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21.
Danielle M.P. Oliveira Fabio M. Gomes Danielle B. Carvalho Isabela Ramos Alan B. Carneiro Mario A.C. Silva-Neto Wanderley de Souza Ana P.C.A. Lima Kildare Miranda Ednildo A. Machado 《Journal of insect physiology》2013,59(12):1242-1249
Despite being the main insect pest on soybean crops in the Americas, very few studies have approached the general biology of the lepidopteran Anticarsia gemmatalis and there is a paucity of studies with embryo formation and yolk mobilization in this species. In the present work, we identified an acid phosphatase activity in the eggs of A. gemmatalis (agAP) that we further characterized by means of biochemistry and cell biology experiments. By testing several candidate substrates, this enzyme proved chiefly active with phosphotyrosine; in vitro assays suggested a link between agAP activity and dephosphorylation of egg yolk phosphotyrosine. We also detected strong activity with endogenous and exogenous short chain polyphosphates (PolyP), which are polymers of phosphate residues involved in a number of physiological processes. Both agAP activity and PolyP were shown to initially concentrate in small vesicles clearly distinct from typically larger yolk granules, suggesting subcellular compartmentalization. As PolyP has been implicated in inhibition of yolk proteases, we performed in vitro enzymatic assays with a cysteine protease to test whether it would be inhibited by PolyP. This cysteine protease is prominent in Anticarsia egg homogenates. Accordingly, short chain PolyP was a potent inhibitor of cysteine protease. We thereby suggest that PolyP hydrolysis by agAP is a triggering mechanism of yolk mobilization in A. gemmatalis. 相似文献
22.
Thermodynamic solubility is a decisive physicochemical property in drug development. The Chasing Equilibrium method offers an alternative to the classical procedures to measure the solubility of compounds with acid–base properties. The method is fast and yields accurate results. In this work, the solubility of several compounds including acids and bases was determined through the Chasing Equilibrium approach. A study of experimental conditions in terms of sample weight was performed to measure solubilities. The study shows that only a limited range of weights, depending on the nature and solubility of the compounds, is adequate to obtain reliable results. 相似文献
23.
Masayuki Adachi Masatomo So Kazumasa Sakurai József Kardos Yuji Goto 《The Journal of biological chemistry》2015,290(29):18134-18145
Although amyloid fibrils and amorphous aggregates are two types of aggregates formed by denatured proteins, their relationship currently remains unclear. We used β2-microglobulin (β2m), a protein responsible for dialysis-related amyloidosis, to clarify the mechanism by which proteins form either amyloid fibrils or amorphous aggregates. When ultrasonication was used to accelerate the spontaneous fibrillation of β2m at pH 2.0, the effects observed depended on ultrasonic power; although stronger ultrasonic power effectively accelerated fibrillation, excessively strong ultrasonic power decreased the amount of fibrils formed, as monitored by thioflavin T fluorescence. An analysis of the products formed indicated that excessively strong ultrasonic power generated fibrillar aggregates that retained β-structures but without high efficiency as seeds. On the other hand, when the spontaneous fibrillation of β2m was induced at higher concentrations of NaCl at pH 2.0 with stirring, amorphous aggregates became more dominant than amyloid fibrils. These apparent complexities in fibrillation were explained comprehensively by a competitive mechanism in which supersaturation-limited reactions competed with supersaturation-unlimited reactions. We link the kinetics of protein aggregation and a conformational phase diagram, in which supersaturation played important roles. 相似文献
24.
目的:制备奈妥吡坦/β-环糊精包合物,用以提高奈妥吡坦的水溶性.方法:采用饱和水溶液法,制备奈妥吡坦/β-环糊精包合物;以载药量为指标,考察奈妥吡坦与β-环糊精的质量比(芯壁比)、包合温度、包合时间、搅拌速度的影响.基于单因素试验结果,采用正交设计实验对制备处方和工艺进行优化,得到最优奈妥吡坦/β-环糊精包合物,并对其... 相似文献
25.
26.
《Bioorganic & medicinal chemistry letters》2020,30(11):127159
Indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO) are promising drug development targets due to their implications in pathologies such as cancer and neurodegenerative diseases. The search for IDO1 inhibitor has been intensely pursued but there is a paucity of potent TDO and IDO1/TDO dual inhibitors. Natural product tryptanthrin has been confirmed to bear IDO1 and/or TDO inhibitory activities. Herein, twelve novel tryptanthrin derivatives were synthesized and evaluated for the IDO1 and TDO inhibitory potency. All of the compounds were found to be IDO1/TDO dual inhibitors, in particular, compound 9a and 9b bore IDO1 inhibitory activity similar to that of INCB024360, and compound 5a and 9b had remarkable TDO inhibitory activity superior to that of the well-known TDO inhibitor LM10. This work enriches the collection of IDO1/TDO dual inhibitors and provides chemical molecules for potential development into drugs. 相似文献
27.
