七氟醚联合骶管阻滞麻醉对小儿疝气手术的麻醉效果分析

目的:观察七氟醚联合骶管阻滞麻醉对小儿疝气手术的麻醉效果。方法:选取80例行腹股沟疝气手术患儿,按随机数字表法分为两组,对照组(39例)静脉注射氯胺酮,观察组(41例)先吸入8%七氟醚,然后进行骶管阻滞麻醉,通过观察并记录两组患儿生命体征、麻醉诱导时间、苏醒时间、手术麻醉时间、苏醒期躁动评分(Pediatric anesthesia emergence delirium,PAED)和麻醉诱导期合作量表(Induction Compliance Checklist,ICC)及麻醉期间不良反应情况,评价七氟醚联合骶管阻滞麻醉对小儿疝气手术的麻醉效果。结果:两组切皮后T1、T2时心率(HR)、平均动脉压(MAP)水平均高于T0时的值(P0.05),两组切皮后T1、T2时组间HR、MAP水平相比,无统计学差异(P0.05)。两组切皮后T3时HR、MAP水平基本恢复到T0时的水平。两组切皮前后4个时间点的血氧饱和度(Sp O2)相比,无统计学差异(P0.05)。观察组患儿麻醉诱导时间,苏醒时间均明显短于对照组患儿(P0.05),两组术中麻醉持续时间相比,无统计学差异(P0.05),均能达到期望麻醉时间,观察组患儿PAED评分和ICC评分均低于对照组患儿(P0.05),不良反应组间比较无统计学差异(P0.05)。结论:七氟醚联合骶管阻滞麻醉对小儿疝气手术具有良好的麻醉效果,麻醉诱导快,苏醒快,小儿配合度高,术后躁动少,值得临床推广使用。     

Determining spatio-temporal distribution of bee forage species of Al-Baha region based on ground inventorying supported with GIS applications and Remote Sensed Satellite Image analysis

In arid zones, the shortage of bee forage is critical and usually compels beekeepers to move their colonies in search of better forages. Identifying and mapping the spatiotemporal distribution of the bee forages over given area is important for better management of bee colonies. In this study honey bee plants in the target areas were inventoried following, ground inventory work supported with GIS applications. The study was conducted on 85 large plots of 50 × 50 m each. At each plot, data on species name, height, base diameter, crown height, crown diameter has been taken for each plant with their respective geographical positions. The data were stored, and processed using Trimble GPS supported with ArcGIS10 software program. The data were used to estimate the relative frequency, density, abundance and species diversity, species important value index and apicultural value of the species. In addition, Remotely Sensed Satellite Image of the area was obtained and processed using Hopfield Artificial Neural Network techniques. During the study, 182 species from 49 plant families were identified as bee forages of the target area. From the total number of species; shrubs, herbs and trees were accounting for 61%, 27.67%, and 11.53% respectively. Of which Ziziphus spina-christi, Acacia tortilis, Acacia origina, Acacia asak, Lavandula dentata, and Hypoestes forskaolii were the major nectar source plants of the area in their degree of importance. The average vegetation cover values of the study areas were low (<30%) with low Shannon’s species diversity indices (H′) of 0.5–1.52 for different sites. Based on the eco-climatological factors and the variations in their flowering period, these major bee forage species were found to form eight distinct spatiotemporal categories which allow beekeepers to migrate their colonies to exploit the resources at different seasons and place. The Remote Sensed Satellite Image analysis confirmed the spatial distribution of the bee forage resources as determined by the ground inventory work. An integrated approach, combining the ground inventory work with GIS and satellite image processing techniques could be an important tool for characterizing and mapping the available bee forage resources leading to their efficient and sustainable utilization.     

