全文获取类型
收费全文 | 1326篇 |
免费 | 60篇 |
国内免费 | 68篇 |
专业分类
1454篇 |
出版年
2023年 | 11篇 |
2022年 | 17篇 |
2021年 | 24篇 |
2020年 | 30篇 |
2019年 | 21篇 |
2018年 | 24篇 |
2017年 | 18篇 |
2016年 | 28篇 |
2015年 | 41篇 |
2014年 | 57篇 |
2013年 | 51篇 |
2012年 | 50篇 |
2011年 | 36篇 |
2010年 | 29篇 |
2009年 | 39篇 |
2008年 | 58篇 |
2007年 | 57篇 |
2006年 | 46篇 |
2005年 | 60篇 |
2004年 | 50篇 |
2003年 | 45篇 |
2002年 | 38篇 |
2001年 | 56篇 |
2000年 | 48篇 |
1999年 | 26篇 |
1998年 | 42篇 |
1997年 | 32篇 |
1996年 | 43篇 |
1995年 | 36篇 |
1994年 | 28篇 |
1993年 | 29篇 |
1992年 | 36篇 |
1991年 | 24篇 |
1990年 | 31篇 |
1989年 | 23篇 |
1988年 | 27篇 |
1987年 | 19篇 |
1986年 | 20篇 |
1985年 | 16篇 |
1984年 | 20篇 |
1983年 | 5篇 |
1982年 | 13篇 |
1981年 | 15篇 |
1980年 | 12篇 |
1979年 | 10篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1972年 | 1篇 |
排序方式: 共有1454条查询结果,搜索用时 15 毫秒
31.
Genetic markers for Atlantic salmon (Salmo salar L.): single locus inheritance and joint segregation analyses of minisatellite (VNTR) DNA loci 总被引:1,自引:0,他引:1
Relatively few genetic markers are available for detailed studies of Atlantic salmon. The detection of 12 distinct minisatellite DNA loci in this species (by 10 Atlantic salmon and brown trout derived probes) and subsequent inheritance analyses in two half-sib families are reported here. Disomic Mendelian inheritance was confirmed at all loci. Only a single aberrant progeny genotype (at Ssa -A60) was observed among 138 progeny screened. None of the loci was sex-linked. The tight linkage association Str -A22/1 with Str -A22/2, previously reported for brown trout, was found to be conserved in the Atlantic salmon genome. An additional male-specific linkage group, Ssa -A34 with Str -A9/2, was also noted. These highly polymorphic loci should find widespread use as chromosomal, individual, familial and, probably, population markers. 相似文献
32.
Leonne M. van der Leede-Plegt Bernadette C. E. van de Ven Mirjam Schilder John Franken Arjen J. van Tunen 《Transgenic research》1995,4(2):77-86
The development is described of a new procedure to genetically transform plant species using the male gametophyte as a natural transformation vector. Our system avoids the need for complicated regeneration procedures thus making it broadly applicable. Naked plasmid DNA encoding kanamycin resistance and GUS activity was introduced by particle gun bombardment into mature pollen grains ofNicotiana glutinosa. Bombarded pollen was used for pollinations and the resulting seeds were selected for kanamycin resistance. Two different kanamycin-resistant plants, designated VIP A and VIP B, were obtained in two independent experiments. In VIP A, TR2-driven GUS activity was observed in vascular bundles, trichomes and in a small number of pollen grains. DNA gel blot analysis indicated that the introduced DNA was integrated independently into the genome of VIP A and VIP B. It was shown that male and female gametophyte development and seed set were highly aberrant in both VIP A and VIP B and that the offspring of self- and cross-pollinations did not contain the transgenes. This might be caused by a recombination event during the integration of the naked DNA resulting in a deletion of part of the target chromosome. After meiosis such a deletion is lethal for the gametes. Our observation that the transgenes were detected in DNA isolated from sporophytic tissues but not in DNA from VIP A and VIP B pollen grains is in line with this explanation. Future experiments designed to increase the frequency of transformation and to transfer the transgenes to the offspring are discussed. 相似文献
33.
Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence 总被引:11,自引:0,他引:11
Pollen grains of Plumbago zeylanica L. were serially sectioned and examined using transmission electron microscopy to determine the three-dimensional organization of sperm cells within the microgametophyte and the quantity of membrane-bound organelles occurring within each cell. Sperm cells occur in pairs within each pollen grain, but are dimorphic, differing in size, morphology and organelle content. The larger of the two sperm cells (Svn) is distinguished by the presence of a long (approx. 30 m) projection, which wraps around and lies within embayments of the vegetative nucleus. This cell contains numerous mitochondria, up to two plastids and, infrequently, microbodies. It is characterized by a larger volume and surface area and contains a larger nucleus than the other sperm cell. The second sperm cell (Sua) is linked by plasmodesmata with the Svn, but is unassociated with the vegetative nucleus. It is smaller and lacks a cellular projection. The Sua contains relatively few mitochondria, but numerous (up to 46) plastids and more microbodies than the other sperm. The degree of dimorphism in their content of heritable cytoplasmic organelles must at fertilization result in nearly unidirectional transmission of sperm plastids into just one of the two female reproductive cells, and preferential transmission of sperm mitochondria into the other.Abbreviations Sua
sperm cell unassociated with the vegetative nucleus
- Svn
sperm cell physically associated with the vegetative nucleus
1=Russell and Cass (1981) 相似文献
34.
G. P. Wagner 《Journal of mathematical biology》1984,21(1):77-95
A quantitative genetic model of random pleiotropy is introduced as reference model for detecting the kind and degree of organization in quantitative genetic variation. In this model the genetic dispersion matrix takes the form of G = BB
T, where B is a general, real, Gaussian random matrix. The eigenvalue density of the corresponding ensemble of random matrices (G) is considered. The first two moments are derived for variance-covariance matrices G as well as for correlation matrices R, and an approximate expression of the density function is given. The eigenvalue distribution of all empirical correlation matrices deviates from that of a random pleiotropy model by a very large leading eigenvalue associated with a size factor. However the frequency-distribution of the remaining eigenvalues shows only minor deviations in mammalian skeletal data. A prevalence of intermediate eigenvalues in insect data may be caused by the inclusion of many functionally unrelated characters. Hence two kinds of deviations from random organization have been found: a mammal like and an insect like organization. It is concluded that functionally related characters are on the average more tightly correlated than by chance (= mammal like organization), while functionally unrelated characters appear to be less correlated than by random pleiotropy (insect like organization). 相似文献
35.
The esterases of the cotton boll weevil were separated by polyacrylamide gel electrophoresis into four major regions. These were named Est I–IV in order of migration from anode to origin. Polymorphism was observed in all regions. The Est II region was shown to consist of no more than two bands (fast and slow). The inheritance of the fast and slow bands of Est II was demonstrated to be controlled by codominant autosomal alleles. Analysis of the gene frequency of the Est II region showed that one field population was consistent with the Hardy-Weinberg law (P=0.995), while a second field population was not at equilibrium (P<0.001).This work was supported in part by a Faculty Research Grant from Memphis State University. 相似文献
36.
S. R. Sykes 《Plant and Soil》1992,146(1-2):123-129
Citrus and grapevines are salt-sensitive perennial crops. Damage caused by salinity is due mostly to accumulation of excessive concentrations of salt (Na- and Cl ions) in shoot tissues. In both crops, however, some rootstock varieties can restrict the accumulation of salt in scion leaves and stems. Salt-excluding rootstocks, however, are often deficient with regard to other desirable characteristics and as such their use is limited. As a consequence, we have conducted a range of crosses within both crops to select new salt-excluding hybrids which may have potential as new rootstocks and also to investigate the inheritance of salt exclusion in these woody perennials.In citrus, both Cl-ion and Na-ion exclusion has been observed as a continuous character and progenies segregate widely for their ability to restrict the accumulation of these ions in shoot tissues. The ability to exclude Cl ions appears to be independent of the ability to exclude Na ions. Thus a good Cl-ion excluder is not necessarily a good Na-ion excluder and vice versa. It has been possible, however, to select new salt-excluding citrus hybrids which perform as well as and sometimes better than parent varieties when grafted with a common scion and grown in artificially salinised field plots.In grapevines, the research has concentrated on the ability for Cl-ion exclusion and depending on the Cl-ion-excluding parent chosen this is inherited as either a polygenic or monogenic trait. In crosses between Vitis champini (Cl-ion excluder) and Vitis vinifera (Cl-ion accumulator), the ability to restrict Cl-ion accumulation in shoot tissues segregates widely with some offspring transgressing the performance of either parent. In crosses and backcrosses involving V. berlandieri and V. vinifera, however, hybrids segregate as either Cl-ion excluders or accumulators suggesting the effect of a major dominant gene for Cl-ion exclusion from V. berlandieri. This was evident from both field and glasshouse experiments although possible modifying genes from V. vinifera may affect the expression of this gene under glasshouse conditions in some crosses. 相似文献
37.
