全文获取类型
收费全文 | 11810篇 |
免费 | 918篇 |
国内免费 | 436篇 |
出版年
2024年 | 13篇 |
2023年 | 162篇 |
2022年 | 115篇 |
2021年 | 243篇 |
2020年 | 279篇 |
2019年 | 366篇 |
2018年 | 302篇 |
2017年 | 387篇 |
2016年 | 341篇 |
2015年 | 382篇 |
2014年 | 398篇 |
2013年 | 539篇 |
2012年 | 380篇 |
2011年 | 393篇 |
2010年 | 325篇 |
2009年 | 577篇 |
2008年 | 604篇 |
2007年 | 714篇 |
2006年 | 599篇 |
2005年 | 539篇 |
2004年 | 468篇 |
2003年 | 420篇 |
2002年 | 377篇 |
2001年 | 331篇 |
2000年 | 339篇 |
1999年 | 330篇 |
1998年 | 345篇 |
1997年 | 271篇 |
1996年 | 302篇 |
1995年 | 277篇 |
1994年 | 203篇 |
1993年 | 221篇 |
1992年 | 202篇 |
1991年 | 186篇 |
1990年 | 169篇 |
1989年 | 171篇 |
1988年 | 145篇 |
1987年 | 124篇 |
1986年 | 96篇 |
1985年 | 107篇 |
1984年 | 88篇 |
1983年 | 41篇 |
1982年 | 61篇 |
1981年 | 50篇 |
1980年 | 47篇 |
1979年 | 34篇 |
1978年 | 33篇 |
1977年 | 20篇 |
1976年 | 16篇 |
1973年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 171 毫秒
991.
992.
Kuchel H Williams KJ Langridge P Eagles HA Jefferies SP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(8):1029-1041
Grain yield forms one of the key economic drivers behind a successful wheat (Triticum aestivum L.) cropping enterprise and is consequently a major target for wheat breeding programmes. However, due to its complex nature,
little is known regarding the genetic control of grain yield. A doubled-haploid population, comprising 182 individuals, produced
from a cross between two cultivars ‘Trident’ and ‘Molineux’, was used to construct a linkage map based largely on microsatellite
molecular makers. ‘Trident’ represents a lineage of wheat varieties from southern Australia that has achieved consistently
high relative grain yield across a range of environments. In comparison, ‘Molineux’ would be rated as a variety with low to
moderate grain yield. The doubled-haploid population was grown from 2002 to 2005 in replicated field experiments at a range
of environments across the southern Australian wheat belt. In total, grain yield data were recorded for the population at
18 site-year combinations. Grain yield components were also measured at three of these environments. Many loci previously
found to be involved in the control of plant height, rust resistance and ear-emergence were found to influence grain yield
and grain yield components in this population. An additional nine QTL, apparently unrelated to these traits, were also associated
with grain yield. A QTL associated with grain yield on chromosome 1B, with no significant relationship with plant height,
ear-emergence or rust resistance, was detected (LOD ≥2) at eight of the 18 environments. The mean yield, across 18 environments,
of individuals carrying the ‘Molineux’ allele at the 1B locus was 4.8% higher than the mean grain yield of those lines carrying
the ‘Trident’ allele at this locus. Another QTL identified on chromosome 4D was also associated with overall gain yield at
six of the 18 environments. Of the nine grain yield QTL not shown to be associated with plant height, phenology or rust resistance,
two were located near QTL associated with grain yield components. A third QTL, associated with grain yield components at each
of the environments used for testing, was located on chromosome 7D. However, this QTL was not associated with grain yield
at any of the environments. The implications of these findings on marker-assisted selection for grain yield are discussed. 相似文献
993.
Calculations to derive effective population size become highly complicated when complex population structure is considered. We provide an easy method of computing the effective size of a subdivided population with overlapping generations (a spatiotemporally structured population) using an approximation based on separation of time scales. We also numerically compute the effective size to verify the accuracy of the derived formula. Various interesting quantities, including moments of coalescent time, are readily derived using this approach. 相似文献
994.
