,减轻疼痛,有利于患者术后恢复。     

Synthesis and characterization of isopropylamine complexes of lanthanide(II) diiodides: Molecular structure of TmI2(PrNH2)4 and EuI2(PrNH2)4

It was found that the lanthanide diiodides LnI₂ (1) (Ln = Nd, Sm, Eu, Dy, Tm, Yb) are dissolved in isopropylamine (IPA) without redox transformations. Stability of the formed solutions decreases in a row Eu ≈ Yb > Sm > Tm > Dy > Nd. Removing of a solvent in vacuum leaves complexes LnI₂(IPA)_x (2) (Nd, x = 5; Sm, Eu, Dy, Tm, Yb, x = 4) as crystalline colored solids. Stability of 2-Nd,Dy,Tm is higher than that of known THF or DME coordinated salts. Divalent state of metal in the products is confirmed by data of UV-Vis spectroscopy, magnetic measurements and their chemical behavior. Structure of 2-Eu and 2-Tm was established by X-ray diffraction analysis. Oxidation of 2-Nd,Dy in IPA affords amine-amides (PrⁱNH)Ln(IPA)_y (3) (Nd, y = 4; Dy, x = 3). n-Propylamine also dissolves the iodides 1-Sm,Eu,Dy,Tm,Yb but stability of the solutions is significantly lower. 1-Nd vigorously reacts with PrⁿNH₂ even at −30 °C which hampers the formation of the solution.     

The pollination ecology of Pedicularis rex subsp. lipkyana and P. rex subsp. rex (Orobanchaceae) from Sichuan, southwestern China

Pedicularis is one of few genera for which pollination ecology has been studied extensively. Although over half of the species of Pedicularis are found in the mountains of southwestern China, pollination ecology has been studied there on a few species only. The present paper reports pollination ecology of Pedicularis rex subsp. lipskyana and P. rex subsp. rex from Sichuan, southwestern China. The two subspecies are nectariferous, with sugar content 28% in P. rex lipskyana and 22% in P. rex, respectively. The flowers of the two subspecies are adapted to pollination by bumblebee workers. Bumblebees are the primary and effective pollinators although honeybees were also observed pollinating P. rex subsp. lipskyana at one studied population. Usually bumblebees entered the corolla tube from the right side in an upright or a nearly half-inverted position to imbibe nectar located at the base of the corolla tube. The stigma usually contacts the side region of the thorax and abdomen or occasionally directly contacted pollen loads, leading to pollination of flowers. Much less commonly, bumblebees foraged on flowers of P. rex subsp. rex in an inverted position for pollen, which pollinated flowers sternotribically. We suggest that pollination mechanism is closely associated with floral morphology. Some important differences were found from a previous study on P. rex in floral morphology and the primary pollination mechanism.     

Plasticity of fibroblasts demonstrated by tissue-specific and function-related proteome profiling

Background

Fibroblasts are mesenchymal stromal cells which occur in all tissue types. While their main function is related to ECM production and physical support, they are also important players in wound healing, and have further been recognized to be able to modulate inflammatory processes and support tumor growth. Fibroblasts can display distinct phenotypes, depending on their tissue origin, as well as on their functional state.

Results

In order to contribute to the proteomic characterization of fibroblasts, we have isolated primary human fibroblasts from human skin, lung and bone marrow and generated proteome profiles of these cells by LC-MS/MS. Comparative proteome profiling revealed characteristic differences therein, which seemed to be related to the cell’s tissue origin. Furthermore, the cells were treated in vitro with the pro-inflammatory cytokine IL-1beta. While all fibroblasts induced the secretion of Interleukins IL-6 and IL-8 and the chemokine GRO-alpha, other inflammation-related proteins were up-regulated in an apparently tissue-dependent manner. Investigating fibroblasts from tumorous tissues of skin, lung and bone marrow with respect to such inflammation-related proteins revealed hardly any conformity but rather individual and tumor type-related variations. However, apparent up-regulation of IGF-II, PAI-1 and PLOD2 was observed in melanoma-, lung adenocarcinoma- and multiple myeloma-associated fibroblasts, as well as in hepatocellular carcinoma-associated fibroblasts.

Conclusions

Inflammation-related proteome alterations of primary human fibroblasts were determined by the analysis of IL-1beta treated cells. Tumor-associated fibroblasts from different tissue types hardly showed signs of acute inflammation but displayed characteristic functional aberrations potentially related to chronic inflammation. The present data suggest that the state of the tumor microenvironment is relevant for tumor progression and targeted treatment of tumor-associated fibroblasts may support anti-cancer strategies.

