Vegetation and plant food reconstruction of lowermost Bed II, Olduvai Gorge, using modern analogs

Vegetation and plant foods for hominins of lowermost Bed II, Olduvai Gorge were modeled by examining vegetation in modern habitats in northern Tanzania (Lake Manyara, Ngorongoro, Serengeti) that are analogous to the paleolandscape in terms of climate, land forms, and soil types, as indicated by previous paleoenvironmental studies of Olduvai. Plant species in the modern habitats were identified in a series of sample plots, and those known to be eaten by modern humans, chimpanzees, or baboons were considered potentially edible for early hominins. Within the 50-80 kyr deposition of lowermost Bed II, periods of drier climate were characterized by low lake stands and a broad eastern lacustrine plain containing a mosaic of springs, marsh, woodland, and edaphic grassland. Based on results of this study, plant food diversity in each of those habitats was relatively low, but the mosaic nature of the area meant that hominins could reach several different habitat types within short distances, with access to potential plant foods including marsh plants, grass grains, roots, shrub fruits, edible parts from palms, leafy herbaceous plants, and Acacia pods, flowers, and gum. Based on Manyara analogs, a greater variety of plant foods, such as tree fruits (e.g., Ficus, Trichilia) and the roots and fruits of shrubs (e.g., Cordia, Salvadora) would be expected further east along the rivers in the lacustrine terrace and alluvial fans. Interfluves of the alluvial fans were probably less wooded and offered relatively fewer varieties of plant foods, but there is sparse paleoenvironmental evidence for the character of Olduvai's alluvial fans, making the choice of appropriate modern analogs difficult. In the western side of the basin, based on modern analogs in the Serengeti, riverine habitats provided the greatest variety of edible plant food species (e.g., Acacia, Grewia, Justicia). If the interfluves were grassland, then a large variety of potentially edible grasses and forbs were present seasonally. Periods of wetter climate resulted in a much expanded paleolake and a shrinking of the eastern lacustrine plain mosaic into a narrow zone. The dominant landscape features were then forest-lined rivers in the eastern alluvial fans, and rivers in the western side of the paleo-Olduvai basin were also better watered at these times, supporting denser woody vegetation with large varieties of edible fruits, leaves, and underground parts.     

Doubly robust estimates for binary longitudinal data analysis with missing response and missing covariates

Longitudinal studies often feature incomplete response and covariate data. Likelihood-based methods such as the expectation-maximization algorithm give consistent estimators for model parameters when data are missing at random (MAR) provided that the response model and the missing covariate model are correctly specified; however, we do not need to specify the missing data mechanism. An alternative method is the weighted estimating equation, which gives consistent estimators if the missing data and response models are correctly specified; however, we do not need to specify the distribution of the covariates that have missing values. In this article, we develop a doubly robust estimation method for longitudinal data with missing response and missing covariate when data are MAR. This method is appealing in that it can provide consistent estimators if either the missing data model or the missing covariate model is correctly specified. Simulation studies demonstrate that this method performs well in a variety of situations.     

Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells

Vacuoles in plant cells can be eliminated by centrifugation of protoplasts through a density gradient. In this review, properties of evacuolated protoplasts, named ‘miniprotoplasts’, and the significant roles in plant cytoskeleton studies are described. Miniprotoplasts, prepared from tobacco BY-2 cells whose cell-cycle had been synchronized at late anaphase, continued to divide to form two daughter cells. In the presence of cytochalasin B cytokinetic cleavage was enhanced, suggesting a role of actin filaments in plant cytokinesis. In the cytoplasmic extract of miniprotoplasts both tubulin and actin could be polymerized to form microtubules (MTs) and actin filaments (AFs), respectively. A purification method for tubulin, actin and related proteins was developed using the extract. To investigate the interaction between cortical microtubules and the plasma membrane, an experimental system in which MTs were reconstructed on membrane ghosts was developed by combination of membrane ghosts and the extract.     

硫化氢在心血管保护过程中免疫炎症调节研究进展

硫化氢(hydrogen sulfide,H_2S)是一种无色、具有臭鸡蛋气味的气体,过去认为只是一种有毒的气体。近年来大量研究证实H_2S是继一氧化氮(nitric oxide,NO)和一氧化碳(carbon oxide,CO)后第三种内源性气体信号分子,同时,H_2S在心血管系统疾病发生、发展过程中起关键的调控作用,但其机制还不明确,已有报道主要通过抗凋亡、抗氧化、调节内皮一氧化氮合酶活性、促进血管新生等;而本文总结了H_2S在缺血性心脏病、动脉粥样硬化等心血管疾病中免疫炎症调节作用及其机制,从而为H_2S生物学功能以及相关心血管疾病的防治提供新的思路。     

Intrinsic pKas of ionizable residues in proteins: An explicit solvent calculation for lysozyme

Molecular dynamics simulations of triclinic hen egg white lysozyme in aqueous solution were performed to calculate the intrinsic pK_as of 14 ionizable residues. An all-atom model was used for both solvent and solute, and a single 180 ps simulation in conjunction with a Gaussian fluctuation analysis method was used. An advantage of the Gaussian fluctuation method is that it only requires a single simulation of the system in a reference state to calculate all the pK_as in the protein, in contrast to multiple simulations for the free energy perturbation method. pK_int shifts with respect to reference titratable residues were evaluated and compared to results obtained using a finite difference Poisson-Boltzmann (FDPB) method with a continuum solvent model; overall agreement with the direction of the shifts was generally observed, though the magnitude of the shifts was typically larger with the explicit solvent model. The contribution of the first solvation shell to the total charging free energies of the titratable groups was explicitly evaluated and found to be significant. Dielectric shielding between pairs of titratable groups was examined and found to be smaller than expected. The effect of the approximations used to treat the long-range interactions on the pK_int shifts is discussed. © 1994 Wiley-Liss, Inc.     

植物种质离体保存技术研究进展

本文综述了国内外植物种质离体保存技术最新研究进展。科学家们已发展了一系列行之有效的离体保存技术体系,进一步建立长期、安全、适用的离体保存技术体系及探索保存过程中遗传变异情况是今后的重要研究方向