Gap junctions of some vertebrates are capable of passing the elongate molecule, calmodulin, with a molecular weight 8-17 times greater than the previously recognized size limits. Fluorescently labeled calmodulin (FCaM) (17.34 kDa) microinjected into oocytes of ovarian follicles from an amphibian, Xenopus laevis, and from two species of teleost fish, Danio rerio (Zebrafish) and Oryzias latipes (Medaka), is shown to transit their gap junctions and enter the surrounding epithelial cells. Passage of FCaM was terminated when follicles were first treated with 1 mM octanol, a molecule known to down-regulate gap junctions. There was no FCaM detected in the surrounding medium, nor did epithelial cells become fluorescent when follicles were incubated in medium containing dye. Calmodulin is well known to modulate many cytoplasmic reactions; thus, its passage through gap junctions opens possibilities of additional means by which cells may be supplied with this signaling molecule, and by which their supply may be regulated. 相似文献
995.
Reichert J Grasnick D Afonin S Buerck J Wadhwani P Ulrich AS 《European biophysics journal : EBJ》2007,36(4-5):405-413
It is generally assumed that fusogenic peptides would require a certain conformation, which triggers or participates in the
rate-determining step of membrane fusion. Previous structure analyses of the viral fusion peptide from gp41 of HIV-1 have
yielded contradictory results, showing either an α-helical or a β-stranded conformation under different conditions. To find
out whether either of these conformations is relevant in the actual fusion process, we have placed sterically demanding substitutions
into the fusion peptide FP23 to prevent or partially inhibit folding and self-assembly. A single substitution of either D- or L-CF3-phenylglycine was introduced in different positions of the sequence, and the capability of these peptide analogues to fuse
large unilamellar vesicles was monitored by lipid mixing and dynamic light scattering. If fusion proceeds via a β-stranded
oligomer, then the D- and L-epimers are expected to differ systematically in their activity, since the D-epimers should be unable to form β-structures due to sterical hindrance. If an α-helical conformation is relevant for fusion,
then the D-epimers would be slightly disfavoured compared to the L-forms, hence a small systematic difference in fusion activity should be observed. Interestingly, we find that (1) all D- and L-epimers are fusogenically active, though to different extents compared to the wild type, and – most importantly – (ii) there
is no systematic preference for either the D- or L-forms. We therefore suggest that a well-structured α-helical peptide conformation or a β-stranded oligomeric assembly can
be excluded as the rate-determining state. Instead, fusion appears to involve conformationally disordered peptides with a
pronounced structural plasticity.
Dedicated to Prof. K. Arnold on the occasion of this 65th birthday. 相似文献
996.
997.
Qazi O Bolgiano B Crane D Svergun DI Konarev PV Yao ZP Robinson CV Brown KA Fairweather N 《Journal of molecular biology》2007,365(1):123-134
Protein oligomerisation is a prerequisite for the toxicity of a number of bacterial toxins. Examples include the pore-forming cytotoxin streptolysin O, which oligomerises to form large pores in the membrane and the protective antigen of anthrax toxin, where a heptameric complex is essential for the delivery of lethal factor and edema factor to the cell cytosol. Binding of the clostridial neurotoxins to receptors on neuronal cells is well characterised, but little is known regarding the quaternary structure of these toxins and the role of oligomerisation in the intoxication process. We have investigated the oligomerisation of the receptor binding domain (H(C)) of tetanus toxin, which retains the binding and trafficking properties of the full-length toxin. Electrophoresis, size exclusion chromatography and mass spectrometry were used to demonstrate that H(C) undergoes concentration-dependent oligomerisation in solution. Reducing agents were found to affect H(C) oligomerisation and, using mutagenesis, Cys869 was shown to be essential for this process. Furthermore, the oligomeric state and quaternary structure of H(C) in solution was assessed using synchrotron small-angle X-ray scattering. Ab initio shape analysis and rigid body modelling coupled with mutagenesis data allowed the construction of an unequivocal model of dimeric H(C) in solution. We propose a possible mechanism for H(C) oligomerisation and discuss how this may relate to toxicity. 相似文献
998.