"基因电脑克隆"软件SiClone的并行优化研究与实现

生物信息学中,发现、鉴别新基因是承上启下的一步,它既承接了过往如“基因组测序”的工作,又是未来“后基因时代”研究的基石．“基因电脑克隆”是利用计算手段发现、鉴别新基因的方法,SiClone软件实现了“基因电脑克隆”功能．本文对SiClone软件操作的数据库提出并行处理方案,并详述了基于MPI(message passing interface)平台实现的并行优化版本PSiClone．根据已得到的EST数据库,展示了软件并行版PSiClone的运行性能,试验数据库EST序列条数仅仅是NCBI(The National Center for Biotechnology Information)dbEST庞大数据库的很小部分,这也暗示我们软件的并行工作对于大数据库的比较和运算将更有应用前景．     

黄土高原不同降水区休闲期土壤贮水效率及其对冬小麦水分利用的影响

土壤贮水是影响黄土高原冬小麦生产力的最重要因素,分析休闲期贮水效率对有效利用水资源具有重要意义。利用黄土高原旱作区4个农业气象观测站土壤水分长期观测资料和冬小麦产量资料,探讨了不同气候区休闲期土壤贮水和耗水特征及对冬小麦水分利用的影响。结果表明:(1)黄土高原旱作区休闲期1 m土层多年平均贮水量半湿润区为9 1mm,贮水效率为30.7%,半干旱区为32 mm,贮水效率为16.5%,且不同降水年型、不同气候区休闲期贮水量和贮水效率差别较大;(2)黄土高原旱作区1 m土层贮水量从土壤解冻至封冻期间基本呈波谷型分布,休闲期为主要贮水阶段,冬小麦返青—开花期为休闲期贮水的主要消耗阶段。半湿润区休闲期土壤贮水量主要消耗在起身至开花期,半干旱区主要消耗在越冬至拔节期;(3)黄土高原旱作区播种—越冬前消耗0—40 cm土层贮水,越冬-起身期各土层贮水量都有消耗,起身—开花期半湿润区主要消耗0—40 cm土层贮水量、半干旱区主要消耗0—60 cm土层贮水量,开花—成熟期半湿润区主要消耗40 mm以下土层贮水量、半干旱区主要消耗60 cm以下土层贮水量;(4)黄土高原休闲期贮水效率与冬小麦产量显著相关,半湿润区水分利用效率远高于半干旱区。黄土高原不同区域降水时空分布不均和土壤贮水能力的差异是造成不同气候区休闲期水分贮存差异的主要原因,通过调整耕作方式、水肥管理、种植结构进一步实现冬小麦增产和水分高效利用。     

2007－2012 年佛冈县稻飞虱灯下发生期及种群数量动态分析

明确佛冈县稻飞虱灯下发生期及灯诱种群数量动态,对当地稻飞虱的预测预报和防治工作具有重要意义.根据佛冈县2007-2012年白背飞虱和褐飞虱的灯诱数据,分析了该县白背飞虱和褐飞虱的灯下始见日、终见日、发生期及灯诱量等.结果表明在佛冈地区,白背飞虱和褐飞虱混合发生;白背飞虱的灯下始见日集中在3月底至4月上旬,明显比褐飞虱早一个月,终见日均在10月下旬,差别不大;稻飞虱在早稻和晚稻期间均会出现大小不一的灯诱高峰,早稻期间白背飞虱高峰期集中在5月中旬至6月中旬,褐飞虱灯诱高峰期较迟,主要在7月中旬前后;晚稻期间向背飞虱高峰期大多在9月上旬至10月上旬,褐飞虱高峰期在10月份;早稻期间,白背飞虱的灯诱量占绝对优势,晚稻期间褐飞虱的灯诱量上升,与白背飞虱灯诱量基本持平.因此,佛冈县稻飞虱的灯下发生期及种群数量动态在一定程度上可反映当地稻飞虱的发生情况.     

