全文获取类型
收费全文 | 4172篇 |
免费 | 406篇 |
国内免费 | 360篇 |
专业分类
4938篇 |
出版年
2024年 | 12篇 |
2023年 | 96篇 |
2022年 | 63篇 |
2021年 | 143篇 |
2020年 | 171篇 |
2019年 | 174篇 |
2018年 | 128篇 |
2017年 | 122篇 |
2016年 | 143篇 |
2015年 | 176篇 |
2014年 | 170篇 |
2013年 | 262篇 |
2012年 | 174篇 |
2011年 | 184篇 |
2010年 | 179篇 |
2009年 | 179篇 |
2008年 | 203篇 |
2007年 | 206篇 |
2006年 | 187篇 |
2005年 | 174篇 |
2004年 | 147篇 |
2003年 | 164篇 |
2002年 | 171篇 |
2001年 | 154篇 |
2000年 | 100篇 |
1999年 | 110篇 |
1998年 | 89篇 |
1997年 | 97篇 |
1996年 | 90篇 |
1995年 | 75篇 |
1994年 | 77篇 |
1993年 | 54篇 |
1992年 | 58篇 |
1991年 | 51篇 |
1990年 | 18篇 |
1989年 | 30篇 |
1988年 | 26篇 |
1987年 | 24篇 |
1986年 | 17篇 |
1985年 | 39篇 |
1984年 | 35篇 |
1983年 | 30篇 |
1982年 | 30篇 |
1981年 | 18篇 |
1980年 | 21篇 |
1979年 | 15篇 |
1978年 | 11篇 |
1977年 | 7篇 |
1976年 | 10篇 |
1973年 | 10篇 |
排序方式: 共有4938条查询结果,搜索用时 9 毫秒
951.
952.
953.
Early burst in body size evolution is uncoupled from species diversification in diving beetles (Dytiscidae) 下载免费PDF全文
Aurélie Désamoré Benjamin Laenen Kelly B. Miller Johannes Bergsten 《Molecular ecology》2018,27(4):979-993
Changes in morphology are often thought to be linked to changes in species diversification, which is expected to leave a signal of early burst (EB) in phenotypic traits. However, such signal is rarely recovered in empirical phylogenies, even for groups with well‐known adaptive radiation. Using a comprehensive phylogenetic approach in Dytiscidae, which harbours ~4,300 species with as much as 50‐fold variation in body size among them, we ask whether pattern of species diversification correlates with morphological evolution. Additionally, we test whether the large variation in body size is linked to habitat preference and whether the latter influences species turnover. We found, in sharp contrast to most animal groups, that Dytiscidae body size evolution follows an early‐burst model with subsequent high phylogenetic conservatism. However, we found no evidence for associated shifts in species diversification, which point to an uncoupled evolution of morphology and species diversification. We recovered the ancestral habitat of Dytiscidae as lentic (standing water), with many transitions to lotic habitat (running water) that are concomitant to a decrease in body size. Finally, we found no evidence for difference in net diversification rates between habitats nor difference in turnover in lentic and lotic species. This result, together with recent findings in dragonflies, contrasts with some theoretical expectations of the habitat stability hypothesis. Thus, a thorough reassessment of the impact of dispersal, gene flow and range size on the speciation process is needed to fully encompass the evolutionary consequences of the lentic–lotic divide for freshwater fauna. 相似文献
954.
Takahiro Ohde Yusuke Takehana Takahiro Shiotsuki Teruyuki Niimi 《Arthropod Structure & Development》2018,47(4):362-369
Despite previous developmental studies on basally branching wingless insects and crustaceans, the evolutionary origin of insect wings remains controversial. Knowledge regarding genetic regulation of tissues hypothesized to have given rise to wings would help to elucidate how ancestral development changed to allow the evolution of true wings. However, genetic tools available for basally branching wingless species are limited. The firebrat Thermobia domestica is an apterygote species, phylogenetically related to winged insects. T. domestica presents a suitable morphology to investigate the origin of wings, as it forms the tergal paranotum, from which wings are hypothesized to have originated. Here we report the first successful CRISPR/Cas9-based germline genome editing in T. domestica. We provide a technological platform to understand the development of tissues hypothesized to have given rise to wings in an insect with a pre-wing evolution body plan. 相似文献
955.
