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991.
Synopsis Spawning of 32 species of Labridae and 13 species of Scaridae was seen at Enewetak Atoll, Marshall Islands. Most spawned on a reef bisecting the main ocean-lagoon passage which had strong tidal currents. Others spawned on lagoon reefs and in Halimeda beds. Polygynous haremic, lek-like and promiscuous mating systems were found which were species specific. Data on reproductive patterns, sexual dichromatism, sexual dimorphism, seasonality and spawning behavior were determined. Many spawned during the day in a time-phase dependent pattern from near sunrise to sunset. Scarid spawning began at slack high water or after when currents were starting to move out of the lagoon. Labrid spawning usually started about 30 min later with some continuing up to 2 h after high tide. With high tide before sunrise, scarid spawning began 30–50 min after sunrise as the current started flowing over the reef. With high tide near sunset, spawning occurred with an incoming current. Most labroids spawned on all phases of the moon. Acanthuridae (6 spp), Caesionidae (1 sp.) and Zanclidae (1 sp.) spawned after high tide at the same time as labroids. Pomacanthidae (5 spp.) spawned only shortly before sunset without reference to tidal currents. Fishes producing pelagic eggs at the lagoon-ocean channel spawned (1) at or slightly after high tide (44spp.), (2) in late afternoon without reference to tide (6 spp.) or (3) after slack low water (1 sp. ). Spawning style can vary within a single species in different environments. Despite the presence of many piscivores, no successful predation on spawning adults was seen. Predation on newly released eggs was uncommon. Labrichthys unilineata and Anampses twistii attempted to defend their eggs for a few sec after release. Attacks by piscivores on spawning adults on tropical reefs occur once per 100–1000 spawnings. Most are wary when preparing to spawn and prespawning behavior is easily interrupted. The risk from piscivores goes down and spawning ascent speed decreases with increasing size of spawners. Egg predation by zooplanktivores is less for pair spawners than group spawners possibly due to less conspicuous gamete clouds and times of spawning. Increasing height of egg release, speed and length of the spawning ascent, and trajectory alteration of ascending adults are believed to make it more difficult for zooplanktivores to locate eggs after release. For labrids, permanent full sexual dichromatism was found among haremic, lek-like and promiscuous mating systems. Species with temporary full dichromatism, permanent and temporary partial dichromatism and monochromatism were haremic. Smaller scarids were believed to have lek-like, and larger species haremic, mating systems. Smaller scarids had male looping behavior and post spawning displays, plus faster spawning ascents and different locations for egg release than larger ones. Eggs of 21 labrids were spherical or nearly spherical, ranging from 0.55–0.80 mm in diameter, and most had one oil globule. Among 7 is scarids, 6 had spindle-shaped eggs ranging from 1.25 ×0.50 mm 2.14× 0.48 mm while one had a nearly spherical egg. One scarid egg lacked an oil globule. 相似文献
992.
993.
One of the stronger empirical generalizations to emerge from the study of genetic systems is that achiasmate meiosis, which has evolved 25–30 times, is always restricted to the heterogametic sex in dioecious species, usually the male. Here we collate data on quantitative sex differences in chiasma frequency from 54 species (4 hermaphroditic flatworms, 18 dioecious insects and vertebrates and 32 hermaphroditic plants) to test whether similar trends hold. Though significant sex differences have been observed within many species, only the Liliaceae show a significant sexual dimorphism in chiasma frequency across species, with more crossing over in embryo mother cells than in pollen mother cells; chiasma frequencies are unrelated to sex and gamety in all other higher taxa studied. Further, the magnitude of sexual dimorphism, independent of sign, does not differ among the three main ecological groups (dioecious animals, plants, and hermaphroditic animals), contrary to what would be expected if it reflected sex-specific selection on recombination. These results indicate that the strong trends for achiasmate meiosis do not apply to quantitative sex differences in recombination, and contradict theories of sex-specific costs and benefits. An alternative hypothesis suggests that sex differences may be more-or-less neutral, selection determining only the mean rate of recombination. While male and female chiasma frequencies are more similar than would be expected under complete neutrality, a less absolute form of the hypothesis is more difficult to falsify. In female mice the sex bivalent has more chiasmata for its length than the autosomes, perhaps compensating for the absence of recombination in males. Finally, we observe that chiasma frequencies in males and females are positively correlated across species, validating the use of only one sex in comparative studies of recombination. 相似文献
994.
This article outlines the development and general status of present purification methods for human interferons. The isolation of each interferon species from natural sources is extremely difficult because of molecular characteristics, high losses of activity and the small amount of interferon protein present in production media. Few of the highly sophisticated methods meet the requirements for scale-up or give acceptable recoveries for a production process. The adaptation of purification procedures to the different interferon species is described, such as initial concentration, the extraction of beta interferon (IFN-β) by aqueous two-phase systems and the final purification of alpha interferon (IFN-α) and beta interferon to homogeneity. H.p.l.c. techniques are discussed in more detail together with problems in the purification of beta interferon and gamma interferon (IFN-γ). The range of interferon expression and excretion in recombinant microbial and animal cells is reviewed and different approaches for the solubilization and purification of intracellular recombinant interferons are described, which are covered mainly in patent applications. 相似文献
995.
