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41.
Tjakko Abee Jan Knol Klaas J. Hellingwerf Evert P. Bakker Annette Siebers Wil N. Konings 《Archives of microbiology》1992,158(5):374-380
Cells of the purple non-sulphur bacterium Rhodobacter sphaeroides express a high-affinity K+ uptake system when grown in media with low K+ concentrations. Antibodies againts the catalytic KdpB protein or the whole KdpABC complex of Escherichia coli crossreact with a 70.0 kDa R. sphaeroides protein that was expressed only in cells grown in media with low K+ concentrations. In membranes derived from R. sphaeroides cells grown with low K+ concentrations (induced cells), a high ATPase activity could be detected when assayed in Tris-HCl pH 8.0 containing 1 mM MgSO4. This ATPase activity increased upon addition of 1 mM KCl from 166 to 289 mol ATP hydrolysed x min-1 x g protein-1 (1.7-fold stimulation). The K+-stimulated ATPase activity was inhibited approximately 93% by 0.5 mM vanadate but hardly by N,N-dicyclohexylcarbo-diimide (DCCD). These results indicate that the inducible K+-ATPase in R. sphaeroides resembles the Kdp K+-translocating ATPase of Escherichia coli. This Kdp-like transport system is also expressed in R. capsulatus and Rhodospirillum rubrum during growth in media with low K+ concentrations suggesting a wide distribution of this transport system among phototrophic bacteria.Abbreviations
electrical potential difference across the cytoplasmic membrane
- pH
pH difference across the cytoplasmic membrane
- BSA
bovine serum albumine
- PAGE
polyacrylamide gel electrophoresis
- HEPES
4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid
- PMSF
phenyl-methyl-sulfonyl fluoride
- DCCD
N,N-dicyclohexylcarbodiimide
- AIB
2--aminoisobutyric acid
- TMG
methyl--d-thiogalactopyranoside 相似文献
42.
Photosynthetic capacity and leaf properties of sun and shade leaves of overstorey sweetgum trees (Liquidambar styraciflua L.) were compared over the first 3 years of growth in ambient or ambient + 200 μL L?1 CO2 at the Duke Forest Free Air CO2 Enrichment (FACE) experiment. We were interested in whether photosynthetic down‐regulation to CO2 occurred in sweetgum trees growing in a forest ecosystem, whether shade leaves down‐regulated to a greater extent than sun leaves, and if there was a seasonal component to photosynthetic down‐regulation. During June and September of each year, we measured net photosynthesis (A) versus the calculated intercellular CO2 concentration (Ci) in situ and analysed these response curves using a biochemical model that described the limitations imposed by the amount and activity of ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Vcmax) and by the rate of ribulose‐1,5‐bisphosphate (RuBP) regeneration mediated by electron transport (Jmax). There was no evidence of photosynthetic down‐regulation to CO2 in either sun or shade leaves of sweetgum trees over the 3 years of measurements. Elevated CO2 did not significantly affect Vcmax or Jmax. The ratio of Vcmax to Jmax was relatively constant, averaging 2·12, and was not affected by CO2 treatment, position in the canopy, or measurement period. Furthermore, CO2 enrichment did not affect leaf nitrogen per unit leaf area (Na), chlorophyll or total non‐structural carbohydrates of sun or shade leaves. We did, however, find a strong relationship between Na and the modelled components of photosynthetic capacity, Vcmax and Jmax. Our data over the first 3 years of this experiment corroborate observations that trees rooted in the ground may not exhibit symptoms of photosynthetic down‐regulation as quickly as tree seedlings growing in pots. There was a strong sustained enhancement of photosynthesis by CO2 enrichment whereby light‐saturated net photosynthesis of sun leaves was stimulated by 63% and light‐saturated net photosynthesis of shade leaves was stimulated by 48% when averaged over the 3 years. This study suggests that this CO2 enhancement of photosynthesis will be sustained in the Duke Forest FACE experiment as long as soil N availability keeps pace with photosynthetic and growth processes. 相似文献
43.
