头花蓼和头状蓼的HPLC指纹图谱鉴定研究

建立头花蓼、头状蓼HPLC指纹图谱鉴定方法。用RP—HPLC（DAD）法,梯度洗脱,测定不同产地头花蓼及头状蓼的指纹图谱,并作相似度比较分析。不同产地头花蓼指纹图谱相似度较好,而头花蓼与头状蓼的指纹图谱有明显差异。HPLC指纹图谱具有重现性好,特征性强,方法简便等特点,可用于头花蓼、头状蓼药材的鉴别。     

虎杖中白藜芦醇、白藜芦醇甙及大黄素的综合提取工艺研究

采用HPLC法,以白藜芦醇、白藜芦醇甙及大黄素的含量为指标,对虎杖中的有效成分进行了综合提取,通过正交实验,确定出乙醇溶剂提取的最佳工艺为：以80％的乙醇水溶液为提取剂,液固比为12：1,在70℃下提取2次,每次提取2h,HPLC法测定结果表明,从虎杖中提取的有效成分的含量分别可达：白藜芦醇为2．40mg／g,白藜芦醇甙为18．12mg／g,大黄素为16．82m/g。     

Analysis of Rhizoma Polygoni Cuspidati by HPLC and HPLC-ESI/MS

An HPLC method with photodiode array detection (PAD) and ESI/MS detection was developed for the qualitative and quantitative analysis of the major chemical constituents of the dried rhizome of Polygonum cuspidatum Sieb. et Zucc. (Rhizoma Polygoni Cuspidati; Chinese name Hu-Zhang). Based on the chromatographic separation on an Altima C(18) column using 0.5% aqueous acetic acid and acetonitrile as the mobile phase, nine compounds, including stilbenes, stilbene glucosides, anthraquinones and anthraquinone glucosides, were identified by online ESI/MS analysis and seven were quantified by HPLC-PAD. A full validation of the method including sensitivity, linearity, repeatability and recovery was conducted. Linear calibration was achieved over the concentration range 1-200 mg/L with R(2) > 0.999, whilst the limits of detection ranged from 0.51 to 1.57 ng. Repeatability was evaluated by intra- and inter-day assays and the RSD value was within 1.79%. Recoveries of the quantified compounds were within the range 96.0-100.1% with RSD values of less than 2.2%. Five samples of Rhizoma Polygoni Cuspidati from different regions were analysed using the developed method. The major constituents piceid, resveratrol, emodin-8-O-beta-D-glucoside and emodin were selected to provide an index for the quality assessment of the herbal drug.     

浙江新植物

在研究浙江植物过程中, 整理出以下新植物:蔷薇科, 浙江郁李Prunus japonica Thunb.var.zhejiangensis Y.B.Chang, 蓼科, 微叶蓼Polygonum minutissimum Z.Wei et Y.B.Chang, 现发表如下。     

Effects of Temperature on the Pattern of Anthocyanin Accumulation in Seedlings of Polygonum cuspidatum

Polygonum cuspidatum seedling. Anthocyanin accumulated first in the lower part of hypocotyls and then the site of accumulation gradually extended toward the upper part of hypocotyls when seedlings were irradiated with white light (WL) at 25 C. Etiolated seedlings accumulated anthocyanin only in the upper parts (hook and cotyledons) when the seedlings were irradiated with WL at 5 C. De-etiolated seedlings that had been pre-irradiated with WL for 1 day at 25 C accumulated anthocyanin both in upper and lower parts of the seedlings when the seedlings were irradiated with WL at 5 C. Spectral sensitivity was dependent on the temperature during irradiation. Red light (R), blue light (B), and near ultra-violet light (NUV) induced the accumulation of anthocyanin at 5 C but only NUV was effective in inducing the accumulation of anthocyanin at 25 C. Dichlorophenyl dimethylurea (DCMU) inhibited WL-induced anthocyanin accumulation but did not NUV-induced anthocyanin accumulation at 25 C. However, sucrose promoted NUV action at 25 C, indicating that photosynthesis can promote NUV-induced anthocyanin accumulation. Distribution of phytochrome in etiolated seedlings, that was examined by spectrophotometry, was similar to the distribution of anthocyanin at 5 C. Furthermore, phytochrome remained after 48 hr irradiation with WL at 5 C although phytochrome was rapidly degraded at 25 C. Received 12 July 1999/ Accepted in revised form 24 December 1999     