C. A. Hackett R. P. Ellis B. P. Forster J. W. McNicol M. Macaulay 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,85(1):120-126
Summary The quantitative traits height and ear-emergence date were analyzed in the F2 progeny of a cross between a tall winter barley cultivar (Gerbel) and a short spring barley cultivar (Heriot). The trait distributions were found to be related to the genotypes at two biochemical loci, -amylase (Bmy1) and water-soluble protein (Wsp3), which are known to lie on the long arm of chromosome 4. Linkages between each trait and the markers were investigated using normal mixture models. The two parental phenotypes and the heterozygote phenotype of Bmy1 were distinguishable so the model could be used directly to estimate linkage between Bmy1 and a quantitative trait locus (QTL) for height (Height). The Gerbel homozygote and heterozygote phenotype of Wsp3 could not be distinguished and the model was adapted accordingly. The proportion of plants requiring vernalization was consistent with control by two independent genes acting epistatically, and a normal mixture model based on a two-gene hypothesis was fitted to the distribution of ear-emergence date to estimate linkage between the marker loci and a QTL for ear-emergence date (Vrn1). The parameters of each model were the recombination fraction between the marker locus and the QTL and the means and standard deviations associated with each QTL genotype; these were estimated by maximum likelihood. The fitted distributions correspond well to those observed and the order of the loci along the chromosome is inferred to be Height — Vrn1 — Bmy1 — Wsp3, with Wsp3 being the most distal. 相似文献
38.
Cultural transmission and the evolution of cooperative behavior 总被引:5,自引:0,他引:5
Sociobiological theory predicts that humans should not cooperate with large groups of unrelated individuals. This prediction is based on genetic models that show that selection acting on variation between large unrelated groups will generally be much weaker than selection acting on variation between individuals. Recently, several authors have presented related models of human evolution that integrate cultural and genetic transmission of behavior. We show that in such models group selection is potentially a strong force. Data on ethnocentrism is examined in the context of these results. 相似文献
39.
Gerhard Link 《Planta》1982,154(1):81-86
The steady-state levels of plastid RNA sequences in dark-grown and light-grown mustard (Sinapis alba L.) seedlings have been compared. Total cellular RNAs were labeled in vitro with 32P and hybridized to separated restriction fragments of plastid DNA. Cloned DNA fragments which encode the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxylase (dimerizing), EC 4.1.1.39] and a 35,000 plastid polypeptide were used as probes to assess the levels of these two plastid mRNAs. The 1.22-kilobase-pair mRNA for the 35,000 polypeptide is almost undetectable in dark-grown seedlings, but is a major plastid mRNA in light-grown seedlings. The hybridization analysis of RNA from seedlings which were irradiated with red and far-red light indicates that the level of this mRNA, but not of LS mRNA, is controlled by phytochrome.Abbreviations LS
large subunit
- RuBP
ribulose-1,5-bisphosphate
- ptDNA
plastid DNA 相似文献
40.
R. A. E. Tilney-Bassett Dr. O. A. L. Abdel-Wahab 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,62(2):185-191
Summary Two distinct segregation patterns are recognized after G X W plastid crosses in Pelargonium. Type I parents produce offspring in which maternal zygotes are frequent, biparental intermediate, and paternal zygotes rare (MZ>BPZ>PZ), as defined by the presence or absence of green or white plastids in the young embryos into which the zygotes develop. Type II parents produce offspring in which maternal and paternal zygotes are frequent with biparental zygotes the least frequent class (MZ>BPZPr
1
Pr
1. Type II plants, which do not breed true, are regarded as heterozygotes — Pr
1
Pr
2. The nuclear gene is symbolized as Pr as it is presumed to control alternative patterns of plastid segregation through an effect on plastid replication.Selfs and intercrosses of heterozygous plants segregate in an unexpected 1:1 ratio and not the expected 3:1 (1:2:1). The alternative homozygote — Pr
2
Pr
2 — could not be detected. Reciprocal crosses between heterozygotes (Pr
1
Pr
2) and homozygotes (Pr
1
Pr
1) give the expected 1:1 ratio when the Pr
2 allele is derived from the male, whereas there is often, but not always, a highly significant deviation from 1:1 when the Pr
2 allele is derived from the female.A simple explanation, which is not wholly satisfactory, is to assume that Pr
2 is a gametophytic lethal on the female side. An alternative, or additional, explanation is that an incompatibility mechanism is involved in which Pr
1 is a self-compatible allele, Pr
2 a self-incompatible allele, and Pr
1-Pr
2 cross-compatible alleles. Successful fertilization is then determined by sporophytic control on the male side and gametophytic control on the female side. 相似文献