Muhammad Akbar Anjum 《Acta Physiologiae Plantarum》2008,30(1):43-52
Six-months-old, uniform sized seedlings of two citrus rootstocks; Cleopatra mandarin (Citrus reshni Hort. ex Tan) and Troyer citrange (Poncirus trifoliata × Citrus sinensis) were irrigated with half-strength Hoagland nutrient solution containing 0, 40 or 80 mM NaCl for 12 weeks. Shoot height,
leaf number and fresh weights of the seedlings, and relative chlorophyll contents, chlorophyll fluorescence yields (Fv/Fm),
net photosynthetic and respiration rates in the leaves decreased with the increase in salinity level in the irrigation water.
The decrease was greater in Troyer citrange as compared to Cleopatra mandarin. The concentrations of sugars i.e. fructose,
glucose and sucrose in the leaves of Cleopatra mandarin and both leaves and roots of Troyer citrange decreased with the increase
in salinity level. However, the concentrations in the roots of Cleopatra mandarin increased with the increase in salinity
level. Free proline content in the leaves of Troyer citrange and root tissue of Cleopatra mandarin also increased with the
increased salinity level. Among the polyamines, spermine titer increased in the leaves of both rootstocks as a response to
salinity treatments. Na+ concentrations were higher in leaf and root tissue of Cleopatra mandarin, while that of Cl− were higher in Troyer citrange. 相似文献
44.
Y. Kitaya L. Xiao A. Masuda T. Ozawa M. Tsuda K. Omasa 《Journal of applied phycology》2008,20(5):737-742
Symbiotic dinoflagellates of the species Amphidinium are expected to be pharmaceutically useful microalgae because they produce antitumor macrolides. A microalgae production
system with a large number of cells at a high density has been developed for the efficient production of macrolide compounds.
In the present study, the effects of culture conditions on the cellular growth rate of dinoflagellates were investigated to
determine the optimum culture conditions for obtaining high yields of microalgae. Amphidinium species was cultured under conditions with six temperature levels (21–35°C), six levels of photosynthetic photon flux density
(15–70 μmol photons m−2 s−1), three levels of CO2 concentration (0.02–0.1%), and three levels of O2 concentration (0.2–21%). The number of cells cultured in a certain volume of solution was monitored microscopically and the
cellular growth rate was expressed as the specific growth rate. The maximum specific growth rate was 0.022 h−1 at a temperature of 26°C and O2 concentration of 5%, and the specific growth rate was saturated at a CO2 concentration of 0.05%, a photosynthetic photon flux density of 35 μmol photons m−2 s−1 and a photoperiod of 12 h day−1 upon increasing each environmental parameter. The results demonstrate that Amphidinium species can multiply efficiently under conditions of relatively low light intensity and low O2 concentration. 相似文献
45.
