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81.
流行性乙型脑炎活疫苗细胞免疫检测方法的探索 总被引:1,自引:0,他引:1
本文建立了流行性乙型脑炎活疫苗细胞免疫检测方法。采用纯系Balb/c(H-2d)小鼠制备效应细胞,以同一品系Balb/c(H-2d)小鼠原代肾细胞为靶细胞。当效靶细胞比例为20∶1,杀伤时间为4小时,MTT反应时间为4小时,细胞毒性T淋巴细胞(CTL)杀伤作用最强。利用该法对乙脑活苗和死苗免疫小鼠进行了CTL活性测定,结果表明该法重复性好,活苗诱导CTL杀伤作用强于死苗 相似文献
82.
Beatris Mastelic Nathalie Garçon Giuseppe Del Giudice Hana Golding Marion Gruber Pieter Neels Bernard Fritzell 《Biologicals》2013,41(6):458-468
Vaccination represents one of the greatest public health triumphs; in part due to the effect of adjuvants that have been included in vaccine preparations to boost the immune responses through different mechanisms. Although a variety of novel adjuvants have been under development, only a limited number have been approved by regulatory authorities for human vaccines. This report reflects the conclusions of a group of scientists from academia, regulatory agencies and industry who attended a conference on the current state of the art in the adjuvant field. Held at the U.S. Pharmacopeial Convention (USP) in Rockville, Maryland, USA, from 18 to 19 April 2013 and organized by the International Association for Biologicals (IABS), the conference focused particularly on the future development of effective adjuvants and adjuvanted vaccines and on overcoming major hurdles, such as safety and immunogenicity assessment, as well as regulatory scrutiny. More information on the conference output can be found on the IABS website, http://www.iabs.org/. 相似文献
83.
Takata T Shirakawa T Kawasaki Y Kinoshita S Gotoh A Kano Y Kawabata M 《The journal of gene medicine》2006,8(11):1341-1346
BACKGROUND: A critical component of the host defense against enteric infections is the immunological response of the mucosal membrane, a major starting point of infectious disease, such as typhoid fever. The mucosal immune system consists of an integrated network of lymphoid tissues, mucous membrane-associated cells, and effector molecules. In the present study, we developed a recombinant Bifidobacterium animalis (B. animalis) genetically modified with the Salmonella flagellin gene for mucosal immunization as an oral typhoid vaccine. METHODS: We constructed an oral vaccine against Salmonella typhimurium, consisting of recombinant B. animalis containing the flagellin gene of Salmonella. The recombinant B. animalis was administered orally to mice every other day for 6 weeks. Anti-flagellin antibodies in the serum and stools were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: We detected significantly higher levels of flagellin-specific IgA in the serum and stools of the mice treated with the recombinant B. animalis containing the flagellin gene than was seen in those treated with parental B. animalis. CONCLUSIONS: Our findings suggest that an oral vaccination using recombinant B. animalis genetically modified with the flagellin gene of Salmonella may be effective against Salmonella infections. 相似文献
84.
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86.
沪191麻疹疫苗免疫持久性和影响因素的评价 总被引:11,自引:0,他引:11
1991~1998年,我们对荆州区川店镇503名6~15月龄儿童进行了现行沪191麻疹疫苗血清流行病学效果观察,结果表明,初次免疫后1个月麻疹IgG抗体阳转率为9165%,GMT为1∶26674,达保护滴度者比例为465%。随着时间的推移,第4年上述指标迅速下降到4686%、1∶1274和185%,第6年时低至2943%、1∶489和136%。02ml、03ml和05ml麻疹疫苗组的近期和远期效果是类似的,初免后1个月时IgG滴度越高,其免疫持久性越好;初免月龄是影响麻苗免疫效果的主要原因,6月龄初免组的免疫效果明显低于≥8月龄组。结果提示麻苗8月龄初免是可行的。 相似文献
87.