Elis Andersson Bo Mattiasson Bärbel Hahn-Hägerdal 《Enzyme and microbial technology》1984,6(7):301-306
The conversion of benzylpenicillin (BP) to 6-aminopenicillanic acid (6-APA) using penicillin acylase (penicillin amidohydrolase, EC 3.5.1.11) has been studied in aqueous two-phase systems. In a system composed of 8.9% (w/w) PEG 20000/7.6% (w/w) potassium phosphate the enzyme was almost completely partitioned to the bottom phase (K < 0.01), which allowed repeated batch conversions, recirculating the enzyme several times. The initial specific productivities were 0.31–1.47 μmol 6-APA mg protein?1 min?1 in repeated conversions over five steps. The yield obtained from the top phase was 0.47–0.71 mol 6-APA mol BP?1. The results are discussed in relation to recirculating the enzyme by immobilizing it to a solid matrix. Despite the high phosphate concentration in the bottom phase the system needs to be titrated in order for the reaction to proceed. Titration of the top phase alone protected the enzyme from denaturation by strong alkali used for the titration. 相似文献
996.
Polar lipids such as monoglycerides are able to form an inverse micellar solution, an L2-phase, of water aggregates in triglyceride oil. This thermodynamically stable liquid phase also fulfils the criteria used to identify microemulsions. The structure of this phase at various compositions has been studied by freeze-fracture electron microscopy. The freeze-fracture images show differently oriented stacks of small smooth lamellae. These observations are consistent with X-ray studies indicating curved lipid bilayers arranged in parallel, separating similarly curved aqueous layers of finite size, forming a higly dynamic structure of ‘medium-range’ order. Studies from different compositions in the ternary systems over the L2-phase in binary monoglyceride/water systems to the pure monoglyceride in the liquid state indicate the occurrence of the same type of lamellar structure, which is proposed to be characteristic for polar lipids forming liquid-crystalline phases in contrast to less polar lipids exhibiting an amorphous structure in the liquid state. 相似文献
997.
WARREN L. KOVACH DAVID L. DILCHER F.L.S. 《Botanical journal of the Linnean Society. Linnean Society of London》1984,88(1-2):63-104
KOVACH, W. L. & DILCHER, D. L., 1984. Dispersed cuticles from the Eocene of North America. Macerations of organic-rich clay from the Claiborne Formation (Middle Eocene) of Tennessee have yielded a wide variety of well-preserved dispersed cuticles. Details of the epidermal cell patterns, arrangement of the stomata1 complex, trichomes and trichome attachment have made possible the association of some dispersed cuticles with leaf types known from this formation which have similar cuticle, and with modern families. All dispersed cuticles are classified in a morphologic system. Through both our own work and a review of previous investigations we have found that distinct dispersed cuticle types can be recognized and may be used biostratigraphically to characterize geologic strata, palaeo-ecologically to provide insights into environmental reconstruction, and systematically to follow the history of certain taxa. 相似文献
998.
A comparison of levels of genetic polymorphism and self-compatibility in geographically restricted and widespread plant congeners 总被引:11,自引:0,他引:11
Jeffrey D. Karron 《Evolutionary ecology》1987,1(1):47-58
Summary Evolutionary theory predicts low levels of genetic polymorphism and high levels of self-compatibility in plant species with small ranges and few individuals. To test these predictions, I compared published data on electrophoretically detectable genetic variation and breeding systems for geographically restricted and widespread congeners in eleven genera. The restricted species exhibit significantly fewer polymorphic loci and alleles per polymorphic locus than do their widespread congeners. Although some rare species are genetically impoverished, others are nearly as polymorphic as their widespread congeners. The restricted species and their widespread congeners do not differ consistently with respect to breeding systems. 相似文献
999.
A method for the purification of proteins hybridized with β-galactosidase and produced in Escherichia coli is suggested. The method is based on the dominating properties of the β-galactosidase part of the molecule that are utilized for extraction in a poly(ethylene glycol) 4000/potassium phosphate aqueous two-phase system. The purification of the hybrid protein Staphylococcal protein A-Escherichia coli-β-galactosidase (SpA-βgal) produced in Escherichia coli is described. The partitioning of the cell debris and SpA-βgal depended on the distance to the critical point, i.e., the length of the tie line. A poly(ethylene glycol) top phase and an interface free from cell debris were obtained for a composition close to the binodial with a relatively short tie line. At this composition no Spa-βgal partitioned to the interface. When the length of the tie line was increased, more of the SpA-βgal was caught by the interface. The partitioning of SpA-βgal to the top phase was also affected by the salts present during the extraction. The utilization of SpA-βgal for affinity extraction has been investigated. Experiments with SpA-βgal and fluorescence-labeled human IgG(hIgG-F) in a poly(ethylene glycol) 4000/potassium phosphate aqueous two-phase system showed that the complex SpA-βgal-hlgG-F was partitioned to the interface, probably as a precipitate. 相似文献
1000.
Two closely related species ofCapparis, C. ovata andC. spinosa, which are exposed to the same pollination environment were studied. The nectar volume and concentration ofC. ovata are higher than inC. spinosa. Both species have similar pollination efficiency, but the relative contribution of each pollinator is different.C. ovata is pollinated mainly hy hawkmoths while the principal pollinators inC. spinosa are bees. 相似文献