Aelita Konoplyova Yiola Petropoulou Charilaos Yiotis George K. Psaras Yiannis Manetas 《Flora》2008,203(8):653-662
The leaves of some plants display an optical patchiness on their upper side, displaying light- and dark-green areas with high and low reflectance, respectively. In this investigation, we studied the fine structure of the corresponding sectors and we asked whether the lost reflected light entails a photosynthetic cost to these leaves. Four species, i.e. Arum italicum, Ranunculus ficaria, Cyclamen hederifolium and Cyclamen persicum were investigated. Scanning electron microscope examination revealed that epidermal cells of light-green sectors of all species are more bulgy than corresponding cells of neighboring dark-green leaf sectors. The comparative anatomical study revealed that (i) epidermis thickness of the light-green areas and the number of mesophyll cell layers does not differ from those of the adjacent dark-green leaf sectors and (ii) palisade cells of light-green sectors are slightly larger and more loosely arranged, allowing a much higher percentage of intercellular air spaces. The latter histological feature seems to provide the structural basis for the different optical properties between the two leaf sectors. Contrary to expectations, net photosynthetic rates (expressed on a leaf area basis) were similar in the light-green and the dark-green areas of the two cyclamen species. Yet, in C. persicum net photosynthesis was higher in the light-green areas, if expressed on a dry mass basis. The small size of the light-green spots in the rest of the test plants precluded CO2 assimilation measurements, yet maximum linear photosynthetic electron transport rates displayed no differences between the two sectors in all plants. Thus, the assumption of a photosynthetic cost in the light-green areas was not confirmed. On the contrary, a higher construction cost was evident in the dark-green areas of three species, displaying a significantly higher specific leaf mass, without any photosynthetic benefit. The results on net photosynthesis were compatible with leaf optical properties and pigment levels. Thus, in spite of the considerably higher reflectance of the light-green areas and their lower (yet normal for a green leaf) chlorophyll levels, corresponding differences in absorptance were slight. In addition, dry mass-based pigment contents in dark-green areas were higher, while chlorophyll a/b (in two species) and carotenoid/chlorophyll ratios (in three species) were lower, pointing to a shade adaptation in these sectors. We conclude that in variegated leaves of this kind, dark-green areas are more costly to build and probably less photosynthetically active. We argue that the high pigment contents of dark-green areas establish steep light gradients in the corresponding mesophyll, rendering deeper chloroplast layers more shade adapted. 相似文献
46.
Gametophyte cells of brown algae Laminaria japonica were employed both in a modified silicone tubular membrane-aerated photobioreactor (bubble-less cultivation mode) and a bubble-column
photobioreactor (bubbling cultivation mode), to study different gas–liquid mixing modes on cell growth rate and cell physiological
status. With an inoculum density of 50 mg DCW l−1, in modified artificial Pacific seawater (APSW) medium at 13°C, light intensity of 60 μE m−2 s−1, light cycle of 16/8 h L/D, and aeration rate of 60 ml min−1, the specific growth rates were 0.082 d−1 for bubble-less mode and 0.070 d−1 for bubbling mode with biomass, in the form of dry cell density, increasing 10.9 and 6.8 times, respectively, during the
36 days’ photolithotrophic cultivation. The specific oxygen evolution rate under bubble-less mode was 39.6% higher than under
bubbling mode on the 18th day. The gametophyte cells grew in cell aggregates with clump sizes, at day 36, of 1.5 mm and 0.5 mm
diameter under bubble-less and bubbling mode respectively and cell injury percentages of 5.1% and 21.1%, respectively. The
silicone tubular membrane-aerated photobioreactor was better suited for the cultivation of fragile macroalgal gametophyte
cells due to the absence of hydrodynamic shear stress caused by fluid turbulence and the presence of a bubble-less gas supply. 相似文献
47.
Antibiotic production by the marine photosynthetic bacterium Chromatium purpuratum NKPB 031704: localization of activity to the chromatophores 总被引:2,自引:0,他引:2
J.Grant Burgess Hideaki Miyashita Hiroaki Sudo Tadashi Matsunaga 《FEMS microbiology letters》1991,84(3):301-306
Abstract Over 200 strains of marine purple photosynthetic bacteria were isolated. Two strains showed antibiotic activity towards Saccharomyces cerevisiae and were tentatively identified as Chromatium purpuratum . Crude antibiotic, prepared by solvent extraction, showed a broad antimicrobial spectrum. The highest activity was found in the chromatophore fraction. Chromatographic separation of purified light harvesting complex from one strain, NKPB 031704, showed the presence of two separate pigmented compounds which were responsible for antimicrobial activity. Our findings reveal the unexpected ability of photosynthetic bacteria to produce broad spectrum antibiotics. In addition, this is the first example of intracellular localization of antibiotic activity in a marine bacterium. 相似文献
48.
