基于社会-生态系统的沙漠化逆转过程脆弱性评价指标体系

土地沙漠化是当今世界最为严峻的生态环境问题之一。沙漠化逆转作为其反向转化过程,存在一定的脆弱性和不稳定性,即沙漠化逆转趋势减弱或反向发展的倾向。社会-生态系统理念是当今世界生态系统分析的新思路,从该视角界定沙漠化逆转过程的脆弱性概念,选取Driving-Pressure-Status-Impact-Response(DPSIR)分析框架,以农牧交错带典型沙漠化逆转区宁夏盐池县为案例,针对沙漠化逆转的因果联系和过程特点,构建一套涵盖气候湿润指数、生态治理指数、沙地所占比例在内的4层、5要素、41指标的评价体系,进而探讨驱动力、压力、状态、影响、响应5项要素间的逻辑因果关系及其脆弱性定量化计算方法,以期科学评估沙漠化逆转过程的脆弱性,为该类型区沙漠化问题的进一步治理和调控提供科学依据。     

一种基于SWAT模型的干旱牧区生态脆弱性评价方法——以艾布盖河流域为例

针对干旱牧区生态脆弱性特点,以GIS为平台,利用气象、土壤、土地利用、水资源开发利用等数据,结合SWAT模型模拟结果,建立了一种基于SWAT模型子流域划分的干旱牧区生态脆弱性评价体系,对西北干旱牧区艾布盖河流域1990年、2010年生态脆弱性进行了评价。从评价结果可以看出,研究区北部及西部生态稳定性保持较好,生态脆弱等级下降主要发生在研究区的东南部,20年间耕地扩张造成的草地被侵占及水资源过度开发利用是造成生态退化的主要原因。评价体系紧紧围绕干旱牧区"水-草-畜"关系设置评价指标,将观测数据与水文模型模拟相结合,统计数据与空间地类相联系,点面结合,能够较好的反映出研究区20年间生态脆弱程度变化趋势及主要问题。     

西方高温热浪研究述评

高温热浪不仅给自然生态系统造成不可逆转的冲击,也对人类经济社会与健康产生巨大的负面影响。西方学者历来重视高温热浪相关研究,并产生了丰硕的成果。首先梳理了西方高温热浪研究的脉络,即从指标测度上明析其内涵;从对比模拟角度分析其产生的机理;从空间格局上总结其分布与规律;从复合系统层面探讨其影响;从流行病学角度解析其对健康的危害。其次,指出了趋势判断、机理解析、影响评估、脆弱性评估、风险感知、适应分析等未来关注的矛盾与焦点。在此基础上,提出了西方高温热浪研究对中国的启示,包括:拓展典型区域的实证研究;关注脆弱群体与欠发达地区;重视健康尤其是心理健康的影响;注重研究领域拓展;尝试多学科视角融合等。     

Spirocyclic dimer SpiD7 activates the unfolded protein response to selectively inhibit growth and induce apoptosis of cancer cells

The unfolded protein response (UPR) is an adaptation mechanism activated to resolve transient accumulation of unfolded/misfolded proteins in the endoplasmic reticulum. Failure to resolve the transient accumulation of such proteins results in UPR-mediated programmed cell death. Loss of tumor suppressor gene or oncogene addiction in cancer cells can result in sustained higher basal UPR levels; however, it is not clear if these higher basal UPR levels in cancer cells can be exploited as a therapeutic strategy. We hypothesized that covalent modification of surface-exposed cysteine (SEC) residues could simulate unfolded/misfolded proteins to activate the UPR, and that higher basal UPR levels in cancer cells would provide the necessary therapeutic window. To test this hypothesis, here we synthesized analogs that can covalently modify multiple SEC residues and evaluated them as UPR activators. We identified a spirocyclic dimer, SpiD7, and evaluated its effects on UPR activation signals, that is, XBP1 splicing, phosphorylation of eIF2α, and a decrease in ATF 6 levels, in normal and cancer cells, which were further confirmed by RNA-Seq analyses. We found that SpiD7 selectively induced caspase-mediated apoptosis in cancer cells, whereas normal cells exhibited robust XBP1 splicing, indicating adaptation to stress. Furthermore, SpiD7 inhibited the growth of high-grade serous carcinoma cell lines ~3-15-fold more potently than immortalized fallopian tube epithelial (paired normal control) cells and reduced clonogenic growth of high-grade serous carcinoma cell lines. Our results suggest that induction of the UPR by covalent modification of SEC residues represents a cancer cell vulnerability and can be exploited to discover novel therapeutics.     