BDNF and trkB mRNA Expression in Neurons of the Neonatal Mouse Barrel Field Cortex: Normal Development and Plasticity after Cauterizing Facial Vibrissae

Development of the central somatosensory system is profoundly modulated by the sensory periphery. Cauterization of facial whiskers alters the segregation pattern of barrels in rodents only during a few days just after birth (critical period). Although a molecular basis of the segregation of barrel neurons and the critical period for the anatomical plasticity observed in layer IV barrel neuron is not clear yet, the accumulating evidence suggests that neurotrophins modulate synaptic connections including central nervous system. In this study, we showed by in situ hybridization that mouse barrel side neurons express brain-derived neurotrophic factor (BDNF) mRNA and both catalytic and non-catalytic forms of trkB mRNA. Cautery of row C vibrissae on the right side of the face within 24 h after birth (post natal day 0, PND0) reduced the expression of BDNF and trkB mRNA from the division region between the contralateral row C barrels at PND7. The vibrissae in row A, C, and E were cauterized at PND0 followed by quantitative RT-PCR for BDNF and trkB mRNA with total RNA isolated from the barrel region at PND7. The result showed that BDNF, but not trkB, mRNA was increased several-fold in the contralateral barrel region. These data suggest that the expression of BDNF mRNA is differentially regulated between injured barrels and actively innervated barrels. The differential expression of the mRNA encoding neurotrophins and their receptors may be important in regulating the injury-dependent re-segregation of barrels.     

Testing the suitability of insect orders as indicators for olive farming systems

• 1 A previous study suggested the use of certain insects groups as indicators for detecting organic olive farming in Southern Spain. To validate the use of these groups, insects were collected from olive orchards in Cordoba and Granada, comprising two Andalusian provinces with different surrounding landscapes.
• 2 Canopies were sampled using the branch‐beating technique during pre‐blooming and post‐blooming periods over 3 years in Granada (1999, 2000 and 2003) and 1 year in Cordoba (2003).
• 3 Using a nonparametric linear discriminant analysis method, based on the k‐nearest neighbour algorithm, two discriminant functions were constructed. A first discriminant model took into account interannual variability in Granada Province and the second model focused on environmental heterogeneity between the two provinces. Cross‐validation techniques, such as leave‐one‐out and split‐sample, were applied to the associated discriminant functions for each model to check their performance.
• 4 Even though differences existed with respect to the insect composition of the regions, the second model correctly classified 78.1% of the sampled blocks under the non‐organic and organic farming systems at the same time as taking into account two orders: Coleoptera and Hemiptera [excluding Euphyllura olivina olivina (Psyllidae) and the Heteroptera suborder]. The results suggest that the relative abundance of these groups in the post‐blooming period could constitute a potential bio‐indicator of organic olive farming system.

     

183例早产儿围产期高危因素及并发症分析

目的：探讨早产儿发生的围产期高危因素及并发症。方法：收集我院2006．1—2009．12出生的早产儿183例的临床资料,总结其早产的相关围产期高危因素及并发症。结果：胎膜早破、多胎妊娠、妊娠高血压综合征是早产发生的主要原因;早产儿的主要并发症为肺炎和颅内出血等。结论：加强围生期保健,早期干预各种围产期高危因素,积极防治早产儿各种并发症,是提高早产儿存活率降低致残率的关键。     

环青海湖地区天然草地时序光谱特征参量分析

获取了环青海湖地区4类主要天然草地2003年5~10月共16个时相的地面高分辨率光谱数据,并利用植被指数技术、导数光谱技术、植被光谱维特征提取模型及包络线归一化技术提取了多个光谱特征参量,通过对各参量在生育期内分布规律的分析,给出了能较好地表征草地生长发育规律时序特征参量的具体分布;最后计算了4类天然草地各时序特征参量的平均散度,结果表明黄边位置λY、红边位置λV、红边斜率SV、绿峰半高宽wλG、红谷半高宽wλR、去包络红谷净面积AR'和归一化植被指数NDV I对于天然草地分类更有效。