Hilaria E. Amuzu Kirill Tsyganov Cassandra Koh Rosemarie I. Herbert David R. Powell Elizabeth A. McGraw 《Ecology and evolution》2018,8(11):5441-5454
Mosquitoes transmit a diverse group of human flaviviruses including West Nile, dengue, yellow fever, and Zika viruses. Mosquitoes are also naturally infected with insect‐specific flaviviruses (ISFs), a subgroup of the family not capable of infecting vertebrates. Although ISFs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. Wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits the replication of co‐infecting pathogens, including flaviviruses. Artificially created Wolbachia‐infected Aedes aegypti mosquitoes are being released into the wild in a series of trials around the globe with the hope of interrupting dengue and Zika virus transmission from mosquitoes to humans. Our work investigated the effect of Wolbachia on ISF infection in wild‐caught Ae. aegypti mosquitoes from field release zones. All field mosquitoes were screened for the presence of ISFs using general degenerate flavivirus primers and their PCR amplicons sequenced. ISFs were found to be common and widely distributed in Ae. aegypti populations. Field mosquitoes consistently had higher ISF infection rates and viral loads compared to laboratory colony material indicating that environmental conditions may modulate ISF infection in Ae. aegypti. Surprisingly, higher ISF infection rates and loads were found in Wolbachia‐infected mosquitoes compared to the Wolbachia‐free mosquitoes. Our findings demonstrate that the symbiont is capable of manipulating the mosquito virome and that Wolbachia‐mediated viral inhibition is not universal for flaviviruses. This may have implications for the Wolbachia‐based DENV control strategy if ISFs confer fitness effects or alter mosquito susceptibility to other flaviviruses. 相似文献
956.
Pollinator‐mediated selection on floral size and tube color in Linum pubescens: Can differential behavior and preference in different times of the day maintain dimorphism? 下载免费PDF全文
Diversity of flower traits is often proposed as the outcome of selection exerted by pollinators. Positive directional pollinator‐mediated selection on floral size has been widely shown to reduce phenotypic variance. However, the underlying mechanism of maintaining within‐population floral color polymorphism is poorly understood. Divergent selection, mediated by different pollinators or by both mutualists and antagonists, may create and maintain such polymorphism, but it has rarely been shown to result from differential behavior of one pollinator. We tested whether different behaviors of the same pollinators in morning and evening are associated with dimorphic floral trait in Linum pubescens, a Mediterranean annual plant that exhibits variable within‐population frequencies of dark‐ and light‐colored flower tubes. Usia bicolor bee‐flies, the major pollinators of L. pubescens, are mostly feeding in the flower in the morning, while in the evening they are mostly visiting the flowers for mating. In 2 years of studying L. pubescens in a single large population in the Carmel, Israel, we found in one year that dark‐centered flowers received significantly higher fraction of visits in the morning. Fitness was positively affected by number of visits, but no fitness differences were found between tube‐color morphs, suggesting that both morphs have similar pollination success. Using mediation analysis, we found that flower size was under positive directional pollinator‐mediated selection in both years, but pollinator behavior did not explain entirely this selection, which was possibly mediated also by other agents, such as florivores or a‐biotic stresses. While most pollinator‐mediated selection studies show that flower size signals food reward, in L. pubescens, it may also signal for mating place, which may drive positive selection. While flower size found to be under pollinator‐mediated selection in L. pubescens, differential behavior of the pollinators in morning and evening did not seem to explain flower color polymorphism. 相似文献
957.
