Radiofrequency ablation (RFA) is a widely used treatment for atrial fibrillation, the most common cardiac arrhythmia. Here, we explore autofluorescence hyperspectral imaging (aHSI) as a method to visualize RFA lesions and interlesional gaps in the highly collagenous left atrium. RFA lesions made on the endocardial surface of freshly excised porcine left atrial tissue were illuminated by UV light (365 nm), and hyperspectral datacubes were acquired over the visible range (420–720 nm). Linear unmixing was used to delineate RFA lesions from surrounding tissue, and lesion diameters derived from unmixed component images were quantitatively compared to gross pathology. RFA caused two consistent changes in the autofluorescence emission profile: a decrease at wavelengths below 490 nm (ascribed to a loss of endogenous NADH) and an increase at wavelengths above 490 nm (ascribed to increased scattering). These spectral changes enabled high resolution, in situ delineation of RFA lesion boundaries without the need for additional staining or exogenous markers. Our results confirm the feasibility of using aHSI to visualize RFA lesions at clinically relevant locations. If integrated into a percutaneous visualization catheter, aHSI would enable widefield optical surgical guidance during RFA procedures and could improve patient outcome by reducing atrial fibrillation recurrence.
There is a huge interest in developing strategies to effectively eliminate biofilms due to their negative impact in both industrial and clinical settings. In this study, structural damage was induced on two day‐old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. Radiant exposure of 225 mJ/cm2 induced distinct changes on the surface structure and overall morphology of the matured biofilms after laser irradiation. Moreover, at the radiant exposure used, changes in the colour and viscosity of the biofilm were observed which may indicate a compromised extracellular matrix. Irradiated biofilms in the presence of gold film also showed strong propidium iodide signal which implies an increase in the number of dead bacterial cells after laser treatment. Thus, this laser‐based technique is a promising approach in targeting and eradicating matured biofilms attached on surfaces such as medical implants.
目的:分析超声引导下微波消融术治疗良性甲状腺结节的疗效及安全性。方法:选择2013年1月-2015年1月入住我院治疗的100例良性甲状腺结节患者,分成微波消融术组、常规手术组进行治疗。对2组患者术前后的血清指标(IL-6、CRP、TNF-α)进行检测和比较,并比较两组患者的手术时间、术中出血量、术后住院时间及并发症的发生情况。结果:两组患者术后的血清CRP、IL-6、TNF-α水平均较术前升高,但超声引导微波消融手术前后的CRP及IL-6变化程度不大(P0.05),术后TNF-α水平低于传统手术组(P0.05)。微波消融手术出血量(12.34±0.99 m L)比传统手术出血量(28.75±2.15 m L)少,手术时间(28.46±2.63 min)比传统手术(84.05±6.95 min)短,术后住院时间(1.45±0.43 d)比常规手术(3.5±0.41 d)短,差异均具有统计学意义(P0.05)。两组患者术后并发症发生率比较差异无统计学意义(P0.05)。结论:超声引导下微波消融治疗甲状腺良性结节的疗效肯定,安全性高,对机体创伤较小,无手术瘢痕。 相似文献
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