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361.
Abstract The marine ciliate Strombidium purpureum Kahl harbours endosymbiotic purple non-sulphur bacteria. The bacteria contain bacteriochlorophyll a and the carotenoid spirilloxanthin, and they have photosynthetic membranes and cell walls. The ciliates require light for survival and growth under anaerobic conditions; in the dark the cells prefer microaerobic conditions. The ciliates show a photosensory behaviour, and they accumulate in light at wave lenghts corresponding to the absorption spectrum of the symbionts. The findings are discussed in terms of theories on the endosymbiotic origin of mitochondria. 相似文献
362.
A two-stage heminested PCR approach was developed to amplify small subunit (SSU) rDNA sequences, via two overlapping fragments, from single cells of microbial eucary-otes. The method was evaluated using the ciliate protozoon Spathidiutn when PCR products were obtained from nine of 10 cells tested. Southern blotting demonstrated that all fragments contained the same sequence in a region of SSU rDNA which is normally highly variable between species. A fluorescent oligonucleotide probe was used to demonstrate that this sequence also occurred in fixed cells of Spathidiutn. Fixatives containing mercuric salts preserved cell shape and allowed probe binding with little background auto-fluorescence. The Spathidiutn sequence is closely related to that from the haptorid Homalozoon vermiculare. 相似文献
363.
NELSON R. COOLEY JAMES M. KELTNER JERROLD FORESTER 《The Journal of eukaryotic microbiology》1972,19(4):636-638
Effects of 2 toxicants, Mirex and Aroclor 1254, on Tetrahymena pyriformis strain W in axenic cultures were investigated. Mirex is a chlorinated hydrocarbon effective against the fire ant, and Aroclor 1254 is a compound structurally related to DDT and used extensively in various industrial processes. Both toxicants reduced growth rates and population densities of T. pyriformis grown at 26 C generally in proportion to concentrations of the chemicals, their effects becoming statistically significant (P < 0.05) at 0.9 μg/liter for Mirex and 1.0 and 10.0 μg/liter for Aroclor 1254. Ciliates exposed to the toxicants for 7 days concentrated Mirex 193 × and Aroclor 60 × as compared to the initial concentrations of these compounds. It is suggested that the chief effect of the 2 toxicants on populations of T. pyriformis and of similarly responding ciliates in nature would be to reduce the availability of these protozoa as food organisms and nutrient regenerators. The ability of the ciliates to concentrate the tested compounds would permit the toxicants to enter into and to be translocated through aquatic food chains. In this manner the compounds could exert toxic effects at higher trophic levels. 相似文献
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367.
Pablo Quintela‐Alonso Frank Nitsche Claudia Wylezich Hartmut Arndt Wilhelm Foissner 《The Journal of eukaryotic microbiology》2013,60(3):235-246
The identification of species within the genus Tetrahymena is known to be difficult due to their essentially identical morphology, the occurrence of cryptic and sibling species and the phenotypic plasticity associated with the polymorphic life cycle of some species. We have combined morphology and molecular biology to describe Tetrahymena aquasubterranea n. sp. from groundwater of Cape Town, Republic of South Africa. The phylogenetic analysis compares the cox1 gene sequence of T. aquasubterranea with the cox1 gene sequences of other Tetrahymena species and uses the interior‐branch test to improve the resolution of the evolutionary relationships. This showed a considerable genetic divergence of T. aquasubterranea to its next relative, T. farlyi, of 9.2% (the average cox1 divergence among bona fide species of Tetrahymena is ~ 10%). Moreover, the analysis also suggested a sister relationship between T. aquasubterranea and a big clade comprising T. farleyi, T. tropicalis, T. furgasoni and T. mobilis. The morphological data available for these species show that they share with T. aquasubterranea a pyriformis‐like life style and at least two of them, T. farleyi and T. mobilis, a similar type II silverline pattern consisting of primary and secondary meridians. Tetrahymena aquasubterranea exhibits a biphasic life cycle with trophonts and theronts, is amicronucleate, and feeds on bacteria. 相似文献
368.
Ciliated Protozoan communities in a fluvial ecosystem 总被引:2,自引:2,他引:0
The ciliated protozoan communities in the Llobregat River (Barcelona, Spain) were studied for one year. Samples were taken from 11 different stations. The physical-chemical factors of the water as well as the qualitative and quantitative nature of the ciliate populations were considered. 105 species of ciliates, grouped in 13 orders, were found.The species which constantly appeared at all 11 stations (although not every month) were Litonotus fasciola, Trithigmostoma cucullulus, Cyclidium glaucoma, Glaucoma scintillans, Vorticella microstoma, Oxytricha sp., Stylonychia mytilus, Aspidisca costata and Aspidisca lynceus. The index of diversity at the different stations, and the affinity between the most abundant species, were also determined. 相似文献
369.
In the shallow eutrophic waters of the Darss-Zingst-ecosystem (Baltic Sea) wind induced mixing of the water column, and resuspension
of sediment is an important phenomenon. Incubation experiments (7 days) with natural water were performed in the laboratory
under simulated mixing conditions in order to assess the effects on abundance, biomass and activity of bacteria, heterotrophic
nanoflagellates and ciliates in the water column and upper bottom layers.
Under calm mixing conditions a bottom layer developed, rich in organic aggregates. Bacterial biomass increased through increase
of bacteria abundance and doubled cell volume. Consequently, biomass of flagellates and, more pronounced, of ciliates increased.
The bottom layer, comprising only 15% of the incubated volume, containedca. 60% of the bacterial biomass and ca. 55% of production, 75 and 95% of flagellate biomass, and ca. 70% of ciliate biomass.
It is concluded that bottom layers rich in organic aggregates, although not being present permanently, play an important role
in the shallow Darss-Zingst-ecosystem. 相似文献
370.
Summary The nuclear apparatus ofRemanella granulosa has been investigated using conventional TEM methods and Bernhard's technique of preferential RNP staining. This species has two (rarely three) macronuclei and a single micronucleus (rarely two micronuclei). The nuclei always form a single group.The macronuclei contain a fibro-granular matrix resistant to EDTA destaining, and several nucleoli and chromatin bodies. The chromatin bodies are readily bleached with EDTA and are often clustered, or even fused, forming chromocenters. The nuclei are of the compact concentric type. Some macronuclei contain nuclear bodies, as finely fibrous spheres or bundles of coarse fibers, or both. Neither type of nuclear body is destained with EDTA. The spheres are frequently associated with nucleoli. There is no evidence of any transition between the two types of nuclear bodies. The macronuclear envelope contains numerous pore complexes and is strengthened with an electron dense layer. The micronucleus is filled with spongy condensed chromatin and surrounded by an envelope with occasional pores. This nucleus lacks nucleoli and nuclear bodies. 相似文献