Incorporating variability in simulations of seasonally forced phenology using integral projection models 下载免费PDF全文
Devin W. Goodsman Brian H. Aukema Nate G. McDowell Richard S. Middleton Chonggang Xu 《Ecology and evolution》2018,8(1):162-175
Phenology models are becoming increasingly important tools to accurately predict how climate change will impact the life histories of organisms. We propose a class of integral projection phenology models derived from stochastic individual‐based models of insect development and demography. Our derivation, which is based on the rate summation concept, produces integral projection models that capture the effect of phenotypic rate variability on insect phenology, but which are typically more computationally frugal than equivalent individual‐based phenology models. We demonstrate our approach using a temperature‐dependent model of the demography of the mountain pine beetle (Dendroctonus ponderosae Hopkins), an insect that kills mature pine trees. This work illustrates how a wide range of stochastic phenology models can be reformulated as integral projection models. Due to their computational efficiency, these integral projection models are suitable for deployment in large‐scale simulations, such as studies of altered pest distributions under climate change. 相似文献
958.
Fengchun Ye Ulrich Melcher John E. Rascoe Jacqueline Fletcher 《Biochemical genetics》1996,34(7-8):269-286
Genetic variations in the plant pathogen,Spiroplasma citri strain BR3, were characterized through physical genome mapping of the original isolate, BR3-3X, and two derivatives, BR3-T
and BR3-G, obtained after several years of different maintenance conditions. BR3-T was transmitted from plant to plant via
its natural insect vector, the leafhopperCirculifer tenellus, while BR3-G was maintained only in plants by periodic grafting and has lost its ability to be insect transmitted. By pulsed
field gel electrophoresis (PFGE) analysis and DNA hybridization, extensive changes in chromosomal DNA restriction patterns
relative to the parent, BR3-3X, were observed in both BR3-T and BR3-G, each of which also had a larger genome size than the
parent line. Genetic organization was relatively conserved between BR3-T and BR3-3X. In contrast, a large chromosomal inversion
and deletions of approximately 10 kb near each of the inversion borders were observed in BR3-G. One of the deletions, which
included several possibly functional genes, was closely linked to a SpV1-related transposase gene. The locations of the deletion
borders were also determined. The results of this study demonstrated remarkable genome instability of spiroplasmas. 相似文献
959.
Robert L. Mangan 《Entomologia Experimentalis et Applicata》1996,79(2):153-159
Mexican fruit flies, Anastrepha ludens (Loew), that are reared, sterilized and released for maintenance of a fly free zone in south Texas and for population eradication in California are routinely chilled for 1 to 2 h to facilitate handling and dispersal. While a number of behavioral, physical and physiological characteristics are routinely monitored as part of a quality control program, the effect of the chilling process on the ability of these males to attract wild females has not been evaluated. Tests were performed to compare the mate attracting effectiveness of mass reared, sterilized, chilled males with sterile males that had not been chilled and with recently colonized males. Tests were performed with two recently colonized strains of females in a large cage and males of the three types in plastic cups set on top of the cage. Controls consisted of empty cups. Results showed no significant differences among the three types of males. In tests with the most recently collected (least laboratory adapted) wild strain of females, the chilled males attracted the most females. Fly age and time of day were both significantly related to total attractiveness of males. Number of females responding to males per day increased over the five-day test period. The time period when female response was greatest was variable among the tests. 相似文献
960.
The design features of gustatory systems are considered in relation to the need for insect herbivores (or indeed any animal) to regulate their nutrient intake. The gustatory system (defined to include both peripheral and central components) should ideally be designed to reflect: (a) the average nutritional requirements of the insect during its various developmental stages; (b) the predictably unpredictable variation in nutrient supply-demand which occurs about that average; and (c) the distribution and composition of foods available in the environment. A mathematical model is presented which makes detailed predictions regarding behavioural and electrophysiological responses to foods or simple solutions varying in nutrient content. 相似文献