蓼属植物的化学成分与生物活性研究进展

蓼属(Polygonum)是蓼科中的一个药用大属,我国约有120种,其中81种供药用,包括许多著名的中药和民间药。从上世纪80年代开始,对本属植物的化学成分及药理作用研究,逐渐受到了关注。本文对90年代以来,国内外对该属药用植物化学及药理活性的研究进行综述。     

人工湿地园沼泽植物水蓼(Polygonum hydropiper L.)的蒸腾特性日变化

对人工湿地园中沼泽植物水蓼(Polygonum hydropiper L.)蒸腾特性日变化进行了研究.结果表明: 水蓼叶片的蒸腾速率日进程在晴朗的天气呈单峰曲线变化,最高峰值出现在下午的14:00.受光量子通量密度的影响最大,相关系数为0.668.与相对湿度的变化呈正相关(r=0.607),说明水蓼的蒸腾速率日变化是受其自身所具有的特殊生理特性所决定.叶片气孔阻力直接影响蒸腾速率.     

西伯利亚蓼铜伴侣蛋白基因在盐胁迫条件下的表达分析

铜伴侣蛋白是细胞质中负责传递铜离子的一种小分子转运蛋白,在逆境胁迫过程中铜伴侣蛋白的ATX1家族具有消除活性氧的作用．本研究应用RACE技术从西伯利亚蓼中克隆了具有完整编码区的铜伴侣蛋白全长cDNA序列,命名为PsATX-1．PsATX-1基因全长516 bp,其中开放读码框为228 bp,编码75个氨基酸,5′非编码区为83 bp,3′非编码区为204 bp．GenBank中登录号为EU620702．经比对发现,该基因所编码蛋白拥有重金属结合位点MXCXXC,缺少多数高等植物铜伴侣蛋白(CCH)所特有的C末端结构域（CTD）．实时荧光定量PCR分析显示,PsATX-1基因在西伯利亚蓼的地下茎、茎、叶中皆有表达,其中叶中表达量最高;PsATX-1基因受3% NaHCO3胁迫诱导,在不同部位表达模式有差异．     

何首乌块根中砷、镉、汞和铅含量的检测及其富集特性

何首乌是著名传统常用中药,为蓼科(Polygonaceae)植物何首乌(Polygonum multiflorum Thunb.)的块根.生首乌具有解毒、消痈和润肠通便的作用,制首乌则有补肝肾、益精血、乌须发和强筋骨之功效[1].何首乌最早出现于唐代李翱所著的<何首乌传>,<本草图经>及<本草纲目>描述何首乌"叶叶相对",展学峰等认为二者所指为现今的萝摩科(Asclepiadaceae)植物白首乌(Cynanchum bungei Decne.)[2].何首乌野生资源分布于陕西南部、甘肃南部及华东、华中、华南和西南,日本也有分布[3]     

藏中矿区珠芽蓼和尼泊尔酸模中重金属含量分析

西藏中部主要是指雅鲁藏布江河谷地带,包括拉萨、日喀则和泽当等地市,是西藏自治区的主要农区和工业矿区。西藏中部矿产资源丰富,其中cu的远景储量居全国第2位[1]。矿产的开发给当地居民带来巨大的经济效益,但同时也排放大量含有重金属的废渣、废水和废气,导致土壤重金属污染严重。土壤是中草药中重金属的主要来源之一,中草药中的重金属含量与地质背景有密切的关系,土壤中重金属元素的多寡在药用植物中都有所表现[2];土壤重金属污染对中草药品质有影响甚至危及人类健康[3]。近年来植物药在国际市场逐渐升温,传统医药在日益受到人们青睐的同时其质量与安全性也成为公众关注的焦点[4-6]。