两种HSV-1及猴B病毒相关抗体测定方法的比较

目的以人单纯疱疹病毒（HSV-1）做为抗原,利用空斑法和IFA法比较猴BV和人HSV-1阳性血清两种不同血清的中和能力的差异,建立一种实用、准确、可靠的病毒毒力的检测方法。方法首先,将HSV-1病毒悬液作连续的10倍稀释,取1 mL接种于已经长成单层的Vero-E6细胞上,用1%甲基纤维素覆盖,待其出现蚀斑后计数,算出病毒悬液中每毫升所含蚀斑单位,即滴定出HSV-1的TC ID50。同时,用免疫荧光方法（IFA）对猴和人疱疹阳性血清进行滴定,得到其血清的效价。其次,用滴定出的病毒液分别与两种阳性血清体外中和后,接种到单层的Vero-E6细胞上,用1%甲基纤维素覆盖,待其出现蚀斑后计数。最后,计算出其蚀斑减少率。结果用1%甲基纤维素作覆盖层的蚀斑数量平均为10-5PFU,能形成115-116个/mL蚀斑,形状呈黍米大小的规则圆形,其蚀斑边缘清晰。IFA滴定的人HSV-1阳性血清与猴BV阳性血清的中和抗体均为1∶80。人HSV-1和猴BV两种阳性血清的空斑减少率均为100%。结论确定了利用1%甲基纤维素做为覆盖层可得到清晰可靠的蚀斑,由此方法检测到用人HSV-1可以代替猴B病毒,筛查猴B病毒抗体。且为将来进行药物筛选和中和实验中利用病毒空斑法建立方便、可靠的方法。     

Inter-species variation of photosynthetic and xylem hydraulic traits in the deciduous and evergreen Euphorbiaceae tree species from a seasonally tropical forest in south-western China

The objective of the present study was to examine the functional coordination among hydraulic traits, xylem characteristics and gas exchange rates across three deciduous Euphorbiaceae tree species (Hevea brasiliensis, Macaranga denticulata and Bischofia javanica) and three evergreen Euphorbiaceae tree species (Drypetes indica, Aleurites moluccana and Codiaeum variegatum) from a seasonally tropical forest in south-western China. The deciduous tree species were more vulnerable to water stress-induced embolism than the evergreen tree species. However, the deciduous tree species generally had higher maximal rates of sapwood and leaf-specific hydraulic conductivity (K _S and K _L), respectively. Compared with the evergreen tree species, the deciduous tree species, however, possessed a lower density of sapwood and a wider diameter of xylem vessels. Regardless of leaf phenology, the hydraulic vulnerability and conductivity were significantly correlated with sapwood density and mean vessel diameter. Furthermore, the hydraulic vulnerability was positively correlated with water transport efficiency. In addition, the deciduous tree species exhibited higher maximal photosynthetic rates (A _max) and stomatal conductance (g _max), but lower water use efficiency (WUE). Interestingly, the A _max, g _max and WUE were strongly correlated with K _S and K _L across the deciduous and evergreen tree species. These results suggest that xylem structure, rather than leaf phenology, accounts for the difference in hydraulic traits between the deciduous tree species and the evergreen tree species. Meanwhile, our results show that there is a significant trade-off between hydraulic efficiency and safety, and a strong functional correlation between the hydraulic capacity and gas exchange rates across the deciduous and evergreen tree species.     