Human Hint3 (hHint3) has been classified as a member of the histidine triad nucleotide (Hint) binding protein subfamily. While Hint1 is ubiquitously expressed by both eukaryotes and prokaryotes, Hint3 is found only in eukaryotes. Previously, our laboratory has characterized and compared the aminoacyl-adenylate and nucleoside phosphoramidate hydrolase activity of hHint1 and Escherichia coli hinT. In this study, hHint3-1(Ala36) and its single nucleotide polymorphism, hHint3-2 (A36G variant), were cloned, overexpressed, and purified. Steady-state kinetic studies with a synthetic fluorogenic indolepropinoic acyl-adenylate (AIPA) and with a series of fluorogenic tryptamine nucleoside phosphoramidates revealed that hHint3-1 and hHint3-2 are adenylate and phosphoramidate hydrolases with apparent second-order rate constants (kcat/Km) ranging from 10(2) to 10(6) s(-1) M(-1). Unlike hHint1, hHint3-1 and hHint3-2 prefer AIPA over tryptamine adenosine phosphoramidate by factors of 33- and 16-fold, respectively. In general, hHint3s hydrolyze phosphoramidate 370- to 2000-fold less efficiently than hHint1. Substitution of the potential active-site nucleophile, His145, by Ala was shown to abolish the adenylate and phosphoramidate hydrolase activity for hHint3-1. However, 0.2-0.4% residual activity was observed for the H145A mutant of hHint3-2. Both hHint3-1 and hHint3-2 were found to hydrolyze lysyl-adenylate generated by human lysyl-tRNA synthetase (hLysRS) by proceeding through an adenylated protein intermediate. hLysRS-dependent labeling of hHint3-1 and hHint3-2 was found to depend on His145, which aligns with the His112 of the Hint1 active site. The extent of active-site His145-AMP labeling was shown to be similar to His112-AMP labeling of hHint1. In contrast to all previously characterized members of the histidine triad superfamily, which have been shown to exist exclusively as homodimers, wild type and the H145A of hHint3-1 were found to exist across a range of multimeric states, from dimers to octamers and even larger oligomers, while wild type and the H145A of hHint3-2 exist predominantly in a monomeric state. The differences in oligomeric state may be important in vivo, because unlike tetracysteine-tagged Hint1, which was found along linear arrays exclusively in the cytoplasm in transfected HeLa cells, tagged Hint3-1 and Hint3-2 were found as aggregates both in the cytosol and in the nucleus. Taken together, these results imply that while Hint3 and Hint1 prefer aminoacyl-adenylates as substrates and catalytically interact with aminoacyl-tRNA synthetases, the significant differences in phosphoramidase activity, oligomeric state, and cellular localization suggest that Hint3s should be placed in a distinct branch of the histidine triad superfamily. 相似文献
999.
We carried out a theoretical investigation of whether ecological character displacement can be caused by reproductive interference. Our model assumes that a quantitative character is associated with both resource use and species recognition, and that heterospecific mating incurs costs. The model shows that ecological character displacement can occur as a consequence of evolution of premating isolation; this conclusion is based on the premise that resource competition is less intense between species than within species and that the ecological character also contributes to premating isolation. When resource competition between species is intense, extinction of either species may occur by competitive exclusion before ecological character divergence. Some observational studies have shown that character displacement in body size is associated with not only resources use but also species recognition. We propose that body size displacement can occur as a consequence of evolution of premating isolation. Our results suggest that ecological character displacement results from reproductive character displacement. 相似文献
1000.
Conservation programs that deal with small or declining populations often aim at a rapid increase of population size to above-critical levels in order to avoid the negative effects of demographic stochasticity and genetic problems like inbreeding depression, fixation of deleterious alleles, or a general loss of genetic variability and hence of evolutionary potential. In some situations, population growth is determined by the number of females available for reproduction, and manipulation of family sex ratios towards more daughters has beneficial effects. If sex determination is predominantly genetic but environmentally reversible, as is the case in many amphibia, reptiles, and fish, Trojan sex chromosomes could be introduced into populations in order to change sex ratios towards more females. We analyse the possible consequences for the introduction of XX-males (XX individuals that have been changed to phenotypic males in a XY/XX sex determination system) and ZW males, WW males, or WW females (in a ZZ/ZW sex determination system). We find that the introduction of WW individuals can be most effective for an increase of population growth, especially if the induced sex change has little or no effect on viability. 相似文献