猪瘟病毒p80基因NTPase/RNA解旋酶功能区在E.coli中的表达

采用PCR技术扩增出猪瘟病毒GXYL株和C株p80基因的NTPase／RNA解旋酶功能区(sGXNS3和sCNS3),将其克隆到表达载体pET-30a( )中,获得重组质粒pET—sGXNS3和pET～sCNS3。PCR、酶切和序列分析鉴定目的基因插入位置、方向和读码框完全正确。1．0mmol／L IPTG诱导得到分子量为26kD的目的蛋白。Western blot检测表明,表达的目的蛋白能被CSFV阳性血清识别。     

Leptin

It is well established that the adipocyte-derived hormone leptin is an important circulating satiety factor that regulates body weight and food intake via its actions on specific hypothalamic nuclei. However, there is growing evidence that leptin and its receptors are widely expressed throughout the brain, in regions not generally associated with energy homeostasis, such as cortex, cerebellum, brainstem, basal ganglia, and hippocampus. In this review the author discusses recent advances made in leptin neurobiology, with particular emphasis on the role of this endocrine peptide in normal and pathophysiological hippocampal function.     

无突叶蝉属二新种 (同翅目：叶蝉科，离脉叶蝉亚科)

 记述了无突叶蝉属Taharana二新种：指无突叶蝉 T. digitata sp.nov.和尖板无突叶蝉T. cuspidata sp.nov.。模式标本存放于 安徽农业大学植保系昆虫分类研究室     

Les gisements de Galería,Gran Dolina TD10 et Ambrona (le Complexe Inférieur) : trois modèles technologiques dans le deuxième tiers du Pléistocène moyen

In Europe, the second third of the middle Pleistocene is a key period in which the human settlement is increased, the occupied habitat multiplies, the use of fire begins, the Mode 2 generalizes, Mode 3 begins and the method of exploitation levallois develops. At this moment the occupations of Galería, Gran Dolina and Ambrona are developing. These three deposits of the north Plateau have a very complete archaeological record and a very long development in time, thus they represent three technological different models. These sites represent three technological different models that are determined by the quality of the raw materials that were used, the control and the safety of the sites, how many time it was spend in the knapping, the characteristics of the edges, they functionality and the cultural traditions.     

La Grotte des Félins, site paléolithique du Pléistocène supérieur à Dar Bouazza (Maroc)

In the continental sediments filling a cave that developed in Middle Pleistocene deposits near Casablanca, Morocco, a very rich mammal fauna from the last climatic cycle was discovered. It was associated with a Middle Palaeolithic poor and atypical lithic assemblage manufactured on blocks and pebbles. The lower fossiliferous layer contained abundant carbonized remains of felids. Unfortunately, the site was destroyed by quarrying before adequate excavations could be carried out.     

Candoninae trapézoïdales (Crustacea, Ostracoda) du Bassin de Turiec (Slovaquie) du Miocène supérieur : systématique, écologie et évolution

The trapezoidal Candoninae from the Turiec Basin in Slovakia referred to the Candona sitari- and to C. aculeata-groups have characters that contrast with the valves of the Recent central European Candonines. The particularities concern the degree of calcification, the zone of concrescence, the overlap, and the presence of protuberances, the dorsum and the tubercles. Within the sitari-group, these characters evolve simultaneously with rapid evolution of the valve outline from subtrapezoidal to trapezoidal in shape. The species of this group acquire valve contours similar to those of the trapezoidal Candoninae of the Lake Pannon and the recent fauna of the long-lived lakes. The trapezoidal Candoninae of the Turiec Basin are associated with candonines that have triangular and rectangular carapaces with a pointed posteroventral margin. These three morphotypes indicate relatively stable habitats with weak environmental fluctuations. The trapezoidal species are rare in the littoral facies in both the north and the marginal areas of the Turiec Basin, but very abundant in the centre and south of the Basin. They probably lived in a profundal environment and their evolution probably occurred in relation to both sexual reproduction and a benthonic mode of life. The two groups comprise 18 species, of which 15 are described as new.     

Eurocharax Tourainei nov. gen., nov. sp. (poisson teleosteen, Ostariophysi): Nouveau Characidae fossile des «Calcaires a Bythinies du Var

The study of fossil Fishes preserved in the «calcaires à Bythinies of Montmeyan, la Mourotte and Saint-Julien-le-Montagné basins (Var. France), makes possible the demonstration of their referal to the family Characidae: These Fishes, here mentioned as Eurocharax tourainei nov. gen., nov. sp., clearly differ from the Lower Eocene Characid fishes of Languedoc and Paris Basin by their set of teeth. The brackish condition of the “calcaires à Bythinies is taken into account for explaining the probable biogeographical history of these Fishes.     

Presence of Hemitragus aff. cedrensis (Mammalia, Bovidae) in the Iberian Peninsula: Biochronological and biogeographical implications of its discovery at Bolomor Cave (Valencia, Spain)

The discovery of new material in Late Pleistocene levels at Bolomor Cave (Valencia, Spain) raises some questions about the presence of the most ancient record of Hemitragus cedrensis in the peninsula, and its dispersal out of Provence. The morphology and dimensions of some lower teeth confirm the identification of H. aff. cedrensis. Moreover, it presents strong similarities, both morphological and metrical, with the specimens from Caune de l’Arago and bau de l’Aubesier (end of OIS 7 to OIS 5e) rather than with the population from the eponymous locality. The data suggest a dispersal event out of Provence towards the Iberian Peninsula during the Eemian. This dispersal was not stopped by natural barriers such as large rivers, or mountains. The results presented here confirm the biochronological interest of the genus Hemitragus for the Late Pleistocene in Mediterranean Europe.     

Les matières premières,la technologie lithique et les stratégies d’occupation dans le site du Pléistocène moyen de Covacha de los Zarpazos (gisement de Galería,Sierra de Atapuerca,Espagne)

Variability is one of the most debated topics in lithic technology studies. For the Middle Pleistocene period, this debate has specifically focused on the significance of large cutting tools such as handaxes and cleavers. This paper presents the stone tool assemblage from Covacha de los Zarpazos site, included in the Galería complex at Sierra de Atapuerca (Burgos, Spain). We analyse the management of lithic raw materials, the reduction sequences identified, and the morphological variation in the shape of large cutting tools. The results show that raw materials play an important role in the final variability of tool shape, although they do not represent any real constraint. However, what determines the main variability of a whole lithic assemblage is the occupation strategy and the specific activities carried out in a given site.     

Biostratigraphie des conodontesdu Muschelkalk supérieur luxembourgeois

Biostratigraphic scale of conodonts established inthe germanic basin is found in Luxemburg. The repartition of associations allows to determine six zones which can be compared to those of neighbouring areas.