用蚀斑法进行麻疹疫苗的病毒滴定

按世界银行中国疫苗项目对麻疹疫苗检定的要求,建立了麻疹疫苗病毒滴定的蚀斑方法。以蚀斑法对Ｈｕ１９１株生产的冻干麻疹活疫苗样品进行了测定。结果发现,Ｈｕ１９１株生产的麻疹疫苗在琼脂和甲基纤维素覆盖下所形成的蚀斑大小及形状不同,但滴度无显著性差异;在室温和３７℃吸附的滴度无显著性差异,但细胞在３７℃生长较好;在本实验条件下,蚀斑滴定和ＣＣＩＤ５０测定的滴度呈正相关,蚀斑滴定相对较敏感;采用国产６孔聚苯乙稀培养板,Ｖｅｒｏ细胞单层,在３７℃吸附,用甲基纤维素覆盖,经结晶紫及甲醛固定染色后计数空斑,操作简便易行,结果特异敏感,重复性好,可用于麻疹疫苗病毒滴定     

环渤海五省市生态风险评价

生态风险评价是近20年来兴起的一个研究热点,是地理学、生态学、环境风险评价等领域的综合交叉点。对生态风险进行研究,有助于了解研究区内的生态环境状况,从而降低生态风险,改善人地关系。环渤海地区是人地矛盾非常突出的典型区域。本文参考美国环保署(USEPA)的框架,根据相对风险评价模型的分级排序思想,评估环渤海五省市的单项及综合生态风险值。采用生态资产表征生态终点,进行风险受体的损失度量;通过构建评价指标体系,采用主成分分析和ArcGIS加权叠加的方法,进行受体环境的脆弱性评价;基于土地利用和植被斑块数据,利用景观格局指数法评价生态系统的结构易损性。结果表明:海陆交错带、山地丘陵区和城市是典型的高生态风险区域;低风险区域主要分布在地形、水热、植被状况好、灾害频次低的平原和部分丘陵地区。对于高风险的区域,做好风险防范至关重要。     

干旱区内陆河流域农户生计对生态退化的脆弱性评价——以石羊河中下游为例

生态退化对干旱区内陆河流域农业人口的负面影响非常显著,当前急需评估农户生计对生态退化的脆弱性,识别脆弱群体及脆弱性成因,并依此寻求降低生计脆弱性的对策措施。以石羊河中下游为研究区,基于366户农户调查数据,分析了不同类型农户对生态退化的暴露度、敏感性及适应能力,评估了农户生计对生态退化的脆弱性,探明了影响农户生计对生态退化脆弱性的关键因素。结果表明:(1)高收入及高文化程度农户的适应能力强,暴露度与敏感性高,生计脆弱性较低;(2)从纯农户到非农户、从单一生计农户到多种生计农户,适应能力依次增强,暴露度与敏感性递减,生计脆弱性降低;(3)改善生态环境质量,提高农户的富裕水平、受教育程度及社会资本,促进生计转型能够显著降低农户生计对生态退化的脆弱性。最后,提出了减轻农户生计脆弱性的对策建议及未来需进一步关注的问题。     

文山松毛虫质型多角体病毒在Sf21细胞中离体增殖试验

研究了文山松毛虫质型多角体病毒(DpCPV-W)在Sf21细胞中的离体增殖行为,并进行了空斑试验,结果显示DpCPV-W毒株能够在Sf21细胞中增殖,也能在Sf21细胞上形成空斑,并能产生形态正常的病毒多角体.在DpCPV-W中加入基因工程增效蛋白,能显著增加病毒粒子对离体细胞的感染率,增幅达145%.生物测定表明,离体增殖的病毒多角体与虫体增殖的多角体的毒力相当.因此,Sf21细胞可以作为文山松毛虫CPV增殖行为研究的离体细胞系统,也可通过空斑纯化技术对文山松毛虫CPV生产防治的病毒种进行单个病毒粒子的分离纯化.