In this paper we attempt a functional and spectral characterization of the membrane-bound cytochromes involved in respiratory electron transport by membranes from cells of Chloroflexus aurantiacus grown in the dark under oxygen saturated conditions. We conclude that the NADH-dependent respiration is carried out by a branched respiratory chain leading to two oxidases which differ in sensitivity to CN- and CO. The two routes also show a different sensitivity to the ubiquinone analogue, HQNO, the pathway through the cytochrome c oxidase being fully blocked by 5 M HQNO, whereas the alternative one is insensitive to this inhibitor. The cytochrome c oxidase containing branch is composed by at least two c-type haems with E
m 7.0 of +130 and +270 mV ( bands at 550/553 nm and 549 nm, respectively), plus a b-type cytochrome with E
m 7.0 of +50 mV ( band at 561 nm). From this, and previous work, we conclude that respiratory and photosynthetic electron transport components are assembled together and function on a single undifferentiated plasma membrane.Abbreviations HQNO
heptylhydroxy-quinoline-N-oxide
- UHDBT
undecyl-hydroxydioxobenthiazole
- Q/b-c
ubiquinol/cytochrome c oxidoreductase complex
- BChl
bacteriochlorophyll 相似文献
49.
生长光强对六个橡胶树品种幼苗光合特性的影响 总被引:1,自引:0,他引:1
研究了6个橡胶树品种幼苗(适应1年后)在不同生长光强(100%、50%、25%和5%自然光)下的叶片光合系统对光强和CO2浓度的响应特性。结果表明,6个橡胶树品种对不同的光环境均表现出较强的适应性。在不同生长光强下,橡胶树幼苗叶片的最大光合速率(Pmax)、光补偿点(LCP)、暗呼吸速率(Rd)、磷酸丙糖利用速率(TPU)、最大羧化速率(Vcmax)和最大电子传递速率(Jmax)以及叶绿素含量(Chl)均有显著差异(P<0.05),而光饱和点(LSP)和AQY(表观量子效率)则无显著差异。相同生长光强下,6个橡胶树品种间叶片的最大光合速率(Pmax)、暗呼吸速率(Rd)、磷酸丙糖利用速率(TPU)、最大电子传递速率(Jmax)和叶绿素含量(Chl)有显著差异(P<0.05),其光补偿点(LCP)、最大羧化速率(Vcmax)和表观量子效率(AQY)则无显著差异。综合比较各参数,RRIM600、云研77-4和PR107适宜于相对光强为100%~50%的植胶环境,而云研77-2、GT1和热研523适宜于相对光强为50%~25%的植胶环境。 相似文献
50.
Muhammad Aqib Fahim Nawaz Sadia Majeed Abdul Ghaffar Khawaja Shafique Ahmad Muhammad Asif Shehzad Muhammad Naeem Tahir Muhammad Aurangzaib Hafiz Muhammad Rashad Javeed Muhammad Habib-ur-Rahman Muhammad Munir Usmani 《Physiology and Molecular Biology of Plants》2021,27(5):1073
The present study involved two pot experiments to investigate the response of mung bean to the individual or combined SO42− and selenate application under drought stress. A marked increment in biomass and NPK accumulation was recorded in mung bean seedlings fertilized with various SO42− sources, except for CuSO4. Compared to other SO42− fertilizers, ZnSO4 application resulted in the highest increase in growth attributes and shoot nutrient content. Further, the combined S and Se application (S + Se) significantly enhanced relative water content (16%), SPAD value (72%), photosynthetic rate (80%) and activities of catalase (79%), guaiacol peroxidase (53%) and superoxide dismutase (58%) in the leaves of water-stressed mung bean plants. Consequently, the grain yield of mung bean was markedly increased by 105% under water stress conditions. Furthermore, S + Se application considerably increased the concentrations of P (47%), K (75%), S (80%), Zn (160%), and Fe (15%) in mung bean seeds under drought stress conditions. These findings indicate that S + Se application potentially increases the nutritional quality of grain legumes by stimulating photosynthetic apparatus and antioxidative machinery under water deficit conditions. Our results could provide the basis for further experiments on cross-talk between S and Se regulatory pathways to improve the nutritional quality of food crops.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12298-021-00992-6. 相似文献