Electronic supplementary material

The online version of this article (doi:10.1186/1559-0275-11-41) contains supplementary material, which is available to authorized users.     

miR-224 在原发性肝癌患者血清中的表达及意义

目的：原发性肝癌(primary hepatocellular carcinoma, PHC) 作为常见的恶性程度极高的肿瘤,严重威胁着人类的生命。 miR-224 是近年来发现的一个肿瘤相关miRNA 分子,在肿瘤的发生及发展过程中发挥着重要的作用。本研究通过测定原发性肝 癌患者血清中miR-224 的表达水平,探讨血清miR-224 与原发性肝癌预后的关系。方法：采用实时荧光定量RT-PCR (Real-time RT-PCR)方法,分别检测40 例原发性肝癌患者,20例慢性肝炎患者,20 例慢性肝硬化患者及20 例正常人的血清标本中miR-224 的表达水平。分析血清miR-224 的表达水平与AFP和MMP-9的相关性。结果：原发性肝癌患者血清miR-224 的表达水平明显高 于正常人、慢性肝炎和慢性肝硬化患者(P<0.05)。皮尔森相关分析结果显示原发性肝癌患者血清miR-224 的表达与AFP 和 MMP-9 呈正相关。血清miR-224 低表达组术后复发/转移率显著低于高表达组,术后生存率则高于高表达组(P<0.01)。结论： miR-224 在原发性肝癌患者的血清中呈高表达,其血清表达水平与原发性肝癌的临床预后密切相关。这提示我们,miR-224 可能 成为新的原发性肝癌检测标记物和潜在的原发性肝癌预后分子标志物。     

超声造影对原发性肝癌数字减影血管造影引导下肝动脉化疗栓塞治疗的疗效评估价值

目的:探讨超声造影评估原发性肝癌(HCC)数字减影血管造影(DSA)引导下肝动脉化疗栓塞(TACE)治疗疗效的价值。方法:选择2016年10月至2018年10月我院收治的77例HCC患者,均在DSA引导下行TACE治疗,治疗前后采用超声造影评价疗效。结果:77例HCC患者均顺利完成TACE治疗,治疗后始增强度、增强速率降低(P0.05),始增时间、达峰时间、峰值加速时间增加(P0.05)。治疗后完全缓解(CR)+部分缓解(PR)为有效共46例(有效组),稳定(SD)+进展(PD)为无效共31例(无效组),有效组治疗后始增强度、增强速率低于无效组(P0.05),始增时间、达峰时间、峰值加速时间多于无效组(P0.05)。受试者工作特征曲线(ROC)分析结果显示,始增时间、达峰时间、峰值加速时间、始增强度、增强速率预测HCC患者DSA引导下TACE治疗疗效的曲线下面积(AUC)分别为0.658、0.717、0.64、0.668、0.713。结论:超声造影定量参数在HCC患者DSA引导下TACE治疗前后有着较为明显的变化,其超声参数达峰时间、增强速率对DSA引导下TACE治疗疗效的预测效能较好。     

多原发癌

目的:探讨多原发癌的病因、临床特点及其诊治。方法:回顾性总结分析了一组普通外科多原发癌病人的资料,并结合文献进行了分析。结果:本组77例多原发癌,占同期普通外科住院病人的1.33%(77/5768),半数以上发生于65岁及以上的老年人。胃肠道多原发癌占81.8%。其发生主要与下列因素有关:基因缺陷因素(遗传易感性)、环境因素、治疗所致(如放、化疗)、免疫缺陷、老龄。结论:多原发癌近年呈增加趋势,对于肿瘤病人的诊治,不要忽略了同时性癌的可能;手术仍是多原发癌实体瘤主要而有效的治疗手段。     

腹腔镜联合胆道镜治疗胆总管结石行胆管一期缝合的可行性研究

目的:探讨应用腹腔镜联合胆道镜胆总管切开取石并行一期缝合胆总管的方法治疗胆总管结石病的可行性。方法:采用前瞻性研究方法,将我院2013年6月~2016年6月收治的76例胆总管结石患者随机分成A、B两组,A组给予腹腔镜联合胆道镜胆总管切开取石后行胆管一期缝合;B组给予腹腔镜联合胆道镜胆总管切开取石后留置T管引流。比较分析两组术中、术后及并发症情况。结果:胆总管一期缝合组较留置T管组住院时间更短,住院费用更低,且术后出现的并发症几率更小。结论:腹腔镜联合胆道镜胆总管切开取石后一期缝合胆总管是治疗胆总管结石病的安全可行的方法,既为患者节省了费用又避免留置T管带来的并发症,在合适的适应症下可行此手术方法治疗胆总管结石病。