Preformulation studies were performed on a hemiglutarate ester prodrug of Δ9-tetrahydrocannabinol (THC-HG), to facilitate the development of stable formulations by hot-melt methods. The various studies
performed included solid-state thermal characterization, pKa, logP, aqueous and pH dependent solubility, pH stability and
effect of moisture, temperature and oxygen on solid-state stability. A hot-melt method was utilized to fabricate THC-HG incorporated
poly (ethylene oxide) (PEO) matrices and the bioadhesive properties, release profiles and post-processing stability of these
matrices were assessed as a function of the polymer molecular weight. The prodrug exhibited a T
g close to 0°C, indicating its amorphous nature. Thermogravimetric analysis revealed a rapid weight loss after 170°C. The prodrug
exhibited a seven-fold higher aqueous solubility as compared to the parent drug (THC). Also, the solubility of the compound
increased with increasing pH, being maximum at pH 8. The prodrug exhibited a v-shaped pH-rate profile, with the degradation
rate minimum between pH 3 and 4. The moisture uptake and drug degradation increased with an increase in relative humidity.
Solid-state stability indicated that the prodrug was stable at −18°C but demonstrated higher degradation at 4°C, 25°C and
40°C (51.6%, 74.5% and 90.1%, respectively) at the end of 3-months. THC-HG was found to be sensitive to the presence of oxygen.
The release of the active from the polymeric matrices decreased, while bioadhesion increased, with an increase in molecular
weight of PEO. 相似文献
28.
Chitosan functional properties 总被引:7,自引:0,他引:7
Chitosan is a partially deacetylated polymer of N-acetyl glucosamine. It is essentially a natural, water-soluble, derivative
of cellulose with unique properties. Chitosan is usually prepared from chitin (2 acetamido-2-deoxy β-1,4-D-glucan) and chitin
has been found in a wide range of natural sources (crustaceans, fungi, insects, annelids, molluscs, coelenterata etc.) However
chitosan is only manufactured from crustaceans (crab and crayfish) primarily because a large amount of the crustacean exoskeleton
is available as a by product of food processing. Squid pens (a waste byproduct of New Zealand squid processing) are a novel,
renewable source of chitin and chitosan. Squid pens are currently regarded as waste and so the raw material is relatively
cheap. This study was intended to assess the functional properties of squid pen chitosan. Chitosan was extracted from squid
pens and assessed for composition, rheology, flocculation, film formation and antimicrobial properties. Crustacean chitosans
were also assessed for comparison. Squid chitosan was colourless, had a low ash content and had significantly improved thickening
and suspending properties. The flocculation capacity of squid chitosan was low in comparison with the crustacean sourced chitosans.
However it should be possible to increase the flocculation capacity of squid pen chitosan by decreasing the degree of acetylation.
Films made with squid chitosan were more elastic than crustacean chitosan with improved functional properties. This high quality
chitosan could prove particularly suitable for medical/analytical applications.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
29.
Cabantous S Pédelacq JD Mark BL Naranjo C Terwilliger TC Waldo GS 《Journal of structural and functional genomics》2005,6(2-3):113-119
We have improved our green fluorescent protein (GFP) folding reporter technology [Waldo et al., (1999) Nat. Biotechnol. 17, 691–695] to evolve recalcitrant proteins from Mycobacterium tuberculosis. The target protein is inserted into the scaffolding of the GFP, eliminating false-positive artifacts caused by expression
of truncated protein variants from internal cryptic ribosome binding sites in the target RNA. In parallel, we have developed
a new quantitative fluorescent protein tagging and detection system based on micro-domains of GFP. This split-GFP system,
which works both in vivo and in vitro, is amenable to high-throughput assays of protein expression and solubility [Cabantous et al., (2005) Nat. Biotechnol. 23, 102–107]. Together, the GFP folding reporter and split-GFP technologies offer a comprehensive system for manipulating and
improving protein folding and solubility. 相似文献
30.
《DNA Repair》2017
Cells require extra amounts of dNTPs to repair DNA after damage. Polyphosphate (polyP) is an evolutionary conserved linear polymer of up to several hundred inorganic phosphate (Pi) residues that is involved in many functions, including Pi storage. In the present article, we report on findings demonstrating that polyP functions as a source of Pi when required to sustain the dNTP increment essential for DNA repair after damage. We show that mutant yeast cells without polyP produce less dNTPs upon DNA damage and that their survival is compromised. In contrast, when polyP levels are ectopically increased, yeast cells become more resistant to DNA damage. More importantly, we show that when polyP is reduced in HEK293 mammalian cell line cells and in human dermal primary fibroblasts (HDFa), these cells become more sensitive to DNA damage, suggesting that the protective role of polyP against DNA damage is evolutionary conserved. In conclusion, we present polyP as a molecule involved in resistance to DNA damage and suggest that polyP may be a putative target for new approaches in cancer treatment or prevention. 相似文献