Macmillan L Ifere GO He Q Igietseme JU Kellar KL Okenu DM Eko FO 《FEMS immunology and medical microbiology》2007,49(1):46-55
Chlamydia trachomatis and Herpes simplex virus type 2 (HSV-2) genital infections pose a considerable public health challenge worldwide. Considering the high incidence of coinfections by the two pathogens, a combination vaccine that can be administered as a single regimen would be highly desirable. Recombinant Vibrio cholerae ghosts (rVCG) offer an attractive approach for the induction of humoral and cellular immune responses against human and animal pathogens. In this study, we evaluated a bivalent combination vaccine formulation comprising rVCG expressing chlamydial MOMP and HSV-2 glycoprotein D in mice for immunogenicity and protective efficacy against genital challenge with either pathogen. Mice immunized with the combination vaccine elicited secretory IgA and IgG2a antibodies to both chlamydial and HSV-2 antigens in serum and vaginal secretions. Robust antigen-specific mucosal and systemic T helper type 1 responses were induced in mice as measured by increased interferon-gamma levels produced by immune T cells in response to restimulation with target antigen in vitro. In addition, mice immunized with the combination vaccine were prophylactically protected from genital challenge with high doses of live Chlamydia and HSV-2. Thus, the combination vaccine regimen delivered by rVCG elicited adequate immune effectors that simultaneously protected against the individual pathogens. 相似文献
88.
Zanotto C Elli V Basavecchia V Brivio A Paganini M Pinna D Vicenzi E De Giuli Morghen C Radaelli A 《FEMS immunology and medical microbiology》2003,35(1):59-65
Two different prime-boost immunization protocols were tested in rabbits and their immune response was evaluated and compared with the final aim of defining a vaccine strategy that might be able to protect non-human primates from infection with the pathogenic simian/human immunodeficiency virus, SHIV(89.6P). The two regimens were based on three priming immunizations with either an expression plasmid plus a fowlpox (FP) recombinant vector or with two FP recombinant vectors, each one expressing either the SIV(mac239) gag/pol or the HIV-1env(89.6P) genes. In both protocols, priming immunizations were followed by two boosts with SHIV-mimicking virus-like particles (VLP). A complete SHIV-specific response was observed in all animals. Interestingly, the DNA vaccine was three to 10 times more efficient than the FP recombinant in inducing an anti-gag humoral response. Real-time PCR confirmed the memory effect on T-cell subsets secreting interleukin-4 and interferon-gamma, as a consequence of stimulation of both arms of the immune system. Although both protocols were almost equally effective in eliciting homologous neutralizing antibodies and highlighted the efficacy of VLP administration for boosting, protocol A seemed to be more effective in promoting a balanced T-cell memory immune response and appears more promising for vaccine purposes. 相似文献
89.
Guirola M Urquiza D Alvarez A Cannan-Haden L Caballero E Guillén G 《FEMS immunology and medical microbiology》2006,46(2):169-179
In this study, we used an adoptive lymphocyte transfer experiment to evaluate the ability of the P64k recombinant protein to recruit T-helper activity and induce immunologic memory response to the polysaccharide moiety in a meningococcal serogroup C conjugate vaccine. Adoptive transfer of splenocytes from mice immunized with the glycoconjugate conferred antipolysaccharide immunologic memory to naive recipient mice. The observed anamnestic immune response was characterized by more rapid kinetics, isotype switching from IgM to IgG and higher antipolysaccharide antibody titers compared with those reached in groups transferred with splenocytes from plain polysaccharide or phosphate-immunized mice. The memory response generated was also long lasting. Sera from mice transferred with cells from conjugate-immunized mice were the only protective in the infant rat passive protection assay, and also showed higher bactericidal titers. We demonstrated that priming the mice immune system with the glycoconjugate using the P64k protein as carrier induced a memory response to the polysaccharide, promoting a switch of the T-cell-independent response to a T-cell dependent one. 相似文献
90.
猪圆环病毒2型-细小病毒-伪狂犬重组病毒免疫小鼠试验研究 总被引:1,自引:0,他引:1
为了研究表达猪2型圆环病毒ORF2基因和猪细小病毒VP2基因的重组伪狂犬病毒疫苗株SA215(D)的疫苗价值,对该病毒株安全性、免疫原性、保护力及对PRV Fa株定值进行了研究。研究结果表明重组病毒SA215(D)毒力显著低于PRV Fa强毒株,可以抵御伪狂犬病强毒Fa株的攻击,并能抵御其在小鼠体内的定植;抗体检测显示SA215(D)免疫小鼠后第2周抗PRV、PCV2和PPV的抗体水平均很低,加强免疫后第4周抗体水平大幅上升,且显著高于阴性对照组,而与阳性对照组无明显差异,表明SA215(D)可诱导小鼠产生体液免疫反应。淋巴细胞增殖试验显示SA215(D)可诱导小鼠产生较强的淋巴细胞增殖反应,与亲本株SA215组相比,差异显著(0.01 相似文献